ZSWIM8 (NM_001242488) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	KIAA0913
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235346 representing NM_001242488 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGATGTTTGCAGAGTGGGAGGACGGAGAGCGCTTCTCATTCGAGGATTCGGACCGTTTTGAGG AGGATTCACTCTGTTCCTTCATCTCCGAGGCCGAGAGCCTCTGCCAGAACTGGCGGGGATGGCGCAAACA GTCAGCGGGGCCCAATTCCCCCACTGGCGGCGGTGGCGGAGGTGGCAGTGGCGGTACCAGAATGCGAGAT GGACTGGTGATCCCATTGGTGGAGCTGTCAGCAAAGCAGGTGGCATTTCATATCCCATTTGAAGTGGTGG AGAAAGTTTACCCACCAGTGCCTGAGCAGCTACAGCTCCGAATTGCTTTTTGGAGCTTCCCTGAGAATGA AGAGGACATTCGGCTGTATTCGTGCCTGGCCAATGGCAGTGCGGATGAGTTTCAGCGAGGGGATCAGCTC TTCCGCATGCGGGCTGTGAAGGACCCATTGCAGATAGGGTTCCACCTGAGTGCTACAGTGGTGCCACCTC AGATGGTCCCTCCTAAAGGGGCCTACAACGTGGCTGTGATGTTTGACCGCTGCCGGGTCACTTCCTGCAG CTGTACCTGTGGGGCTGGGGCCAAATGGTGCACCCACGTCGTGGCACTCTGTCTCTTCCGCATCCACAAC GCTTCTGCAGTCTGCCTGCGAGCCCCAGTCTCAGAGTCCCTGTCCCGGCTACAGAGGGACCAGCTGCAAA AGTTTGCTCAGTACCTCATCAGTGAGCTCCCTCAGCAGATCCTCCCCACAGCTCAGCGTCTCCTGGACGA ACTCCTGTCTTCCCAGTCAACAGCCATCAATACAGTGTGTGGAGCTCCGGACCCCACAGCAGGGCCCTCA GCATCGGACCAGAGTACTTGGTATCTGGATGAATCGACACTCACTGACAACATCAAAAAGACACTGCACA AGTTCTGTGGCCCCTCCCCTGTGGTCTTCAGTGATGTGAACTCCATGTATCTGTCTTCCACGGAGCCGCC AGCCGCTGCTGAATGGGCATGTCTGCTGCGCCCTCTGAGGGGCCGTGAGCCAGAGGGCGTCTGGAACCTG CTAAGCATTGTGCGGGAGATGTTCAAGCGGAGGGACAGCAATGCTGCCCCCTTGTTGGAAATCCTCACTG ACCAGTGCCTCACCTATGAACAGATAACAGGTTGGTGGTATAGCGTACGTACCTCAGCCTCACACAGCAG TGCCAGTGGGCACACGGGCCGTAGCAACGGGCAGTCAGAGGTGGCAGCCCATGCCTGTGCCAGCATGTGT GACGAGATGGTCACACTGTGGAGGCTGGCCGTGCTGGACCCTGCACTCAGCCCCCAGCGGCGCCGGGAAC TGTGTACGCAGCTGCGGCAGTGGCAACTGAAGGTGATTGAGAACGTCAAGCGGGGCCAACACAAGAAGAC GCTGGAGCGGCTCTTCCCCGGCTTCCGGCCAGCGGTGGAGGCCTGCTACTTCAACTGGGAAGAGGCCTAC CCACTTCCTGGTGTCACCTACAGCGGCACTGACAGGAAGCTGGCACTGTGCTGGGCCCGGGCCCTGCCCT CTCGGCCAGGTGCCTCCCGCTCTGGGGGCCTGGAGGAATCCCGGGACCGGCCCCGACCCCTTCCTACTGA GCCAGCTGTGCGGCCCAAGGAGCCTGGGACCAAGCGAAAGGGCTTGGGTGAGGGGGTCCCCTCATCACAG CGGGGTCCCCGCCGCCTCTCAGCTGAAGGGGGAGATAAAGCTCTACATAAGATGGGTCCAGGTGGGGGCA AAGCCAAGGCACTGGGTGGGGCTGGCAGTGGGAGCAAGGGCTCAGCAGGTGGCGGAAGCAAGCGACGGCT GAGCAGCGAAGACAGCTCCCTGGAGCCAGACCTGGCCGAGATGAGCCTGGATGACAGCAGCCTGGCCCTG GGCGCAGAGGCCAGCACCTTCGGGGGATTCCCTGAGAGCCCTCCACCCTGTCCTCTCCACGGTGGCTCCC GAGGCCCTTCCACTTTCCTTCCTGAGCCCCCAGATACTTATGAAGAAGATGGTGGTGTGTACTTCTCGGA AGGGCCTGAGCCTCCCACAGCCTCTGTTGGCCCCCCTGGCCTACTGCCTGGGGATGTCTGTACCCAGGAC GACCTCCCTTCTACAGATGAGAGTGGCAATGGGCTTCCCAAAACCAAAGAGGCAGCCCCTGCAGTTGGAG AGGAGGATGATGACTACCAGGCGTACTATCTGAATGCCCAGGATGGGGCTGGGGGCGAGGAAGAGAAGGC CGAGGGCGGGGCTGGGGAGGAGCACGACCTGTTTGCTGGGCTGAAGCCACTGGAACAGGAGAGTCGCATG GAGGTACTGTTTGCCTGTGCTGAGGCCCTGCATGCGCATGGCTATAGCAGTGAGGCCTCCCGTCTCACTG TGGAGCTTGCCCAGGATCTGCTAGCCAACCCACCCGACCTCAAGGTAGAGCCGCCCCCTGCCAAGGGCAA GAAGAACAAGGTATCCACGAGCCGTCAGACCTGGGTGGCTACCAACACCCTGAGCAAGGCGGCCTTCCTG TTGACAGTGCTAAGTGAGCGTCCAGAGCACCACAACCTGGCCTTCCGAGTTGGCATGTTTGCCTTGGAGC TACAGAGGCCTCCAGCTTCTACCAAGGCCTTGGAGGTGAAGCTGGCATACCAGGAGTCTGAGGTGGCTGC CCTGCTCAAGAAGATCCCTCTGGGTCCAAGTGAGATGAGTACCATGCGGTGCCGGGCAGAGGAACTTCGG GAGGGGACACTCTGTGACTATCGGCCTGTGTTGCCTCTCATGCTGGCCAGTTTCATCTTTGACGTTCTCT GTGCTCCAGGTTCCCGGCCCCCAAGTCGCAACTGGAACAGCGAGACACCTGGGGATGAGGAGCTTGGATT TGAAGCAGCAGTTGCTGCCTTGGGCATGAAGACAACAGTGAGCGAGGCAGAACATCCCCTCTTATGTGAA GGCACACGTCGGGAGAAGGGTGACCTGGCATTAGCACTAATGATCACTTACAAGGACGACCAGGCCAAGC TTAAGAAGATCTTAGACAAACTCTTGGACCGAGAGAGCCAGACACATAAGCCACAGACGCTGAGTTCTTT CTACTCATCTAGCCGCCCAACCACAGCCAGCCAGAGGTCTCCTTCAAAGCACGGGGGCCCATCTGCCCCA GGGGCCCTGCAACCACTGACCTCAGGCTCTGCAGGGCCTGCTCAACCAGGGAGTGTGGCAGGGGCTGGGC CAGGCCCCACTGAGGGCTTCACAGAGAAGAATGTGCCTGAGAGTTCCCCACATTCCCCCTGTGAGGGTCT TCCATCTGAGGCAGCTTTGACCCCAAGGCCAGAAGGGAAGGTTCCTAGCCGCTTGGCACTTGGCAGTCGT GGAGGCTATAATGGACGGGGATGGGGGTCCCCAGGACGGCCTAAGAAGAAGCACACAGGCATGGCCAGCA TTGACAGCAGTGCCCCTGAAACAACATCGGATAGTTCCCCCACCTTAAGCCGGAGACCACTTCGAGGGGG CTGGGCCCCCACCTCCTGGGGTCGAGGTCAGGACAGTGACAGCATTAGCAGCTCTTCTTCGGACTCCCTG GGCTCCTCATCCTCCAGTGGAAGTCGCCGGGCCAGTGCCAGTGGAGGAGCCCGGGCGAAGACTGTTGAAG TTGGCAGGTACAAGGGCCGCCGCCCCGAGAGTCATGCCCCTCATGTACCCAATCAGCCATCAGAGGCAGC TGCACACTTCTACTTCGAGCTGGCGAAGACAGTGCTGATCAAGGCAGGGGGCAACAGCAGCACTTCCATT TTCACACATCCATCTTCCTCAGGGGGCCACCAGGGTCCTCACCGCAACCTGCACCTTTGCGCCTTCGAGA TTGGGCTTTATGCCCTTGGCCTGCACAACTTTGTTTCTCCCAACTGGCTCTCACGTACTTATTCTTCCCA CGTTTCCTGGATTACAGGCCAGGCCATGGAGATAGGCAGCGCAGCCCTGACTATACTGGTAGAATGCTGG GATGGGCACCTGACACCCCCTGAGGTTGCATCCCTGGCTGACAGGGCATCACGGGCAAGAGACTCCAATA TGGTGAGGGCGGCAGCAGAGCTGGCCCTGAGCTGCCTGCCTCACGCCCATGCATTGAACCCTAATGAGAT CCAGCGGGCCCTGGTGCAGTGCAAGGAACAGGACAACCTGATGTTGGAGAAGGCCTGCATGGCAGTGGAA GAGGCAGCTAAGGGTGGGGGCGTGTACCCTGAAGTGTTGTTTGAGGTTGCTCACCAGTGGTTCTGGCTGT ATGAGCAAACTGCAGGTGGCTCATCCACAGCCCGTGAAGGGGCTACAAGCTGTAGTGCCAGTGGGATCAG GGCAGGTGGGGAAGCTGGGCGGGGTATGCCTGAGGGTAGAGGGGGCCCAGGGACTGAGCCGGTTACAGTG GCAGCGGCAGCAGTGACAGCAGCAGCCACAGTGGTGCCCGTCATATCGGTGGGGTCTAGTTTATACCCGG GTCCAGGACTGGGGCATGGCCACTCCCCTGGCCTGCACCCCTACACTGCTCTACAGCCCCACCTGCCCTG TAGCCCTCAGTATCTCACTCACCCAGCTCACCCTGCCCACCCCATGCCTCACATGCCCCGGCCTGCCGTC TTCCCTGTGCCCAGCTCTGCATACCCACAGGGTGTGCATCCTGCATTCCTAGGGGCTCAGTACCCTTATT CAGTGACTCCTCCCTCACTTGCTGCCACTGCTGTGTCTTTCCCCGTTCCTTCCATGGCACCCATCACAGT ACATCCCTACCACACAGAGCCAGGGCTTCCACTGCCCACCAGTGTGGCCTTGAGCAGTGTCCATCCAGCA TCCACGTTTCCAGCCATCCAAGGTGCCTCACTGCCTGCCCTGACCACACAGCCCAGCCCTCTGGTGAGCG GAGGTTTTCCACCGCCCGAGGAGGAGACACACAGTCAGCCAGTCAATCCCCACAGCCTGCACCACCTGCA TGCTGCCTACCGTGTCGGAATGCTGGCACTGGAGATGCTGGGTCGCCGGGCACACAACGATCACCCCAAC AACTTCTCCCGCTCCCCCCCCTACACTGATGATGTCAAATGGTTGCTGGGGCTGGCAGCAAAGCTGGGAG ATCGTCATGGAGACGCTGCAGCGGCTGAGTCCCGCTCATGCCCACAACCACCTGCGTGCCCCGGCCTTCC ACCAACTGGTGCAGCGCTGCCAGCAGGCATACATGCAGTACATCCACCACCGCTTGATTCACCTGACTCC TGCGGACTACGACGACTTTGTGAATGCGATCCGGAGTGCCCGCAGCGCCTTCTGCCTGACGCCCATGGGC ATGATGCAGTTCAACGACATCCTACAGAACCTCAAGCGCAGCAAACAGACCAAGGAGCTGTGGCAGCGGG TCTCACTCGAGATGGCCACCTTCTCCCCCTGAGTCTTTCACCCTTAGGGTCCTATACAGGGACCCAGGCC TGTGGCTATGGGGGCCCCTCACACAGGGGGAGTGAAACTTGGCTGGACAGATCATCCTCACTCAGTTCCC TGGTAGCACAGACTGACAGCTGCTCTTGGGCTATAGCTTGGGGCCAAGATGTCTCACACCCTAGAAGCCT AGGGCTGGGGGAGACAGCCCTGTCTGGGAGGGGGCGTTGGGTGGCCTCTGGTATTTATTTGGCATTTATA AATATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235346 representing NM_001242488 Red=Cloning site Green=Tags(s) MELMFAEWEDGERFSFEDSDRFEEDSLCSFISEAESLCQNWRGWRKQSAGPNSPTGGGGGGGSGGTRMRD GLVIPLVELSAKQVAFHIPFEVVEKVYPPVPEQLQLRIAFWSFPENEEDIRLYSCLANGSADEFQRGDQL FRMRAVKDPLQIGFHLSATVVPPQMVPPKGAYNVAVMFDRCRVTSCSCTCGAGAKWCTHVVALCLFRIHN ASAVCLRAPVSESLSRLQRDQLQKFAQYLISELPQQILPTAQRLLDELLSSQSTAINTVCGAPDPTAGPS ASDQSTWYLDESTLTDNIKKTLHKFCGPSPVVFSDVNSMYLSSTEPPAAAEWACLLRPLRGREPEGVWNL LSIVREMFKRRDSNAAPLLEILTDQCLTYEQITGWWYSVRTSASHSSASGHTGRSNGQSEVAAHACASMC DEMVTLWRLAVLDPALSPQRRRELCTQLRQWQLKVIENVKRGQHKKTLERLFPGFRPAVEACYFNWEEAY PLPGVTYSGTDRKLALCWARALPSRPGASRSGGLEESRDRPRPLPTEPAVRPKEPGTKRKGLGEGVPSSQ RGPRRLSAEGGDKALHKMGPGGGKAKALGGAGSGSKGSAGGGSKRRLSSEDSSLEPDLAEMSLDDSSLAL GAEASTFGGFPESPPPCPLHGGSRGPSTFLPEPPDTYEEDGGVYFSEGPEPPTASVGPPGLLPGDVCTQD DLPSTDESGNGLPKTKEAAPAVGEEDDDYQAYYLNAQDGAGGEEEKAEGGAGEEHDLFAGLKPLEQESRM EVLFACAEALHAHGYSSEASRLTVELAQDLLANPPDLKVEPPPAKGKKNKVSTSRQTWVATNTLSKAAFL LTVLSERPEHHNLAFRVGMFALELQRPPASTKALEVKLAYQESEVAALLKKIPLGPSEMSTMRCRAEELR EGTLCDYRPVLPLMLASFIFDVLCAPGSRPPSRNWNSETPGDEELGFEAAVAALGMKTTVSEAEHPLLCE GTRREKGDLALALMITYKDDQAKLKKILDKLLDRESQTHKPQTLSSFYSSSRPTTASQRSPSKHGGPSAP GALQPLTSGSAGPAQPGSVAGAGPGPTEGFTEKNVPESSPHSPCEGLPSEAALTPRPEGKVPSRLALGSR GGYNGRGWGSPGRPKKKHTGMASIDSSAPETTSDSSPTLSRRPLRGGWAPTSWGRGQDSDSISSSSSDSL GSSSSSGSRRASASGGARAKTVEVGRYKGRRPESHAPHVPNQPSEAAAHFYFELAKTVLIKAGGNSSTSI FTHPSSSGGHQGPHRNLHLCAFEIGLYALGLHNFVSPNWLSRTYSSHVSWITGQAMEIGSAALTILVECW DGHLTPPEVASLADRASRARDSNMVRAAAELALSCLPHAHALNPNEIQRALVQCKEQDNLMLEKACMAVE EAAKGGGVYPEVLFEVAHQWFWLYEQTAGGSSTAREGATSCSASGIRAGGEAGRGMPEGRGGPGTEPVTV AAAAVTAAATVVPVISVGSSLYPGPGLGHGHSPGLHPYTALQPHLPCSPQYLTHPAHPAHPMPHMPRPAV FPVPSSAYPQGVHPAFLGAQYPYSVTPPSLAATAVSFPVPSMAPITVHPYHTEPGLPLPTSVALSSVHPA STFPAIQGASLPALTTQPSPLVSGGFPPPEEETHSQPVNPHSLHHLHAAYRVGMLALEMLGRRAHNDHPN NFSRSPPYTDDVKWLLGLAAKLGDRHGDAAAAESRSCPQPPACPGLPPTGAALPAGIHAVHPPPLDSPDS CGLRRLCECDPECPQRLLPDAHGHDAVQRHPTEPQAQQTDQGAVAAGLTRDGHLLPLSLSPLGSYTGTQA CGYGGPSHRGSETWLDRSSSLSSLVAQTDSCSWAIAWGQDVSHPRSLGLGETALSGRGRWVASGIYLAFI NI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242488
ORF Size	5676 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242488.2
RefSeq Size	5970 bp
RefSeq ORF	5679 bp
Locus ID	23053
UniProt ID	A7E2V4
MW	202 kDa
Gene Summary	Substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex that promotes target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs) (PubMed:33184234, PubMed:33184237). The SCF-like E3 ubiquitin-protein ligase complex acts by catalyzing ubiquitination and subsequent degradation of AGO proteins (AGO1, AGO2, AGO3 and/or AGO4), thereby exposing miRNAs for degradation (PubMed:33184234, PubMed:33184237). Specifically recognizes and binds AGO proteins when they are engaged with a TDMD target (PubMed:33184234). May also acts as a regulator of axon guidance: specifically recognizes misfolded ROBO3 and promotes its ubiquitination and subsequent degradation (PubMed:24012004).[UniProtKB/Swiss-Prot Function]

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