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SF3B3 (NM_012426) Human Tagged ORF Clone
CAT＃: RC209509
SF3B3 (Myc-DDK-tagged)-Human splicing factor 3b, subunit 3, 130kDa (SF3B3)
ORF Plasmid: tGFP
"NM_012426" in other vectors (7)
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CNY 8,472.00
| Cited in 1 publication. |
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RSE1; SAP130; SF3b130; STAF130 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209509 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCTGTACAACTTAACCTTGCAGAGAGCCACTGGCATCAGCTTTGCCATTCATGGAAACTTTTCTG GAACCAAACAACAAGAAATTGTTGTTTCCCGTGGGAAGATCTTGGAGCTGCTTCGCCCAGACCCCAACAC TGGCAAAGTACATACCCTACTCACTGTGGAAGTATTCGGTGTTATCCGGTCACTCATGGCCTTTAGGCTG ACAGGTGGCACCAAAGACTACATTGTAGTTGGCAGTGACTCTGGTCGAATTGTTATTTTGGAATACCAGC CATCTAAGAATATGTTTGAGAAGATTCACCAAGAAACCTTTGGCAAGAGTGGATGCCGTCGCATCGTTCC TGGCCAGTTCTTAGCTGTGGATCCCAAAGGGCGAGCCGTTATGATTAGTGCCATTGAGAAACAGAAATTG GTGTATATTTTGAACAGAGATGCTGCAGCCCGACTTACCATTTCATCTCCCCTGGAAGCCCACAAAGCAA ACACTTTAGTGTATCATGTAGTTGGAGTAGATGTCGGATTTGAAAATCCAATGTTTGCTTGTCTGGAAAT GGATTATGAGGAAGCAGACAATGATCCAACAGGGGAAGCAGCAGCTAATACCCAGCAGACACTTACTTTC TATGAGCTAGACCTTGGTTTAAATCATGTGGTCCGAAAATACAGTGAACCTTTGGAGGAACACGGCAACT TCCTTATTACAGTTCCAGGAGGGTCAGATGGTCCAAGTGGAGTACTGATCTGCTCTGAAAACTATATTAC TTACAAGAACTTTGGTGACCAGCCAGATATCCGCTGTCCAATTCCCAGGAGGCGGAATGACCTGGATGAC CCTGAAAGAGGAATGATTTTTGTCTGCTCTGCAACCCATAAAACCAAATCGATGTTCTTCTCTTTGGCTC AAACTGAGCAGGGAGATATCTTTAAGATCACTTTGGAGACAGATGAAGATATGGTTACTGAGATCCGGCT CAAATATTTTGATACTGTACCCGTTGCTGCTGCCATGTGTGTGCTTAAAACAGGGTTCCTTTTTGTAGCA TCAGAATTTGGAAACCATTACTTATATCAAATTGCACATCTTGGAGATGATGATGAAGAACCTGAGTTTT CATCAGCCATGCCTCTGGAAGAAGGAGACACATTCTTTTTTCAGCCAAGACCACTTAAAAACCTTGTGCT GGTTGATGAGTTGGACAGCCTCTCTCCCATTCTGTTTTGCCAGATAGCTGATCTGGCCAATGAAGATACT CCACAGTTGTATGTGGCCTGTGGTAGGGGACCCCGATCATCTCTGAGAGTCCTAAGACATGGACTTGAGG TGTCAGAAATGGCTGTTTCTGAGCTACCTGGTAACCCCAACGCTGTCTGGACAGTGCGTCGACACATTGA AGATGAGTTTGATGCCTACATTATTGTGTCTTTCGTGAATGCCACCCTAGTGTTGTCCATTGGAGAAACT GTAGAAGAAGTGACTGACTCTGGGTTCCTGGGGACCACCCCGACCTTGTCCTGCTCCTTATTAGGAGATG ATGCCTTGGTGCAGGTCTATCCAGATGGCATTCGGCACATACGAGCAGACAAGAGAGTCAATGAGTGGAA GACCCCTGGAAAGAAAACAATTGTGAAGTGTGCAGTGAACCAGCGACAAGTGGTGATTGCCCTGACAGGA GGAGAGCTGGTCTATTTCGAGATGGATCCTTCAGGACAGCTGAATGAGTACACAGAACGGAAGGAGATGT CAGCAGATGTGGTGTGCATGAGTCTGGCCAATGTACCCCCTGGAGAGCAGCGGTCTCGCTTCCTGGCTGT GGGGCTTGTGGACAACACTGTCAGAATCATCTCCCTGGATCCCTCAGACTGTTTGCAACCTCTAAGCATG CAGGCTCTCCCAGCCCAGCCTGAGTCCTTGTGTATCGTGGAAATGGGTGGGACTGAGAAGCAGGATGAGC TGGGTGAGAGGGGCTCGATTGGCTTCCTATACCTGAATATTGGGCTACAGAACGGTGTGCTGCTGAGGAC TGTCTTGGACCCTGTCACTGGGGATTTGTCTGATACTCGCACTCGGTACCTGGGGTCCCGTCCTGTGAAG CTCTTCCGAGTCCGAATGCAAGGCCAGGAGGCAGTATTGGCCATGTCAAGCCGCTCATGGTTGAGCTATT CTTACCAATCTCGCTTCCATCTCACCCCACTGTCTTACGAGACACTGGAATTTGCATCGGGTTTTGCCTC GGAACAGTGTCCCGAGGGCATTGTGGCCATCTCCACCAACACCCTACGGATTTTGGCATTAGAGAAGCTC GGTGCTGTCTTCAATCAAGTAGCCTTCCCACTGCAGTACACACCCAGGAAATTTGTCATCCACCCTGAGA GTAACAACCTTATTATCATTGAAACGGACCACAATGCCTACACTGAGGCCACGAAAGCTCAGAGAAAGCA GCAGATGGCAGAGGAAATGGTGGAAGCAGCAGGGGAGGATGAGCGGGAGCTGGCCGCAGAGATGGCAGCA GCATTCCTCAATGAAAACCTCCCTGAATCCATCTTTGGAGCTCCCAAGGCTGGCAATGGGCAGTGGGCCT CTGTGATCCGAGTGATGAATCCCATTCAAGGGAACACACTGGACCTTGTCCAGCTGGAACAGAATGAGGC AGCTTTTAGTGTGGCTGTGTGCAGGTTTTCCAACACTGGTGAAGACTGGTATGTGCTGGTGGGTGTGGCC AAGGACCTGATACTAAACCCCCGATCTGTGGCAGGGGGCTTCGTCTATACTTACAAGCTTGTGAACAATG GGGAAAAACTGGAGTTTTTGCACAAGACTCCTGTGGAAGAGGTCCCTGCTGCTATTGCCCCATTCCAGGG GAGGGTGTTGATTGGTGTGGGGAAGCTGTTGCGTGTCTATGACCTGGGAAAGAAGAAGTTACTCCGAAAA TGTGAGAATAAGCATATTGCCAATTATATCTCTGGGATCCAGACTATCGGACATAGGGTAATTGTATCTG ATGTCCAAGAAAGTTTCATCTGGGTTCGCTACAAGCGTAATGAAAACCAGCTTATCATCTTTGCTGATGA TACCTACCCCCGATGGGTCACTACAGCCAGCCTCCTGGACTATGACACTGTGGCTGGGGCAGACAAGTTT GGCAACATATGTGTGGTGAGGCTCCCACCTAACACCAATGATGAAGTAGATGAGGATCCTACAGGAAACA AAGCCCTGTGGGACCGTGGCTTGCTCAATGGGGCCTCCCAGAAGGCAGAGGTGATCATGAACTACCATGT CGGGGAGACGGTGCTGTCCTTGCAGAAGACCACGCTGATCCCTGGAGGCTCAGAATCACTTGTCTATACC ACCTTGTCTGGAGGAATTGGCATCCTTGTGCCATTCACGTCCCATGAGGACCATGACTTCTTCCAGCATG TGGAAATGCACCTGCGGTCTGAACATCCCCCTCTCTGTGGGCGGGACCACCTCAGCTTTCGCTCCTACTA CTTCCCTGTGAAGAATGTGATTGATGGAGACCTCTGTGAGCAGTTCAATTCCATGGAACCCAACAAACAA AAGAACGTCTCTGAAGAACTGGACCGAACCCCACCCGAAGTGTCCAAGAAACTCGAGGATATCCGGACCC GCTACGCCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209509 protein sequence
Red=Cloning site Green=Tags(s) MFLYNLTLQRATGISFAIHGNFSGTKQQEIVVSRGKILELLRPDPNTGKVHTLLTVEVFGVIRSLMAFRL TGGTKDYIVVGSDSGRIVILEYQPSKNMFEKIHQETFGKSGCRRIVPGQFLAVDPKGRAVMISAIEKQKL VYILNRDAAARLTISSPLEAHKANTLVYHVVGVDVGFENPMFACLEMDYEEADNDPTGEAAANTQQTLTF YELDLGLNHVVRKYSEPLEEHGNFLITVPGGSDGPSGVLICSENYITYKNFGDQPDIRCPIPRRRNDLDD PERGMIFVCSATHKTKSMFFSLAQTEQGDIFKITLETDEDMVTEIRLKYFDTVPVAAAMCVLKTGFLFVA SEFGNHYLYQIAHLGDDDEEPEFSSAMPLEEGDTFFFQPRPLKNLVLVDELDSLSPILFCQIADLANEDT PQLYVACGRGPRSSLRVLRHGLEVSEMAVSELPGNPNAVWTVRRHIEDEFDAYIIVSFVNATLVLSIGET VEEVTDSGFLGTTPTLSCSLLGDDALVQVYPDGIRHIRADKRVNEWKTPGKKTIVKCAVNQRQVVIALTG GELVYFEMDPSGQLNEYTERKEMSADVVCMSLANVPPGEQRSRFLAVGLVDNTVRIISLDPSDCLQPLSM QALPAQPESLCIVEMGGTEKQDELGERGSIGFLYLNIGLQNGVLLRTVLDPVTGDLSDTRTRYLGSRPVK LFRVRMQGQEAVLAMSSRSWLSYSYQSRFHLTPLSYETLEFASGFASEQCPEGIVAISTNTLRILALEKL GAVFNQVAFPLQYTPRKFVIHPESNNLIIIETDHNAYTEATKAQRKQQMAEEMVEAAGEDERELAAEMAA AFLNENLPESIFGAPKAGNGQWASVIRVMNPIQGNTLDLVQLEQNEAAFSVAVCRFSNTGEDWYVLVGVA KDLILNPRSVAGGFVYTYKLVNNGEKLEFLHKTPVEEVPAAIAPFQGRVLIGVGKLLRVYDLGKKKLLRK CENKHIANYISGIQTIGHRVIVSDVQESFIWVRYKRNENQLIIFADDTYPRWVTTASLLDYDTVAGADKF GNICVVRLPPNTNDEVDEDPTGNKALWDRGLLNGASQKAEVIMNYHVGETVLSLQKTTLIPGGSESLVYT TLSGGIGILVPFTSHEDHDFFQHVEMHLRSEHPPLCGRDHLSFRSYYFPVKNVIDGDLCEQFNSMEPNKQ KNVSEELDRTPPEVSKKLEDIRTRYAF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012426 |
| ORF Size | 3651 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq Size | 9736 bp |
| RefSeq ORF | 3654 bp |
| Locus ID | 23450 |
| UniProt ID | Q15393 |
| Domains | CPSF_A |
| Protein Pathways | Spliceosome |
| MW | 135.5 kDa |
| Gene Summary | This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Jerantinine A induces tumor-specific cell death through modulation of splicing factor 3b subunit 1 (SF3B1)
,Chung, FF;Tan, PF;Raja, VJ;Tan, BS;Lim, KH;Kam, TS;Hii, LW;Tan, SH;See, SJ;Tan, YF;Wong, LZ;Yam, WK;Mai, CW;Bradshaw, TD;Leong, CO;,
Sci Rep
,PubMed ID 28198434
[SF3B3]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209509L1 | Lenti ORF clone of Human splicing factor 3b, subunit 3, 130kDa (SF3B3), Myc-DDK-tagged |
CNY 10,872.00 |
|
| RC209509L2 | Lenti ORF clone of Human splicing factor 3b, subunit 3, 130kDa (SF3B3), mGFP tagged |
CNY 14,060.00 |
|
| RC209509L3 | Lenti ORF clone of Human splicing factor 3b, subunit 3, 130kDa (SF3B3), Myc-DDK-tagged |
CNY 14,060.00 |
|
| RC209509L4 | Lenti ORF clone of Human splicing factor 3b, subunit 3, 130kDa (SF3B3), mGFP tagged |
CNY 14,060.00 |
|
| RG209509 | SF3B3 (tGFP-tagged) - Human splicing factor 3b, subunit 3, 130kDa (SF3B3) |
CNY 10,072.00 |
|
| SC126731 | SF3B3 (untagged)-Human splicing factor 3b, subunit 3, 130kDa (SF3B3) |
CNY 8,480.00 |
|
| SC323872 | SF3B3 (untagged)-Human splicing factor 3b, subunit 3, 130kDa (SF3B3) |
CNY 8,480.00 |
