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p114RhoGEF (ARHGEF18) (NM_015318) Human Tagged ORF Clone
CAT＃: RC213956
- TrueORF®
ARHGEF18 (Myc-DDK-tagged)-Human Rho/Rac guanine nucleotide exchange factor (GEF) 18 (ARHGEF18), transcript variant 1
ORF Plasmid: tGFP
"NM_015318" in other vectors (4)
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CNY 10,170.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213956 representing NM_015318
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGTCTCTCAGAAAGGGGGTCCCCAGCCAACACCGAGCCCGGCTGGCCCTGGGACGCAACTCGGAC CAATCACAGGAGAGATGGATGAAGCCGATTCTGCGTTTTTAAAATTTAAGCAGACAGCTGATGACTCTCT GTCCCTTACATCTCCAAACACCGAGTCCATTTTTGTAGAAGATCCCTACACCGCCTCGCTGAGGAGTGAG ATTGAGTCAGACGGCCACGAGTTTGAAGCTGAGTCCTGGAGCCTCGCCGTGGATGCAGCCTACGCCAAGA AGCAAAAGAGGGAGGTGGTGAAAAGACAAGATGTCCTTTATGAGCTGATGCAGACAGAGGTGCACCACGT GCGGACGCTCAAGATCATGCTGAAGGTGTACTCCAGGGCCCTGCAGGAGGAGCTGCAGTTCAGCAGCAAG GCCATTGGCCGCCTCTTCCCATGCGCTGACGACCTGCTGGAGACGCACAGCCACTTCCTCGCTCGGCTCA AGGAGCGCCGCCAGGAGTCCCTGGAGGAGGGCAGTGACCGGAATTATGTCATCCAGAAAATCGGCGACCT CCTGGTTCAGCAGTTTTCAGGTGAAAATGGGGAGAGAATGAAAGAAAAGTACGGTGTGTTTTGTAGTGGC CACAATGAAGCTGTTAGTCATTACAAGTTGCTGCTTCAGCAAAACAAGAAATTTCAAAACTTGATCAAGA AAATTGGCAACTTCTCCATCGTGCGGCGGCTTGGCGTGCAGGAGTGCATTCTCCTGGTTACACAACGCAT AACCAAATACCCAGTGCTGGTGGAGCGCATCATCCAGAACACGGAAGCTGGCACTGAGGACTATGAAGAC CTGACCCAGGCCTTGAACCTCATCAAAGATATCATCTCACAAGTGGACGCCAAGGTCAGTGAGTGTGAGA AGGGCCAGCGCCTCAGGGAGATCGCAGGGAAGATGGACCTGAAGTCTTCCAGCAAACTCAAGAACGGGCT CACCTTCCGCAAGGAAGACATGCTTCAGCGGCAGCTCCACCTGGAGGGCATGCTATGCTGGAAGACCACA TCAGGGCGCTTGAAAGATATCCTGGCTATCCTGCTGACCGACGTACTTTTGCTGCTACAAGAAAAAGATC AGAAATACGTCTTTGCTTCTGTGGACTCAAAGCCACCCGTCATCTCGTTACAAAAGCTCATCGTGAGGGA AGTGGCCAACGAGGAGAAAGCGATGTTTCTGATCAGCGCCTCCTTGCAAGGGCCGGAGATGTATGAAATC TACACGAGCTCCAAAGAGGACAGGAACGCCTGGATGGCCCACATCCAAAGGGCTGTGGAGAGCTGCCCTG ACGAGGAGGAGGGGCCCTTCAGCCTGCCCGAAGAGGAAAGGAAGGTGGTCGAGGCCCGCGCCACGAGACT CCGGGACTTTCAAGAGCGGTTGAGCATGAAAGACCAGCTGATCGCACAGAGCCTCCTAGAGAAACAGCAG ATCTACCTGGAGATGGCCGAGATGGGCGGCCTCGAAGACCTGCCCCAGCCCCGAGGCCTATTCCGTGGAG GGGACCCATCCGAGACCCTGCAGGGGGAGCTAATTCTCAAGTCGGCCATGAGCGAGATCGAGGGCATCCA GAGCCTGATCTGCAGGCAGCTGGGCAGCGCCAACGGCCAGGCGGAAGACGGAGGCAGCTCCACAGGCCCG CCCAGGAGGGCTGAGACCTTCGCGGGCTACGACTGCACAAACAGCCCCACCAAGAATGGCAGTTTCAAGA AGAAAGTCAGCAGCACTGACCCCAGGCCCCGAGACTGGCGAGGCCCCCCAAACAGCCCGGACTTGAAGCT CAGTGACAGTGACATTCCTGGGAGCTCTGAGGAATCGCCGCAGGTGGTGGAGGCGCCAGGCACGGAATCC GATCCCCGTCTGCCCACCGTCCTGGAGTCGGAGCTTGTCCAGCGGATCCAGACACTGTCCCAGCTGCTCC TGAACCTTCAGGCGGTAATCGCCCACCAGGACAGCTATGTGGAGACGCAGCGGGCTGCCATCCAGGAGCG GGAGAAGCAGTTCCGGCTGCAGTCGACGCGTGGGAACCTGCTGCTGGAGCAGGAGCGGCAACGCAACTTC GAGAAGCAGCGGGAGGAGCGCGCGGCCCTGGAGAAGCTGCAGAGCCAGCTGCGGCACGAGCAGCAGCGCT GGGAGCGCGAGCGCCAGTGGCAGCACCAGGAGCTGGAGCGTGCGGGCGCGCGGCTGCAGGAGCGCGAGGG CGAGGCGCGGCAGCTACGCGAGCGGCTGGAGCAGGAGCGGGCCGAGCTGGAGCGCCAGCGCCAGGCCTAC CAGCACGACCTGGAGCGGCTGCGCGAGGCCCAGCGTGCCGTGGAGCGCGAGCGGGAGCGCCTGGAGCTGC TGCGCCGCCTCAAGAAGCAGAACACCGCGCCAGGCGCGCTGCCGCCCGACACACTGGCCGAGGCCCAGCC CCCAAGCCACCCTCCCAGCTTCAACGGGGAAGGGCTGGAGGGCCCTCGTGTGAGCATGCTGCCATCCGGC GTGGGGCCAGAGTACGCAGAGCGCCCCGAGGTGGCTCGCCGGGACAGCGCCCCCACCGAGAACCGGCTGG CCAAGAGCGATGTGCCCATCCAGCTGCTCAGCGCCACCAACCAGTTCCAGAGGCAGGCGGCCGTGCAGCA GCAGATCCCCACCAAGCTGGCGGCCTCCACCAAGGGTGGCAAGGACAAGGGCGGCAAGAGCAGGGGCTCT CAGCGCTGGGAGAGCTCAGCGTCCTTCGACCTGAAGCAGCAGCTGCTGCTCAACAAGCTCATGGGGAAAG ATGAGAGCACCTCACGGAACCGCCGCTCGCTGAGCCCTATCCTGCCCGGCAGACACAGTCCTGCGCCCCC ACCAGACCCTGGCTTCCCCGCCCCGAGCCCACCGCCAGCTGACAGCCCCTCCGAGGGCTTCTCTCTCAAG GCCGGGGGCACAGCCCTCCTGCCCGGGCCCCCAGCTCCCTCGCCACTGCCGGCCACACCACTCAGCGCCA AGGAGGACGCCAGCAAAGAAGACGTCATCTTCTTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC213956 representing NM_015318
Red=Cloning site Green=Tags(s) MTVSQKGGPQPTPSPAGPGTQLGPITGEMDEADSAFLKFKQTADDSLSLTSPNTESIFVEDPYTASLRSE IESDGHEFEAESWSLAVDAAYAKKQKREVVKRQDVLYELMQTEVHHVRTLKIMLKVYSRALQEELQFSSK AIGRLFPCADDLLETHSHFLARLKERRQESLEEGSDRNYVIQKIGDLLVQQFSGENGERMKEKYGVFCSG HNEAVSHYKLLLQQNKKFQNLIKKIGNFSIVRRLGVQECILLVTQRITKYPVLVERIIQNTEAGTEDYED LTQALNLIKDIISQVDAKVSECEKGQRLREIAGKMDLKSSSKLKNGLTFRKEDMLQRQLHLEGMLCWKTT SGRLKDILAILLTDVLLLLQEKDQKYVFASVDSKPPVISLQKLIVREVANEEKAMFLISASLQGPEMYEI YTSSKEDRNAWMAHIQRAVESCPDEEEGPFSLPEEERKVVEARATRLRDFQERLSMKDQLIAQSLLEKQQ IYLEMAEMGGLEDLPQPRGLFRGGDPSETLQGELILKSAMSEIEGIQSLICRQLGSANGQAEDGGSSTGP PRRAETFAGYDCTNSPTKNGSFKKKVSSTDPRPRDWRGPPNSPDLKLSDSDIPGSSEESPQVVEAPGTES DPRLPTVLESELVQRIQTLSQLLLNLQAVIAHQDSYVETQRAAIQEREKQFRLQSTRGNLLLEQERQRNF EKQREERAALEKLQSQLRHEQQRWERERQWQHQELERAGARLQEREGEARQLRERLEQERAELERQRQAY QHDLERLREAQRAVERERERLELLRRLKKQNTAPGALPPDTLAEAQPPSHPPSFNGEGLEGPRVSMLPSG VGPEYAERPEVARRDSAPTENRLAKSDVPIQLLSATNQFQRQAAVQQQIPTKLAASTKGGKDKGGKSRGS QRWESSASFDLKQQLLLNKLMGKDESTSRNRRSLSPILPGRHSPAPPPDPGFPAPSPPPADSPSEGFSLK AGGTALLPGPPAPSPLPATPLSAKEDASKEDVIFF SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015318 |
| ORF Size | 3045 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015318.4 |
| RefSeq Size | 5252 bp |
| RefSeq ORF | 3048 bp |
| Locus ID | 23370 |
| UniProt ID | Q6ZSZ5 |
| Domains | RhoGEF, PH |
| MW | 114.1 kDa |
| Gene Summary | Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GEF family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Nov 2018] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213956L3 | Lenti-ORF clone of ARHGEF18 (Myc-DDK-tagged)-Human Rho/Rac guanine nucleotide exchange factor (GEF) 18 (ARHGEF18), transcript variant 1 |
CNY 12,070.00 |
|
| RC213956L4 | Lenti-ORF clone of ARHGEF18 (mGFP-tagged)-Human Rho/Rac guanine nucleotide exchange factor (GEF) 18 (ARHGEF18), transcript variant 1 |
CNY 12,070.00 |
|
| RG213956 | ARHGEF18 (tGFP-tagged) - Human Rho/Rac guanine nucleotide exchange factor (GEF) 18 (ARHGEF18), transcript variant 1 |
CNY 11,210.00 |
|
| SC308747 | ARHGEF18 (untagged)-Human Rho/Rac guanine nucleotide exchange factor (GEF) 18 (ARHGEF18), transcript variant 1 |
CNY 7,072.00 |
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