Baf180 (PBRM1) (NM_018313) Human Untagged Clone
CAT#: SC308861
PBRM1 (untagged)-Human polybromo 1 (PBRM1), transcript variant 2
CNY 30,500.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | BAF180; PB1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC308861 representing NM_018313.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGTTCCAAGAGAAGAAGAGCTACCTCCCCTTCCAGCAGTGTCAGCGGGGACTTTGATGATGGGCAC CATTCTGTGTCAACACCAGGCCCAAGCAGGAAAAGGAGGAGACTTTCCAATCTTCCAACTGTAGATCCT ATTGCCGTGTGCCATGAACTCTATAATACCATCCGAGACTATAAGGATGAACAGGGCAGACTTCTCTGT GAGCTCTTCATTAGGGCACCAAAGCGAAGAAATCAACCAGACTATTATGAAGTGGTTTCTCAGCCCATT GACTTGATGAAAATCCAACAGAAACTAAAAATGGAAGAGTATGATGATGTTAATTTGCTGACTGCTGAC TTCCAGCTTCTTTTTAACAATGCAAAGTCCTATTATAAGCCAGATTCTCCTGAATATAAAGCCGCTTGC AAACTCTGGGATTTGTACCTTCGAACAAGAAATGAGTTTGTTCAGAAAGGAGAAGCAGATGACGAAGAT GATGATGAAGATGGGCAAGACAATCAGGGCACAGTGACTGAAGGATCTTCTCCAGCTTACTTGAAGGAG ATCCTGGAGCAGCTTCTTGAAGCCATAGTTGTAGCTACAAATCCATCAGGACGTCTCATTAGCGAACTT TTTCAGAAACTGCCTTCTAAAGTGCAATATCCAGATTATTATGCAATAATTAAGGAGCCTATAGATCTC AAGACCATTGCCCAGAGGATACAGAATGGAAGCTACAAAAGTATTCATGCAATGGCCAAAGATATAGAT CTCCTCGCAAAAAATGCCAAAACTTATAATGAGCCTGGCTCTCAAGTATTCAAGGATGCAAATTCAATT AAAAAAATATTTTATATGAAAAAGGCTGAAATTGAACATCATGAAATGGCTAAGTCAAGTCTTCGAATG AGGACTCCATCCAACTTGGCTGCAGCCAGACTGACAGGTCCTTCACACAGTAAAGGCAGCCTTGGTGAA GAGAGAAATCCCACTAGCAAGTATTACCGTAATAAAAGAGCAGTACAAGGAGGTCGTTTATCAGCAATT ACAATGGCACTTCAATATGGCTCAGAAAGTGAAGAAGATGCTGCTTTAGCTGCTGCACGCTATGAAGAG GGAGAGTCAGAAGCAGAAAGCATCACTTCCTTTATGGATGTTTCAAATCCTTTTTATCAGCTTTATGAC ACAGTTAGGAGTTGTCGGAATAACCAAGGGCAGCTAATAGCTGAACCTTTTTACCATTTGCCTTCAAAG AAAAAATACCCTGATTATTACCAGCAAATTAAAATGCCCATATCACTACAACAGATCCGAACAAAACTG AAGAATCAAGAATATGAAACTTTAGATCATTTGGAGTGTGATCTGAATTTAATGTTTGAAAATGCCAAA CGCTATAATGTGCCCAATTCAGCCATCTACAAGCGAGTTCTAAAATTGCAGCAAGTTATGCAGGCAAAG AAGAAAGAGCTTGCCAGGAGAGACGATATCGAGGACGGAGACAGCATGATCTCTTCAGCCACCTCTGAT ACTGGTAGTGCCAAAAGAAAAAGTAAAAAGAACATAAGAAAGCAGCGAATGAAAATCTTATTCAATGTT GTTCTTGAAGCTCGAGAGCCAGGTTCAGGCAGAAGACTTTGTGACCTATTTATGGTTAAACCATCCAAA AAGGACTATCCTGATTATTATAAAATCATCTTGGAGCCAATGGACTTGAAAATAATTGAGCATAACATC CGCAATGACAAATATGCTGGTGAAGAGGGAATGATAGAAGACATGAAGCTGATGTTCCGGAATGCCAGG CACTATAATGAGGAGGGCTCCCAGGTTTATAATGATGCACATATCCTGGAGAAGTTACTCAAGGAGAAA AGGAAAGAGCTGGGCCCACTGCCTGATGATGATGACATGGCTTCTCCCAAACTCAAGCTGAGTAGGAAG AGTGGCATTTCTCCTAAAAAATCAAAATACATGACTCCAATGCAGCAGAAACTAAATGAGGTCTATGAA GCTGTAAAGAACTATACTGATAAGAGGGGTCGCCGCCTCAGTGCCATATTTCTGAGGCTTCCCTCTAGA TCTGAGTTGCCTGACTACTATCTGACTATTAAAAAGCCCATGGACATGGAAAAAATTCGAAGTCACATG ATGGCCAACAAGTACCAAGATATTGACTCTATGGTTGAGGACTTTGTCATGATGTTTAATAATGCCTGT ACATACAATGAGCCGGAGTCTTTGATCTACAAAGATGCTCTTGTTCTACACAAAGTCCTGCTTGAAACA CGCAGAGACCTGGAGGGAGATGAGGACTCTCATGTCCCAAATGTGACTTTGCTGATTCAAGAGCTTATC CACAATCTTTTTGTGTCAGTCATGAGTCATCAGGATGATGAGGGAAGATGCTACAGCGATTCTTTAGCA GAAATTCCTGCTGTGGATCCCAACTTTCCTAACAAACCACCCCTTACATTTGACATAATTAGGAAGAAT GTTGAAAATAATCGCTACCGTCGGCTTGATTTATTTCAAGAGCATATGTTTGAAGTATTGGAACGAGCA AGAAGGATGAATCGGACAGATTCAGAAATATATGAAGATGCAGTAGAACTTCAGCAGTTTTTTATTAAA ATTCGTGATGAACTCTGCAAAAATGGAGAGATTCTTCTTTCACCGGCACTCAGCTATACCACAAAACAT TTGCATAATGATGTGGAGAAAGAGAGAAAGGAAAAATTGCCAAAAGAAATAGAGGAAGATAAACTAAAA CGAGAAGAAGAAAAAAGAGAAGCTGAAAAGAGTGAAGATTCCTCTGGTGCTGCAGGCCTCTCAGGCTTA CATCGCACATACAGCCAGGACTGTAGCTTTAAAAACAGCATGTACCATGTTGGAGATTACGTCTATGTG GAACCTGCAGAGGCCAACCTACAACCACATATCGTCTGTATTGAAAGACTGTGGGAGGATTCAGCTGAA AAAGAAGTTTTTAAGAGTGACTATTACAACAAAGTTCCAGTTAGTAAAATTCTAGGCAAGTGTGTGGTC ATGTTTGTCAAGGAATACTTTAAGTTATGCCCAGAAAACTTCCGAGATGAGGATGTTTTTGTCTGTGAA TCACGGTATTCTGCCAAAACCAAATCTTTTAAGAAAATTAAACTGTGGACCATGCCCATCAGCTCAGTC AGGTTTGTCCCTCGGGATGTGCCTCTGCCTGTGGTTCGCGTGGCCTCTGTATTTGCAAATGCAGATAAA GGTGATGATGAGAAGAATACAGACAACTCAGAGGACAGTCGAGCTGAAGACAATTTTAACTTGGAAAAG GAAAAAGAAGATGTCCCTGTGGAAATGTCCAATGGTGAACCAGGTTGCCACTACTTTGAGCAGCTCCAT TACAATGACATGTGGCTGAAGGTTGGCGACTGTGTCTTCATCAAGTCCCATGGCCTGGTGCGTCCTCGT GTGGGCAGAATTGAAAAAGTATGGGTTCGAGATGGAGCTGCATATTTTTATGGCCCCATCTTCATTCAC CCAGAAGAAACAGAGCATGAGCCCACAAAAATGTTCTACAAAAAAGAAGTATTTCTGAGTAATCTGGAA GAAACCTGCCCCATGACATGTATTCTCGGAAAGTGTGCTGTGTTGTCATTCAAGGACTTCCTCTCCTGC AGGCCAACTGAAATACCAGAAAATGACATTCTGCTTTGTGAGAGCCGCTACAATGAGAGCGACAAGCAG ATGAAGAAATTCAAAGGATTGAAGAGGTTTTCACTCTCTGCTAAAGTGGTAGATGATGAAATTTACTAC TTCAGAAAACCAATTGTTCCTCAGAAGGAGCCATCACCTTTGCTGGAAAAGAAGATCCAGTTGCTAGAA GCTAAATTTGCCGAGTTAGAAGGTGGAGATGATGATATTGAAGAGATGGGAGAAGAAGATAGTGAGTCT ACCCCAAAGTCTGCCAAAGGCAGTGCAAAGAAGGAAGGCTCCAAACGGAAAATCAACATGAGTGGCTAC ATCCTGTTCAGCAGTGAGATGAGGGCTGTGATTAAGGCCCAACACCCAGACTACTCTTTCGGGGAGCTC AGCCGCCTGGTGGGGACAGAATGGAGAAATCTTGAGACAGCCAAGAAAGCAGAATATGAAGGCATGATG GGTGGCTATCCGCCAGGCCTTCCACCTTTGCAGGGCCCAGTTGATGGCCTTGTTAGCATGGGCAGCATG CAGCCACTTCACCCTGGGGGGCCTCCACCCCACCATCTTCCGCCAGGTGTGCCTGGCCTCCCGGGCATC CCACCACCGGGTGTGATGAACCAAGGAGTGGCCCCTATGGTAGGGACTCCAGCACCAGGTGGAAGTCCA TATGGACAACAGGTGGGAGTTTTGGGGCCTCCAGGGCAGCAGGCACCACCTCCATATCCCGGCCCACAT CCAGCTGGACCCCCTGTCATACAGCAGCCAACAACACCCATGTTTGTAGCTCCCCCACCAAAGACCCAG CGGCTTCTTCACTCAGAGGCCTACCTGAAATACATTGAAGGACTCAGTGCGGAGTCCAACAGCATTAGC AAGTGGGATCAGACACTGGCAGCTCGAAGACGCGACGTCCATTTGTCGAAAGAACAGGAGAGCCGCCTA CCCTCTCACTGGCTGAAAAGCAAAGGGGCCCACACCACCATGGCAGATGCCCTCTGGCGCCTTCGAGAT TTGATGCTCCGGGACACCCTCAACATTCGCCAAGCATACAACCTAGAAAATGTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_018313 |
Insert Size | 4749 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_018313.4 |
RefSeq Size | 7592 bp |
RefSeq ORF | 4749 bp |
Locus ID | 55193 |
UniProt ID | Q86U86 |
Domains | BAH |
Protein Families | Druggable Genome |
MW | 181.1 kDa |
Gene Summary | This locus encodes a subunit of ATP-dependent chromatin-remodeling complexes. The encoded protein has been identified as in integral component of complexes necessary for ligand-dependent transcriptional activation by nuclear hormone receptors. Mutations at this locus have been associated with primary clear cell renal cell carcinoma. [provided by RefSeq, Feb 2012] Transcript Variant: This variant (2) contains multiple differences in the coding region, compared to variant 5. It encodes isoform 2, which is shorter than isoform 5. Variants 2 and 4 encode isoforms that are of the same length, but have distinct protein sequences. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223997 | PBRM1 (Myc-DDK-tagged)-Human polybromo 1 (PBRM1), transcript variant 2 |
CNY 10,824.00 |
|
RC223997L3 | Lenti-ORF clone of PBRM1 (Myc-DDK-tagged)-Human polybromo 1 (PBRM1), transcript variant 2 |
CNY 17,670.00 |
|
RC223997L4 | Lenti-ORF clone of PBRM1 (mGFP-tagged)-Human polybromo 1 (PBRM1), transcript variant 2 |
CNY 17,670.00 |
|
RG223997 | PBRM1 (tGFP-tagged) - Human polybromo 1 (PBRM1), transcript variant 2 |
CNY 17,390.00 |