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IFIT3 (NM_001031683) Human Tagged ORF Clone
CAT＃: RC214701
IFIT3 (Myc-DDK-tagged)-Human interferon-induced protein with tetratricopeptide repeats 3 (IFIT3), transcript variant 2
ORF Plasmid: tGFP
"NM_001031683" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
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CNY 300.00
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CIG-49; cig41; GARG-49; IFI60; IFIT4; IRG2; ISG60; P60; RIG-G |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214701 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGAGGTCACCAAGAATTCCCTGGAGAAAATCCTTCCACAGCTGAAATGCCATTTCACCTGGAACT TATTCAAGGAAGACAGTGTCTCAAGGGATCTAGAAGATAGAGTGTGTAACCAGATTGAATTTTTAAACAC TGAGTTCAAAGCTACAATGTACAACTTGTTGGCCTACATAAAACACCTAGATGGTAACAACGAGGCAGCC CTGGAATGCTTACGGCAAGCTGAAGAGTTAATCCAGCAAGAACATGCTGACCAAGCAGAAATCAGAAGTC TAGTCACTTGGGGAAACTACGCCTGGGTCTACTATCACTTGGGCAGACTCTCAGATGCTCAGATTTATGT AGATAAGGTGAAACAAACCTGCAAGAAATTTTCAAATCCATACAGTATTGAGTATTCTGAACTTGACTGT GAGGAAGGGTGGACACAACTGAAGTGTGGAAGAAATGAAAGGGCGAAGGTGTGTTTTGAGAAGGCTCTGG AAGAAAAGCCCAACAACCCAGAATTCTCCTCTGGACTGGCAATTGCGATGTACCATCTGGATAATCACCC AGAGAAACAGTTCTCTACTGATGTTTTGAAGCAGGCCATTGAGCTGAGTCCTGATAACCAATACGTCAAG GTTCTCTTGGGCCTGAAACTGCAGAAGATGAATAAAGAAGCTGAAGGAGAGCAGTTTGTTGAAGAAGCCT TGGAAAAGTCTCCTTGCCAAACAGATGTCCTCCGCAGTGCAGCCAAATTTTACAGAAGAAAAGGTGACCT AGACAAAGCTATTGAACTGTTTCAACGGGTGTTGGAATCCACACCAAACAATGGCTACCTCTATCACCAG ATTGGGTGCTGCTACAAGGCAAAAGTAAGACAAATGCAGAATACAGGAGAATCTGAAGCTAGTGGAAATA AAGAGATGATTGAAGCACTAAAGCAATATGCTATGGACTATTCGAATAAAGCTCTTGAGAAGGGACTGAA TCCTCTGAATGCATACTCCGATCTCGCTGAGTTCCTGGAGACGGAATGTTATCAGACACCATTCAATAAG GAAGTCCCTGATGCTGAAAAGCAACAATCCCATCAGCGCTACTGCAACCTTCAGAAATATAATGGGAAGT CTGAAGACACTGCTGTGCAACATGGTTTAGAGGGTTTGTCCATAAGCAAAAAATCAACTGACAAGGAAGA GATCAAAGACCAACCACAGAATGTATCTGAAAATCTGCTTCCACAAAATGCACCAAATTATTGGTATCTT CAAGGATTAATTCATAAGCAGAATGGAGATCTGCTGCAAGCAGCCAAATGTTATGAGAAGGAACTGGGCC GCCTGCTAAGGGATGCCCCTTCAGGCATAGGCAGTATTTTCCTGTCAGCATCTGAGCTTGAGGATGGTAG TGAGGAAATGGGCCAGGGCGCAGTCAGCTCCAGTCCCAGAGAGCTCCTCTCTAACTCAGAGCAACTGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214701 protein sequence
Red=Cloning site Green=Tags(s) MSEVTKNSLEKILPQLKCHFTWNLFKEDSVSRDLEDRVCNQIEFLNTEFKATMYNLLAYIKHLDGNNEAA LECLRQAEELIQQEHADQAEIRSLVTWGNYAWVYYHLGRLSDAQIYVDKVKQTCKKFSNPYSIEYSELDC EEGWTQLKCGRNERAKVCFEKALEEKPNNPEFSSGLAIAMYHLDNHPEKQFSTDVLKQAIELSPDNQYVK VLLGLKLQKMNKEAEGEQFVEEALEKSPCQTDVLRSAAKFYRRKGDLDKAIELFQRVLESTPNNGYLYHQ IGCCYKAKVRQMQNTGESEASGNKEMIEALKQYAMDYSNKALEKGLNPLNAYSDLAEFLETECYQTPFNK EVPDAEKQQSHQRYCNLQKYNGKSEDTAVQHGLEGLSISKKSTDKEEIKDQPQNVSENLLPQNAPNYWYL QGLIHKQNGDLLQAAKCYEKELGRLLRDAPSGIGSIFLSASELEDGSEEMGQGAVSSSPRELLSNSEQLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001031683 |
| ORF Size | 1470 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001031683.4 |
| RefSeq Size | 2467 bp |
| RefSeq ORF | 1473 bp |
| Locus ID | 3437 |
| UniProt ID | O14879 |
| MW | 56 kDa |
| Gene Summary | IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exihibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin-dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Upregulates CDKN1A/p21 by downregulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214701L3 | Lenti ORF clone of Human interferon-induced protein with tetratricopeptide repeats 3 (IFIT3), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC214701L4 | Lenti ORF clone of Human interferon-induced protein with tetratricopeptide repeats 3 (IFIT3), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG214701 | IFIT3 (tGFP-tagged) - Human interferon-induced protein with tetratricopeptide repeats 3 (IFIT3), transcript variant 2 |
CNY 5,256.00 |
|
| SC302530 | IFIT3 (untagged)-Human interferon-induced protein with tetratricopeptide repeats 3 (IFIT3), transcript variant 2 |
CNY 3,656.00 |
