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PAPP A2 (PAPPA2) (NM_020318) Human Tagged ORF Clone
CAT＃: RC215960
- TrueORF®
PAPPA2 (Myc-DDK-tagged)-Human pappalysin 2 (PAPPA2), transcript variant 1
ORF Plasmid: tGFP
"NM_020318" in other vectors (5)
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CNY 18,056.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PAPP-A2; PAPP-E; PAPPE; PLAC3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215960 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTGCTTAAAGATCCTAAGAATAAGCCTGGCGATTTTGGCTGGGTGGGCACTCTGTTCTGCCAACT CTGAGCTGGGCTGGACACGCAAGAAATCCTTGGTTGAGAGGGAACACCTGAATCAGGTGCTGTTGGAAGG AGAACGTTGTTGGCTGGGGGCCAAGGTTCGAAGACCCAGAGCTTCTCCACAGCATCACCTCTTTGGAGTC TACCCCAGCAGGGCTGGGAACTACCTAAGGCCCTACCCCGTGGGGGAGCAAGAAATCCATCATACAGGAC GCAGCAAACCAGACACTGAAGGAAATGCTGTGAGCCTTGTTCCCCCAGACCTGACTGAAAATCCAGCAGG ACTGAGGGGTGCAGTTGAAGAGCCGGCTGCCCCATGGGTAGGGGATAGTCCTATTGGGCAATCTGAGCTG CTGGGAGATGATGACGCTTATCTCGGCAATCAAAGATCCAAGGAGTCTCTAGGTGAGGCCGGGATTCAGA AAGGCTCAGCCATGGCTGCCACTACTACCACCGCCATTTTCACAACCCTGAACGAACCCAAACCAGAGAC CCAAAGGAGGGGCTGGGCCAAGTCCAGGCAGCGTCGCCAAGTGTGGAAGAGGCGGGCGGAAGATGGGCAG GGAGACTCCGGTATCTCTTCACATTTCCAACCTTGGCCCAAGCATTCCCTTAAACACAGGGTCAAAAAGA GTCCACCGGAGGAAAGCAACCAAAATGGTGGAGAGGGCTCCTACCGAGAAGCAGAGACCTTTAACTCCCA AGTAGGACTGCCCATCTTATACTTCTCTGGGAGGCGGGAGCGGCTGCTGCTGCGTCCAGAAGTGCTGGCT GAGATTCCCCGGGAGGCGTTCACAGTGGAAGCCTGGGTTAAACCGGAGGGAGGACAGAACAACCCAGCCA TCATCGCAGGTGTGTTTGATAACTGCTCCCACACTGTCAGTGACAAAGGCTGGGCCCTGGGGATCCGCTC AGGGAAGGACAAGGGAAAGCGGGATGCTCGCTTCTTCTTCTCCCTCTGCACCGACCGCGTGAAGAAAGCC ACCATCTTGATTAGCCACAGTCGCTACCAACCAGGCACATGGACCCATGTGGCAGCCACTTACGATGGAC GGCACATGGCCCTGTATGTGGATGGCACTCAGGTGGCTAGCAGTCTAGACCAGTCTGGTCCCCTGAACAG CCCCTTCATGGCATCTTGCCGCTCTTTGCTCCTGGGGGGAGACAGCTCTGAGGATGGGCACTATTTCCGT GGACACCTGGGCACACTGGTTTTCTGGTCGACCGCCCTGCCACAAAGCCATTTTCAGCACAGTTCTCAGC ATTCAAGTGGGGAGGAGGAAGCGACTGACTTGGTCCTGACAGCGAGCTTTGAGCCTGTGAACACAGAGTG GGTTCCCTTTAGAGATGAGAAGTACCCACGACTTGAGGTTCTCCAGGGCTTTGAGCCAGAGCCTGAGATT CTGTCGCCTTTGCAGCCCCCACTCTGTGGGCAAACAGTCTGTGACAATGTGGAATTGATCTCCCAGTACA ATGGATACTGGCCCCTTCGGGGAGAGAAGGTGATACGCTACCAGGTGGTGAACATCTGTGATGATGAGGG CCTAAACCCCATTGTGAGTGAGGAGCAGATTCGTCTGCAGCACGAGGCACTGAATGAGGCCTTCAGCCGC TACAACATCAGCTGGCAGCTGAGCGTCCACCAGGTCCACAATTCCACCCTGCGACACCGGGTTGTGCTTG TGAACTGTGAGCCCAGCAAGATTGGCAATGACCATTGTGACCCCGAGTGTGAGCACCCACTCACAGGCTA TGATGGGGGTGACTGCCGCCTGCAGGGCCGCTGCTACTCCTGGAACCGCAGGGATGGGCTCTGTCACGTG GAGTGTAACAACATGCTGAACGACTTTGACGACGGAGACTGCTGCGACCCCCAGGTGGCTGATGTGCGCA AGACCTGCTTTGACCCTGACTCACCCAAGAGGGCATACATGAGTGTGAAGGAGCTGAAGGAGGCCCTGCA GCTGAACAGTACTCACTTCCTCAACATCTACTTTGCCAGCTCAGTGCGGGAAGACCTTGCAGGTGCTGCC ACCTGGCCTTGGGACAAGGACGCTGTCACTCACCTGGGTGGCATTGTCCTCAGCCCAGCATATTATGGGA TGCCTGGCCACACCGACACCATGATCCATGAAGTGGGACATGTTCTGGGACTCTACCATGTCTTTAAAGG AGTCAGTGAAAGAGAATCCTGCAATGACCCCTGCAAGGAGACAGTGCCATCCATGGAAACGGGAGACCTC TGTGCCGACACCGCCCCCACTCCCAAGAGTGAGCTGTGCCGGGAACCAGAGCCCACTAGTGACACCTGTG GCTTCACTCGCTTCCCAGGGGCTCCGTTCACCAACTACATGAGCTACACGGATGATAACTGCACTGACAA CTTCACTCCTAACCAAGTGGCCCGAATGCATTGCTATTTGGACCTAGTCTATCAGCAGTGGACTGAAAGC AGAAAGCCCACCCCCATCCCCATTCCACCTATGGTCATCGGACAGACCAACAAGTCCCTCACTATCCACT GGCTGCCTCCTATTAGTGGAGTTGTATATGACAGGGCCTCAGGCAGCTTGTGTGGCGCTTGCACTGAAGA TGGGACCTTTCGTCAGTATGTGCACACAGCTTCCTCCCGGCGGGTGTGTGACTCCTCAGGTTATTGGACC CCAGAGGAGGCTGTGGGGCCTCCTGATGTGGATCAGCCCTGCGAGCCAAGCTTACAGGCCTGGAGCCCTG AGGTCCACCTGTACCACATGAACATGACGGTCCCCTGCCCCACAGAAGGCTGTAGCTTGGAGCTGCTCTT CCAACACCCGGTCCAAGCCGACACCCTCACCCTGTGGGTCACTTCCTTCTTCATGGAGTCCTCGCAGGTC CTCTTTGACACAGAGATCTTGCTGGAAAACAAGGAGTCAGTGCACCTGGGCCCCTTAGACACTTTCTGTG ACATCCCACTCACCATCAAACTGCACGTGGATGGGAAGGTGTCGGGGGTGAAAGTCTACACCTTTGATGA GAGGATAGAGATTGATGCAGCACTCCTGACTTCTCAGCCCCACAGTCCCTTGTGCTCTGGCTGCAGGCCT GTGAGGTACCAGGTTCTCCGCGATCCCCCATTTGCCAGTGGTTTGCCCGTGGTGGTGACACATTCTCACA GGAAGTTCACGGACGTGGAGGTCACACCTGGACAGATGTATCAGTACCAAGTTCTAGCTGAAGCTGGAGG AGAACTGGGAGAAGCTTCGCCTCCTCTGAACCACATTCATGGAGCTCCTTATTGTGGAGATGGGAAGGTG TCAGAGAGACTGGGAGAAGAGTGTGATGATGGAGACCTTGTGAGCGGAGATGGCTGCTCCAAGGTGTGTG AGCTGGAGGAAGGTTTCAACTGTGTAGGAGAGCCAAGCCTTTGCTACATGTATGAGGGAGATGGCATATG TGAACCTTTTGAGAGAAAAACCAGCATTGTAGACTGTGGCATCTACACTCCCAAAGGATACTTGGATCAA TGGGCTACCCGGGCTTACTCCTCTCATGAAGACAAGAAGAAGTGTCCTGTTTCCTTGGTAACTGGAGAAC CTCATTCCCTAATTTGCACATCATACCATCCAGATTTACCCAACCACCGTCCCCTAACTGGCTGGTTTCC CTGTGTTGCCAGTGAAAATGAAACTCAGGATGACAGGAGTGAACAGCCAGAAGGTAGCCTGAAGAAAGAG GATGAGGTTTGGCTCAAAGTGTGTTTCAATAGACCAGGAGAGGCCAGAGCAATTTTTATTTTTTTGACAA CTGATGGCCTAGTTCCCGGAGAGCATCAGCAGCCGACAGTGACTCTCTACCTGACCGATGTCCGTGGAAG CAACCACTCTCTTGGAACCTATGGACTGTCATGCCAGCATAATCCACTGATTATCAATGTGACCCATCAC CAGAATGTCCTTTTCCACCATACCACCTCAGTGCTGCTGAATTTCTCATCCCCACGGGTCGGCATCTCAG CTGTGGCTCTAAGGACATCCTCCCGCATTGGTCTTTCGGCTCCCAGTAACTGCATCTCAGAGGACGAGGG GCAGAATCATCAGGGACAGAGCTGTATCCATCGGCCCTGTGGGAAGCAGGACAGCTGTCCGTCATTGCTG CTTGATCATGCTGATGTGGTGAACTGTACCTCTATAGGCCCAGGTCTCATGAAGTGTGCTATCACTTGTC AAAGGGGATTTGCCCTTCAGGCCAGCAGTGGGCAGTACATCAGGCCCATGCAGAAGGAAATTCTGCTCAC ATGTTCTTCTGGGCACTGGGACCAGAATGTGAGCTGCCTTCCCGTGGACTGCGGTGTTCCCGACCCGTCT TTGGTGAACTATGCAAACTTCTCCTGCTCAGAGGGAACCAAATTTCTGAAACGCTGCTCAATCTCTTGTG TCCCACCAGCCAAGCTGCAAGGACTGAGCCCATGGCTGACATGTCTTGAAGATGGTCTCTGGTCTCTCCC TGAAGTCTACTGCAAGTTGGAGTGTGATGCTCCCCCTATTATTCTGAATGCCAACTTGCTCCTGCCTCAC TGCCTCCAGGACAACCACGACGTGGGCACCATCTGCAAATATGAATGCAAACCAGGGTACTATGTGGCAG AAAGTGCAGAGGGTAAAGTCAGGAACAAGCTCCTGAAGATACAATGCCTGGAAGGTGGAATCTGGGAGCA AGGCAGCTGCATTCCTGTGGTGTGTGAGCCACCCCCTCCTGTGTTTGAAGGCATGTATGAATGTACCAAT GGCTTCAGCCTGGACAGCCAGTGTGTGCTCAACTGTAACCAGGAACGTGAAAAGCTTCCCATCCTCTGCA CTAAAGAGGGCCTGTGGACCCAGGAGTTTAAGTTGTGTGAGAATCTGCAAGGAGAATGCCCACCACCCCC CTCAGAGCTGAATTCTGTGGAGTACAAATGTGAACAAGGATATGGGATTGGTGCAGTGTGTTCCCCATTG TGTGTAATCCCCCCCAGTGACCCCGTGATGCTACCTGAGAATATCACTGCTGACACTCTGGAGCACTGGA TGGAACCTGTCAAAGTCCAGAGCATTGTGTGCACTGGCCGGCGTCAATGGCACCCAGACCCCGTCTTAGT CCACTGCATCCAGTCATGTGAGCCCTTCCAAGCAGATGGTTGGTGTGACACTATCAACAACCGAGCCTAC TGCCACTATGACGGGGGAGACTGCTGCTCTTCCACACTCTCCTCCAAGAAGGTCATTCCATTTGCTGCTG ACTGTGACCTGGATGAGTGCACCTGCCGGGACCCCAAGGCAGAAGAAAATCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215960 protein sequence
Red=Cloning site Green=Tags(s) MMCLKILRISLAILAGWALCSANSELGWTRKKSLVEREHLNQVLLEGERCWLGAKVRRPRASPQHHLFGV YPSRAGNYLRPYPVGEQEIHHTGRSKPDTEGNAVSLVPPDLTENPAGLRGAVEEPAAPWVGDSPIGQSEL LGDDDAYLGNQRSKESLGEAGIQKGSAMAATTTTAIFTTLNEPKPETQRRGWAKSRQRRQVWKRRAEDGQ GDSGISSHFQPWPKHSLKHRVKKSPPEESNQNGGEGSYREAETFNSQVGLPILYFSGRRERLLLRPEVLA EIPREAFTVEAWVKPEGGQNNPAIIAGVFDNCSHTVSDKGWALGIRSGKDKGKRDARFFFSLCTDRVKKA TILISHSRYQPGTWTHVAATYDGRHMALYVDGTQVASSLDQSGPLNSPFMASCRSLLLGGDSSEDGHYFR GHLGTLVFWSTALPQSHFQHSSQHSSGEEEATDLVLTASFEPVNTEWVPFRDEKYPRLEVLQGFEPEPEI LSPLQPPLCGQTVCDNVELISQYNGYWPLRGEKVIRYQVVNICDDEGLNPIVSEEQIRLQHEALNEAFSR YNISWQLSVHQVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLQGRCYSWNRRDGLCHV ECNNMLNDFDDGDCCDPQVADVRKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNIYFASSVREDLAGAA TWPWDKDAVTHLGGIVLSPAYYGMPGHTDTMIHEVGHVLGLYHVFKGVSERESCNDPCKETVPSMETGDL CADTAPTPKSELCREPEPTSDTCGFTRFPGAPFTNYMSYTDDNCTDNFTPNQVARMHCYLDLVYQQWTES RKPTPIPIPPMVIGQTNKSLTIHWLPPISGVVYDRASGSLCGACTEDGTFRQYVHTASSRRVCDSSGYWT PEEAVGPPDVDQPCEPSLQAWSPEVHLYHMNMTVPCPTEGCSLELLFQHPVQADTLTLWVTSFFMESSQV LFDTEILLENKESVHLGPLDTFCDIPLTIKLHVDGKVSGVKVYTFDERIEIDAALLTSQPHSPLCSGCRP VRYQVLRDPPFASGLPVVVTHSHRKFTDVEVTPGQMYQYQVLAEAGGELGEASPPLNHIHGAPYCGDGKV SERLGEECDDGDLVSGDGCSKVCELEEGFNCVGEPSLCYMYEGDGICEPFERKTSIVDCGIYTPKGYLDQ WATRAYSSHEDKKKCPVSLVTGEPHSLICTSYHPDLPNHRPLTGWFPCVASENETQDDRSEQPEGSLKKE DEVWLKVCFNRPGEARAIFIFLTTDGLVPGEHQQPTVTLYLTDVRGSNHSLGTYGLSCQHNPLIINVTHH QNVLFHHTTSVLLNFSSPRVGISAVALRTSSRIGLSAPSNCISEDEGQNHQGQSCIHRPCGKQDSCPSLL LDHADVVNCTSIGPGLMKCAITCQRGFALQASSGQYIRPMQKEILLTCSSGHWDQNVSCLPVDCGVPDPS LVNYANFSCSEGTKFLKRCSISCVPPAKLQGLSPWLTCLEDGLWSLPEVYCKLECDAPPIILNANLLLPH CLQDNHDVGTICKYECKPGYYVAESAEGKVRNKLLKIQCLEGGIWEQGSCIPVVCEPPPPVFEGMYECTN GFSLDSQCVLNCNQEREKLPILCTKEGLWTQEFKLCENLQGECPPPPSELNSVEYKCEQGYGIGAVCSPL CVIPPSDPVMLPENITADTLEHWMEPVKVQSIVCTGRRQWHPDPVLVHCIQSCEPFQADGWCDTINNRAY CHYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020318 |
| ORF Size | 5373 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020318.3 |
| RefSeq Size | 6940 bp |
| RefSeq ORF | 5376 bp |
| Locus ID | 60676 |
| UniProt ID | Q9BXP8 |
| Protein Families | Protease, Secreted Protein |
| MW | 198.5 kDa |
| Gene Summary | This gene encodes a member of the pappalysin family of metzincin metalloproteinases. The encoded protein cleaves insulin-like growth factor-binding protein 5 and is thought to be a local regulator of insulin-like growth factor (IGF) bioavailability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
PAPPA2 as a Therapeutic Modulator of IGF-I Bioavailability: in Vivo and in Vitro Evidence
,Andrew, M;Liao, L;Fujimoto, M;Khoury, J;Hwa, V;Dauber, A;,
J Endocr Soc
,PubMed ID 29942928
[PAPPA2]
|
|
PAPPA2 as a Therapeutic Modulator of IGF-I Bioavailability: In-vivo and In-vitro Evidence
,Andrew, M;Liao, L;Fujimoto, M;Khoury, J;Hwa, V;Dauber, A;,
Journal of the Endocrine Society
[PAPPA2]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215960L1 | Lenti ORF clone of Human pappalysin 2 (PAPPA2), transcript variant 1, Myc-DDK-tagged |
CNY 20,456.00 |
|
| RC215960L3 | Lenti ORF clone of Human pappalysin 2 (PAPPA2), transcript variant 1, Myc-DDK-tagged |
CNY 16,060.00 |
|
| RG215960 | PAPPA2 (tGFP-tagged) - Human pappalysin 2 (PAPPA2), transcript variant 1 |
CNY 15,580.00 |
|
| SC125584 | PAPPA2 (untagged)-Human pappalysin 2 (PAPPA2), transcript variant 1 |
CNY 18,056.00 |
|
| SC318671 | PAPPA2 (untagged)-Human pappalysin 2 (PAPPA2), transcript variant 1 |
CNY 18,064.00 |
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