United States
Germany
Japan
United Kingdom
China
KCNH1 (NM_002238) Human Tagged ORF Clone
CAT＃: RC215104
KCNH1 (Myc-DDK-tagged)-Human potassium voltage-gated channel, subfamily H (eag-related), member 1 (KCNH1), transcript variant 2
ORF Plasmid: tGFP
"NM_002238" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 10,568.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EAG; EAG1; h-eag; hEAG; hEAG1; Kv10.1; TMBTS; ZLS1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215104 representing NM_002238
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCATGGCTGGGGGCAGGAGGGGACTAGTGGCCCCTCAAAACACGTTTCTGGAGAATATTGTTCGGC GGTCCAATGATACTAATTTTGTGTTGGGGAATGCTCAGATAGTGGACTGGCCTATTGTGTACAGCAATGA TGGATTTTGCAAGCTGTCTGGCTATCACAGGGCAGAAGTGATGCAAAAAAGCAGCACCTGCAGTTTTATG TATGGGGAGCTGACTGATAAAGACACGATTGAAAAAGTGCGGCAAACATTTGAGAACTATGAGATGAATT CCTTTGAAATTCTGATGTACAAGAAGAACAGGACACCTGTGTGGTTCTTTGTGAAAATTGCTCCAATTCG AAACGAACAGGATAAAGTGGTTTTATTTCTTTGCACTTTCAGTGACATAACAGCTTTCAAACAGCCAATT GAGGATGATTCATGTAAAGGCTGGGGGAAGTTTGCTCGGCTGACAAGAGCACTGACAAGCAGCAGGGGTG TCCTGCAGCAGCTGGCTCCAAGCGTGCAAAAAGGCGAGAATGTCCACAAGCACTCCCGCCTGGCAGAGGT CCTACAGCTGGGCTCAGACATCCTTCCCCAGTACAAGCAAGAGGCACCAAAGACTCCCCCTCACATCATC TTACATTATTGTGTTTTTAAGACCACGTGGGATTGGATCATCTTGATCTTGACCTTCTATACAGCCATCT TGGTCCCTTATAATGTCTCCTTCAAAACCAGGCAGAATAATGTGGCCTGGCTGGTTGTTGATAGCATCGT GGATGTTATCTTTTTGGTGGACATTGTGCTCAATTTTCATACCACCTTTGTTGGACCAGCAGGGGAGGTG ATTTCTGACCCCAAACTTATCCGCATGAACTACCTGAAGACGTGGTTTGTGATTGACCTTCTGTCCTGTT TGCCATATGATGTCATCAACGCTTTTGAGAACGTGGATGAGGGCATCAGCAGCCTGTTCAGCTCTCTAAA AGTTGTCCGGCTGCTCCGTCTTGGGCGAGTGGCCCGTAAGCTGGACCACTACATTGAATATGGAGCTGCT GTGCTGGTCCTGCTGGTGTGTGTGTTTGGGCTGGCTGCACACTGGATGGCCTGCATCTGGTACAGCATTG GGGACTATGAGATCTTTGACGAGGACACCAAGACAATCCGCAACAACAGCTGGCTGTACCAACTAGCGAT GGACATTGGCACCCCTTACCAGTTTAATGGGTCTGGCTCAGGGAAGTGGGAAGGTGGTCCCAGCAAGAAT TCTGTCTACATCTCCTCGTTGTATTTCACAATGACCAGCCTCACCAGTGTGGGCTTTGGGAACATCGCCC CATCCACAGACATTGAGAAGATCTTTGCAGTGGCCATCATGATGATTGGCTCACTTCTCTATGCCACCAT CTTCGGGAATGTGACGACTATTTTCCAACAGATGTATGCCAACACCAACAGATACCATGAGATGCTCAAC AGTGTTCGGGACTTCCTGAAGCTCTACCAGGTGCCAAAAGGATTGAGTGAGCGAGTAATGGATTATATTG TGTCCACTTGGTCCATGTCCAGAGGCATTGACACAGAGAAGGTCCTGCAGATCTGCCCCAAGGACATGAG AGCCGACATCTGCGTGCACCTGAACCGCAAGGTGTTCAAGGAGCACCCGGCCTTCCGGCTGGCCAGTGAT GGCTGCCTCCGGGCACTGGCCATGGAGTTCCAGACGGTGCACTGTGCCCCAGGGGACCTCATCTACCATG CAGGAGAGAGCGTTGACAGCCTCTGCTTTGTGGTTTCTGGCTCCCTGGAGGTGATCCAAGATGATGAGGT GGTGGCCATTCTAGGAAAAGGAGACGTGTTTGGAGATGTGTTCTGGAAGGAAGCCACCCTTGCCCAGTCC TGTGCCAATGTTAGGGCCTTGACCTACTGTGATCTGCATGTGATCAAGCGGGATGCCCTGCAGAAAGTGC TGGAATTCTACACGGCCTTCTCCCATTCCTTCTCCCGGAACCTGATTCTGACGTACAACTTGAGGAAGAG GATTGTGTTCCGGAAGATCAGCGACGTGAAACGTGAAGAGGAAGAACGCATGAAACGAAAGAATGAGGCC CCCCTGATCTTGCCCCCGGACCACCCTGTCCGGCGCCTCTTCCAGAGATTCCGACAGCAGAAAGAGGCCA GGCTGGCAGCTGAGAGAGGGGGCCGGGACCTGGATGACCTAGATGTGGAGAAGGGCAATGTCCTTACAGA GCATGCCTCCGCCAACCACAGCCTCGTGAAGGCCAGCGTGGTCACCGTGCGTGAGAGTCCTGCCACGCCC GTATCCTTCCAGGCAGCCTCCACCTCCGGGGTGCCAGACCACGCAAAGCTACAGGCGCCAGGGTCCGAGT GCCTGGGCCCCAAGGGGGGCGGGGGCGATTGTGCCAAGCGCAAAAGCTGGGCCCGCTTCAAAGATGCTTG CGGGAAGAGTGAGGACTGGAACAAGGTGTCCAAGGCTGAGTCGATGGAGACACTTCCCGAGAGGACAAAA GCGTCAGGCGAGGCCACACTGAAGAAGACAGACTCGTGTGACAGTGGCATCACCAAGAGCGACTTGCGCC TGGACAACGTGGGTGAGGCCAGGAGTCCCCAGGATCGGAGTCCCATCCTGGCAGAGGTCAAGCATTCGTT CTACCCCATCCCTGAGCAGACGCTGCAGGCCACAGTCCTGGAGGTGAGGCACGAGCTGAAGGAGGACATC AAGGCCTTAAACGCCAAAATGACCAATATTGAGAAACAGCTCTCTGAGATACTCAGGATATTAACTTCCA GAAGATCCTCTCAGTCTCCTCAGGAGTTGTTTGAAATATCGAGGCCACAGTCCCCAGAATCAGAGAGAGA CATTTTTGGAGCCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215104 representing NM_002238
Red=Cloning site Green=Tags(s) MTMAGGRRGLVAPQNTFLENIVRRSNDTNFVLGNAQIVDWPIVYSNDGFCKLSGYHRAEVMQKSSTCSFM YGELTDKDTIEKVRQTFENYEMNSFEILMYKKNRTPVWFFVKIAPIRNEQDKVVLFLCTFSDITAFKQPI EDDSCKGWGKFARLTRALTSSRGVLQQLAPSVQKGENVHKHSRLAEVLQLGSDILPQYKQEAPKTPPHII LHYCVFKTTWDWIILILTFYTAILVPYNVSFKTRQNNVAWLVVDSIVDVIFLVDIVLNFHTTFVGPAGEV ISDPKLIRMNYLKTWFVIDLLSCLPYDVINAFENVDEGISSLFSSLKVVRLLRLGRVARKLDHYIEYGAA VLVLLVCVFGLAAHWMACIWYSIGDYEIFDEDTKTIRNNSWLYQLAMDIGTPYQFNGSGSGKWEGGPSKN SVYISSLYFTMTSLTSVGFGNIAPSTDIEKIFAVAIMMIGSLLYATIFGNVTTIFQQMYANTNRYHEMLN SVRDFLKLYQVPKGLSERVMDYIVSTWSMSRGIDTEKVLQICPKDMRADICVHLNRKVFKEHPAFRLASD GCLRALAMEFQTVHCAPGDLIYHAGESVDSLCFVVSGSLEVIQDDEVVAILGKGDVFGDVFWKEATLAQS CANVRALTYCDLHVIKRDALQKVLEFYTAFSHSFSRNLILTYNLRKRIVFRKISDVKREEEERMKRKNEA PLILPPDHPVRRLFQRFRQQKEARLAAERGGRDLDDLDVEKGNVLTEHASANHSLVKASVVTVRESPATP VSFQAASTSGVPDHAKLQAPGSECLGPKGGGGDCAKRKSWARFKDACGKSEDWNKVSKAESMETLPERTK ASGEATLKKTDSCDSGITKSDLRLDNVGEARSPQDRSPILAEVKHSFYPIPEQTLQATVLEVRHELKEDI KALNAKMTNIEKQLSEILRILTSRRSSQSPQELFEISRPQSPESERDIFGAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002238 |
| ORF Size | 2886 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002238.4 |
| RefSeq Size | 3127 bp |
| RefSeq ORF | 2889 bp |
| Locus ID | 3756 |
| UniProt ID | O95259 |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| MW | 108.4 kDa |
| Gene Summary | Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming (alpha) subunit of a voltage-gated non-inactivating delayed rectifier potassium channel. It is activated at the onset of myoblast differentiation. The gene is highly expressed in brain and in myoblasts. Overexpression of the gene may confer a growth advantage to cancer cells and favor tumor cell proliferation. Alternative splicing of this gene results in two transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Determining the target of membrane sterols on voltage-gated potassium channels
,Zakany, F;Pap, P;Papp, F;Kovacs, T;Nagy, P;Peter, M;Szente, L;Panyi, G;Varga, Z;,
Biochim Biophys Acta Mol Cell Biol Lipids
,PubMed ID 30553843
[KCNH1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215104L1 | Lenti ORF clone of Human potassium voltage-gated channel, subfamily H (eag-related), member 1 (KCNH1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC215104L2 | Lenti ORF clone of Human potassium voltage-gated channel, subfamily H (eag-related), member 1 (KCNH1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC215104L3 | Lenti ORF clone of Human potassium voltage-gated channel, subfamily H (eag-related), member 1 (KCNH1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC215104L4 | Lenti ORF clone of Human potassium voltage-gated channel, subfamily H (eag-related), member 1 (KCNH1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG215104 | KCNH1 (tGFP-tagged) - Human potassium voltage-gated channel, subfamily H (eag-related), member 1 (KCNH1), transcript variant 2 |
CNY 4,370.00 |
|
| SC303154 | KCNH1 (untagged)-Human potassium voltage-gated channel, subfamily H (eag-related), member 1 (KCNH1), transcript variant 2 |
CNY 10,576.00 |
