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DDX19B (NM_001014451) Human Untagged Clone
CAT＃: SC301949
DDX19B (untagged)-Human DEAD (Asp-Glu-Ala-As) box polypeptide 19B (DDX19B), transcript variant 2
CNY 7,130.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DBP5; DDX19; RNAh |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC301949 representing NM_001014451.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCACTGACTCATGGGCCCTGGCGGTGGACGAGCAGGAAGCTGCGGCTGAGTCGTTGAGCAACTTG CATCTTAAGGAAGAGAAAATCAAACCAGATACCAATGGTGCTGTTGTCAAGACCAATGCCAATGCAGAG AAGACAGATGAAGAAGAGAAAGAGGACAGAGCTGCCCAGTCCTTACTCAACAAGCTGATCAGAAGCAAC CTTGTTGATAACACAAACCAAGTGGAAGTCCTGCAGCGGGATCCAAACTCCCCTCTGTACTCGGTGAAG TCTTTTGAAGAGCTTCGGCTCCCACAGAACTTAATTGCCCAATCTCAGTCTGGTACTGGTAAAACAGCT GCCTTCGTGCTGGCCATGCTTAGCCAAGTAGAACCTGCAAACAAATACCCCCAGTGTCTATGTCTCTCC CCAACGTATGAGCTCGCCCTCCAAACAGGAAAAGTGATTGAACAAATGGGCAAATTTTACCCTGAACTG AAGCTAGCTTATGCTGTTCGAGGCAATAAATTGGAAAGAGGCCAGAAGATCAGTGAGCAGATTGTCATT GGCACCCCTGGGACTGTGCTGGACTGGTGCTCCAAGCTCAAGTTCATTGATCCCAAGAAAATCAAGGTG TTTGTTCTGGATGAGGCTGATGTCATGATAGCCACTCAGGGCCACCAAGATCAGAGCATCCGCATCCAG AGGATGCTGCCCAGGAACTGCCAGATGCTGCTTTTCTCCGCCACCTTTGAAGACTCTGTGTGGAAGTTT GCCCAGAAAGTGGTCCCAGACCCAAACGTTATCAAACTGAAGCGTGAGGAAGAGACCCTGGACACCATC AAGCAGTACTATGTCCTGTGCAGCAGCAGAGACGAGAAGTTCCAGGCCTTGTGTAACCTCTACGGGGCC ATCACCATTGCTCAAGCCATGATCTTCTGCCATACTCGCAAAACAGCTAGTTGGCTGGCAGCAGAGCTC TCAAAAGAAGGCCACCAGGTGGCTCTGCTGAGTGGGGAGATGATGGTGGAACAGAGGGCTGCAGTGATT GAGCGCTTCCGAGAGGGCAAAGAGAAGGTTTTGGTGACCACCAACGTGTGTGCCCGCGGCATTGATGTT GAACAAGTGTCTGTCGTCATCAACTTTGATCTTCCCGTGGACAAGGACGGGAATCCTGACAATGAGACC TACCTGCACCGGATCGGGCGCACGGGCCGCTTTGGCAAGAGGGGCCTGGCAGTGAACATGGTGGACAGC AAGCACAGCATGAACATCCTGAACAGAATCCAGGAGCATTTTAATAAGAAGATAGAAAGATTGGACACA GATGATTTGGACGAGATTGAGAAAATAGCCAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001014451 |
| Insert Size | 1347 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001014451.2 |
| RefSeq Size | 1736 bp |
| RefSeq ORF | 1347 bp |
| Locus ID | 11269 |
| UniProt ID | Q9UMR2 |
| MW | 50.5 kDa |
| Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which exhibits RNA-dependent ATPase and ATP-dependent RNA-unwinding activities. This protein is recruited to the cytoplasmic fibrils of the nuclear pore complex, where it participates in the export of mRNA from the nucleus. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, as compared to variant 1. The encoded isoform (2) lacks an internal segment, as compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224541 | DDX19B (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-As) box polypeptide 19B (DDX19B), transcript variant 2 |
CNY 3,656.00 |
|
| RC224541L3 | Lenti-ORF clone of DDX19B (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-As) box polypeptide 19B (DDX19B), transcript variant 2 |
CNY 5,890.00 |
|
| RC224541L4 | Lenti-ORF clone of DDX19B (mGFP-tagged)-Human DEAD (Asp-Glu-Ala-As) box polypeptide 19B (DDX19B), transcript variant 2 |
CNY 5,890.00 |
|
| RG224541 | DDX19B (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-As) box polypeptide 19B (DDX19B), transcript variant 2 |
CNY 4,370.00 |
