SIPA1L3 (NM_015073) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CTRCT45; SPAL3; SPAR3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC220237 representing NM_015073 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCACCTATCGGGCCATCCCCAGCGATGGTGTGGACCTGGCAGCCAGCTGTGGCGCCAGGGTGGGCG ATGTCCTCCCTGGGCCACACACAGGGGACTACGCTCCCTTGGGATTCTGGGCCCAGAATGGCAGCATGTC CCAGCCTCTTGGCGAGAGCCCGGCCACCGCCACCGCCACCGCCACCGCCACCACCCGCCCCAGCCCCACC ACTCCCGCAATGCCCAAGATGGGCGTGCGCGCAAGGGTGGCCGACTGGCCGCCCAAGCGGGAGGCCCTGA GAGAGCACAGCAACCCAAGCCCCTCCCAGGACACAGATGGCACAAAGGCCACCAAGATGGCCCATTCCAT GAGGAGCATACAGAACGGACAGCCCCCCACCAGCACCCCGGCTTCCTCAGGGTCCAAAGCCTTCCACCGA CTCTCCAGGAGAAGGTCCAAAGACGTGGAGTTCCAGGACGGGTGGCCCCGGTCCCCCGGCAGGGCCTTCC TCCCCCTTCGGCACCGCAGCAGCAGCGAGATCACCCTCAGCGAGTGTGACGCGGAGGACGCGGGGGAGCC GCGGGGGGCCCGGCACACGGGGGCGCTGCCCCTCTTCCGCGAGTACGGGAGCACCTCGTCCATCGACGTG CAGGGCATGCCCGAGCAGAGCTTCTTCGACATCCTGAACGAGTTCCGCAGCGAGCAGCCCGACGCCCGAG GGTGCCAGGCCCTCACCGAGCTCCTCCGGGCAGATCCTGGCCCACACCTCATGGGGGGCGGCGGCGGAGC CAAGGGGGACTCCCACAACGGGCAGCCCGCCAAGGACAGCCTCCTGCCACTGCAGCCCACGAAGGAGAAG GAGAAGGCCCGGAAGAAACCTGCGCGGGGCCTCGGCGGCGGGGACACGGTGGACTCGTCCATCTTTCGGA AGCTAAGGAGCAGCAAACCCGAGGGGGAGGCTGGGCGTTCCCCGGGGGAGGCCGACGAGGGCCGGAGCCC CCCGGAAGCCAGCAGGCCGTGGGTGTGTCAGAAGAGCTTCGCCCACTTCGACGTGCAGAGCATGCTGTTC GACCTCAACGAGGCGGCCGCCAACAGGGTGTCGGTGTCGCAGCGGCGGAACACCACCACGGGTGCTTCGG CCGCTTCCGCCGCCTCGGCCATGGCCTCCCTCACGGCCTCGCGGGCCCACAGCCTCGGAGGCCTGGACCC GGCCTTCACCAGCACAGAGGACCTAAACTGCAAGGAGAACTTGGAGCAGGACCTCGGCGATGACAACAGC AACGACCTGCTGCTCAGCTGCCCGCACTTCCGCAATGAGATCGGGGGCGAGTGTGAGCGCAACGTGAGCT TCTCCCGGGCTTCCGTGGGCTCCCCGAGCAGCGGCGAGGGCCACCTGGCAGAGCCCGCCCTGAGCGCCTA CCGCACCAACGCCAGCATCTCGGTGTTGGAAGTTCCCAAGGAGCAGCAGCGGACGCAGAGTCGGCCCCGG CAGTACAGCATCGAGCATGTGGACCTGGGCGCCCGCTACTACCAGGATTACTTCGTGGGCAAAGAACATG CCAATTACTTCGGCGTGGATGAGAAGCTGGGGCCAGTGGCTGTGAGCATTAAGCGGGAGAAGCTGGAAGA CCACAAGGAGCACGGACCTCAGTACCAGTACAGGATCATCTTCCGGACCCGCGAGCTCATCACCCTGCGG GGCTCCATCCTGGAAGATGCTACGCCCACAGCCACCAAGCATGGGACCGGGCGGGGCCTGCCCTTGAAGG ATGCCCTGGAGTATGTCATCCCCGAGCTCAACATCCACTGCCTGCGGCTGGCCCTCAACACCCCCAAGGT GACGGAGCAACTGCTGAAGCTCGATGAGCAAGGGCTCTGCCGGAAGCACAAGGTGGGCATCCTCTATTGC AAGGCCGGCCAGAGCTCCGAGGAGGAGATGTACAACAATGAGGAGGCCGGCCCCGCCTTTGAGGAGTTCC TCTCCCTCATCGGCGAGAAGGTCTGCCTGAAGGGCTTCACCAAGTACGCTGCCCAGCTGGACGTCAAGAC CGACTCCACGGGAACCCACTCCCTCTACACGATGTACCAGGACTACGAGATCATGTTCCATGTCTCCACC CTGCTCCCTTACACCCCCAACAACAGGCAGCAGCTGCTACGGAAGAGGCACATAGGAAATGACATCGTGA CGATCATCTTCCAGGAGCCTGGCGCGCTACCGTTCACCCCCAAGAACATCCGCTCCCACTTCCAGCACGT CTTCATCATTGTCCGAGTCCACAACCCCTGCACTGATAACGTCTGTTACAGTATGGCTGTGACCCGATCC AAAGACGCTCCTCCTTTCGGCCCCCCCATCCCCAGTGGAACCACATTCCGCAAATCCGACGTCTTCAGAG ACTTCTTGCTGGCCAAGGTGATTAACGCTGAGAACGCCGCGCACAAGTCCGACAAGTTCCACACCATGGC CACCAGGACCCGCCAGGAGTATCTCAAGGACCTGGCCGAAAACTGTGTCTCCAACACCCCCATCGACTCC ACCGGCAAATTCAACCTCATCTCCCTGACCTCCAAGAAGAAGGAAAAGACAAAAGCACGGGCTGGCGCTG AGCAGCACAGTGCAGGGGCCATCGCCTGGAGGGTGGTGGCCCAGGACTACGCCCAGGGGGTGGAAATCGA CTGCATTTTGGGAATTTCCAATGAGTTTGTGGTGCTCCTGGACTTACGCACCAAGGAGGTGGTGTTCAAC TGCTACTGCGGGGATGTCATTGGCTGGACTCCAGACTCCTCCACACTCAAAATCTTCTATGGACGAGGAG ACCACATCTTCCTACAGGCGACAGAGGGTTCTGTGGAGGACATAAGGGAGATAGTGCAGAGACTGAAGGT GATGACCAGTGGCTGGGAGACGGTGGACATGACGCTTCGGCGGAACGGGCTCGGGCAGCTGGGCTTCCAC GTGAAGTACGACGGCACGGTGGCCGAGGTTGAGGACTATGGGTTCGCCTGGCAGGCCGGCCTCCGGCAGG GCAGCCGACTAGTGGAGATCTGCAAGGTGGCCGTGGTCACACTGACCCACGACCAGATGATCGACCTGCT GCGCACCTCTGTCACTGTGAAGGTGGTCATCATCCCGCCTTTTGAGGACGGCACTCCCCGGAGGGGTTGG CCGGAGACCTACGACATGAATACCTCGGAGCCCAAGACGGAGCAGGAAAGCATCACTCCTGGGGGCCGGC CCCCCTACCGCAGCAATGCTCCCTGGCAGTGGAGCGGGCCCGCATCCCATAACTCTCTACCAGCCTCCAA GTGGGCCACTCCAACCACTCCCGGCCATGCCCAGTCCCTGAGCCGGCCCCTGAAGCAGACCCCCATAGTC CCCTTCCGGGAGTCCCAGCCACTGCACAGCAAGAGGCCTGTCAGCTTCCCAGAAACCCCTTACACAGTAT CACCAGCAGGGGCCGACAGAGTCCCTCCCTACCGACAGCCTTCTGGGAGCTTCTCCACCCCCGGTTCGGC CACCTACGTGAGATACAAGCCATCCCCAGAAAGGTACACGGCTGCCCCACACCCCCTGCTATCTCTTGAT CCCCACTTCAGCCACGATGGGACGTCCAGCGGCGACTCCTCTTCCGGCGGCCTGACCAGCCAGGAGAGCA CCATGGAACGCCAGAAGCCAGAGCCTTTGTGGCATGTGCCTGCCCAGGCCAGGCTCTCAGCCATAGCCGG AAGCAGCGGGAACAAGCACCCGTCCAGGCAGGATGCAGCAGGCAAAGATTCTCCCAACAGGCATTCCAAA GGAGAACCTCAATACTCAAGTCATTCCAGCAGCAACACCCTCTCCAGCAACGCATCCAGCAGCCACAGCG ACGACCGCTGGTTCGACCCCCTGGACCCCCTGGAGCCAGAGCAAGACCCCCTCTCCAAGGGTGGCTCTAG TGACAGCGGCATCGACACCACCCTCTACACCTCCAGCCCTAGCTGCATGTCCCTGGCCAAGGCTCCACGG CCCGCCAAGCCACACAAGCCCCCTGGAAGTATGGGCCTTTGTGGCGGGGGTCGCGAGGCCGCTGGGAGGT CCCACCACGCAGACAGGCGGCGGGAGGTCTCCCCTGCCCCCGCAGTTGCCGGCCAAAGCAAGGGCTACCG ACCGAAGCTGTACTCCTCCGGCTCCAGCACCCCCACGGGACTGGCGGGGGGCAGCCGAGACCCACCGAGG CAGCCCAGTGACATGGGCTCGAGGGTTGGCTACCCCGCTCAGGTTTACAAAACTGCCAGTGCAGAGACTC CTCGGCCCTCCCAGCTGGCCCAGCCCAGCCCCTTTCAGCTCTCCGCCTCCGTCCCCAAGTCCTTCTTCTC CAAGCAGCCTGTACGCAATAAGCACCCAACAGGGTGGAAGAGAACGGAGGAGCCCCCACCACGGCCACTC CCCTTCAGTGACCCAAAGAAGCAGGTGGACACGAACACCAAAAATGTCTTTGGGCAACCGAGGTTGAGGG CATCCCTCCGAGACCTCCGGTCACCACGGAAGAACTACAAATCCACCATCGAGGATGACCTGAAGAAACT CATCATCATGGACAACCTGGGGCCAGAGCAGGAGAGAGACACGGGACAGTCACCGCAGAAGGGCCTGCAG CGGACGCTGTCGGACGAGAGCCTGTGCAGCGGGCGCCGGGAGCCCAGCTTCGCCAGCCCCGCTGGCCTAG AGCCAGGGCTGCCCAGCGACGTGCTCTTCACCAGCACCTGCGCCTTCCCGTCCAGCACGCTGCCTGCACG CCGCCAGCACCAGCACCCCCACCCGCCCGTCGGCCCCGGTGCCACCCCTGCCGCCGGCAGCGGCTTTCCC GAGAAGAAATCCACCATCTCAGCCTCGGAGCTCTCGCTGGCTGATGGGCGGGACCGCCCCCTGCGGCGCC TGGACCCTGGGCTGATGCCCCTGCCTGACACAGCTGCTGGCCTCGAGTGGTCCAGCCTGGTGAACGCAGC CAAGGCATACGAAGTGCAAAGAGCCGTCTCACTCTTCTCTCTGAACGACCCGGCCCTGAGCCCGGACATC CCGCCTGCACACAGTCCTGTCCACAGCCACCTGAGCCTGGAGAGGGGACCCCCGACCCCCAGGACCACCC CTACCATGAGCGAGGAGCCACCCCTGGATCTGACAGGCAAGGTGTACCAGCTGGAGGTGATGCTGAAACA GCTGCACACTGACCTGCAGAAGGAGAAGCAGGACAAGGTGGTGCTCCAGTCAGAGGTGGCCAGCCTGCGG CAGAACAACCAGCGGCTGCAGGAGGAGTCGCAGGCCGCCAGCGAGCAGCTGCGCAAGTTTGCGGAGATCT TCTGCAGGGAGAAGAAGGAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC220237 representing NM_015073 Red=Cloning site Green=Tags(s) MTTYRAIPSDGVDLAASCGARVGDVLPGPHTGDYAPLGFWAQNGSMSQPLGESPATATATATATTRPSPT TPAMPKMGVRARVADWPPKREALREHSNPSPSQDTDGTKATKMAHSMRSIQNGQPPTSTPASSGSKAFHR LSRRRSKDVEFQDGWPRSPGRAFLPLRHRSSSEITLSECDAEDAGEPRGARHTGALPLFREYGSTSSIDV QGMPEQSFFDILNEFRSEQPDARGCQALTELLRADPGPHLMGGGGGAKGDSHNGQPAKDSLLPLQPTKEK EKARKKPARGLGGGDTVDSSIFRKLRSSKPEGEAGRSPGEADEGRSPPEASRPWVCQKSFAHFDVQSMLF DLNEAAANRVSVSQRRNTTTGASAASAASAMASLTASRAHSLGGLDPAFTSTEDLNCKENLEQDLGDDNS NDLLLSCPHFRNEIGGECERNVSFSRASVGSPSSGEGHLAEPALSAYRTNASISVLEVPKEQQRTQSRPR QYSIEHVDLGARYYQDYFVGKEHANYFGVDEKLGPVAVSIKREKLEDHKEHGPQYQYRIIFRTRELITLR GSILEDATPTATKHGTGRGLPLKDALEYVIPELNIHCLRLALNTPKVTEQLLKLDEQGLCRKHKVGILYC KAGQSSEEEMYNNEEAGPAFEEFLSLIGEKVCLKGFTKYAAQLDVKTDSTGTHSLYTMYQDYEIMFHVST LLPYTPNNRQQLLRKRHIGNDIVTIIFQEPGALPFTPKNIRSHFQHVFIIVRVHNPCTDNVCYSMAVTRS KDAPPFGPPIPSGTTFRKSDVFRDFLLAKVINAENAAHKSDKFHTMATRTRQEYLKDLAENCVSNTPIDS TGKFNLISLTSKKKEKTKARAGAEQHSAGAIAWRVVAQDYAQGVEIDCILGISNEFVVLLDLRTKEVVFN CYCGDVIGWTPDSSTLKIFYGRGDHIFLQATEGSVEDIREIVQRLKVMTSGWETVDMTLRRNGLGQLGFH VKYDGTVAEVEDYGFAWQAGLRQGSRLVEICKVAVVTLTHDQMIDLLRTSVTVKVVIIPPFEDGTPRRGW PETYDMNTSEPKTEQESITPGGRPPYRSNAPWQWSGPASHNSLPASKWATPTTPGHAQSLSRPLKQTPIV PFRESQPLHSKRPVSFPETPYTVSPAGADRVPPYRQPSGSFSTPGSATYVRYKPSPERYTAAPHPLLSLD PHFSHDGTSSGDSSSGGLTSQESTMERQKPEPLWHVPAQARLSAIAGSSGNKHPSRQDAAGKDSPNRHSK GEPQYSSHSSSNTLSSNASSSHSDDRWFDPLDPLEPEQDPLSKGGSSDSGIDTTLYTSSPSCMSLAKAPR PAKPHKPPGSMGLCGGGREAAGRSHHADRRREVSPAPAVAGQSKGYRPKLYSSGSSTPTGLAGGSRDPPR QPSDMGSRVGYPAQVYKTASAETPRPSQLAQPSPFQLSASVPKSFFSKQPVRNKHPTGWKRTEEPPPRPL PFSDPKKQVDTNTKNVFGQPRLRASLRDLRSPRKNYKSTIEDDLKKLIIMDNLGPEQERDTGQSPQKGLQ RTLSDESLCSGRREPSFASPAGLEPGLPSDVLFTSTCAFPSSTLPARRQHQHPHPPVGPGATPAAGSGFP EKKSTISASELSLADGRDRPLRRLDPGLMPLPDTAAGLEWSSLVNAAKAYEVQRAVSLFSLNDPALSPDI PPAHSPVHSHLSLERGPPTPRTTPTMSEEPPLDLTGKVYQLEVMLKQLHTDLQKEKQDKVVLQSEVASLR QNNQRLQEESQAASEQLRKFAEIFCREKKEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_015073
ORF Size	5343 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_015073.1, NP_055888.1
RefSeq Size	7984 bp
RefSeq ORF	5346 bp
Locus ID	23094
UniProt ID	O60292
MW	194.6 kDa
Gene Summary	This gene belongs to the signal induced proliferation associated 1 family of genes, which encode GTPase-activating proteins specific for the GTP-binding protein Rap1. Rap1 has been implicated in regulation of cell adhesion, cell polarity, and organization of the cytoskeleton. Like other members of the family, the protein encoded by this gene contains RapGAP and PDZ domains. In addition, this protein contains a C-terminal leucine zipper domain. This gene is proposed to function in epithelial cell morphogenesis and establishment or maintenance of polarity. Consistently, expression of the protein in cell culture showed localization to cell-cell borders in apical regions, and downregulation of the gene in 3D Caco2 cell culture resulted in abnormal cell polarity and morphogenesis. Allelic variants of this gene have been associated with congenital cataracts in humans. [provided by RefSeq, Feb 2016]

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