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DDX19B (NM_001014451) Human Tagged ORF Clone
CAT＃: RC224541
DDX19B (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-As) box polypeptide 19B (DDX19B), transcript variant 2
ORF Plasmid: tGFP
"NM_001014451" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DBP5; DDX19; RNAh |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224541 representing NM_001014451
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACTGACTCATGGGCCCTGGCGGTGGACGAGCAGGAAGCTGCGGCTGAGTCGTTGAGCAACTTGC ATCTTAAGGAAGAGAAAATCAAACCAGATACCAATGGTGCTGTTGTCAAGACCAATGCCAATGCAGAGAA GACAGATGAAGAAGAGAAAGAGGACAGAGCTGCCCAGTCCTTACTCAACAAGCTGATCAGAAGCAACCTT GTTGATAACACAAACCAAGTGGAAGTCCTGCAGCGGGATCCAAACTCCCCTCTGTACTCGGTGAAGTCTT TTGAAGAGCTTCGGCTCCCACAGAACTTAATTGCCCAATCTCAGTCTGGTACTGGTAAAACAGCTGCCTT CGTGCTGGCCATGCTTAGCCAAGTAGAACCTGCAAACAAATACCCCCAGTGTCTATGTCTCTCCCCAACG TATGAGCTCGCCCTCCAAACAGGAAAAGTGATTGAACAAATGGGCAAATTTTACCCTGAACTGAAGCTAG CTTATGCTGTTCGAGGCAATAAATTGGAAAGAGGCCAGAAGATCAGTGAGCAGATTGTCATTGGCACCCC TGGGACTGTGCTGGACTGGTGCTCCAAGCTCAAGTTCATTGATCCCAAGAAAATCAAGGTGTTTGTTCTG GATGAGGCTGATGTCATGATAGCCACTCAGGGCCACCAAGATCAGAGCATCCGCATCCAGAGGATGCTGC CCAGGAACTGCCAGATGCTGCTTTTCTCCGCCACCTTTGAAGACTCTGTGTGGAAGTTTGCCCAGAAAGT GGTCCCAGACCCAAACGTTATCAAACTGAAGCGTGAGGAAGAGACCCTGGACACCATCAAGCAGTACTAT GTCCTGTGCAGCAGCAGAGACGAGAAGTTCCAGGCCTTGTGTAACCTCTACGGGGCCATCACCATTGCTC AAGCCATGATCTTCTGCCATACTCGCAAAACAGCTAGTTGGCTGGCAGCAGAGCTCTCAAAAGAAGGCCA CCAGGTGGCTCTGCTGAGTGGGGAGATGATGGTGGAACAGAGGGCTGCAGTGATTGAGCGCTTCCGAGAG GGCAAAGAGAAGGTTTTGGTGACCACCAACGTGTGTGCCCGCGGCATTGATGTTGAACAAGTGTCTGTCG TCATCAACTTTGATCTTCCCGTGGACAAGGACGGGAATCCTGACAATGAGACCTACCTGCACCGGATCGG GCGCACGGGCCGCTTTGGCAAGAGGGGCCTGGCAGTGAACATGGTGGACAGCAAGCACAGCATGAACATC CTGAACAGAATCCAGGAGCATTTTAATAAGAAGATAGAAAGATTGGACACAGATGATTTGGACGAGATTG AGAAAATAGCCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224541 representing NM_001014451
Red=Cloning site Green=Tags(s) MATDSWALAVDEQEAAAESLSNLHLKEEKIKPDTNGAVVKTNANAEKTDEEEKEDRAAQSLLNKLIRSNL VDNTNQVEVLQRDPNSPLYSVKSFEELRLPQNLIAQSQSGTGKTAAFVLAMLSQVEPANKYPQCLCLSPT YELALQTGKVIEQMGKFYPELKLAYAVRGNKLERGQKISEQIVIGTPGTVLDWCSKLKFIDPKKIKVFVL DEADVMIATQGHQDQSIRIQRMLPRNCQMLLFSATFEDSVWKFAQKVVPDPNVIKLKREEETLDTIKQYY VLCSSRDEKFQALCNLYGAITIAQAMIFCHTRKTASWLAAELSKEGHQVALLSGEMMVEQRAAVIERFRE GKEKVLVTTNVCARGIDVEQVSVVINFDLPVDKDGNPDNETYLHRIGRTGRFGKRGLAVNMVDSKHSMNI LNRIQEHFNKKIERLDTDDLDEIEKIAN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001014451 |
| ORF Size | 1344 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001014451.3 |
| RefSeq Size | 1738 bp |
| RefSeq ORF | 1347 bp |
| Locus ID | 11269 |
| UniProt ID | Q9UMR2 |
| MW | 50.3 kDa |
| Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which exhibits RNA-dependent ATPase and ATP-dependent RNA-unwinding activities. This protein is recruited to the cytoplasmic fibrils of the nuclear pore complex, where it participates in the export of mRNA from the nucleus. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224541L3 | Lenti-ORF clone of DDX19B (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-As) box polypeptide 19B (DDX19B), transcript variant 2 |
CNY 5,890.00 |
|
| RC224541L4 | Lenti-ORF clone of DDX19B (mGFP-tagged)-Human DEAD (Asp-Glu-Ala-As) box polypeptide 19B (DDX19B), transcript variant 2 |
CNY 5,890.00 |
|
| RG224541 | DDX19B (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-As) box polypeptide 19B (DDX19B), transcript variant 2 |
CNY 4,370.00 |
|
| SC301949 | DDX19B (untagged)-Human DEAD (Asp-Glu-Ala-As) box polypeptide 19B (DDX19B), transcript variant 2 |
CNY 7,130.00 |
