CCRL2 (NM_001130910) Human Tagged ORF Clone

CAT#: RC225528

CCRL2 (Myc-DDK-tagged)-Human chemokine (C-C motif) receptor-like 2 (CCRL2), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_001130910" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-CCRL2 Antibody
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms ACKR5; CKRX; CRAM; CRAM-A; CRAM-B; HCR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC225528 representing NM_001130910
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCTACACCCGTTTCTTAAAAGGCAGTCTGAAGATGGCCAATTACACGCTGGCACCAGAGGATGAAT
ATGATGTCCTCATAGAAGGTGAACTGGAGAGCGATGAGGCAGAGCAATGTGACAAGTATGACGCCCAGGC
ACTCTCAGCCCAGCTGGTGCCATCACTCTGCTCTGCTGTGTTTGTGATCGGTGTCCTGGACAATCTCCTG
GTTGTGCTTATCCTGGTAAAATATAAAGGACTCAAACGCGTGGAAAATATCTATCTTCTAAACTTGGCAG
TTTCTAACTTGTGTTTCTTGCTTACCCTGCCCTTCTGGGCTCATGCTGGGGGCGATCCCATGTGTAAAAT
TCTCATTGGACTGTACTTCGTGGGCCTGTACAGTGAGACATTTTTCAATTGCCTTCTGACTGTGCAAAGG
TACCTAGTGTTTTTGCACAAGGGAAACTTTTTCTCAGCCAGGAGGAGGGTGCCCTGTGGCATCATTACAA
GTGTCCTGGCATGGGTAACAGCCATTCTGGCCACTTTGCCTGAATTCGTGGTTTATAAACCTCAGATGGA
AGACCAGAAATACAAGTGTGCATTTAGCAGAACTCCCTTCCTGCCAGCTGATGAGACATTCTGGAAGCAT
TTTCTGACTTTAAAAATGAACATTTCGGTTCTTGTCCTCCCCCTATTTATTTTTACATTTCTCTATGTGC
AAATGAGAAAAACACTAAGGTTCAGGGAGCAGAGGTATAGCCTTTTCAAGCTTGTTTTTGCCATAATGGT
AGTCTTCCTTCTGATGTGGGCGCCCTACAATATTGCATTTTTCCTGTCCACTTTCAAAGAACACTTCTCC
CTGAGTGACTGCAAGAGCAGCTACAATCTGGACAAAAGTGTTCACATCACTAAACTCATCGCCACCACCC
ACTGCTGCATCAACCCTCTCCTGTATGCGTTTCTTGATGGGACATTTAGCAAATACCTCTGCCGCTGTTT
CCATCTGCGTAGTAACACCCCACTTCAACCCAGGGGGCAGTCTGCACAAGGCACATCGAGGGAAGAACCT
GACCATTCCACCGAAGTG


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>RC225528 representing NM_001130910
Red=Cloning site Green=Tags(s)

MIYTRFLKGSLKMANYTLAPEDEYDVLIEGELESDEAEQCDKYDAQALSAQLVPSLCSAVFVIGVLDNLL
VVLILVKYKGLKRVENIYLLNLAVSNLCFLLTLPFWAHAGGDPMCKILIGLYFVGLYSETFFNCLLTVQR
YLVFLHKGNFFSARRRVPCGIITSVLAWVTAILATLPEFVVYKPQMEDQKYKCAFSRTPFLPADETFWKH
FLTLKMNISVLVLPLFIFTFLYVQMRKTLRFREQRYSLFKLVFAIMVVFLLMWAPYNIAFFLSTFKEHFS
LSDCKSSYNLDKSVHITKLIATTHCCINPLLYAFLDGTFSKYLCRCFHLRSNTPLQPRGQSAQGTSREEP
DHSTEV

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001130910
ORF Size 1068 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001130910.2
RefSeq ORF 1071 bp
Locus ID 9034
UniProt ID O00421
Protein Families Druggable Genome, GPCR, Transmembrane
MW 40.8 kDa
Gene Summary This gene encodes a chemokine receptor like protein, which is predicted to be a seven transmembrane protein and most closely related to CCR1. Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. This gene is expressed at high levels in primary neutrophils and primary monocytes, and is further upregulated on neutrophil activation and during monocyte to macrophage differentiation. The function of this gene is unknown. This gene is mapped to the region where the chemokine receptor gene cluster is located. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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