CGREF1 (NM_001166239) Human Tagged ORF Clone
CAT#: RC228252
- TrueORF®
CGREF1 (Myc-DDK-tagged)-Human cell growth regulator with EF-hand domain 1 (CGREF1), transcript variant 2. Note: ORF is codon optimized
ORF Plasmid: tGFP
"NM_001166239" in other vectors (2)
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CNY 2,400.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CGR11 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC228252 ORF sequence, codon optimized.
Due to the complexity of NM_001166239, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCCTCTGACCATGACCGTACTCATCCTGCTCCTTCTCCCCACCGGCCAAGCCGCCCCTAAAGACG GGGTGACTCGGCCCGATTCTGAAGTCCAGCATCAACTGCTGCCAAACCCCTTCCAACCCGGTCAGGAGCA ACTGGGTCTGCTTCAGTCCTACCTGAAGGGCCTGGGTAGAACCGAGGTGCAGCTGGAGCACCTGTCTAGG GAGCAAGTCCTTCTGTATCTGTTTGCGTTGCATGATTACGACCAGTCAGGCCAGCTGGATGGGCTTGAAC TGTTGAGTATGCTCACCGCCGCACTGGCTCCAGGGGCAGCAAACAGCCCAACCACAAACCCTGTTATTCT GATCGTTGATAAGGTGCTGGAGACACAGGACCTGAACGGTGATGGGTTGATGACTCCAGCAGAATTGATA AACTTCCCGGGTGTGGCCCTTAGACACGTTGAGCCTGGCGAACCCCTGGCACCCTCCCCACAGGAGCCCC AAGCCGTGGGACGGCAATCTCTGCTTGCTAAGTCACCCCTGAGACAGGAAACGCAGGAGGCTCCTGGCCC CAGGGAGGAAGCTAAGGGCCAAGTTGAAGCTAGGCGCGAATCACTTGACCCCGTGCAGGAGCCCGGCGGC CAGGCGGAAGCCGACGGCGATGTCCCCGGCCCGCGAGGCGAGGCTGAGGGACAGGCGGAGGCAAAAGGAG ACGCCCCAGGACCTCGCGGAGAAGCTGGGGGGCAGGCTGAGGCTGAAGGTGATGCACCAGGGCCGAGAGG CGAAGCAGGCGGACAGGCGGAAGCCGAAGGCGATGCGCCCGGGCCTCGCGGGGAGGCAGGCGGACAAGCT GAGGCCAGAGAAAATGGCGAGGAAGCGAAAGAGTTGCCTGGCGAGACTCTGGAGAGCAAAAATACCCAGA ACGATTTTGAAGTGCACATAGTACAAGTAGAAAATGATGAAATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC228252 representing NM_001166239
Red=Cloning site Green=Tags(s) MLPLTMTVLILLLLPTGQAAPKDGVTRPDSEVQHQLLPNPFQPGQEQLGLLQSYLKGLGRTEVQLEHLSR EQVLLYLFALHDYDQSGQLDGLELLSMLTAALAPGAANSPTTNPVILIVDKVLETQDLNGDGLMTPAELI NFPGVALRHVEPGEPLAPSPQEPQAVGRQSLLAKSPLRQETQEAPGPREEAKGQVEARRESLDPVQEPGG QAEADGDVPGPRGEAEGQAEAKGDAPGPRGEAGGQAEAEGDAPGPRGEAGGQAEAEGDAPGPRGEAGGQA EARENGEEAKELPGETLESKNTQNDFEVHIVQVENDEI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001166239 |
ORF Size | 954 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001166239.1, NP_001159711.1 |
RefSeq Size | 1783 bp |
RefSeq ORF | 957 bp |
Locus ID | 10669 |
UniProt ID | Q99674 |
MW | 33.5 kDa |
Gene Summary | Mediates cell-cell adhesion in a calcium-dependent manner (By similarity). Able to inhibit growth in several cell lines.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RG228252 | CGREF1 (tGFP-tagged) - Human cell growth regulator with EF-hand domain 1 (CGREF1), transcript variant 2. Note: ORF is codon optimized |
CNY 4,370.00 |
|
SC326887 | CGREF1 (untagged)-Human cell growth regulator with EF-hand domain 1 (CGREF1) transcript variant 2 |
CNY 6,270.00 |