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ULK3 (NM_001099436) Human Tagged ORF Clone
CAT＃: RC220209
ULK3 (Myc-DDK-tagged)-Human unc-51-like kinase 3 (C. elegans) (ULK3)
ORF Plasmid: tGFP
"NM_001099436" in other vectors (7)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220209 representing NM_001099436
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGGCCCGGCTGGGGTCCCCCGCGCCTGGACGGCTTCATCCTCACCGAGCGCCTGGGCAGCGGCA CGTACGCCACGGTGTACAAGGCCTACGCCAAGAAGGACACTCGTGAAGTGGTAGCCATAAAGTGTGTAGC CAAGAAAAGTCTGAACAAGGCATCGGTGGAGAACCTCCTCACGGAGATTGAGATCCTCAAGGGCATTCGA CATCCCCACATTGTGCAGCTGAAAGACTTTCAGTGGGACAGTGACAATATCTACCTCATCATGGAGTTTT GCGCAGGGGGCGACCTGTCTCGCTTCATCCATACCCGCAGGATTCTGCCTGAGAAGGTGGCGCGTGTCTT CATGCAGCAATTAGCTAGCGCCCTGCAATTCCTGCATGAACGGAATATCTCTCACCTGGATCTGAAGCCA CAGAACATTCTACTGAGCTCCTTGGAGAAGCCCCACCTAAAACTGGCAGACTTTGGTTTCGCACAACACA TGTCCCCGTGGGATGAGAAGCACGTGCTCCGTGGCTCCCCCCTCTACATGGCCCCCGAGATGGTGTGCCA GCGGCAGTATGACGCCCGCGTGGACCTCTGGTCCATGGGGGTCATCCTGTATGAAGCCCTCTTCGGGCAG CCCCCCTTTGCCTCCAGGTCGTTCTCGGAGCTGGAAGAGAAGATCCGTAGCAACCGGGTCATCGAGCTCC CCTTGCGGCCCCTGCTCTCCCGAGACTGCCGGGACCTACTGCAGCGGCTCCTGGAGCGGGACCCCAGCCG TCGCATCTCCTTCCAGGACTTTTTTGCGCACCCCTGGGTGGACCTGGAGCACATGCCCAGTGGGGAGAGT CTGGGGCGAGCAACCGCCCTGGTGGTGCAGGCTGTGAAGAAAGACCAGGAGGGGGATTCAGCAGCTGCCT TATCACTCTACTGCAAGGCTCTGGACTTCTTTGTACCTGCCCTGCACTATGAAGTGGATGCCCAGCGGAA GGAGGCAATTAAGGCAAAGGTGGGGCAGTACGTGTCCCGGGCTGAGGAGCTCAAGGCCATCGTCTCCTCT TCCAATCAGGCCCTGCTGAGGCAGGGGACCTCTGCCCGAGACCTGCTCAGAGAGATGGCCCGGGACAAGC CACGCCTCCTAGCTGCCCTGGAAGTGGCTTCAGCTGCCATGGCCAAGGAGGAGGCCGCCGGCGGGGAGCA GGATGCCCTGGACCTGTACCAGCACAGCCTGGGGGAGCTACTGCTGTTGCTGGCAGCGGAGCCCCCGGGC CGGAGGCGGGAGCTGCTTCACACTGAGGTTCAGAACCTCATGGCCCGAGCTGAATACTTGAAGGAGCAGG TCAAGATGAGGGAATCTCGCTGGGAAGCTGACACCCTGGACAAAGAGGGACTGTCGGAATCTGTTCGTAG CTCTTGCACCCTTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220209 representing NM_001099436
Red=Cloning site Green=Tags(s) MAGPGWGPPRLDGFILTERLGSGTYATVYKAYAKKDTREVVAIKCVAKKSLNKASVENLLTEIEILKGIR HPHIVQLKDFQWDSDNIYLIMEFCAGGDLSRFIHTRRILPEKVARVFMQQLASALQFLHERNISHLDLKP QNILLSSLEKPHLKLADFGFAQHMSPWDEKHVLRGSPLYMAPEMVCQRQYDARVDLWSMGVILYEALFGQ PPFASRSFSELEEKIRSNRVIELPLRPLLSRDCRDLLQRLLERDPSRRISFQDFFAHPWVDLEHMPSGES LGRATALVVQAVKKDQEGDSAAALSLYCKALDFFVPALHYEVDAQRKEAIKAKVGQYVSRAEELKAIVSS SNQALLRQGTSARDLLREMARDKPRLLAALEVASAAMAKEEAAGGEQDALDLYQHSLGELLLLLAAEPPG RRRELLHTEVQNLMARAEYLKEQVKMRESRWEADTLDKEGLSESVRSSCTLQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001099436 |
| ORF Size | 1416 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001099436.4 |
| RefSeq Size | 2635 bp |
| RefSeq ORF | 1419 bp |
| Locus ID | 25989 |
| UniProt ID | Q6PHR2 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | mTOR signaling pathway, Regulation of autophagy |
| MW | 53.3 kDa |
| Gene Summary | Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220209L1 | Lenti ORF clone of Human unc-51-like kinase 3 (C. elegans) (ULK3), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC220209L2 | Lenti ORF clone of Human unc-51-like kinase 3 (C. elegans) (ULK3), mGFP tagged |
CNY 5,890.00 |
|
| RC220209L3 | Lenti ORF clone of Human unc-51-like kinase 3 (C. elegans) (ULK3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220209L4 | Lenti ORF clone of Human unc-51-like kinase 3 (C. elegans) (ULK3), mGFP tagged |
CNY 5,890.00 |
|
| RG220209 | ULK3 (tGFP-tagged) - Human unc-51-like kinase 3 (C. elegans) (ULK3) |
CNY 4,370.00 |
|
| SC316533 | ULK3 (untagged)-Human unc-51-like kinase 3 (C. elegans) (ULK3) |
CNY 7,220.00 |
|
| SC323667 | ULK3 (untagged)-Kinase deficient mutant (K87M) of Human unc-51-like kinase 3 (C. elegans) (ULK3) |
CNY 3,656.00 |
