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ALOXE3 (NM_001165960) Human Tagged ORF Clone
CAT＃: RC228595
- TrueORF®
ALOXE3 (Myc-DDK-tagged)-Human arachidonate lipoxygenase 3 (ALOXE3), transcript variant 1
ORF Plasmid: tGFP
"NM_001165960" in other vectors (4)
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CNY 8,840.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARCI3; E-LOX; eLOX-3; eLOX3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC228595 representing NM_001165960
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACGCGGGGCATTCAGACCCTGTTTGCCGGCGCTGTATTTCGCTTTCCTGACCTGCCCTACTCCAG AGCAGAGAATGAGTGGAACCCAGGCTCCTGATATCCATCTGGGTGAGCCAGCCAGAGGGACCGGCTGTGT CAGAGGCAAGCAAACAAGTATTAGAGTGCAAGACTGTGGGCGGAGAGAGGAAGCCCGAGCCGCCAGCAGG GAGCTTCGGAGAGAGAAAGCCCAGGAACATCCCAGAGAGAGCTGGGCCCATCCTCAGCCCTACCCAGCCC CGCAGCCCCTAGCCCTCCGCCCAGAAACCCAGCCCTGTCCGGCGTGCCGCTCTTCTCCTCCAGGCCGGCT GCTGCTGCGGCCAGCGTTGCCGGGGCATCCCTTCCTCCTTCCCATCATGGCAGTGTACCGCCTGTGTGTG ACCACTGGTCCCTACCTGAGGGCCGGCACACTGGACAACATCTCTGTCACACTGGTGGGCACGTGTGGTG AAAGCCCCAAGCAGCGGCTAGATCGAATGGGCAGGGACTTCGCCCCTGGATCGGTACAGAAGTACAAGGT GCGTTGCACAGCGGAGCTGGGTGAGCTCTTGCTGCTGCGTGTACACAAGGAGCGCTACGCTTTCTTCCGC AAGGACTCTTGGTACTGTAGCCGCATCTGTGTCACCGAACCGGATGGTAGTGTATCCCACTTCCCCTGCT ATCAGTGGATTGAAGGCTACTGCACCGTGGAGCTGAGGCCAGGAACAGCAAGAACTATTTGTCAGGACTC TCTTCCCCTCCTCCTGGATCACAGGACACGGGAGCTCCGGGCCCGACAAGAATGCTACCGCTGGAAGATC TATGCCCCTGGCTTCCCCTGCATGGTAGACGTCAACAGCTTTCAGGAGATGGAGTCAGACAAGAAATTTG CCTTGACAAAGACGACAACTTGTGTAGACCAGGGTGACAGCAGTGGGAATCGGTACCTGCCCGGCTTCCC CATGAAAATTGACATCCCATCCCTGATGTACATGGAGCCCAATGTTCGATACTCAGCCACCAAGACGATC TCGCTGCTCTTCAATGCCATCCCTGCGTCCTTGGGAATGAAGCTTCGAGGGCTGTTGGATCGCAAGGGCT CCTGGAAGAAGCTGGATGACATGCAGAACATCTTCTGGTGCCATAAGACCTTCACGACAAAGTATGTCAC AGAGCACTGGTGTGAAGATCACTTCTTTGGGTACCAGTACCTGAATGGTGTCAATCCCGTCATGCTCCAC TGCATCTCTAGCTTGCCCAGCAAGCTGCCTGTCACCAATGACATGGTGGCCCCCTTGCTGGGACAGGACA CATGCCTGCAGACAGAGCTAGAGAGGGGGAACATCTTCCTAGCGGACTACTGGATCCTGGCGGAGGCCCC CACCCACTGCCTAAACGGCCGCCAGCAGTACGTGGCCGCCCCACTGTGCCTGCTGTGGCTCAGCCCCCAG GGGGCGCTGGTGCCCTTGGCCATCCAGCTCAGCCAGACCCCCGGGCCTGACAGCCCCATCTTCCTGCCCA CTGACTCCGAATGGGACTGGCTGCTGGCCAAGACGTGGGTGCGCAACTCTGAGTTCCTGGTGCACGAAAA CAACACGCACTTTCTGTGCACGCATTTGCTGTGCGAGGCCTTCGCCATGGCCACGCTGCGCCAGCTGCCG CTCTGCCACCCCATCTACAAGCTCCTACTCCCCCACACTCGATACACGCTGCAGGTGAACACCATCGCGA GGGCCACGCTGCTCAACCCCGAGGGCCTCGTGGACCAGGTCACGTCCATCGGGAGGCAAGGCCTCATCTA CCTCATGAGCACGGGCCTGGCCCACTTCACCTACACCAATTTCTGCCTTCCGGACAGCCTGCGGGCCCGC GGCGTCCTGGCTATCCCCAACTACCACTACCGAGACGACGGCCTGAAGATCTGGGCGGCCATTGAGAGCT TTGTCTCAGAAATCGTGGGCTACTATTATCCCAGTGACGCATCTGTGCAGCAGGATTCGGAGCTGCAGGC CTGGACTGGCGAGATTTTTGCTCAGGCGTTCCTGGGCCGGGAAAGCTCAGGTTTCCCAAGCCGGCTGTGC ACCCCAGGAGAGATGGTGAAGTTCCTCACTGCAATCATCTTCAATTGCTCTGCCCAGCACGCTGCTGTCA ACAGTGGGCAGCATGACTTTGGGGCCTGGATGCCCAATGCTCCATCATCCATGAGGCAGCCCCCACCCCA GACCAAGGGGACCACCACCCTGAAGACTTACCTAGACACCCTCCCTGAAGTGAACATCAGCTGTAACAAC CTCCTCCTCTTCTGGTTGGTTAGCCAAGAACCCAAGGACCAGAGGCCCCTGGGCACCTACCCAGATGAGC ACTTCACAGAGGAGGCCCCGAGGCGGAGCATCGCCGCCTTCCAGAGCCGCCTGGCCCAGATCTCAAGGGA CATCCAGGAGCGGAACCAGGGTCTGGCACTGCCCTACACCTACCTGGACCCTCCCCTCATTGAGAACAGC GTCTCCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC228595 representing NM_001165960
Red=Cloning site Green=Tags(s) MPRGAFRPCLPALYFAFLTCPTPEQRMSGTQAPDIHLGEPARGTGCVRGKQTSIRVQDCGRREEARAASR ELRREKAQEHPRESWAHPQPYPAPQPLALRPETQPCPACRSSPPGRLLLRPALPGHPFLLPIMAVYRLCV TTGPYLRAGTLDNISVTLVGTCGESPKQRLDRMGRDFAPGSVQKYKVRCTAELGELLLLRVHKERYAFFR KDSWYCSRICVTEPDGSVSHFPCYQWIEGYCTVELRPGTARTICQDSLPLLLDHRTRELRARQECYRWKI YAPGFPCMVDVNSFQEMESDKKFALTKTTTCVDQGDSSGNRYLPGFPMKIDIPSLMYMEPNVRYSATKTI SLLFNAIPASLGMKLRGLLDRKGSWKKLDDMQNIFWCHKTFTTKYVTEHWCEDHFFGYQYLNGVNPVMLH CISSLPSKLPVTNDMVAPLLGQDTCLQTELERGNIFLADYWILAEAPTHCLNGRQQYVAAPLCLLWLSPQ GALVPLAIQLSQTPGPDSPIFLPTDSEWDWLLAKTWVRNSEFLVHENNTHFLCTHLLCEAFAMATLRQLP LCHPIYKLLLPHTRYTLQVNTIARATLLNPEGLVDQVTSIGRQGLIYLMSTGLAHFTYTNFCLPDSLRAR GVLAIPNYHYRDDGLKIWAAIESFVSEIVGYYYPSDASVQQDSELQAWTGEIFAQAFLGRESSGFPSRLC TPGEMVKFLTAIIFNCSAQHAAVNSGQHDFGAWMPNAPSSMRQPPPQTKGTTTLKTYLDTLPEVNISCNN LLLFWLVSQEPKDQRPLGTYPDEHFTEEAPRRSIAAFQSRLAQISRDIQERNQGLALPYTYLDPPLIENS VSI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001165960 |
| ORF Size | 2529 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001165960.1, NP_001159432.1 |
| RefSeq ORF | 2532 bp |
| Locus ID | 59344 |
| UniProt ID | Q9BYJ1 |
| Protein Families | Druggable Genome |
| MW | 95 kDa |
| Gene Summary | This gene is a member of the lipoxygenase family, which are catabolized by arachidonic acid-derived compounds. The encoded enzyme is a hydroperoxide isomerase that synthesizes a unique type of epoxy alcohol (8R-hydroxy-11R,12R-epoxyeicosa-5Z,9E,14Z-trienoic acid) from 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE). This epoxy alcohol can activate the the nuclear receptor peroxisome proliferator-activated receptor alpha (PPARalpha), which is implicated in epidermal differentiation. Loss of function of the enzyme encoded by this gene results in ichthyosis, implicating the function of this gene in the differentiation of human skin. This gene is part of a cluster of lipoxygenase genes on 17p13.1. Mutations in this gene result in nonbullous congenital ichthyosiform erythroderma (NCIE). Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228595L3 | Lenti-ORF clone of ALOXE3 (Myc-DDK-tagged)-Human arachidonate lipoxygenase 3 (ALOXE3), transcript variant 1 |
CNY 10,740.00 |
|
| RC228595L4 | Lenti-ORF clone of ALOXE3 (mGFP-tagged)-Human arachidonate lipoxygenase 3 (ALOXE3), transcript variant 1 |
CNY 10,740.00 |
|
| RG228595 | ALOXE3 (tGFP-tagged) - Human arachidonate lipoxygenase 3 (ALOXE3), transcript variant 1 |
CNY 9,690.00 |
|
| SC327230 | ALOXE3 (untagged)-Human arachidonate lipoxygenase 3 (ALOXE3) transcript variant 1 |
CNY 16,340.00 |
