Baf180 (PBRM1) (NM_018313) Human Tagged ORF Clone
CAT#: RC223997
- TrueORF®
PBRM1 (Myc-DDK-tagged)-Human polybromo 1 (PBRM1), transcript variant 2
ORF Plasmid: tGFP
"NM_018313" in other vectors (4)
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CNY 10,824.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BAF180; PB1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC223997 representing NM_018313
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTTCCAAGAGAAGAAGAGCTACCTCCCCTTCCAGCAGTGTCAGCGGGGACTTTGATGATGGGCACC ATTCTGTGTCAACACCAGGCCCAAGCAGGAAAAGGAGGAGACTTTCCAATCTTCCAACTGTAGATCCTAT TGCCGTGTGCCATGAACTCTATAATACCATCCGAGACTATAAGGATGAACAGGGCAGACTTCTCTGTGAG CTCTTCATTAGGGCACCAAAGCGAAGAAATCAACCAGACTATTATGAAGTGGTTTCTCAGCCCATTGACT TGATGAAAATCCAACAGAAACTAAAAATGGAAGAGTATGATGATGTTAATTTGCTGACTGCTGACTTCCA GCTTCTTTTTAACAATGCAAAGTCCTATTATAAGCCAGATTCTCCTGAATATAAAGCCGCTTGCAAACTC TGGGATTTGTACCTTCGAACAAGAAATGAGTTTGTTCAGAAAGGAGAAGCAGATGACGAAGATGATGATG AAGATGGGCAAGACAATCAGGGCACAGTGACTGAAGGATCTTCTCCAGCTTACTTGAAGGAGATCCTGGA GCAGCTTCTTGAAGCCATAGTTGTAGCTACAAATCCATCAGGACGTCTCATTAGCGAACTTTTTCAGAAA CTGCCTTCTAAAGTGCAATATCCAGATTATTATGCAATAATTAAGGAGCCTATAGATCTCAAGACCATTG CCCAGAGGATACAGAATGGAAGCTACAAAAGTATTCATGCAATGGCCAAAGATATAGATCTCCTCGCAAA AAATGCCAAAACTTATAATGAGCCTGGCTCTCAAGTATTCAAGGATGCAAATTCAATTAAAAAAATATTY TATATGAAAAAGGCTGAAATTGAACATCATGAAATGGCTAAGTCAAGTCTTCGAATGAGGACTCCATCCA ACCTTGCTGCAGCCAGACTGACAGGTCCTTCACACAGTAAAGGCAGCCTTGGTGAAGAGAGAAATCCCAC TAGCAAGTATTACCGTAATAAAAGAGCAGTACAAGGAGGTCGTTTATCAGCAATTACAATGGCACTTCAA TATGGCTCAGAAAGTGAAGAAGATGCTGCTTTAGCTGCTGCACGCTATGAAGAGGGAGAGTCAGAAGCAG AAAGCATCACTTCCTTTATGGATGTTTCAAATCCTTTTTATCAGCTTTATGACACAGTTAGGAGTTGTCG GAATAACCAAGGGCAGCTAATAGCTGAACCTTTTTACCATTTGCCTTCAAAGAAAAAATACCCTGATTAT TACCAGCAAATTAAAATGCCCATATCACTACAACAGATCCGAACAAAACTGAAGAATCAAGAATATGAAA CTTTAGATCATTTGGAGTGTGATCTGAATTTAATGTTTGAAAATGCCAAACGCTATAATGTGCCCAATTC AGCCATCTACAAGCGAGTTCTAAAATTGCAGCAAGTTATGCAGGCAAAGAAGAAAGAGCTTGCCAGGAGA GACGATATCGAGGACGGAGACAGCATGATCTCTTCAGCCACCTCTGATACTGGTAGTGCCAAAAGAAAAA GTAAAAAGAACATAAGAAAGCAGCGAATGAAAATCTTATTCAATGTTGTTCTTGAAGCTCGAGAGCCAGG TTCAGGCAGAAGACTTTGTGACCTATTTATGGTTAAACCATCCAAAAGGGACTATCCTGATTATTATAAA ATCATCTTGGAGCCAATGGACTTGAAAATAATTGAGCATAACATCCGCAATGACAAATATGCTGGTGAAG AGGGAATGATAGAAGACATGAAGCTGATGTTCCGGAATGCCAGGCACTATAATGAGGAGGGCTCCCAGGT TTATAATGATGCACATATCCTGGAGAAGTTACTCAAGGAGAAAAGGAAAGAGCTGGGCCCACTGCCTGAT GATGATGACATGGCTTCTCCCAAACTCAAGCTGAGTAGGAAGAGTGGCATTTCTCCTAAAAAATCAAAAT ACATGACTCCAATGCAGCAGAAACTAAATGAGGTCTATGAAGCTGTAAAGAACTATACTGATAAGAGGGG TCGCCGCCTCAGTGCCATATTTCTGAGGCTTCCCTCTAGATCTGAGTTGCCTGACTACTATCTGACTATT AAAAAGCCCATGGACATGGAAAAAATTCGAAGTCACATGATGGCCAACAAGTACCAAGATATTGACTCTA TGGTTGAGGACTTTGTCATGATGTTTAATAATGCCTGTACCTACAATGAACCGGAGTCTTTGATCTACAA AGATGCTCTTGTTCTACACAAAGTCCTGCTTGAAACACGCAGAGACCTGGAGGGAGATGAGGACTCTCAT GTCCCAAATGTGACTTTGCTGATTCAAGAGCTTATCCACAATCTTTTTGTGTCAGTCATGAGTCATCAGG ATGATGAGGGAAGATGCTACAGCGATTCTTTAGCAGAAATTCCTGCTGTGGATCCCAACTTTCCTAACAA ACCACCCCTTACATTTGACATAATTAGGAAGAATGTTGAAAATAATCGCTACCGTCGGCTTGATTTATTT CAAGAGCATATGTTTGAAGTATTGGAACGAGCAAGAAGGATGAATCGGACAGATTCAGAAATATATGAAG ATGCAGTAGAACTTCAGCAGTTTTTTATTAAAATTCGTGATGAACTCTGCAAAAATGGAGAGATTCTTCT TTCACCGGCACTCAGCTATACCACAAAACATTTGCATAATGATGTGGAGAAAGAGAGAAAGGAAAAATTG CCAAAAGAAATAGAGGAAGATAAACTAAAACGAGAAGAAGAAAAAAGAGAAGCTGAAAAGAGTGAAGATT CCTCTGGTGCTGCAGGCCTCTCAGGCTTACATCGCACATACAGCCAGGACTGTAGCTTTAAAAACAGCAT GTACCATGTTGGAGATTACGTCTATGTGGAACCTGCAGAGGCCAACCTACAACCACATATCGTCTGTATT GAAAGACTGTGGGAGGATTCAGCTGAAAAAGAAGTTTTTAAGAGTGACTATTACAACAAAGTTCCAGTTA GTAAAATTCTAGGCAAGTGTGTGGTCATGTTTGTCAAGGAATACTTTAAGTTATGCCCAGAAAACTTCCG AGATGAGGATGTTTTTGTCTGTGAATCACGGTATTCTGCCAAAACCAAATCTTTTAAGAAAATTAAACTG TGGACCATGCCCATCAGCTCAGTCAGGTTTGTCCCTCGGGATGTGCCTCTGCCTGTGGTTCGCGTGGCCT CTGTATTTGCAAATGCAGATAAAGGTGATGATGAGAAGAATACAGACAACTCAGAGGACAGTCGAGCTGA AGACAATTTTAACTTGGAAAAGGAAAAAGAAGATGTCCCTGTGGAAATGTCCAATGGTGAACCAGTTTGC CACTACTTTGAGCAGCTCCATTACAATGACATGTGGCTGAAGGTTGGCGACTGTGTCTTCATCAAGTCCC ATGGCCTGGTGCGTCCTCGTGTGGGCAGAATTGAAAAAGTATGGGTTCGAGATGGAGCTGCATATTTTTA TGGCCCCATCTTCATTCACCCAGAAGAAACAGAGCATGAGCCCACAAAAATGTTCTACAAAAAAGAAGTA TTTCTGAGTAATCTGGAAGAAACCTGCCCCATGACATGTATTCTCGGAAAGTGTGCTGTGTTGTCATTCA AGGACTTCCTCTCCTGCAAGCCAACTGAAATACCAGAAAATGACATTCTGCTTTGTGAGAGCCGCTACAA TGAGAGCGACAAGCAGATGAAGAAATTCAAAGGATTGAAGAGGTTTTCACTCTCTGCTAAAGTGGTAGAT GATGAAATTTACTACTTCAGAAAACCAATTGTTCCTCAGAAGGAGCCATCACCTTTGCTGGGAAAGAAGA TTCAGTTGCTAGAAGCTAAATTTGCCGAGTTAGAAGGTGGAGATGATGATATTGAAGAGATGGGAGAAGA AGATAGTGAGTCTACCCCAAAGTCTGCCAAAGGCAGTGCAAAGAAGGAAGGCTCCAAACGGAAAATCAAC ATGAGTGGCTACATCCTGTTCAGCAGTGAGATGAGGGCTGTGATTAAGGCCCAACACCCAGACTACTCTT TCGGGGAGCTCAGCCGCCTGGTGGGGACAGAATGGAGAAATCTTGAGACAGCCAAGAAAGCAGAATATGA AGGCATGATGGGTGGCTATCCGCCAGGCCTTCCACCTTTGCAGGGCCCAGTTGATGGCCTTGTTAGCATG GGCAGCATGCAGCCACTTCACCCTGGGGGGCCTCCACCCCACCATCTTCCGCCAGGTGTGCCTGGCCTCC CGGGCATCCCACCACCGGGTGTGATGAACCAAGGAGTGGCCCCTATGGTAGGGACTCCAGCACCGGGTGG AAGTCCATATGGACAACAGGTGGGAGTTTTGGGGCCTCCAAGGCAGCAGGCACCACCTCCATATCCCGGC CCACATCCAGCTGGACCCCCTGTCATACAGCAGCCAACAACACCCATGTTTGTAGCTCCCCCGCCAAAGA CCCAGCGGCTTCTTCACTCAGAGGCCTACCTGAAATACATTGAAGGACTCAGTGCGGAGTCCAACAGCAT TAGCAAGTGGGATCAGACACTGGCAGCTCGAAGACGCGACGTCCATTTGTCGAAAGAACAGGAGAGCCGC CTACCCTCTCACTGGCTGAAAAGCAAAGGGGCCCACACCACCATGGCAGATGCCCTCTGGCGCCTTCGAG ATTTGATGCTCCGGGACACCCTCAACATTCGCCAAGCATACAACCTAGAAAATGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223997 representing NM_018313
Red=Cloning site Green=Tags(s) MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCE LFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKSYYKPDSPEYKAACKL WDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPAYLKEILEQLLEAIVVATNPSGRLISELFQK LPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIX YMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQ YGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFYHLPSKKKYPDY YQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARR DDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKRDYPDYYK IILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPD DDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTI KKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSH VPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLF QEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKERKEKL PKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCI ERLWEDSAEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKL WTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPVC HYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEV FLSNLEETCPMTCILGKCAVLSFKDFLSCKPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVD DEIYYFRKPIVPQKEPSPLLGKKIQLLEAKFAELEGGDDDIEEMGEEDSESTPKSAKGSAKKEGSKRKIN MSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGMMGGYPPGLPPLQGPVDGLVSM GSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPRQQAPPPYPG PHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESR LPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_018313 |
ORF Size | 4746 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018313.2, NP_060783.2 |
RefSeq Size | 5070 bp |
RefSeq ORF | 4749 bp |
Locus ID | 55193 |
UniProt ID | Q86U86 |
Domains | BAH |
Protein Families | Druggable Genome |
MW | 180.9 kDa |
Gene Summary | This locus encodes a subunit of ATP-dependent chromatin-remodeling complexes. The encoded protein has been identified as in integral component of complexes necessary for ligand-dependent transcriptional activation by nuclear hormone receptors. Mutations at this locus have been associated with primary clear cell renal cell carcinoma. [provided by RefSeq, Feb 2012] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Functional role of SETD2, BAP1, PARP-3 and PBRM1 candidate genes on the regulation of hTERT gene expression
,null,
Oncotarget
,PubMed ID 28977912
[PBRM1]
|
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223997L3 | Lenti-ORF clone of PBRM1 (Myc-DDK-tagged)-Human polybromo 1 (PBRM1), transcript variant 2 |
CNY 17,670.00 |
|
RC223997L4 | Lenti-ORF clone of PBRM1 (mGFP-tagged)-Human polybromo 1 (PBRM1), transcript variant 2 |
CNY 17,670.00 |
|
RG223997 | PBRM1 (tGFP-tagged) - Human polybromo 1 (PBRM1), transcript variant 2 |
CNY 17,390.00 |
|
SC308861 | PBRM1 (untagged)-Human polybromo 1 (PBRM1), transcript variant 2 |
CNY 30,500.00 |