p114RhoGEF (ARHGEF18) (NM_015318) Human Untagged Clone

CAT#: SC308747

ARHGEF18 (untagged)-Human Rho/Rac guanine nucleotide exchange factor (GEF) 18 (ARHGEF18), transcript variant 1



  "NM_015318" in other vectors (4)

CNY 7,072.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
ARHGEF18 mouse monoclonal antibody,clone OTI6D10
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF
Vector PCMV6-Neo
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_015318 edited
ATGACGGTCTCTCAGAAAGGGGGTCCCCAGCCAACACCGAGCCCGGCTGGCCCTGGGACG
CAACTCGGACCAATCACAGGAGAGATGGATGAAGCCGATTCTGCGTTTTTAAAATTTAAG
CAGACAGCTGATGACTCTCTGTCCCTTACATCTCCAAACACCGAGTCCATTTTTGTAGAA
GATCCCTACACCGCCTCGCTGAGGAGTGAGATTGAGTCAGACGGCCACGAGTTTGAAGCT
GAGTCCTGGAGCCTCGCCGTGGATGCAGCCTACGCCAAGAAGCAAAAGAGGGAGGTGGTG
AAAAGACAAGATGTCCTTTATGAGCTGATGCAGACAGAGGTGCACCACGTGCGGACGCTC
AAGATCATGCTGAAGGTGTACTCCAGGGCCCTGCAGGAGGAGCTGCAGTTCAGCAGCAAG
GCCATTGGCCGCCTCTTCCCATGCGCTGACGACCTGCTGGAGACGCACAGCCACTTCCTC
GCTCGGCTCAAGGAGCGCCGCCAGGAGTCCCTGGAGGAGGGCAGTGACCGGAATTATGTC
ATCCAGAAAATCGGCGACCTCCTGGTTCAGCAGTTTTCAGGTGAAAATGGGGAGAGAATG
AAAGAAAAGTACGGTGTGTTTTGTAGTGGCCACAATGAAGCTGTTAGTCATTACAAGTTG
CTGCTTCAGCAAAACAAGAAATTTCAAAACTTGATCAAGAAAATTGGCAACTTCTCCATC
GTGCGGCGGCTTGGCGTGCAGGAGTGCATTCTCCTGGTTACACAACGCATAACCAAATAC
CCAGTGCTGGTGGAGCGCATCATCCAGAACACGGAAGCTGGCACTGAGGACTATGAAGAC
CTGACCCAGGCCTTGAACCTCATCAAAGATATCATCTCACAAGTGGACGCCAAGGTCAGT
GAGTGTGAGAAGGGCCAGCGCCTCAGGGAGATCGCAGGGAAGATGGACCTGAAGTCTTCC
AGCAAACTCAAGAACGGGCTCACCTTCCGCAAGGAAGACATGCTTCAGCGGCAGCTCCAC
CTGGAGGGCATGCTATGCTGGAAGACCACATCAGGGCGCTTGAAAGATATCCTGGCTATC
CTGCTGACCGACGTACTTTTGCTGCTACAAGAAAAAGATCAGAAATACGTCTTTGCTTCT
GTGGACTCAAAGCCACCCGTCATCTCGTTACAAAAGCTCATCGTGAGGGAAGTGGCCAAC
GAGGAGAAAGCGATGTTTCTGATCAGCGCCTCCTTGCAAGGGCCGGAGATGTATGAAATC
TACACGAGCTCCAAAGAGGACAGGAACGCCTGGATGGCCCACATCCAAAGGGCTGTGGAG
AGCTGCCCTGACGAGGAGGAGGGGCCCTTCAGCCTGCCCGAAGAGGAAAGGAAGGTGGTC
GAGGCCCGCGCCACGAGACTCCGGGACTTTCAAGAGCGGTTGAGCATGAAAGACCAGCTG
ATCGCACAGAGCCTCCTAGAGAAACAGCAGATCTACCTGGAGATGGCCGAGATGGGCGGC
CTCGAAGACCTGCCCCAGCCCCGAGGCCTATTCCGTGGAGGGGACCCATCCGAGACCCTG
CAGGGGGAGCTAATTCTCAAGTCGGCCATGAGCGAGATCGAGGGCATCCAGAGCCTGATC
TGCAGGCAGCTGGGCAGCGCCAACGGCCAGGCGGAAGACGGAGGCAGCTCCACAGGCCCG
CCCAGGAGGGCTGAGACCTTCGCGGGCTACGACTGCACAAACAGCCCCACCAAGAATGGC
AGTTTCAAGAAGAAAGTCAGCAGCACTGACCCCAGGCCCCGAGACTGGCGAGGCCCCCCA
AACAGCCCGGACTTGAAGCTCAGTGACAGTGACATTCCTGGGAGCTCTGAGGAATCGCCG
CAGGTGGTGGAGGCGCCAGGCACGGAATCCGATCCCCGTCTGCCCACCGTCCTGGAGTCG
GAGCTTGTCCAGCGGATCCAGACACTGTCCCAGCTGCTCCTGAACCTTCAGGCGGTAATC
GCCCACCAGGACAGCTATGTGGAGACGCAGCGGGCTGCCATCCAGGAGCGGGAGAAGCAG
TTCCGGCTGCAGTCGACGCGTGGGAACCTGCTGCTGGAGCAGGAGCGGCAACGCAACTTC
GAGAAGCAGCGGGAGGAGCGCGCGGCCCTGGAGAAGCTGCAGAGCCAGCTGCGGCACGAG
CAGCAGCGCTGGGAGCGCGAGCGCCAGTGGCAGCACCAGGAGCTGGAGCGTGCGGGCGCG
CGGCTGCAGGAGCGCGAGGGCGAGGCGCGGCAGCTACGCGAGCGGCTGGAGCAGGAGCGG
GCCGAGCTGGAGCGCCAGCGCCAGGCCTACCAGCACGACCTGGAGCGGCTGCGCGAGGCC
CAGCGTGCCGTGGAGCGCGAGCGGGAGCGCCTGGAGCTGCTGCGCCGCCTCAAGAAGCAG
AACACCGCGCCAGGCGCGCTGCCGCCCGACACACTGGCCGAGGCCCAGCCCCCAAGCCAC
CCTCCCAGCTTCAACGGGGAAGGGCTGGAGGGCCCTCGTGTGAGCATGCTGCCATCCGGC
GTGGGGCCAGAGTACGCAGAGCGCCCCGAGGTGGCTCGCCGGGACAGCGCCCCCACCGAG
AACCGGCTGGCCAAGAGCGATGTGCCCATCCAGCTGCTCAGCGCCACCAACCAGTTCCAG
AGGCAGGCGGCCGTGCAGCAGCAGATCCCCACCAAGCTGGCGGCCTCCACCAAGGGTGGC
AAGGACAAGGGCGGCAAGAGCAGGGGCTCTCAGCGCTGGGAGAGCTCAGCGTCCTTCGAC
CTGAAGCAGCAGCTGCTGCTCAACAAGCTCATGGGGAAAGATGAGAGCACCTCACGGAAC
CGCCGCTCGCTGAGCCCTATCCTGCCCGGCAGACACAGTCCTGCGCCCCCACCAGACCCT
GGCTTCCCCGCCCCGAGCCCACCGCCAGCTGACAGCCCCTCCGAGGGCTTCTCTCTCAAG
GCCGGGGGCACAGCCCTCCTGCCCGGGCCCCCAGCTCCCTCGCCACTGCCGGCCACACCA
CTCAGCGCCAAGGAGGACGCCAGCAAAGAAGACGTCATCTTCTTCTAA
Restriction Sites Please inquire     
ACCN NM_015318
Insert Size 3000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_015318.2, NP_056133.2
RefSeq Size 5386 bp
RefSeq ORF 3048 bp
Locus ID 23370
UniProt ID Q6ZSZ5
Domains RhoGEF, PH
Gene Summary Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GEF family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Nov 2018]
Transcript Variant: This variant (1) represents the shorter transcript, and encodes the shorter isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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