SESN1 (NM_001199933) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PA26; SEST1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC232847 representing NM_001199933 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCTTGGCCGCCGCCGCGAACGAGGCGTACACGGCCCCTTTGGCGGTCTCGGGGCTGCTGGGCTGCA AGCAGTGCGGCGGGGGCCGCGACCAGGACGAGGAACTTGGCATTAGAATTCCTCGACCACTAGGACAGGG ACCAAGCAGATTCATCCCAGAAAAGGAGATCCTCCAAGTGGGGAGTGAAGACGCACAGATGCATGCTTTA TTTGCAGATTCTTTTGCTGCTTTGGGCCGTTTGGATAACATTACGTTAGTGATGGTTTTCCACCCACAAT ATTTAGAAAGTTTCTTAAAAACTCAGCACTATCTACTGCAAATGGATGGGCCGTTACCCCTACATTATCG TCACTACATTGGAATAATGGCTGCGGCAAGACATCAGTGCTCCTACTTAGTGAACCTGCATGTAAATGAT TTCCTTCATGTTGGTGGGGACCCCAAGTGGCTCAATGGTTTAGAGAATGCTCCTCAAAAACTACAGAATT TAGGAGAACTTAACAAAGTGTTAGCCCATAGACCTTGGCTTATTACCAAAGAACACATTGAGGGACTTTT AAAAGCTGAAGAGCACAGCTGGTCCCTTGCGGAATTGGTACATGCAGTAGTTTTACTCACACACTATCAT TCTCTTGCCTCATTCACATTCGGCTGTGGAATCAGTCCAGAAATTCATTGTGATGGTGGCCACACATTCA GACCTCCTTCTGTTAGCAACTACTGCATCTGTGACATTACAAATGGCAATCACAGTGTGGATGAGATGCC GGTCAACTCAGCAGAAAATGTTTCTGTAAGTGATTCTTTCTTTGAGGTTGAAGCCCTCATGGAAAAGATG AGGCAGTTACAGGAATGTCGAGATGAAGAAGAGGCAAGTCAGGAAGAGATGGCTTCACGTTTTGAAATAG AAAAAAGAGAGAGTATGTTTGTCTTCTCTTCAGATGATGAAGAAGTTACACCAGCAAGAGCTGTATCTCG TCATTTTGAGGATACTAGTTATGGCTATAAAGATTTCTCTAGACATGGGATGCATGTTCCAACATTTCGT GTCCAGGACTATTGCTGGGAAGATCATGGTTATTCTTTGGTAAATCGCCTTTATCCAGATGTGGGACAGT TGATTGATGAAAAATTTCACATTGCTTACAATCTTACTTATAATACAATGGCAATGCACAAAGATGTTGA TACCTCAATGCTTAGACGGGCAATTTGGAACTATATTCACTGCATGTTTGGAATAAGATATGATGATTAT GACTATGGTGAAATTAACCAGCTATTGGATCGTAGCTTTAAAGTTTATATCAAAACTGTTGTTTGCACTC CTGAAAAGGTTACCAAAAGAATGTATGATAGCTTCTGGAGGCAGTTCAAGCACTCTGAGAAGGTTCATGT TAATCTGCTTCTTATAGAAGCTAGGATGCAAGCAGAACTCCTTTATGCTCTGAGAGCCATTACCCGCTAT ATGACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC232847 representing NM_001199933 Red=Cloning site Green=Tags(s) MRLAAAANEAYTAPLAVSGLLGCKQCGGGRDQDEELGIRIPRPLGQGPSRFIPEKEILQVGSEDAQMHAL FADSFAALGRLDNITLVMVFHPQYLESFLKTQHYLLQMDGPLPLHYRHYIGIMAAARHQCSYLVNLHVND FLHVGGDPKWLNGLENAPQKLQNLGELNKVLAHRPWLITKEHIEGLLKAEEHSWSLAELVHAVVLLTHYH SLASFTFGCGISPEIHCDGGHTFRPPSVSNYCICDITNGNHSVDEMPVNSAENVSVSDSFFEVEALMEKM RQLQECRDEEEASQEEMASRFEIEKRESMFVFSSDDEEVTPARAVSRHFEDTSYGYKDFSRHGMHVPTFR VQDYCWEDHGYSLVNRLYPDVGQLIDEKFHIAYNLTYNTMAMHKDVDTSMLRRAIWNYIHCMFGIRYDDY DYGEINQLLDRSFKVYIKTVVCTPEKVTKRMYDSFWRQFKHSEKVHVNLLLIEARMQAELLYALRAITRY MT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199933
ORF Size	1476 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199933.1, NP_001186862.1
RefSeq Size	2698 bp
RefSeq ORF	1479 bp
Locus ID	27244
UniProt ID	Q9Y6P5
Protein Pathways	p53 signaling pathway
MW	57 kDa
Gene Summary	This gene encodes a member of the sestrin family. Sestrins are induced by the p53 tumor suppressor protein and play a role in the cellular response to DNA damage and oxidative stress. The encoded protein mediates p53 inhibition of cell growth by activating AMP-activated protein kinase, which results in the inhibition of the mammalian target of rapamycin protein. The encoded protein also plays a critical role in antioxidant defense by regenerating overoxidized peroxiredoxins, and the expression of this gene is a potential marker for exposure to radiation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

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