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LIMCH1 (NM_001112719) Human Untagged Clone
CAT＃: SC319002
LIMCH1 (untagged)-Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 4
CNY 17,390.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LIMCH1A; LMO7B |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_001112719, the custom clone sequence may differ by one or more nucleotides
ATGGATTCCGAAAGACAAGTCAAGGACACTGATGACATTGAAAGTCCTAAACGCAGTATC CGAGACAGTGGCTACATCGACTGCTGGGATTCCGAGCGCAGCGACTCCCTCTCTCCTCCT CGCCACGGCAGAGATGATTCCTTCGACAGCCTGGATTCCTTTGGCTCTCGCTCTCGGCAG ACGCCTTCACCAGATGTAGTCCTCAGGGGAAGCAGCGATGGGAGAGGAAGCGACTCTGAA TCCGACTTGCCTCATCGGAAGCTGCCAGATGTGAAGAAGGATGACATGTCTGCACGGCGG ACTTCCCATGGTGAGCCGAAATCAGCAGTGCCTTTTAACCAGTACCTCCCGAACAAAAGC AATCAGACGGCCTACGTCCCCGCGCCTCTGAGAAAGAAGAAAGCAGAGAGAGAGGAATAC CGCAAGAGCTGGAGTACCGCCACCTCCCCGCTGGGTGGGGAGAGGCCCTTCAGATACGGT CCGAGAACTCCTGTGTCTGATGACGCAGAGAGCACCAGCATGTTTGACATGCGGTGTGAG GAGGAGGCCGCGGTGCAGCCGCACAGCAGGGCCCGCCAGGAGCAGCTGCAGCTGATAAAT AACCAGCTGAGGGAAGAGGACGACAAATGGCAAGATGACCTGGCTCGTTGGAAGAGTCGT AGAAGAAGTGTTTCTCAGGACTTAATCAAGAAAGAGGAAGAAAGGAAAAAAATGGAGAAG TTACTGGCTGGAGAAGATGGGACAAGTGAACGAAGGAAAAGCATCAAAACCTACAGAGAA ATTGTTCAAGAAAAAGAGCGGAGAGAGAGAGAGCTGCATGAAGCATATAAGAACGCTCGG TCCCAGGAGGAGGCAGAGGGGATCCTTCAACAGTACATTGAGAGGTTCACCATCAGTGAG GCTGTTCTCGAACGCTTGGAGATGCCAAAAATTCTGGAAAGAAGCCATTCAACAGAGCCA AATTTATCCTCCTTCCTGAATGACCCCAATCCCATGAAATACCTGCGGCAACAGTCACTG CCTCCACCCAAATTCACTGCCACTGTTGAAACCACCATTGCTCGTGCCAGTGTTCTGGAT ACCAGCATGTCAGCAGGCAGTGGGTCTCCAAGCAAAACTGTCACTCCCAAAGCAGTGCCT ATGCTGACACCCAAGCCTTACTCCCAGCCCAAAAATTCTCAAGATGTTCTGAAGACCTTT AAGGTAGACGGGAAAGTCAGTGTGAATGGAGAGACGGTTCATAGAGAGGAGGAGAAGGAA AGAGAGTGTCCCACGGTGGCACCTGCCCACTCCTTAACCAAATCCCAGATGTTTGAAGGT GTGGCCAGAGTGCACGGGTCTCCACTGGAGCTGAAACAAGACAACGGTAGCATCGAGATC AACATAAAGAAGCCAAACTCTGTTCCCCAAGAGCTCGCAGCAACCACTGAGAAAACGGAA CCGAATAGTCAAGAGGACAAGAATGATGGTGGAAAATCAAGAAAAGGGAATATAGAACTT GCCTCATCAGAACCACAGCATTTTACAACAACTGTGACTCGATGCAGCCCGACCGTGGCC TTTGTGGAATTTCCCTCCAGCCCCCAGCTGAAGAATGATGTGTCGGAAGAAAAAGACCAG AAGAAACCAGAAAATGAAATGAGTGGAAAGGTGGAGTTGGTGCTGTCACAAAAGGTGGTA AAGCCAAAATCTCCAGAACCCGAAGCAACGCTGACATTTCCATTTCTGGACAAAATGCCT GAAGCCAACCAACTACATTTGCCAAATCTCAATTCTCAAGTGGATTCTCCAAGCAGTGAG AAGTCACCTGTTATGACACCTTTTAAGTTCTGGGCATGGGACCCAGAAGAGGAGCGCAGG CGACAGGAAAAATGGCAACAGGAACAGGAACGTTTGCTCCAGGAGAGATACCAGAAGGAG CAGGACAAGCTGAAAGAAGAGTGGGAAAAGGCCCAAAAGGAGGTGGAAGAGGAAGAACGC AGATACTATGAGGAGGAGCGTAAGATAATTGAAGACACTGTGGTTCCATTTACTGTTTCT TCAAGTTCCGCTGACCAGCTGTCTACCTCTTCCTCCATGACTGAAGGCAGTGGGACAATG AATAAGATAGACCTGGGAAACTGTCAAGATGAAAAACAAGACAGAAGATGGAAGAAATCA TTCCAGGGAGATGACAGTGACTTATTGCTGAAGACTAGGGAAAGTGATCGACTGGAGGAG AAGGGCAGCCTAACTGAAGGGGCCTTGGCTCATTCTGGGAACCCTGTATCAAAAGGAGTC CATGAAGACCATCAGCTGGATACCGAGGCTGGGGCCCCACACTGTGGAACAAACCCACAG CTTGCTCAGGATCCATCCCAGAATCAGCAGACATCAAATCCAACGCACAGTTCAGAAGAT GTGAAGCCAAAAACCCTCCCGCTGGATAAAAGCATTAACCATCAGATCGAGTCTCCCAGT GAAAGGCGGAAGTCTATAAGTGGAAAGAAGCTGTGCTCTTCCTGTGGGCTTCCTTTGGGT AAAGGAGCTGCAATGATCATCGAGACCCTCAATCTCTATTTTCACATCCAGTGTTTCAGG TGTGGAATTTGTAAAGGCCAGCTTGGAGATGCAGTGAGTGGGACGGATGTTAGGATTCGA AATGGTCTCCTGAACTGTAATGATTGCTACATGCGATCCAGAAGTGCCGGGCAGCCTACA ACATTG |
| Restriction Sites | Please inquire |
| ACCN | NM_001112719 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001112719.1, NP_001106190.1 |
| RefSeq Size | 5758 bp |
| RefSeq ORF | 2709 bp |
| Locus ID | 22998 |
| UniProt ID | Q9UPQ0 |
| Gene Summary | Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR and coding region and uses an alternate in-frame splice site compared to variant 1. The resulting isoform (d) has a shorter and distinct N-terminus compared to isoform a. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226252 | LIMCH1 (Myc-DDK-tagged)-Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 4 |
CNY 7,264.00 |
|
| RC226252L3 | Lenti-ORF clone of LIMCH1 (Myc-DDK-tagged)-Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 4 |
CNY 11,400.00 |
|
| RC226252L4 | Lenti-ORF clone of LIMCH1 (mGFP-tagged)-Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 4 |
CNY 9,664.00 |
|
| RG226252 | LIMCH1 (tGFP-tagged) - Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 4 |
CNY 10,450.00 |
