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TIGIT (NM_173799) Human Tagged ORF Clone
CAT＃: RC221447
TIGIT (Myc-DDK-tagged)-Human T cell immunoreceptor with Ig and ITIM domains (TIGIT)
ORF Plasmid: tGFP
"NM_173799" in other vectors (6)
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CNY 3,600.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | VSIG9; VSTM3; WUCAM |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221447 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCTGGTGTCTCCTCCTGATCTGGGCCCAGGGGCTGAGGCAGGCTCCCCTCGCCTCAGGAATGATGA CAGGCACAATAGAAACAACGGGGAACATTTCTGCAGAGAAAGGTGGCTCTATCATCTTACAATGTCACCT CTCCTCCACCACGGCACAAGTGACCCAGGTCAACTGGGAGCAGCAGGACCAGCTTCTGGCCATTTGTAAT GCTGACTTGGGGTGGCACATCTCCCCATCCTTCAAGGATCGAGTGGCCCCAGGTCCCGGCCTGGGCCTCA CCCTCCAGTCGCTGACCGTGAACGATACAGGGGAGTACTTCTGCATCTATCACACCTACCCTGATGGGAC GTACACTGGGAGAATCTTCCTGGAGGTCCTAGAAAGCTCAGTGGCTGAGCACGGTGCCAGGTTCCAGATT CCATTGCTTGGAGCCATGGCCGCGACGCTGGTGGTCATCTGCACAGCAGTCATCGTGGTGGTCGCGTTGA CTAGAAAGAAGAAAGCCCTCAGAATCCATTCTGTGGAAGGTGACCTCAGGAGAAAATCAGCTGGACAGGA GGAATGGAGCCCCAGTGCTCCCTCACCCCCAGGAAGCTGTGTCCAGGCAGAAGCTGCACCTGCTGGGCTC TGTGGAGAGCAGCGGGGAGAGGACTGTGCCGAGCTGCATGACTACTTCAATGTCCTGAGTTACAGAAGCC TGGGTAACTGCAGCTTCTTCACAGAGACTGGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221447 protein sequence
Red=Cloning site Green=Tags(s) MRWCLLLIWAQGLRQAPLASGMMTGTIETTGNISAEKGGSIILQCHLSSTTAQVTQVNWEQQDQLLAICN ADLGWHISPSFKDRVAPGPGLGLTLQSLTVNDTGEYFCIYHTYPDGTYTGRIFLEVLESSVAEHGARFQI PLLGAMAATLVVICTAVIVVVALTRKKKALRIHSVEGDLRRKSAGQEEWSPSAPSPPGSCVQAEAAPAGL CGEQRGEDCAELHDYFNVLSYRSLGNCSFFTETG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_173799 |
| ORF Size | 732 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_173799.4 |
| RefSeq Size | 2978 bp |
| RefSeq ORF | 735 bp |
| Locus ID | 201633 |
| UniProt ID | Q495A1 |
| Protein Families | Transmembrane |
| MW | 26.3 kDa |
| Gene Summary | This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.[provided by RefSeq, Sep 2009] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Development of Anti-human T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) Monoclonal Antibodies for Flow Cytometry
,Takei, J;Asano, T;Nanamiya, R;Nakamura, T;Yanaka, M;Hosono, H;Tanaka, T;Sano, M;Kaneko, MK;Harada, H;Kato, Y;,
Monoclonal antibodies in immunodiagnosis and immunotherapy
,PubMed ID 33900817
[TIGIT]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221447L1 | Lenti ORF clone of Human T cell immunoreceptor with Ig and ITIM domains (TIGIT), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC221447L2 | Lenti ORF clone of Human T cell immunoreceptor with Ig and ITIM domains (TIGIT), mGFP tagged |
CNY 5,890.00 |
|
| RC221447L3 | Lenti ORF clone of Human T cell immunoreceptor with Ig and ITIM domains (TIGIT), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC221447L4 | Lenti ORF clone of Human T cell immunoreceptor with Ig and ITIM domains (TIGIT), mGFP tagged |
CNY 6,000.00 |
|
| RG221447 | TIGIT (tGFP-tagged) - Human T cell immunoreceptor with Ig and ITIM domains (TIGIT) |
CNY 5,200.00 |
|
| SC124474 | TIGIT (untagged)-Human T cell immunoreceptor with Ig and ITIM domains (TIGIT) |
CNY 3,600.00 |
