United States
Germany
Japan
United Kingdom
China
RWDD3 (NM_015485) Human Untagged Clone
CAT＃: SC114654
RWDD3 (untagged)-Human RWD domain containing 3 (RWDD3), transcript variant 1
CNY 2,400.00
CNY 6,270.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | RSUME |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114654 sequence for NM_015485 edited (data generated by NextGen Sequencing)
ATGGCGGAGCCTGTGCAGGAGGAGCTCTCGGTCCTGGCCGCGATTTTCTGCAGGCCCCAC GAGTGGGAGGTGCTGAGCCGCTCAGAGACAGATGGGACCGTGTTCAGAATTCACACAAAA GCTGAAGGATTTATGGATGCGGATATACCTCTGGAATTGGTGTTCCATTTGCCAGTCAAT TATCCTTCATGTCTACCTGGTATCTCGATTAACTCTGAACAGTTGACCAGGGCCCAGTGT GTGACTGTGAAAGAGAAGTTACTTGAGCAAGCAGAGAGCCTTTTGTCGGAGCCTATGGTT CATGAGCTGGTTCTCTGGATTCAGCAGAATCTCAGGCATATCCTCAGCCAACCAGAAACT GGCAGTGGCAGTGAAAAGTGTACTTTTTCAACAAGCACGACCATGGATGATGGATTGTGG ATAACTCTTTTGCATTTAGATCACATGAGAGCAAAGACTAAATATGTCAAAATTGTGGAG AAGTGGGCTTCAGATTTAAGGCTGACAGGAAGACTGATGTTCATGGGTAAAATAATACTG ATTTTACTACAGGGAGACAGAAACAACCTCAAGGNNNNNNNNNTTCTTCAGAAAACCTCC AAAGTAGATGTGGACTCAAGTGGAAAGAAATGCAAAGAGAAAATGATTAGTGTACTGTTT GAAACAAAAGTACAGACAGAACACAAAAGGTTTCTGGCATTTGAAGTCAAAGAGTATTCA GCGTTGGATGAATTACAAAAGGAATTTGAAACTGCAGGACTTAAGAAGCTTTTCTCCGAA TTTGTACTTGCTCTGGTAAAATGA Clone variation with respect to NM_015485.4 258 t=>g;575 a=>n;576 g=>n;577 t=>n;578 a=>n;579 c=>n;580 t=>n;581 t=>n;582 g=>n;583 a=>n >OriGene 5' read for NM_015485 unedited
NNNGGGTTCAAAATTTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGGGA AGCGCTGAGGCGGTGGGGCCCACAGCCATGGCGGAGCCTGTGCAGGAGGAGCTCTCGGTC CTGGCCGCGATTTTCTGCAGGCCCCACGAGTGGGAGGTGCTGAGCCGCTCAGAGACAGAT GGGACCGTGTTCAGAATTCACACAAAAGCTGAAGGATTTATGGATGCGGATATACCTCTG GAATTGGTGTTCCATTTGCCAGTCAATTATCCTTCATGTCTACCTGGTATCTCGATTAAC TCTGAACAGTTGACCAGGGCCCAGTGTGTGACTGTGAAAGAGAAGTTACTTGAGCAAGCA GAGAGCCTTTTGTCGGAGCCTATGGTTCATGAGCTGGTTCTCTGGATTCAGCAGAATCTC AGGCATATCCTCAGCCAACCAGAAACTGGCAGTGGCAGTGAAAAGTGTACTTTTTCAACA AGCACGACCATGGATGATGGATTGTGGATAACTCTTTTGCATTTAGATCACATGAGAGCA AAGACTAAATATGTCAAAATTGTGGAGAAGTGGGCTTCAGATTTAAGGCTGACAGGAAGA CTGATGTTCATGGGTAAAATAATACTGATTTTACTACAGGGAGACAGAAACAACCTCAAG GTGCCAAAAAGTTAAATGTTGAGTATGAATCTGGCTATTTTCTGCTTTAAATGGTGTGTC TTTAAGTGTGTTTTATAACAATGGGATAGATTAATTATTAAGATGTTTCTGCTTTCATTA TTACAATCTTAATGGATCTTCCTTTTCTTTTTAAAGAATGTCTGACTGCNTATTACAAGT ACAAACTTGCCAAGCTTGAAGATAAGATTGCATTTTAAAATCTC >OriGene 3' read for NM_015485 unedited
ACCGCGGGCCGCAATCTAGAGTCGAGGNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTAAAAAAATCTTTTATTTCCAAAAAAATTCCCCAAAAAATTCCTTTT TTTCAAACAAATTTTGTTTTTAAAAACAGGGGGCCTTTCCCCATTTTTTAAAATTTATTT TTAAAAAAAGGGCTAATTTACGGAACCTACCCTTTCCGGGAAAACATTAAAAAATACAAG TTTTTTTCAGGTTAAAAATTCCATAAAACAAAACGGGTTTTTGTCCCTGAAGTAAAAAGG AGCCCTATCTTTTGGCTTCAAAAAAACTTTAAAATGGCCTTTTTAAATAACCCCCCCCCC AAACCCAGGGCAAAACCAACAAAAAACCCGGTTTTTTTACAAAAAAATCCCGGGCTTCCA TTTCATTTACCCAAAGCAGGTCCAAATTCGGAAAAAAGCTTTTTAAGCCCTGCAGTTCCA AATCCCTTTGGAAATCCACCCAACGCTGAAAACTTTTTGACTTCAAAGGCCAAAACCCTG CTTTATACAAAAAAACCTCAGGAATATCCCTAGATTTGCCAAAGTATAATTATGGATTCA GTGGCTTTTCCATCGGCAAAAGAACTAAATTATCCCTTTGGGGTTCGGCCTGACCTTTGG TTACAAACAGAACACTAATCATTTTCTTTTTGCATTTCTTTCCCCTGGAGCCCACATTTA CTTGTGGAGGTTTTCCTGAAGAATCAAGGCCCTCCTAAATAAATAACCCGGCACCTTTAA AATCCTTTCTTTACTTTGAGCCCCAAAGGCCCCAGTTTAAAAGAAGCTTTAACACGTGGA GCGTTATTTTCATGCTTAAAACCCCCCTTTTTAAACCAAGCAGGAAGAATTTTATATCCT TGTTTACAATCCTGGGCAGAAAAGGGTAGGGGCGGTCCCGGAATAAAGACAATATACCCA CCTCCCAGGNTTAAACGGGATGACCGCTACAAGTTCTACCTGGGCGGGCACTAAAACTGT GGGACAGCGCCTTCCAN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_015485 |
| Insert Size | 3270 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015485.3, NP_056300.1 |
| RefSeq Size | 1093 bp |
| RefSeq ORF | 588 bp |
| Locus ID | 25950 |
| Domains | RWD |
| Gene Summary | Enhancer of SUMO conjugation. Via its interaction with UBE2I/UBC9, increases SUMO conjugation to proteins by promoting the binding of E1 and E2 enzymes, thioester linkage between SUMO and UBE2I/UBC9 and transfer of SUMO to specific target proteins which include HIF1A, PIAS, NFKBIA, NR3C1 and TOP1. Isoform 1 and isoform 2 positively regulate the NF-kappa-B signaling pathway by enhancing the sumoylation of NF-kappa-B inhibitor alpha (NFKBIA), promoting its stabilization which consequently leads to an increased inhibition of NF-kappa-B transcriptional activity. Isoform 1 and isoform 2 negatively regulate the hypoxia-inducible factor-1 alpha (HIF1A) signaling pathway by increasing the sumoylation of HIF1A, promoting its stabilization, transcriptional activity and the expression of its target gene VEGFA during hypoxia. Isoform 2 promotes the sumoylation and transcriptional activity of the glucocorticoid receptor NR3C1 and enhances the interaction of SUMO1 and NR3C1 with UBE2I/UBC9. Has no effect on ubiquitination.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (a). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217602 | RWDD3 (Myc-DDK-tagged)-Human RWD domain containing 3 (RWDD3), transcript variant 1 |
CNY 2,400.00 |
|
| RC217602L1 | Lenti ORF clone of Human RWD domain containing 3 (RWDD3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217602L2 | Lenti ORF clone of Human RWD domain containing 3 (RWDD3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC217602L3 | Lenti ORF clone of Human RWD domain containing 3 (RWDD3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217602L4 | Lenti ORF clone of Human RWD domain containing 3 (RWDD3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG217602 | RWDD3 (tGFP-tagged) - Human RWD domain containing 3 (RWDD3), transcript variant 1 |
CNY 4,000.00 |
|
| SC317385 | RWDD3 (untagged)-Human RWD domain containing 3 (RWDD3), transcript variant 1 |
CNY 6,270.00 |
