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RWDD3 (NM_015485) Human Tagged Lenti ORF Clone
CAT＃: RC217602L4
- LentiORF®
Lenti ORF clone of Human RWD domain containing 3 (RWDD3), transcript variant 1, mGFP tagged
"NM_015485" in other vectors (7)
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CNY 5,890.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | RSUME |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC217602).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015485 |
| ORF Size | 801 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015485.4, NP_056300.2 |
| RefSeq Size | 1250 bp |
| RefSeq ORF | 804 bp |
| Locus ID | 25950 |
| Domains | RWD |
| MW | 30.3 kDa |
| Gene Summary | Enhancer of SUMO conjugation. Via its interaction with UBE2I/UBC9, increases SUMO conjugation to proteins by promoting the binding of E1 and E2 enzymes, thioester linkage between SUMO and UBE2I/UBC9 and transfer of SUMO to specific target proteins which include HIF1A, PIAS, NFKBIA, NR3C1 and TOP1. Isoform 1 and isoform 2 positively regulate the NF-kappa-B signaling pathway by enhancing the sumoylation of NF-kappa-B inhibitor alpha (NFKBIA), promoting its stabilization which consequently leads to an increased inhibition of NF-kappa-B transcriptional activity. Isoform 1 and isoform 2 negatively regulate the hypoxia-inducible factor-1 alpha (HIF1A) signaling pathway by increasing the sumoylation of HIF1A, promoting its stabilization, transcriptional activity and the expression of its target gene VEGFA during hypoxia. Isoform 2 promotes the sumoylation and transcriptional activity of the glucocorticoid receptor NR3C1 and enhances the interaction of SUMO1 and NR3C1 with UBE2I/UBC9. Has no effect on ubiquitination.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217602 | RWDD3 (Myc-DDK-tagged)-Human RWD domain containing 3 (RWDD3), transcript variant 1 |
CNY 2,400.00 |
|
| RC217602L1 | Lenti ORF clone of Human RWD domain containing 3 (RWDD3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217602L2 | Lenti ORF clone of Human RWD domain containing 3 (RWDD3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC217602L3 | Lenti ORF clone of Human RWD domain containing 3 (RWDD3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RG217602 | RWDD3 (tGFP-tagged) - Human RWD domain containing 3 (RWDD3), transcript variant 1 |
CNY 4,000.00 |
|
| SC114654 | RWDD3 (untagged)-Human RWD domain containing 3 (RWDD3), transcript variant 1 |
CNY 2,400.00 |
|
| SC317385 | RWDD3 (untagged)-Human RWD domain containing 3 (RWDD3), transcript variant 1 |
CNY 6,270.00 |
