RRAGC (NM_001271851) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GTR2; RAGC; TIB929
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233885 representing NM_001271851 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCTGCAGTACGGGGCGGAGGAGACGCCCCTCGCCGGCAGTTACGGCGCGGCCGATTCGTTTCCAA AGGACTTCGGCTACGGCGTGGAGGAGGAGGAAGAGGAGGCGGCGGCGGCGGGCGGAGGGGTTGGGGCAGG GGCAGGCGGTGGCTGTGGTCCGGGGGGCGCTGACAGCTCCAAGCCGAGGATTCTGCTCATGGGACTCCGG CGCAGCGGCAAGTCCTCCATCCAGAAGATATGGGATTTTCCTGGGCAAATGGACTTTTTTGACCCAACCT TTGACTATGAGATGATCTTCAGGGGAACAGGAGCATTGATATACGTCATTGACGCACAGGATGACTACAT GGAGGCTTTAACAAGACTTCACATTACTGTTTCTAAAGCCTACAAAGTTAACCCAGACATGAATTTTGAG GTTTTTATTCACAAAGTTGATGGTCTGTCTGATGATCACAAAATAGAAACACAGAGGGACATTCATCAAA GGGCCAATGATGACCTTGCAGATGCTGGGCTAGAAAAACTCCATCTTAGCTTTTATCTGACTAGTATCTA TGACCATTCAATATTTGAAGCCTTTAGTAAGGTGGTGCAGAAACTCATTCCACAACTGCCGACCTTGGAA AACCTATTAAATATCTTTATATCAAATTCAGGTATTGAAAAAGCTTTTCTCTTTGATGTTGTCAGCAAAA TCTACATTGCAACAGACAGTTCCCCTGTGGATATGCAATCTTATGAACTTTGCTGTGACATGATCGATGT TGTAATTGATGTGTCTTGTATATATGGGTTAAAGGAAGATGGAAGTGGAAGTGCTTATGACAAAGAATCT ATGGCAATTATCAAGCTGAATAATACAACTGTCCTTTATTTAAAGGAGGTGACTAAATTTTTGGCACTGG TCTGCATTCTAAGGGAAGAAAGCTTTGAAAGAAAAGGTTTAATAGACTACAACTTCCACTGTTTCCGAAA AGCTATTCATGAGGTTTTTGAGGTGGGTGTGACTTCTCACAGGAGCTGTGGTCACCAGACTAGTGCCTCC AGTCTGAAAGCGCTGACACACAATGGCACGCCACGAAACGCCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233885 representing NM_001271851 Red=Cloning site Green=Tags(s) MSLQYGAEETPLAGSYGAADSFPKDFGYGVEEEEEEAAAAGGGVGAGAGGGCGPGGADSSKPRILLMGLR RSGKSSIQKIWDFPGQMDFFDPTFDYEMIFRGTGALIYVIDAQDDYMEALTRLHITVSKAYKVNPDMNFE VFIHKVDGLSDDHKIETQRDIHQRANDDLADAGLEKLHLSFYLTSIYDHSIFEAFSKVVQKLIPQLPTLE NLLNIFISNSGIEKAFLFDVVSKIYIATDSSPVDMQSYELCCDMIDVVIDVSCIYGLKEDGSGSAYDKES MAIIKLNNTTVLYLKEVTKFLALVCILREESFERKGLIDYNFHCFRKAIHEVFEVGVTSHRSCGHQTSAS SLKALTHNGTPRNAI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271851
ORF Size	1095 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271851.2
RefSeq Size	2644 bp
RefSeq ORF	1098 bp
Locus ID	64121
UniProt ID	Q9HB90
Protein Families	Transcription Factors
MW	40.7 kDa
Gene Summary	This gene encodes a member of the GTR/RAG GTP-binding protein family. The encoded protein is a monomeric guanine nucleotide-binding protein which forms a heterodimer with RRAGA and RRAGB and is primarily localized to the cytoplasm. The encoded protein promotes intracellular localization of the mTOR complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]

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