Apoptosis inhibitor 5 (API5) (NM_001142930) Human Tagged ORF Clone
CAT#: RC227839
- TrueORF®
API5 (Myc-DDK-tagged)-Human apoptosis inhibitor 5 (API5), transcript variant 1
ORF Plasmid: tGFP
"NM_001142930" in other vectors (4)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AAC-11; AAC11 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC227839 representing NM_001142930
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGACAGTAGAGGAGCTTTACCGCAATTATGGCATCCTGGCCGATGCCACGGAGCAAGTGGGCCAGC ATAAAGATGCCTATCAAGTGATATTGGATGGTGTGAAAGGTGGTACTAAGGAAAAGCGATTAGCAGCTCA ATTTATTCCGAAATTCTTTAAGCATTTTCCAGAATTGGCTGATTCTGCTATCAATGCACAGTTAGACCTC TGTGAGGATGAAGATGTATCTATTCGACGTCAAGCAATTAAAGAACTGCCTCAATTTGCCACTGGAGAAA ATCTTCCTCGAGTGGCAGATATACTAACGCAACTTTTGCAGACAGATGACTCTGCAGAATTTAACCTAGT GAACAATGCCCTATTAAGTATATTTAAAATGGATGCAAAAGGGACTTTAGGTGGGTTGTTCAGCCAAATA CTTCAAGGAGAGGACATTGTTAGAGAACGAGCAATTAAATTCCTTTCTACAAAACTTAAGACTTTACCAG ATGAAGTCTTAACAAAGGAAGTGGAAGAGCTTATACTAACTGAATCCAAAAAGGTCCTAGAAGATGTGAC TGGTGAAGAATTTGTTCTATTTATGAAGATACTGTCTGGGTTAAAAAGCTTACAGACAGTGAGTGGAAGA CAGCAACTTGTAGAGTTGGTGGCTGAACAGGCCGACCTAGAACAGACCTTCAATCCCTCGGATCCTGACT GTGTGGACAGGCTCTTACAGTGCACTCGGCAGGCAGTACCCCTCTTCTCTAAAAATGTCCATTCCACAAG GTTTGTGACATATTTCTGTGAGCAGGTTCTCCCTAACCTCGGTACCTTGACTACCCCAGTGGAAGGTCTT GATATACAGTTGGAGGTATTGAAATTGTTGGCGGAGATGAGTTCATTTTGTGGTGACATGGAAAAACTAG AAACAAATTTAAGGAAACTATTTGATAAGTTATTGGAATACATGCCCCTCCCTCCAGAAGAGGCAGAAAA TGGAGAGAATGCTGGTAATGAAGAACCCAAGCTACAGTTCAGTTATGTGGAATGTTTGTTGTACAGTTTT CACCAGTTGGGCCGAAAACTTCCAGATTTCTTAACAGCCAAACTGAATGCAGAAAAGCTCAAAGATTTCA AAATCAGGCTGCAGTACTTTGCACGGGGCCTGCAAGTTTATATCAGACAACTTCGCTTAGCTCTCCAGGG TAAAACGGGTGAGGCCTTAAAAACAGAAGAGAACAAGATTAAAGTCGTTGCATTGAAAATAACAAACAAT ATCAATGTTTTAATCAAGGATCTCTTCCACATTCCTCCTTCTTATAAGAGCACAGTAACACTATCCTGGA AACCTGTACAAAAGGTTGAGATTGGGCAAAAGAGAGCCAGTGAAGATACAACTTCAGGTTCACCACCCAA GAAATCTTCAGCAGGACCAAAAAGAGATGCCAGGCAGATTTATAACCCTCCCAGTGGGAAATATAGCAGC AATTTGGGCAACTTTAATTATGAGCAGAGAGGAGCCTTCAGGGGAAGTAGAGGTGGCCGAGGTTGGGGCA CACGAGGAAATCGTAGTCGGGGAAGACTCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227839 representing NM_001142930
Red=Cloning site Green=Tags(s) MPTVEELYRNYGILADATEQVGQHKDAYQVILDGVKGGTKEKRLAAQFIPKFFKHFPELADSAINAQLDL CEDEDVSIRRQAIKELPQFATGENLPRVADILTQLLQTDDSAEFNLVNNALLSIFKMDAKGTLGGLFSQI LQGEDIVRERAIKFLSTKLKTLPDEVLTKEVEELILTESKKVLEDVTGEEFVLFMKILSGLKSLQTVSGR QQLVELVAEQADLEQTFNPSDPDCVDRLLQCTRQAVPLFSKNVHSTRFVTYFCEQVLPNLGTLTTPVEGL DIQLEVLKLLAEMSSFCGDMEKLETNLRKLFDKLLEYMPLPPEEAENGENAGNEEPKLQFSYVECLLYSF HQLGRKLPDFLTAKLNAEKLKDFKIRLQYFARGLQVYIRQLRLALQGKTGEALKTEENKIKVVALKITNN INVLIKDLFHIPPSYKSTVTLSWKPVQKVEIGQKRASEDTTSGSPPKKSSAGPKRDARQIYNPPSGKYSS NLGNFNYEQRGAFRGSRGGRGWGTRGNRSRGRLY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001142930 |
ORF Size | 1572 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001142930.2 |
RefSeq Size | 3782 bp |
RefSeq ORF | 1575 bp |
Locus ID | 8539 |
UniProt ID | Q9BZZ5 |
Protein Families | Druggable Genome |
MW | 59 kDa |
Gene Summary | This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC227839L3 | Lenti-ORF clone of API5 (Myc-DDK-tagged)-Human apoptosis inhibitor 5 (API5), transcript variant 1 |
CNY 5,890.00 |
|
RC227839L4 | Lenti-ORF clone of API5 (mGFP-tagged)-Human apoptosis inhibitor 5 (API5), transcript variant 1 |
CNY 5,890.00 |
|
RG227839 | API5 (tGFP-tagged) - Human apoptosis inhibitor 5 (API5), transcript variant 1 |
CNY 4,370.00 |
|
SC325162 | API5 (untagged)-Human apoptosis inhibitor 5 (API5), transcript variant 1 |
CNY 7,220.00 |
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