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UACA (NM_001008224) Human Tagged ORF Clone
CAT＃: RC222247
- TrueORF®
UACA (Myc-DDK-tagged)-Human uveal autoantigen with coiled-coil domains and ankyrin repeats (UACA), transcript variant 2
ORF Plasmid: tGFP
"NM_001008224" in other vectors (4)
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CNY 9,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NUCLING |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222247 representing NM_001008224
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAACTGTTGGTTTTCTTGTACTCCTAAGAACAGACATGCAGCAGATTGGAATAAATATGATGACC GATTGATGAAAGCAGCAGAAAGGGGGGATGTAGAAAAAGTGACCTCAATCCTTGCTAAAAAGGGGGTCAA TCCAGGCAAACTAGATGTGGAAGGCAGATCTGTCTTCCATGTTGTGACCTCAAAGGGGAATCTTGAGTGT TTGAATGCCATCCTTATACATGGAGTTGATATTACAACCAGTGACACTGCAGGGAGAAATGCTCTTCACC TGGCTGCTAAGTATGGACATGCATTGTGCCTACAAAAACTTCTACAGTACAATTGTCCCACTGAGCATGC AGACCTGCAGGGAAGAACTGCACTTCACGATGCCGCAATGGCAGATTGTCCTTCTAGCATACAGCTGCTT TGTGACCATGGGGCCTCTGTGAATGCCAAAGATGTAGACGGGCGGACACCACTTGTTCTGGCTACTCAGA TGAGTAGGCCAACAATATGTCAACTGCTGATAGATAGAGGAGCGGATGTTAATTCCAGAGACAAACAAAA CAGAACTGCCCTCATGCTAGGTTGCGAATATGGTTGCAGAGATGCAGTAGAAGTCTTAATTAAAAATGGT GCTGATATAAGCTTGCTGGATGCGCTTGGCCATGATAGTTCTTACTATGCAAGAATTGGTGACAATCTGG ACATTCTAACCTTGTTGAAGACTGCATCGGAAAATACCAACAAAGGGAGAGAACTTTGGAAGAAAGGGCC ATCTTTGCAACAGCGAAATTTGACACACATGCAAGATGAAGTAAATGTGAAGTCACATCAGAGGGAGCAT CAAAATATTCAGGATTTGGAGATTGAAAATGAAGATTTGAAAGAGAGGTTGAGAAAAATTCAGCAAGAAC AAAGAATACTTTTGGATAAAGTCAATGGTTTACAGTTACAGCTGAATGAGGAAGTTATGGTTGCTGATGA TCTGGAAAGCGAGAGAGAAAAGCTGAAGTCCCTTTTGGCAGCTAAAGAAAAGCAACATGAAGAAAGCTTA AGGACTATTGAGGCTCTGAAAAATAGATTTAAATATTTTGAGAGTGATCATTTAGGATCAGGAAGTCATT TCAGTAACCGAAAAGAAGATATGCTTCTTAAACAAGGTCAGATGTATATGGCAGACTCACAGTGTACTTC CCCAGGTATACCAGCCCATATGCAAAGCAGATCTATGTTAAGACCTCTGGAACTATCTTTACCCAGTCAA ACGTCATACTCTGAAAATGAAATTTTAAAGAAAGAGTTAGAAGCAATGCGAACTTTCTGTGAGTCAGCAA AACAAGACCGACTGAAGCTCCAAAATGAACTGGCACACAAAGTGGCAGAATGCAAAGCTTTAGCATTAGA ATGTGAAAGGGTCAAGGAGGATTCAGATGAACAGATAAAGCAATTAGAAGATGCATTAAAAGATGTGCAG AAGAGGATGTATGAGTCAGAAGGTAAAGTTAAACAAATGCAGACCCATTTTCTTGCCCTTAAAGAACACT TAACAAGTGAAGCAGCCTCAGGGAATCACAGACTAACCGAGGAACTGAAGGATCAGTTGAAAGACTTGAA AGTAAAATATGAAGGTGCTTCAGCAGAAGTGGGGAAATTAAGAAACCAAATCAAACAAAATGAGATGATA GTAGAAGAGTTTAAGAGGGATGAAGGCAAGCTGATAGAAGAAAATAAGCGATTACAGAAGGAACTTAGTA TGTGTGAAATGGAGCGAGAGAAGAAAGGAAGAAAGGTCACAGAGATGGAAGGCCAGGCAAAAGAATTGTC AGCGAAGTTGGCCCTTTCCATTCCAGCTGAAAAATTTGAAAACATGAAGAGCTCATTATCAAATGAAGTG AATGAGAAAGCAAAAAAATTAGTAGAAATGGAAAGAGAACATGAAAAATCACTTAGTGAAATTAGACAGT TAAAGAGAGAACTTGAGAATGTTAAGGCCAAGCTTGCTCAGCACGTCAAACCAGAGGAACATGAACAGGT TAAGAGCAGATTAGAACAGAAATCAGGAGAACTTGGGAAGAAGATCACTGAGTTAACATTGAAAAATCAG ACACTACAAAAGGAAATTGAAAAAGTTTATTTGGATAATAAGCTCCTCAAGGAGCAAGCACATAACTTAA CAATTGAAATGAAAAATCATTATGTTCCTTTAAAAGTAAGTGAAGACATGAAAAAGTCACATGATGCAAT TATTGATGATCTTAATAGAAAGCTTTTAGATGTAACACAAAAATATACAGAAAAGAAGTTGGAAATGGAG AAATTGCTACTGGAAAATGACAGCTTAAGTAAGGATGTAAGCCGCCTAGAAACTGTGTTTGTACCTCCTG AGAAACATGAAAAAGAGATAATAGCTCTGAAATCCAATATTGTTGAACTTAAGAAACAGCTGTCTGAACT TAAGAAAAAATGTGGTGAAGACCAGGAGAAAATACACGCTCTCACATCTGAAAACACTAACTTGAAGAAG ATGATGAGTAATCAGTATGTGCCAGTTAAAACCCATGAAGAGGTTAAAATGACACTGAATGACACGTTAG CCAAAACTAACAGAGAATTATTAGATGTGAAGAAAAAATTTGAAGATATAAATCAGGAATTTGTAAAAAT AAAAGATAAGAATGAAATATTAAAAAGAAACCTGGAAAACACTCAGAACCAAATAAAAGCTGAGTACATC AGCCTGGCAGAGCACGAGGCAAAGATGAGCTCGCTAAGTCAGAGCATGAGAAAGGTGCAGGATAGTAATG CTGAAATCTTGGCCAACTACAGAAAAGGCCAAGAAGAGATTGTGACACTGCATGCCGAAATTAAAGCCCA GAAGAAGGAGCTCGACACAATACAAGAATGCATTAAGGTAAAATACGCCCCAATTGTCAGCTTTGAGGAG TGCGAGAGAAAATTTAAAGCAACAGAGAAAGAACTAAAAGACCAGTTATCAGAGCAGACACAAAAGTATA GTGTCAGTGAAGAAGAAGTCAAGAAAAACAAGCAAGAGAATGACAAGTTAAAGAAGGAGATTTTTACCCT TCAGAAAGATTTGAGAGATAAGACAGTTCTCATTGAGAAGTCTCATGAAATGGAAAGAGCATTAAGCAGA AAAACAGACGAGCTAAACAAACAGTTAAAAGACTTGTCACAGAAATACACGGAAGTAAAGAATGTGAAAG AGAAGCTAGTAGAAGAAAATGCCAAACAGACTTCTGAGATACTTGCAGTGCAAAATCTTTTGCAAAAACA ACATGTTCCATTGGAACAGGTTGAGGCTCTGAAAAAATCTCTTAATGGCACAATTGAAAATCTAAAGGAA GAACTGAAGAGTATGCAAAGGTGTTACGAGAAAGAGCAGCAGACAGTGACCAAACTGCATCAATTGTTGG AGAATCAAAAGAACTCTTCTGTACCCCTGGCAGAGCATTTGCAGATTAAAGAAGCATTTGAGAAAGAAGT TGGAATCATAAAAGCCAGCTTGAGAGAAAAGGAAGAAGAAAGCCAAAACAAAATGGAAGAAGTCTCCAAA CTTCAGTCGGAGGTTCAGAATACTAAACAAGCATTAAAAAAATTAGAGACTAGAGAGGTAGTTGACTTGT CTAAATATAAAGCAACAAAAAGTGATTTGGAGACACAGATTTCTAGCTTAAATGAAAAATTGGCCAATCT GAATAGAAAGTATGAGGAAGTATGTGAGGAAGTTTTGCATGCCAAAAAGAAGGAAATATCTGCAAAAGAT GAGAAGGAATTACTGCATTTCAGCATTGAGCAAGAAATTAAGGATCAGAAGGAACGATGTGATAAGTCCT TAACAACAATCACAGAGTTACAAAGAAGAATACAAGAATCTGCTAAACAAATAGAAGCAAAAGATAATAA GATAACTGAACTGCTTAATGATGTGGAAAGATTAAAACAGGCACTCAATGGCCTTTCCCAACTCACCTAC ACAAGTGGGAACCCCACCAAGAGGCAGAGCCAGCTGATTGACACTCTGCAGCACCAAGTGAAATCTCTGG AGCAACAGCTGGCCGATGCTGACAGACAGCACCAAGAAGTAATTGCAATTTATCGGACACACCTTCTTAG TGCTGCACAGGGTCACATGGATGAAGATGTTCAGGAGGCTCTGCTCCAGATCATACAAATGCGGCAGGGG CTTGTGTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222247 representing NM_001008224
Red=Cloning site Green=Tags(s) MMNCWFSCTPKNRHAADWNKYDDRLMKAAERGDVEKVTSILAKKGVNPGKLDVEGRSVFHVVTSKGNLEC LNAILIHGVDITTSDTAGRNALHLAAKYGHALCLQKLLQYNCPTEHADLQGRTALHDAAMADCPSSIQLL CDHGASVNAKDVDGRTPLVLATQMSRPTICQLLIDRGADVNSRDKQNRTALMLGCEYGCRDAVEVLIKNG ADISLLDALGHDSSYYARIGDNLDILTLLKTASENTNKGRELWKKGPSLQQRNLTHMQDEVNVKSHQREH QNIQDLEIENEDLKERLRKIQQEQRILLDKVNGLQLQLNEEVMVADDLESEREKLKSLLAAKEKQHEESL RTIEALKNRFKYFESDHLGSGSHFSNRKEDMLLKQGQMYMADSQCTSPGIPAHMQSRSMLRPLELSLPSQ TSYSENEILKKELEAMRTFCESAKQDRLKLQNELAHKVAECKALALECERVKEDSDEQIKQLEDALKDVQ KRMYESEGKVKQMQTHFLALKEHLTSEAASGNHRLTEELKDQLKDLKVKYEGASAEVGKLRNQIKQNEMI VEEFKRDEGKLIEENKRLQKELSMCEMEREKKGRKVTEMEGQAKELSAKLALSIPAEKFENMKSSLSNEV NEKAKKLVEMEREHEKSLSEIRQLKRELENVKAKLAQHVKPEEHEQVKSRLEQKSGELGKKITELTLKNQ TLQKEIEKVYLDNKLLKEQAHNLTIEMKNHYVPLKVSEDMKKSHDAIIDDLNRKLLDVTQKYTEKKLEME KLLLENDSLSKDVSRLETVFVPPEKHEKEIIALKSNIVELKKQLSELKKKCGEDQEKIHALTSENTNLKK MMSNQYVPVKTHEEVKMTLNDTLAKTNRELLDVKKKFEDINQEFVKIKDKNEILKRNLENTQNQIKAEYI SLAEHEAKMSSLSQSMRKVQDSNAEILANYRKGQEEIVTLHAEIKAQKKELDTIQECIKVKYAPIVSFEE CERKFKATEKELKDQLSEQTQKYSVSEEEVKKNKQENDKLKKEIFTLQKDLRDKTVLIEKSHEMERALSR KTDELNKQLKDLSQKYTEVKNVKEKLVEENAKQTSEILAVQNLLQKQHVPLEQVEALKKSLNGTIENLKE ELKSMQRCYEKEQQTVTKLHQLLENQKNSSVPLAEHLQIKEAFEKEVGIIKASLREKEEESQNKMEEVSK LQSEVQNTKQALKKLETREVVDLSKYKATKSDLETQISSLNEKLANLNRKYEEVCEEVLHAKKKEISAKD EKELLHFSIEQEIKDQKERCDKSLTTITELQRRIQESAKQIEAKDNKITELLNDVERLKQALNGLSQLTY TSGNPTKRQSQLIDTLQHQVKSLEQQLADADRQHQEVIAIYRTHLLSAAQGHMDEDVQEALLQIIQMRQG LVC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001008224 |
| ORF Size | 4209 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001008224.3 |
| RefSeq Size | 7096 bp |
| RefSeq ORF | 4212 bp |
| Locus ID | 55075 |
| UniProt ID | Q9BZF9 |
| MW | 161.3 kDa |
| Gene Summary | This gene encodes a protein that contains ankyrin repeats and coiled coil domains and likely plays a role in apoptosis. Studies in rodents have implicated the encoded protein in the stimulation of apoptosis and the regulation of mammary gland involution, in which the mammary gland returns to its pre-pregnant state. This protein has also been proposed to negatively regulate apoptosis based on experiments in human cell lines in which the protein was shown to interact with PRKC apoptosis WT1 regulator protein, also known as PAR-4, and inhibit translocation of the PAR-4 receptor. Autoantibodies to this protein have been identified in human patients with panuveitis and Graves' disease. Differential expression of this gene has been observed in various human cancers. [provided by RefSeq, May 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222247L3 | Lenti-ORF clone of UACA (Myc-DDK-tagged)-Human uveal autoantigen with coiled-coil domains and ankyrin repeats (UACA), transcript variant 2 |
CNY 15,870.00 |
|
| RC222247L4 | Lenti-ORF clone of UACA (mGFP-tagged)-Human uveal autoantigen with coiled-coil domains and ankyrin repeats (UACA), transcript variant 2 |
CNY 15,870.00 |
|
| RG222247 | UACA (tGFP-tagged) - Human uveal autoantigen with coiled-coil domains and ankyrin repeats (UACA), transcript variant 2 |
CNY 15,390.00 |
|
| SC301265 | UACA (untagged)-Human uveal autoantigen with coiled-coil domains and ankyrin repeats (UACA), transcript variant 2 |
CNY 27,080.00 |
