ZSWIM8 (NM_001242488) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	KIAA0913
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331819 representing NM_001242488. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGCTGATGTTTGCAGAGTGGGAGGACGGAGAGCGCTTCTCATTCGAGGATTCGGACCGTTTTGAG GAGGATTCACTCTGTTCCTTCATCTCCGAGGCCGAGAGCCTCTGCCAGAACTGGCGGGGATGGCGCAAA CAGTCAGCGGGGCCCAATTCCCCCACTGGCGGCGGTGGCGGAGGTGGCAGTGGCGGTACCAGAATGCGA GATGGACTGGTGATCCCATTGGTGGAGCTGTCAGCAAAGCAGGTGGCATTTCATATCCCATTTGAAGTG GTGGAGAAAGTTTACCCACCAGTGCCTGAGCAGCTACAGCTCCGAATTGCTTTTTGGAGCTTCCCTGAG AATGAAGAGGACATTCGGCTGTATTCGTGCCTGGCCAATGGCAGTGCGGATGAGTTTCAGCGAGGGGAT CAGCTCTTCCGCATGCGGGCTGTGAAGGACCCATTGCAGATAGGGTTCCACCTGAGTGCTACAGTGGTG CCACCTCAGATGGTCCCTCCTAAAGGGGCCTACAACGTGGCTGTGATGTTTGACCGCTGCCGGGTCACT TCCTGCAGCTGTACCTGTGGGGCTGGGGCCAAATGGTGCACCCACGTCGTGGCACTCTGTCTCTTCCGC ATCCACAACGCTTCTGCAGTCTGCCTGCGAGCCCCAGTCTCAGAGTCCCTGTCCCGGCTACAGAGGGAC CAGCTGCAAAAGTTTGCTCAGTACCTCATCAGTGAGCTCCCTCAGCAGATCCTCCCCACAGCTCAGCGT CTCCTGGACGAACTCCTGTCTTCCCAGTCAACAGCCATCAATACAGTGTGTGGAGCTCCGGACCCCACA GCAGGGCCCTCAGCATCGGACCAGAGTACTTGGTATCTGGATGAATCGACACTCACTGACAACATCAAA AAGACACTGCACAAGTTCTGTGGCCCCTCCCCTGTGGTCTTCAGTGATGTGAACTCCATGTATCTGTCT TCCACGGAGCCGCCAGCCGCTGCTGAATGGGCATGTCTGCTGCGCCCTCTGAGGGGCCGTGAGCCAGAG GGCGTCTGGAACCTGCTAAGCATTGTGCGGGAGATGTTCAAGCGGAGGGACAGCAATGCTGCCCCCTTG TTGGAAATCCTCACTGACCAGTGCCTCACCTATGAACAGATAACAGGTTGGTGGTATAGCGTACGTACC TCAGCCTCACACAGCAGTGCCAGTGGGCACACGGGCCGTAGCAACGGGCAGTCAGAGGTGGCAGCCCAT GCCTGTGCCAGCATGTGTGACGAGATGGTCACACTGTGGAGGCTGGCCGTGCTGGACCCTGCACTCAGC CCCCAGCGGCGCCGGGAACTGTGTACGCAGCTGCGGCAGTGGCAACTGAAGGTGATTGAGAACGTCAAG CGGGGCCAACACAAGAAGACGCTGGAGCGGCTCTTCCCCGGCTTCCGGCCAGCGGTGGAGGCCTGCTAC TTCAACTGGGAAGAGGCCTACCCACTTCCTGGTGTCACCTACAGCGGCACTGACAGGAAGCTGGCACTG TGCTGGGCCCGGGCCCTGCCCTCTCGGCCAGGTGCCTCCCGCTCTGGGGGCCTGGAGGAATCCCGGGAC CGGCCCCGACCCCTTCCTACTGAGCCAGCTGTGCGGCCCAAGGAGCCTGGGACCAAGCGAAAGGGCTTG GGTGAGGGGGTCCCCTCATCACAGCGGGGTCCCCGCCGCCTCTCAGCTGAAGGGGGAGATAAAGCTCTA CATAAGATGGGTCCAGGTGGGGGCAAAGCCAAGGCACTGGGTGGGGCTGGCAGTGGGAGCAAGGGCTCA GCAGGTGGCGGAAGCAAGCGACGGCTGAGCAGCGAAGACAGCTCCCTGGAGCCAGACCTGGCCGAGATG AGCCTGGATGACAGCAGCCTGGCCCTGGGCGCAGAGGCCAGCACCTTCGGGGGATTCCCTGAGAGCCCT CCACCCTGTCCTCTCCACGGTGGCTCCCGAGGCCCTTCCACTTTCCTTCCTGAGCCCCCAGATACTTAT GAAGAAGATGGTGGTGTGTACTTCTCGGAAGGGCCTGAGCCTCCCACAGCCTCTGTTGGCCCCCCTGGC CTACTGCCTGGGGATGTCTGTACCCAGGACGACCTCCCTTCTACAGATGAGAGTGGCAATGGGCTTCCC AAAACCAAAGAGGCAGCCCCTGCAGTTGGAGAGGAGGATGATGACTACCAGGCGTACTATCTGAATGCC CAGGATGGGGCTGGGGGCGAGGAAGAGAAGGCCGAGGGCGGGGCTGGGGAGGAGCACGACCTGTTTGCT GGGCTGAAGCCACTGGAACAGGAGAGTCGCATGGAGGTACTGTTTGCCTGTGCTGAGGCCCTGCATGCG CATGGCTATAGCAGTGAGGCCTCCCGTCTCACTGTGGAGCTTGCCCAGGATCTGCTAGCCAACCCACCC GACCTCAAGGTAGAGCCGCCCCCTGCCAAGGGCAAGAAGAACAAGGTATCCACGAGCCGTCAGACCTGG GTGGCTACCAACACCCTGAGCAAGGCGGCCTTCCTGTTGACAGTGCTAAGTGAGCGTCCAGAGCACCAC AACCTGGCCTTCCGAGTTGGCATGTTTGCCTTGGAGCTACAGAGGCCTCCAGCTTCTACCAAGGCCTTG GAGGTGAAGCTGGCATACCAGGAGTCTGAGGTGGCTGCCCTGCTCAAGAAGATCCCTCTGGGTCCAAGT GAGATGAGTACCATGCGGTGCCGGGCAGAGGAACTTCGGGAGGGGACACTCTGTGACTATCGGCCTGTG TTGCCTCTCATGCTGGCCAGTTTCATCTTTGACGTTCTCTGTGCTCCAGGTTCCCGGCCCCCAAGTCGC AACTGGAACAGCGAGACACCTGGGGATGAGGAGCTTGGATTTGAAGCAGCAGTTGCTGCCTTGGGCATG AAGACAACAGTGAGCGAGGCAGAACATCCCCTCTTATGTGAAGGCACACGTCGGGAGAAGGGTGACCTG GCATTAGCACTAATGATCACTTACAAGGACGACCAGGCCAAGCTTAAGAAGATCTTAGACAAACTCTTG GACCGAGAGAGCCAGACACATAAGCCACAGACGCTGAGTTCTTTCTACTCATCTAGCCGCCCAACCACA GCCAGCCAGAGGTCTCCTTCAAAGCACGGGGGCCCATCTGCCCCAGGGGCCCTGCAACCACTGACCTCA GGCTCTGCAGGGCCTGCTCAACCAGGGAGTGTGGCAGGGGCTGGGCCAGGCCCCACTGAGGGCTTCACA GAGAAGAATGTGCCTGAGAGTTCCCCACATTCCCCCTGTGAGGGTCTTCCATCTGAGGCAGCTTTGACC CCAAGGCCAGAAGGGAAGGTTCCTAGCCGCTTGGCACTTGGCAGTCGTGGAGGCTATAATGGACGGGGA TGGGGGTCCCCAGGACGGCCTAAGAAGAAGCACACAGGCATGGCCAGCATTGACAGCAGTGCCCCTGAA ACAACATCGGATAGTTCCCCCACCTTAAGCCGGAGACCACTTCGAGGGGGCTGGGCCCCCACCTCCTGG GGTCGAGGTCAGGACAGTGACAGCATTAGCAGCTCTTCTTCGGACTCCCTGGGCTCCTCATCCTCCAGT GGAAGTCGCCGGGCCAGTGCCAGTGGAGGAGCCCGGGCGAAGACTGTTGAAGTTGGCAGGTACAAGGGC CGCCGCCCCGAGAGTCATGCCCCTCATGTACCCAATCAGCCATCAGAGGCAGCTGCACACTTCTACTTC GAGCTGGCGAAGACAGTGCTGATCAAGGCAGGGGGCAACAGCAGCACTTCCATTTTCACACATCCATCT TCCTCAGGGGGCCACCAGGGTCCTCACCGCAACCTGCACCTTTGCGCCTTCGAGATTGGGCTTTATGCC CTTGGCCTGCACAACTTTGTTTCTCCCAACTGGCTCTCACGTACTTATTCTTCCCACGTTTCCTGGATT ACAGGCCAGGCCATGGAGATAGGCAGCGCAGCCCTGACTATACTGGTAGAATGCTGGGATGGGCACCTG ACACCCCCTGAGGTTGCATCCCTGGCTGACAGGGCATCACGGGCAAGAGACTCCAATATGGTGAGGGCG GCAGCAGAGCTGGCCCTGAGCTGCCTGCCTCACGCCCATGCATTGAACCCTAATGAGATCCAGCGGGCC CTGGTGCAGTGCAAGGAACAGGACAACCTGATGTTGGAGAAGGCCTGCATGGCAGTGGAAGAGGCAGCT AAGGGTGGGGGCGTGTACCCTGAAGTGTTGTTTGAGGTTGCTCACCAGTGGTTCTGGCTGTATGAGCAA ACTGCAGGTGGCTCATCCACAGCCCGTGAAGGGGCTACAAGCTGTAGTGCCAGTGGGATCAGGGCAGGT GGGGAAGCTGGGCGGGGTATGCCTGAGGGTAGAGGGGGCCCAGGGACTGAGCCGGTTACAGTGGCAGCG GCAGCAGTGACAGCAGCAGCCACAGTGGTGCCCGTCATATCGGTGGGGTCTAGTTTATACCCGGGTCCA GGACTGGGGCATGGCCACTCCCCTGGCCTGCACCCCTACACTGCTCTACAGCCCCACCTGCCCTGTAGC CCTCAGTATCTCACTCACCCAGCTCACCCTGCCCACCCCATGCCTCACATGCCCCGGCCTGCCGTCTTC CCTGTGCCCAGCTCTGCATACCCACAGGGTGTGCATCCTGCATTCCTAGGGGCTCAGTACCCTTATTCA GTGACTCCTCCCTCACTTGCTGCCACTGCTGTGTCTTTCCCCGTTCCTTCCATGGCACCCATCACAGTA CATCCCTACCACACAGAGCCAGGGCTTCCACTGCCCACCAGTGTGGCCTTGAGCAGTGTCCATCCAGCA TCCACGTTTCCAGCCATCCAAGGTGCCTCACTGCCTGCCCTGACCACACAGCCCAGCCCTCTGGTGAGC GGAGGTTTTCCACCGCCCGAGGAGGAGACACACAGTCAGCCAGTCAATCCCCACAGCCTGCACCACCTG CATGCTGCCTACCGTGTCGGAATGCTGGCACTGGAGATGCTGGGTCGCCGGGCACACAACGATCACCCC AACAACTTCTCCCGCTCCCCCCCCTACACTGATGATGTCAAATGGTTGCTGGGGCTGGCAGCAAAGCTG GGAGATCGTCATGGAGACGCTGCAGCGGCTGAGTCCCGCTCATGCCCACAACCACCTGCGTGCCCCGGC CTTCCACCAACTGGTGCAGCGCTGCCAGCAGGCATACATGCAGTACATCCACCACCGCTTGATTCACCT GACTCCTGCGGACTACGACGACTTTGTGAATGCGATCCGGAGTGCCCGCAGCGCCTTCTGCCTGACGCC CATGGGCATGATGCAGTTCAACGACATCCTACAGAACCTCAAGCGCAGCAAACAGACCAAGGAGCTGTG GCAGCGGGTCTCACTCGAGATGGCCACCTTCTCCCCCTGAGTCTTTCACCCTTAGGGTCCTATACAGGG ACCCAGGCCTGTGGCTATGGGGGCCCCTCACACAGGGGGAGTGAAACTTGGCTGGACAGATCATCCTCA CTCAGTTCCCTGGTAGCACAGACTGACAGCTGCTCTTGGGCTATAGCTTGGGGCCAAGATGTCTCACAC CCTAGAAGCCTAGGGCTGGGGGAGACAGCCCTGTCTGGGAGGGGGCGTTGGGTGGCCTCTGGTATTTAT TTGGCATTTATAAATATATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001242488
Insert Size	5679 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242488.1
RefSeq Size	5970 bp
RefSeq ORF	5679 bp
Locus ID	23053
UniProt ID	A7E2V4
MW	201.5 kDa
Gene Summary	Substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex that promotes target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs) (PubMed:33184234, PubMed:33184237). The SCF-like E3 ubiquitin-protein ligase complex acts by catalyzing ubiquitination and subsequent degradation of AGO proteins (AGO1, AGO2, AGO3 and/or AGO4), thereby exposing miRNAs for degradation (PubMed:33184234, PubMed:33184237). Specifically recognizes and binds AGO proteins when they are engaged with a TDMD target (PubMed:33184234). May also acts as a regulator of axon guidance: specifically recognizes misfolded ROBO3 and promotes its ubiquitination and subsequent degradation (PubMed:24012004).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses two alternate splice sites in the 3' coding region with the latter resulting in a frameshift, compared to variant 1. It encodes isoform 3, which has a longer and distinct C-terminus, compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.