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SF3B3 (NM_012426) Human Untagged Clone
CAT＃: SC126731
SF3B3 (untagged)-Human splicing factor 3b, subunit 3, 130kDa (SF3B3)
CNY 8,480.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | RSE1; SAP130; SF3b130; STAF130 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC126731 sequence for NM_012426 edited (data generated by NextGen Sequencing)
ATGTTTCTGTACAACTTAACCTTGCAGAGAGCCACTGGCATCAGCTTTGCCATTCATGGA AACTTTTCTGGAACCAAACAACAAGAAATTGTTGTTTCCCGTGGGAAGATCTTGGAGCTG CTTCGCCCAGACCCCAACACTGGCAAAGTACATACCCTACTCACTGTGGAAGTATTCGGT GTTATCCGGTCACTCATGGCCTTTAGGCTGACAGGTGGCACCAAAGACTACATTGTAGTT GGCAGTGACTCTGGTCGAATTGTTATTTTGGAATACCAGCCATCTAAGAATATGTTTGAG AAGATTCACCAAGAAACCTTTGGCAAGAGTGGATGCCGTCGCATCGTTCCTGGCCAGTTC TTAGCTGTGGATCCCAAAGGGCGAGCCGTTATGATTAGTGCCATTGAGAAACAGAAATTG GTGTATATTTTGAACAGAGATGCTGCAGCCCGACTTACCATTTCATCTCCCCTGGAAGCC CACAAAGCAAACACTTTAGTGTATCATGTAGTTGGAGTAGATGTCGGATTTGAAAATCCA ATGTTTGCTTGTCTGGAAATGGATTATGAGGAAGCAGACAATGATCCAACAGGGGAAGCA GCAGCTAATACCCAGCAGACACTTACTTTCTATGAGCTAGACCTTGGTTTAAATCATGTG GTCCGAAAATACAGTGAACCTTTGGAGGAACACGGCAACTTCCTTATTACAGTTCCTGGA GGGTCAGATGGTCCAAGTGGAGTACTGATCTGCTCTGAAAACTATATTACTTACAAGAAC TTTGGTGACCAGCCAGATATCCGCTGTCCAATTCCCAGGAGGCGGAATGACCTGGATGAC CCTGAAAGAGGAATGATTTTTGTCTGCTCTGCAACCCATAAAACCAAATCGATGTTCTTC TTTTTGGCTCAAACTGAGCAGGGAGATATCTTTAAGATCACTTTGGAGACAGATGAAGAT ATGGTTACTGAGATCCGGCTCAAATATTTTGATACTGTACCCGTTGCTGCTGCCATGTGT GTGCTTAAAACAGGGTTCCTTTTTGTAGCATCAGAATTTGGAAACCATTACTTATATCAA ATTGCACATCTTGGAGATGATGATGAAGAACCTGAGTTTTCATCAGCCATGCCTCTGGAA GAAGGAGACACATTCTTTTTTCAGCCAAGACCACTTAAAAACCTTGTGCTGGTTGATGAG TTGGACAGCCTCTCTCCCATTCTGTTTTGCCAGATAGCTGATCTGGCCAATGAAGATACT CCACAGTTGTATGTGGCCTGTGGTAGGGGACCCCGATCATCTCTGAGAGTCCTAAGACAT GGACTTGAGGTGTCAGAAATGGCTGTTTCTGAGCTACCTGGTAACCCCAACGCTGTCTGG ACAGTGCGTCGACACATTGAAGATGAGTTTGATGCCTACATCATTGTGTCTTTCGTGAAT GCCACCCTAGTGTTGTCCATTGGAGAAACTGTAGAAGAAGTGACTGACTCTGGGTTCCTG GGGACCACCCCGACCTTGTCCTGCTCCTTATTAGGAGATGATGCCTTGGTGCAGGTCTAT CCAGATGGCATTCGGCACATACGAGCAGACAAGAGAGTCAATGAGTGGAAGACCCCTGGA AAGAAAACAATTGTGAAGTGTGCAGTGAACCAGCGACAAGTGGTGATTGCCCTGACAGGA GGAGAGCTGGTCTATTTCGAGATGGATCCTTCAGGACAGCTGAATGAGTACACAGAACGG AAGGAGATGTCAGCAGATGTGGTGTGCATGAGTCTGGCCAATGTACCCCCTGGAGAGCAG CGGTCTCGCTTCCTGGCTGTGGGGCTTGTGGACAACACTGTCAGAATCATCTCCCTGGAT CCCTCAGACTGTTTGCAACCTCTAAGCATGCAGGCTCTCCCAGCCCAGCCTGAGTCCTTG TGTATCGTGGAAATGGGTGGGACTGAGAAGCAGGATGAGCTGGGTGAGAGGGGCTCGATT GGCTTCCTATACCTGAATATTGGGCTACAGAACGGTGTGCTGCTGAGGACTGTCTTGGAC CCTGTCACTGGGGATTTGTCTGATACTCGCACTCGGTACCTGGGGTCCCGTCCTGTGAAG CTCTTCCGAGTCCGAATGCAAGGCCAGGAGGCAGTATTGGCCATGTCAAGCCGCTCATGG TTGAGCTATTCTTACCAATCTCGCTTCCATCTCACCCCACTGTCTTACGAGACACTGGAA TTTGCATCGGGTTTTGCCTCGGAACAGTGTCCCGAGGGCATTGTGGCCATCTCCACCAAC ACCCTACGGATTTTGGCATTAGAGAAGCTCGGTGCTGTCTTCAATCAAGTAGCCTTCCCA CTGCAGTACACACCCAGGAAATTTGTCATCCACCCTGAGAGTAACAACCTTATTATCATT GAAACGGACCACAATGCCTACACTGAGGCCACGAAAGCTCAGAGAAAGCAGCAGATGGCA GAGGAAATGGTGGAAGCAGCAGGGGAGGATGAGCGGGAGCTGGCCGCAGAGATGGCAGCA GCATTCCTCAATGAAAACCTCCCTGAATCCATCTTTGGAGCTCCCAAGGCTGGCAATGGG CAGTGGGCCTCTGTGATCCGAGTGATGAATCCCATTCAAGGGAACACACTGGACCTTGTC CAGCTGGAACAGAATGAGGCAGCTTTTAGTGTGGCTGTGTGCAGGTTTTCCAACACTGGT GAAGACTGGTATGTGCTGGTGGGTGTGGCCAAGGACCTGATACTAAACCCCCGATCTGTG GCAGGGGGCTTCGTCTATACTTACAAGCTTGTGAACAATGGGGAAAAACTGGAGTTTTTG CACAAGACTCCTGTGGAAGAGGTCCCTGCTGCTATTGCCCCATTCCAGGGGAGGGTGTTG ATTGGTGTGGGGAAGCTGTTGCGTGTCTATGACCTGGGAAAGAAGAAGTTACTCCGAAAA TGTGAGAATAAGCATATTGCCAATTATATCTCTGGGATCCAGACTATTGGACATAGGGTA ATTGTATCTGATGTCCAAGAAAGTTTCATCTGGGTTCGCTACAAGCGTAATGAAAACCAG CTTATCATCTTTGCTGATGATACCTACCCCCGATGGGTCACTACAGCCAGCCTCCTGGAC TATGACACTGTGGCTGGGGCAGACAAGTTTGGCAACATATGTGTGGTGAGGCTCCCACCT AACACCAATGATGAAGTAGATGAGGATCCTACAGGAAACAAAGCCCTGTGGGACCGTGGC TTGCTCAATGGGGCCTCCCAGAAGGCAGAGGTGATCATGAACTACCATGTCGGGGAGACG GTGCTGTCCTTGCAGAAGACCACGCTGATCCCTGGAGGCTCAGAATCACTTGTCTATACC ACCTTGTCTGGAGGAATTGGCATCCTTGTGCCATTCACGTCCCATGAGGACCATGACTTC TTCCAGCATGTGGAAATGCACCTGCGGTCTGAACATCCCCCTCTCTGTGGGCGGGACCAC CTCAGCTTTCGCTCCTACTACTTCCCTGTGAAGAATGTGATTGATGGAGACCTCTGTGAG CAGTTCAATTCCATGGAACCCAACAAACAAAAGAACGTCTCTGAAGAACTGGACCGAACC CCACCCGAAGTGTCCAAGAAACTCGAGGATATCCGGACCCGCTACGCCTTCTGA Clone variation with respect to NM_012426.4 717 a=>t;2988 c=>t >OriGene 5' read for NM_012426 unedited
GCGAATTCGGCACGAGGTCTAATGGCGGACGCCAGTATGTTGGAGTTGGTGGTGGCTTAA GTTTTGAAGGGAGGTAGCATCCGTTGGATATCCACACCATCCTTCTCGCTGCAGGCTTTC TTGGACTCCGTACTGTTGGTGTAACCAAGGCCTGGAGGTCTGGGTGGCTCAGGTTTCCTG CAGCCATGTTTCTGTACAACTTAACCTTGCAGAGAGCCACTGGCATCAGCTTTGCCATTC ATGGAAACTTTTCTGGAACCAAACAACAAGAAATTGTTGTTTCCCGTGGGAAGATCTTGG AGCTGCTTCGCCCAGACCCCAACACTGGCAAAGTACATACCCTACTCACTGTGGAAGTAT TCGGTGTTATCCGGTCACTCATGGCCTTTAGGCTGACAGGTGGCACCAAAGACTACATTG TAGTTGGCAGTGACTCTGGTCGAATTGTTATTTTGGAATACCAGCCATCTAAGAATATGT TTGAGAAGATTCACCAAGAAACCTTTGGCAAGAGTGGATGCCGTCGCATCGTTCCTGGCC AGTTCTTAGCTGTGGATCCCAAAGGGCGAGCCGTTATGATTAGTGCCATTGAGAAACAGA AATTGGTGTATATTTTGAACAGAGATGCTGCAGCCCGACTTACCATTTCATCTCCCCTGG AAGCCCACAAAGCANACACTTTAGTGTATCATGTAGTTGGAGTAGATGTCGGATTTGAAA TCCAATGTTTGCTTGTCTGGA |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_012426 |
| Insert Size | 4430 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012426.3, NP_036558.3 |
| RefSeq Size | 4357 bp |
| RefSeq ORF | 3654 bp |
| Locus ID | 23450 |
| UniProt ID | Q15393 |
| Domains | CPSF_A |
| Protein Pathways | Spliceosome |
| Gene Summary | This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq, Jul 2008] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209509 | SF3B3 (Myc-DDK-tagged)-Human splicing factor 3b, subunit 3, 130kDa (SF3B3) |
CNY 8,472.00 |
|
| RC209509L1 | Lenti ORF clone of Human splicing factor 3b, subunit 3, 130kDa (SF3B3), Myc-DDK-tagged |
CNY 10,872.00 |
|
| RC209509L2 | Lenti ORF clone of Human splicing factor 3b, subunit 3, 130kDa (SF3B3), mGFP tagged |
CNY 14,060.00 |
|
| RC209509L3 | Lenti ORF clone of Human splicing factor 3b, subunit 3, 130kDa (SF3B3), Myc-DDK-tagged |
CNY 14,060.00 |
|
| RC209509L4 | Lenti ORF clone of Human splicing factor 3b, subunit 3, 130kDa (SF3B3), mGFP tagged |
CNY 14,060.00 |
|
| RG209509 | SF3B3 (tGFP-tagged) - Human splicing factor 3b, subunit 3, 130kDa (SF3B3) |
CNY 10,072.00 |
|
| SC323872 | SF3B3 (untagged)-Human splicing factor 3b, subunit 3, 130kDa (SF3B3) |
CNY 8,480.00 |
