SAFB (NM_001201338) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HAP; HET; SAB-B1; SAF-B; SAF-B1; SAFB1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235032 representing NM_001201338 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGACTCTGTCAGGCCTAGGTGATTCTGGAGCGGCGGGCGCGGCGGCTCTGAGCTCCGCCTCGT CAGAGACCGGGACGCGGCGCCTCAGCGACCTGCGAGTGATCGATCTGCGGGCGGAGCTGAGGAAACGGAA TGTGGACTCGAGCGGCAACAAGAGCGTTTTGATGGAGCGGCTGAAGAAGGCAATTGAAGATGAAGGTGGT AATCCTGACGAAATTGAAATTACCTCCGAGGGAAACAAGAAAACATCAAAGAGGTCTAGCAAAGGGCGCA AACCAGAAGAAGAGGGTGTGGAAGATAACGGGCTGGAGGAAAACTCTGGGGATGGACAGGAGGATGTTGA GACCAGTCTGGAGAACTTGCAGGACATCGACATCATGGATATCAGTGTGTTGGATGAAGCAGAAATTGAT AATGGAAGCGTTGCAGATTGTGTCGAAGACGATGATGCTGATAACCTCCAGGAGTCCCTGTCGGATAGTA GAGAGCTAGTCGAGGGGGAAATGAAAGAGCTTCCGGAGCAGCTTCAGGAACATGCTATAGAGGACAAAGA AACTATAAACAATTTAGATACTTCATCATCTGACTTCACTATATTACAGGAAATTGAAGAGCCATCCCTG GAGCCAGAAAATGAGAAAATACTCGACATTTTGGGGGAAACTTGTAAATCTGAGCCAGTAAAAGAAGAAA GTTCCGAGCTGGAGCAGCCATTTGCACAGGACACAAGTAGCGTGGGGCCAGACAGAAAGCTTGCGGAGGA AGAGGACCTATTTGACAGCGCCCATCCGGAAGAGGGTGATTTAGATTTGGCCAGCGAGTCAACAGCACAC GCTCAGTCGAGCAAGGCAGACAGCCTGTTAGCGGTAGTGAAAAGGGAGCCCGCGGAGCAGCCAGGCGATG GCGAGAGGACGGACTGTGAGCCTGTAGGGCTAGAGCCGGCAGTTGAGCAGAGTAGTGCGGCCTCCGAGCT CGCGGAGGCCTCTAGCGAGGAGCTCGCAGAAGCACCCACGGAAGCCCCAAGCCCAGAAGCCAGAGATAGC AAAGAAGACGGGAGGAAGTTTGATTTTGACGCTTGTAATGAAGTCCCTCCGGCTCCTAAAGAGTCCTCAA CCAGTGAGGGCGCTGATCAGAAAATGAGTTCTCCCGAAGATGACTCGGATACAAAAAGGCTTTCCAAAGA GGAAAAGGGTCGCAGCAGTTGTGGTAGAAATTTCTGGGTTAGTGGACTCTCTTCTACAACCAGAGCTACA GATTTGAAGAATCTTTTCAGCAAATATGGGAAGGTGGTGGGCGCCAAGGTTGTGACAAATGCCCGGAGTC CTGGAGCTCGCTGTTACGGTTTTGTCACGATGTCCACAGCAGAAGAGGCCACAAAATGCATTAACCACCT GCACAAGACGGAGCTCCACGGAAAGATGATCTCCGTGGAGAAAGCCAAAAATGAACCTGTGGGAAAGAAA ACCTCTGACAAAAGAGACAGTGACGGGAAAAAGGAGAAGTCGAGCAACAGTGACAGATCTACAAACCTTA AGAGGGATGATAAATGTGACAGAAAAGATGATGCTAAGAAGGGTGACGACGGAAGTGGAGAAAAGAGTAA GGACCAAGATGATCAGAAACCTGGCCCCTCAGAGCGATCTCGAGCCACAAAGTCAGGAAGTCGAGGGACC GAACGGACTGTAGTAATGGATAAATCCAAAGGGGTGCCTGTGATTAGTGTAAAAACGTCCGGGTCCAAAG AGAGAGCTTCCAAAAGCCAGGATCGCAAATCAGCCAGCAGAGAGAAGCGGTCCGTCGTGTCCTTTGATAA GGTCAAGGAGCCTCGGAAGTCAAGAGACTCAGAGTCCCATAGCAGGGTGCGTGAACGCAGTGAACGCGAA CAACGCATGCAGGCGCAGTGGGAGCGCGAGGAGCGTGAGCGGCTGGAGATTGCCCGAGAGAGGCTGGCCT TCCAGCGCCAGCGGCTGGAGCGGGAGCGCATGGAGCGGGAACGGCTGGAGCGCGAACGCATGCACGTGGA GCACGAGCGCAGGCGCGAGCAGGAGCGCATCCACCGTGAGCGCGAGGAGCTGAGGCGCCAGCAGGAACTG CGCTATGAGCAGGAGCGGCGGCCCGCGGTGCGGCGGCCCTACGACCTGGACCGGCGAGATGATGCCTATT GGCCGGAAGCCAAGCGGGCCGCCCTGGATGAGCGCTACCATTCTGACTTTAACCGCCAGGACCGCTTCCA CGACTTTGACCACAGGGACCGCGGCCGCTACCCCGACCACTCGGTGGACAGGAGAGAAGGTTCAAGGTCA ATGATGGGAGAACGAGAAGGACAGCATTACCCAGAACGCCATGGAGGACCAGAGCGCCACGGCCGGGACT CCCGCGATGGCTGGGGGGGCTATGGCTCTGACAAGAGGATGAGCGAGGGCCGGGGGCTGCCTCCTCCCCC CAGGGGCAGACGTGACTGGGGGGACCATGGCCGAAGAGAGGATGACCGGTCATGGCAGGGCACGGCCGAC GGGGGCATGATGGACAGGGATCACAAGAGGTGGCAAGGTGGCGAGAGAAGCATGTCCGGTCACTCCGGGC CTGGCCACATGATGAACCGAGGAGGAATGTCAGGGCGCGGCAGCTTTGCCCCAGGCGGGGCCTCCCGGGG CCACCCCATCCCACACGGTGGCATGCAGGGCGGGTTTGGAGGCCAGAGCCGGGGGAGCAGGCCCAGCGAT GCCCGCTTCACTCGCCGCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235032 representing NM_001201338 Red=Cloning site Green=Tags(s) MAETLSGLGDSGAAGAAALSSASSETGTRRLSDLRVIDLRAELRKRNVDSSGNKSVLMERLKKAIEDEGG NPDEIEITSEGNKKTSKRSSKGRKPEEEGVEDNGLEENSGDGQEDVETSLENLQDIDIMDISVLDEAEID NGSVADCVEDDDADNLQESLSDSRELVEGEMKELPEQLQEHAIEDKETINNLDTSSSDFTILQEIEEPSL EPENEKILDILGETCKSEPVKEESSELEQPFAQDTSSVGPDRKLAEEEDLFDSAHPEEGDLDLASESTAH AQSSKADSLLAVVKREPAEQPGDGERTDCEPVGLEPAVEQSSAASELAEASSEELAEAPTEAPSPEARDS KEDGRKFDFDACNEVPPAPKESSTSEGADQKMSSPEDDSDTKRLSKEEKGRSSCGRNFWVSGLSSTTRAT DLKNLFSKYGKVVGAKVVTNARSPGARCYGFVTMSTAEEATKCINHLHKTELHGKMISVEKAKNEPVGKK TSDKRDSDGKKEKSSNSDRSTNLKRDDKCDRKDDAKKGDDGSGEKSKDQDDQKPGPSERSRATKSGSRGT ERTVVMDKSKGVPVISVKTSGSKERASKSQDRKSASREKRSVVSFDKVKEPRKSRDSESHSRVRERSERE QRMQAQWEREERERLEIARERLAFQRQRLERERMERERLERERMHVEHERRREQERIHREREELRRQQEL RYEQERRPAVRRPYDLDRRDDAYWPEAKRAALDERYHSDFNRQDRFHDFDHRDRGRYPDHSVDRREGSRS MMGEREGQHYPERHGGPERHGRDSRDGWGGYGSDKRMSEGRGLPPPPRGRRDWGDHGRREDDRSWQGTAD GGMMDRDHKRWQGGERSMSGHSGPGHMMNRGGMSGRGSFAPGGASRGHPIPHGGMQGGFGGQSRGSRPSD ARFTRRY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001201338
ORF Size	2751 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001201338.2
RefSeq Size	3121 bp
RefSeq ORF	2754 bp
Locus ID	6294
UniProt ID	Q15424
Protein Families	Druggable Genome, Transcription Factors
MW	103.3 kDa
Gene Summary	This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2011]

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