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HRPT2 (CDC73) (NM_024529) Human Tagged ORF Clone
CAT＃: RC209479
CDC73 (Myc-DDK-tagged)-Human cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (CDC73)
ORF Plasmid: tGFP
"NM_024529" in other vectors (7)
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CNY 3,952.00
CNY 4,560.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C1orf28; FIHP; HPTJT; HRPT1; HRPT2; HYX |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209479 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGTGCTTAGCGTCCTGCGACAGTACAACATCCAGAAGAAGGAGATTGTGGTGAAGGGAGACG AAGTGATCTTCGGGGAGTTCTCCTGGCCCAAGAATGTGAAGACCAACTATGTTGTTTGGGGGACTGGAAA GGAAGGCCAACCCAGAGAGTACTACACATTGGATTCCATTTTATTTCTACTTAATAACGTGCACCTTTCT CATCCTGTTTATGTCCGACGTGCAGCTACTGAAAATATTCCTGTGGTTAGAAGACCTGATCGAAAAGATC TACTTGGATATCTCAATGGTGAAGCGTCAACATCGGCAAGTATAGACAGAAGCGCTCCCTTAGAAATAGG TCTTCAGCGATCTACTCAAGTCAAACGAGCTGCAGATGAAGTTTTAGCAGAAGCAAAGAAACCACGAATT GAGGATGAAGAGTGTGTGCGCCTTGATAAAGAGAGATTGGCTGCCCGTTTGGAGGGTCACAAAGAAGGGA TTGTACAGACTGAACAGATTAGGTCTTTGTCTGAAGCTATGTCAGTGGAAAAAATTGCTGCAATCAAAGC CAAAATTATGGCTAAGAAAAGATCTACTATCAAGACTGATCTAGATGATGACATAACTGCCCTTAAACAG AGGAGTTTTGTGGATGCTGAGGTAGATGTGACCCGAGATATTGTCAGCAGAGAGAGAGTATGGAGGACAC GAACAACTATCTTACAAAGCACAGGAAAGAATTTTTCCAAGAACATTTTTGCAATTCTTCAATCTGTAAA AGCCAGAGAAGAAGGGCGTGCACCTGAACAGCGACCTGCCCCAAATGCAGCACCTGTGGATCCCACTTTG CGCACCAAACAGCCTATCCCAGCTGCCTATAACAGATACGATCAGGAAAGATTCAAAGGAAAAGAAGAAA CGGAAGGCTTCAAAATTGACACTATGGGAACCTACCATGGTATGACACTGAAATCTGTAACGGAGGGTGC ATCTGCCCGGAAGACTCAGACTCCTGCAGCCCAGCCAGTACCAAGACCAGTTTCTCAAGCAAGACCTCCC CCAAATCAGAAGAAAGGATCTCGAACACCCATTATCATAATTCCTGCAGCTACCACCTCTTTAATAACCA TGCTTAATGCAAAAGACCTTCTACAGGACCTGAAATTTGTCCCATCAGATGAAAAGAAGAAACAAGGTTG TCAACGAGAAAATGAAACTCTAATACAAAGAAGAAAAGACCAGATGCAACCAGGGGGCACTGCAATTAGT GTTACAGTACCTTATAGAGTAGTAGACCAGCCCCTTAAACTTATGCCTCAAGACTGGGACCGCGTTGTAG CCGTTTTTGTGCAGGGTCCTGCATGGCAGTTCAAAGGTTGGCCATGGCTTTTGCCTGATGGATCACCAGT TGATATATTTGCTAAAATTAAAGCCTTCCATCTGAAGTATGATGAAGTTCGTCTGGATCCAAATGTTCAG AAATGGGATGTAACAGTATTAGAACTCAGCTATCACAAACGTCATTTGGATAGACCAGTGTTCTTACGGT TTTGGGAAACATTGGACAGGTACATGGTAAAGCATAAATCGCACTTGAGATTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209479 protein sequence
Red=Cloning site Green=Tags(s) MADVLSVLRQYNIQKKEIVVKGDEVIFGEFSWPKNVKTNYVVWGTGKEGQPREYYTLDSILFLLNNVHLS HPVYVRRAATENIPVVRRPDRKDLLGYLNGEASTSASIDRSAPLEIGLQRSTQVKRAADEVLAEAKKPRI EDEECVRLDKERLAARLEGHKEGIVQTEQIRSLSEAMSVEKIAAIKAKIMAKKRSTIKTDLDDDITALKQ RSFVDAEVDVTRDIVSRERVWRTRTTILQSTGKNFSKNIFAILQSVKAREEGRAPEQRPAPNAAPVDPTL RTKQPIPAAYNRYDQERFKGKEETEGFKIDTMGTYHGMTLKSVTEGASARKTQTPAAQPVPRPVSQARPP PNQKKGSRTPIIIIPAATTSLITMLNAKDLLQDLKFVPSDEKKKQGCQRENETLIQRRKDQMQPGGTAIS VTVPYRVVDQPLKLMPQDWDRVVAVFVQGPAWQFKGWPWLLPDGSPVDIFAKIKAFHLKYDEVRLDPNVQ KWDVTVLELSYHKRHLDRPVFLRFWETLDRYMVKHKSHLRF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024529 |
| ORF Size | 1593 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024529.5 |
| RefSeq Size | 5942 bp |
| RefSeq ORF | 1596 bp |
| Locus ID | 79577 |
| UniProt ID | Q6P1J9 |
| Domains | Cdc73 |
| Protein Families | Druggable Genome |
| MW | 60.6 kDa |
| Gene Summary | This gene encodes a tumor suppressor that is involved in transcriptional and post-transcriptional control pathways. The protein is a component of the the PAF protein complex, which associates with the RNA polymerase II subunit POLR2A and with a histone methyltransferase complex. This protein appears to facilitate the association of 3' mRNA processing factors with actively-transcribed chromatin. Mutations in this gene have been linked to hyperparathyroidism-jaw tumor syndrome, familial isolated hyperparathyroidism, and parathyroid carcinoma. [provided by RefSeq, Jul 2009] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Large intragenic deletion of CDC73 (exons 4-10) in a three-generation hyperparathyroidism-jaw tumor (HPT-JT) syndrome family
,Guarnieri, V;Seaberg, RM;Kelly, C;Jean Davidson, M;Raphael, S;Shuen, AY;Baorda, F;Palumbo, O;Scillitani, A;Hendy, GN;Cole, DEC;,
BMC Med. Genet.
,PubMed ID 28774260
[HRPT2]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209479L1 | Lenti ORF clone of Human cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (CDC73), Myc-DDK-tagged |
CNY 6,352.00 |
|
| RC209479L2 | Lenti ORF clone of Human cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (CDC73), mGFP tagged |
CNY 6,460.00 |
|
| RC209479L3 | Lenti ORF clone of Human cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (CDC73), Myc-DDK-tagged |
CNY 6,352.00 |
|
| RC209479L4 | Lenti ORF clone of Human cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (CDC73), mGFP tagged |
CNY 6,352.00 |
|
| RG209479 | CDC73 (tGFP-tagged) - Human cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (CDC73) |
CNY 5,552.00 |
|
| SC112162 | CDC73 (untagged)-Human cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (CDC73) |
CNY 3,960.00 |
|
| SC324236 | CDC73 (untagged)-Human cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (CDC73) |
CNY 3,960.00 |
