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LIMCH1 (NM_001112719) Human Tagged ORF Clone
CAT＃: RC226252
- TrueORF®
LIMCH1 (Myc-DDK-tagged)-Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 4
ORF Plasmid: tGFP
"NM_001112719" in other vectors (4)
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CNY 7,264.00
CNY 9,500.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LIMCH1A; LMO7B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226252 representing NM_001112719
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTCCGAAAGACAAGTCAAGGACACTGATGACATTGAAAGTCCTAAACGCAGTATCCGAGACAGTG GCTACATCGACTGCTGGGATTCCGAGCGCAGCGACTCCCTCTCTCCTCCTCGCCACGGCAGAGATGATTC CTTCGACAGCCTGGATTCCTTTGGCTCTCGCTCTCGGCAGACGCCTTCACCAGATGTAGTCCTCAGGGGA AGCAGCGATGGGAGAGGAAGCGACTCTGAATCCGACTTGCCTCATCGGAAGCTGCCAGATGTGAAGAAGG ATGACATGTCTGCACGGCGGACTTCCCATGGTGAGCCGAAATCAGCAGTGCCTTTTAACCAGTACCTCCC GAACAAAAGCAATCAGACGGCCTACGTCCCCGCGCCTCTGAGAAAGAAGAAAGCAGAGAGAGAGGAATAC CGCAAGAGCTGGAGTACCGCCACCTCCCCGCTGGGTGGGGAGAGGCCCTTCAGATACGGTCCGAGAACTC CTGTGTCTGATGACGCAGAGAGCACCAGCATGTTTGACATGCGGTGTGAGGAGGAGGCCGCGGTGCAGCC GCACAGCAGGGCCCGCCAGGAGCAGCTGCAGCTGATAAATAACCAGCTGAGGGAAGAGGACGACAAATGG CAAGATGACCTGGCTCGTTGGAAGAGTCGTAGAAGAAGTGTTTCTCAGGACTTAATCAAGAAAGAGGAAG AAAGGAAAAAAATGGAGAAGTTACTGGCTGGAGAAGATGGGACAAGTGAACGAAGGAAAAGCATCAAAAC CTACAGAGAAATTGTTCAAGAAAAAGAGCGGAGAGAGAGAGAGCTGCATGAAGCATATAAGAACGCTCGG TCCCAGGAGGAGGCAGAGGGGATCCTTCAACAGTACATTGAGAGGTTCACCATCAGTGAGGCTGTTCTCG AACGCTTGGAGATGCCAAAAATTCTGGAAAGAAGCCATTCAACAGAGCCAAATTTATCCTCCTTCCTGAA TGACCCCAATCCCATGAAATACCTGCGGCAACAGTCACTGCCTCCACCCAAATTCACTGCCACTGTTGAA ACCACCATTGCTCGTGCCAGTGTTCTGGATACCAGCATGTCAGCAGGCAGTGGGTCTCCAAGCAAAACTG TCACTCCCAAAGCAGTGCCTATGCTGACACCCAAGCCTTACTCCCAGCCCAAAAATTCTCAAGATGTTCT GAAGACCTTTAAGGTAGACGGGAAAGTCAGTGTGAATGGAGAGACGGTTCATAGAGAGGAGGAGAAGGAA AGAGAGTGTCCCACGGTGGCACCTGCCCACTCCTTAACCAAATCCCAGATGTTTGAAGGTGTGGCCAGAG TGCACGGGTCTCCACTGGAGCTGAAACAAGACAACGGTAGCATCGAGATCAACATAAAGAAGCCAAACTC TGTTCCCCAAGAGCTCGCAGCAACCACTGAGAAAACGGAACCGAATAGTCAAGAGGACAAGAATGATGGT GGAAAATCAAGAAAAGGGAATATAGAACTTGCCTCATCAGAACCACAGCATTTTACAACAACTGTGACTC GATGCAGCCCGACCGTGGCCTTTGTGGAATTTCCCTCCAGCCCCCAGCTGAAGAATGATGTGTCGGAAGA AAAAGACCAGAAGAAACCAGAAAATGAAATGAGTGGAAAGGTGGAGTTGGTGCTGTCACAAAAGGTGGTA AAGCCAAAATCTCCAGAACCCGAAGCAACGCTGACATTTCCATTTCTGGACAAAATGCCTGAAGCCAACC AACTACATTTGCCAAATCTCAATTCTCAAGTGGATTCTCCAAGCAGTGAGAAGTCACCTGTTATGACACC TTTTAAGTTCTGGGCATGGGACCCAGAAGAGGAGCGCAGGCGACAGGAAAAATGGCAACAGGAACAGGAA CGTTTGCTCCAGGAGAGATACCAGAAGGAGCAGGACAAGCTGAAAGAAGAGTGGGAAAAGGCCCAAAAGG AGGTGGAAGAGGAAGAACGCAGATACTATGAGGAGGAGCGTAAGATAATTGAAGACACTGTGGTTCCATT TACTGTTTCTTCAAGTTCCGCTGACCAGCTGTCTACCTCTTCCTCCATGACTGAAGGCAGTGGGACAATG AATAAGATAGACCTGGGAAACTGTCAAGATGAAAAACAAGACAGAAGATGGAAGAAATCATTCCAGGGAG ATGACAGTGACTTATTGCTGAAGACTAGGGAAAGTGATCGACTGGAGGAGAAGGGCAGCCTAACTGAAGG GGCCTTGGCTCATTCTGGGAACCCTGTATCAAAAGGAGTCCATGAAGACCATCAGCTGGATACCGAGGCT GGGGCCCCACACTGTGGAACAAACCCACAGCTTGCTCAGGATCCATCCCAGAATCAGCAGACATCAAATC CAACGCACAGTTCAGAAGATGTGAAGCCAAAAACCCTCCCGCTGGATAAAAGCATTAACCATCAGATCGA GTCTCCCAGTGAAAGGCGGAAGTCTATAAGTGGAAAGAAGCTGTGCTCTTCCTGTGGGCTTCCTTTGGGT AAAGGAGCTGCAATGATCATCGAGACCCTCAATCTCTATTTTCACATCCAGTGTTTCAGGTGTGGAATTT GTAAAGGCCAGCTTGGAGATGCAGTGAGTGGGACGGATGTTAGGATTCGAAATGGTCTCCTGAACTGTAA TGATTGCTACATGCGATCCAGAAGTGCCGGGCAGCCTACAACATTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226252 representing NM_001112719
Red=Cloning site Green=Tags(s) MDSERQVKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSRQTPSPDVVLRG SSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYVPAPLRKKKAEREEY RKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQLQLINNQLREEDDKW QDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNAR SQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVE TTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKE RECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDG GKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVV KPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQE RLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTM NKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEA GAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLG KGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001112719 |
| ORF Size | 2706 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001112719.3 |
| RefSeq ORF | 2709 bp |
| Locus ID | 22998 |
| UniProt ID | Q9UPQ0 |
| MW | 101.8 kDa |
| Gene Summary | Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226252L3 | Lenti-ORF clone of LIMCH1 (Myc-DDK-tagged)-Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 4 |
CNY 11,400.00 |
|
| RC226252L4 | Lenti-ORF clone of LIMCH1 (mGFP-tagged)-Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 4 |
CNY 9,664.00 |
|
| RG226252 | LIMCH1 (tGFP-tagged) - Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 4 |
CNY 10,450.00 |
|
| SC319002 | LIMCH1 (untagged)-Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 4 |
CNY 17,390.00 |
