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MRE11 (NM_005591) Human Tagged ORF Clone
CAT＃: RC209414
MRE11A (Myc-DDK-tagged)-Human MRE11 meiotic recombination 11 homolog A (S. cerevisiae) (MRE11A), transcript variant 1
ORF Plasmid: tGFP
"NM_005591" in other vectors (7)
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CNY 5,184.00
CNY 5,800.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ATLD; HNGS1; MRE11A; MRE11B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209414 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTACTGCAGATGCACTTGATGATGAAAACACATTTAAAATATTAGTTGCAACAGATATTCATCTTG GATTTATGGAGAAAGATGCAGTCAGAGGAAATGATACGTTTGTAACACTCGATGAAATTTTAAGACTTGC CCAGGAAAATGAAGTGGATTTTATTTTGTTAGGTGGTGATCTTTTTCATGAAAATAAGCCCTCAAGGAAA ACATTACATACCTGCCTCGAGTTATTAAGAAAATATTGTATGGGTGATCGGCCTGTCCAGTTTGAAATTC TCAGTGATCAGTCAGTCAACTTTGGTTTTAGTAAGTTTCCATGGGTGAACTATCAAGATGGCAACCTCAA CATTTCAATTCCAGTGTTTAGTATTCATGGCAATCATGACGATCCCACAGGGGCAGATGCACTTTGTGCC TTGGACATTTTAAGTTGTGCTGGATTTGTAAATCACTTTGGACGTTCAATGTCTGTGGAGAAGATAGACA TTAGTCCGGTTTTGCTTCAAAAAGGAAGCACAAAGATTGCGCTATATGGTTTAGGATCCATTCCAGATGA AAGGCTCTATCGAATGTTTGTCAATAAAAAAGTAACAATGTTGAGACCAAAGGAAGATGAGAACTCTTGG TTTAACTTATTTGTGATTCATCAGAACAGGAGTAAACATGGAAGTACTAACTTCATTCCAGAACAATTTT TGGATGACTTCATTGATCTTGTTATCTGGGGCCATGAACATGAGTGTAAAATAGCTCCAACCAAAAATGA ACAACAGCTGTTTTATATCTCACAACCTGGAAGCTCAGTGGTTACTTCTCTTTCCCCAGGAGAAGCTGTA AAGAAACATGTTGGTTTGCTGCGTATTAAAGGGAGGAAGATGAATATGCATAAAATTCCTCTTCACACAG TGCGGCAGTTTTTCATGGAGGATATTGTTCTAGCTAATCATCCAGACATTTTTAACCCAGATAATCCTAA AGTAACCCAAGCCATACAAAGCTTCTGTTTGGAGAAGATTGAAGAAATGCTTGAAAATGCTGAACGGGAA CGTCTGGGTAATTCTCACCAGCCAGAGAAGCCTCTTGTACGACTGCGAGTGGACTATAGTGGAGGTTTTG AACCTTTCAGTGTTCTTCGCTTTAGCCAGAAATTTGTGGATCGGGTAGCTAATCCAAAAGACATTATCCA TTTTTTCAGGCATAGAGAACAAAAGGAAAAAACAGGAGAAGAGATCAACTTTGGGAAACTTATCACAAAG CCTTCAGAAGGAACAACTTTAAGGGTAGAAGATCTTGTAAAACAGTACTTTCAAACCGCAGAGAAGAATG TGCAGCTCTCACTGCTAACAGAAAGAGGGATGGGTGAAGCAGTACAAGAATTTGTGGACAAGGAGGAGAA AGATGCCATTGAGGAATTAGTGAAATACCAGTTGGAAAAAACACAGCGATTTCTTAAAGAACGTCATATT GATGCCCTCGAAGACAAAATCGATGAGGAGGTACGTCGTTTCAGAGAAACCAGACAAAAAAATACTAATG AAGAAGATGATGAAGTCCGTGAGGCTATGACCAGGGCCAGAGCACTCAGATCTCAGTCAGAGGAGTCTGC TTCTGCCTTTAGTGCTGATGACCTTATGAGTATAGATTTAGCAGAACAGATGGCTAATGACTCTGATGAT AGCATCTCAGCAGCAACCAACAAAGGAAGAGGCCGAGGAAGAGGTCGAAGAGGTGGAAGAGGGCAGAATT CAGCATCGAGAGGAGGGTCTCAAAGAGGAAGAGCAGACACTGGTCTGGAGACTTCTACCCGTAGCAGGAA CTCAAAGACTGCTGTGTCAGCATCTAGAAATATGTCTATTATAGATGCCTTTAAATCTACAAGACAGCAG CCTTCCCGAAATGTCACTACTAAGAATTATTCAGAGGTGATTGAGGTAGATGAATCAGATGTGGAAGAAG ACATTTTTCCTACCACTTCAAAGACAGATCAAAGGTGGTCCAGCACATCATCCAGCAAAATCATGTCCCA GAGTCAAGTATCGAAAGGGGTTGATTTTGAATCAAGTGAGGATGATGATGATGATCCTTTTATGAACACT AGTTCTTTAAGAAGAAATAGAAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209414 protein sequence
Red=Cloning site Green=Tags(s) MSTADALDDENTFKILVATDIHLGFMEKDAVRGNDTFVTLDEILRLAQENEVDFILLGGDLFHENKPSRK TLHTCLELLRKYCMGDRPVQFEILSDQSVNFGFSKFPWVNYQDGNLNISIPVFSIHGNHDDPTGADALCA LDILSCAGFVNHFGRSMSVEKIDISPVLLQKGSTKIALYGLGSIPDERLYRMFVNKKVTMLRPKEDENSW FNLFVIHQNRSKHGSTNFIPEQFLDDFIDLVIWGHEHECKIAPTKNEQQLFYISQPGSSVVTSLSPGEAV KKHVGLLRIKGRKMNMHKIPLHTVRQFFMEDIVLANHPDIFNPDNPKVTQAIQSFCLEKIEEMLENAERE RLGNSHQPEKPLVRLRVDYSGGFEPFSVLRFSQKFVDRVANPKDIIHFFRHREQKEKTGEEINFGKLITK PSEGTTLRVEDLVKQYFQTAEKNVQLSLLTERGMGEAVQEFVDKEEKDAIEELVKYQLEKTQRFLKERHI DALEDKIDEEVRRFRETRQKNTNEEDDEVREAMTRARALRSQSEESASAFSADDLMSIDLAEQMANDSDD SISAATNKGRGRGRGRRGGRGQNSASRGGSQRGRADTGLETSTRSRNSKTAVSASRNMSIIDAFKSTRQQ PSRNVTTKNYSEVIEVDESDVEEDIFPTTSKTDQRWSSTSSSKIMSQSQVSKGVDFESSEDDDDDPFMNT SSLRRNRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005591 |
| ORF Size | 2124 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005591.4 |
| RefSeq Size | 5141 bp |
| RefSeq ORF | 2127 bp |
| Locus ID | 4361 |
| UniProt ID | P49959 |
| Domains | Metallophos, Mre11_DNA_bind |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Homologous recombination, Non-homologous end-joining |
| MW | 80.6 kDa |
| Gene Summary | This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Efficient Recreation of t (11; 22) EWSR1-FLI1+ in Human Stem Cells Using CRISPR/Cas9
,Torres-Ruiz, R;Martinez-Lage, M;Martin, MC;Garcia, A;Bueno, C;o, JC;Ramirez, JC;Menendez, P;Cigudosa, JC;Rodriguez-Perales, S;,
Stem Cell Reports
,PubMed ID 28494941
[MRE11]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209414L1 | Lenti ORF clone of Human MRE11 meiotic recombination 11 homolog A (S. cerevisiae) (MRE11A), transcript variant 1, Myc-DDK-tagged |
CNY 7,584.00 |
|
| RC209414L2 | Lenti ORF clone of Human MRE11 meiotic recombination 11 homolog A (S. cerevisiae) (MRE11A), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RC209414L3 | Lenti ORF clone of Human MRE11 meiotic recombination 11 homolog A (S. cerevisiae) (MRE11A), transcript variant 1, Myc-DDK-tagged |
CNY 7,584.00 |
|
| RC209414L4 | Lenti ORF clone of Human MRE11 meiotic recombination 11 homolog A (S. cerevisiae) (MRE11A), transcript variant 1, mGFP tagged |
CNY 7,584.00 |
|
| RG209414 | MRE11A (tGFP-tagged) - Human MRE11 meiotic recombination 11 homolog A (S. cerevisiae) (MRE11A), transcript variant 1 |
CNY 6,784.00 |
|
| SC116673 | MRE11A (untagged)-Human MRE11 meiotic recombination 11 homolog A (S. cerevisiae) (MRE11A), transcript variant 1 |
CNY 5,192.00 |
|
| SC322679 | MRE11A (untagged)-Human MRE11 meiotic recombination 11 homolog A (S. cerevisiae) (MRE11A), transcript variant 1 |
CNY 5,192.00 |
