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NPEPPS (NM_006310) Human Tagged ORF Clone
CAT＃: RC209037
NPEPPS (Myc-DDK-tagged)-Human aminopeptidase puromycin sensitive (NPEPPS)
ORF Plasmid: tGFP
"NM_006310" in other vectors (7)
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CNY 6,728.00
CNY 9,690.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AAP-S; MP100; PSA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209037 representing NM_006310
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCTGGCAGCTGCCGCCCCCTCCCTCGCTCGCCGCCTGCTCTTCCTCGGCCCTCCGCCTCCTCCCC TCCTCCTTCTCGTCTTCAGCCGCTCCTCTCGCCGCCGCCTCCACAGCCTGGGCCTCGCCGCGATGCCGGA GAAGAGGCCCTTCGAGCGGCTGCCTGCCGATGTCTCCCCCATCAACTACAGCCTTTGCCTCAAGCCCGAC TTGCTGGACTTCACCTTCGAGGGCAAGCTGGAGGCCGCCGCCCAGGTGAGGCAGGCGACTAATCAGATTG TGATGAATTGTGCTGATATTGATATTATTACAGCTTCATATGCACCAGAAGGAGATGAAGAAATACATGC TACAGGATTTAACTATCAGAATGAAGATGAAAAAGTCACCTTGTCTTTCCCTAGTACTCTGCAAACAGGT ACGGGAACCTTAAAGATAGATTTTGTTGGAGAGCTGAATGACAAAATGAAAGGTTTCTATAGAAGTAAAT ATACTACCCCTTCTGGAGAGGTGCGCTATGCTGCTGTAACACAGTTTGAGGCTACTGATGCCCGAAGGGC TTTTCCTTGCTGGGATGAGCCTGCTATCAAAGCAACTTTTGATATCTCATTGGTTGTTCCTAAAGACAGA GTAGCTTTATCAAACATGAATGTAATTGACCGGAAACCATACCCTGATGATGAAAATTTAGTGGAAGTGA AGTTTGCCCGCACACCTGTTATGTCTACATATCTGGTGGCATTTGTTGTGGGTGAATATGACTTTGTAGA AACAAGGTCAAAAGATGGTGTGTGTGTCCGTGTTTACACTCCTGTTGGCAAAGCAGAGCAAGGAAAATTT GCGTTAGAGGTTGCTGCTAAAACCTTGCCTTTTTATAAGGACTACTTCAATGTTCCTTATCCTCTACCTA AAATTGATCTCATTGCTATTGCAGACTTTGCAGCTGGTGCCATGGAGAACTGGGGCCTTGTTACTTATAG GGAGACTGCATTGCTTATTGATCCAAAAAATTCCTGTTCTTCATCCCGCCAGTGGGTTGCTCTGGTTGTG GGACATGAACTCGCCCATCAATGGTTTGGAAATCTTGTTACTATGGAATGGTGGACTCATCTTTGGTTAA ATGAAGGTTTTGCATCCTGGATTGAATATCTGTGTGTAGACCACTGCTTCCCAGAGTATGATATTTGGAC TCAGTTTGTTTCTGCTGATTACACCCGTGCCCAGGAGCTTGACGCCTTAGATAACAGCCATCCTATTGAA GTCAGTGTGGGCCATCCATCTGAGGTTGATGAGATATTTGATGCTATATCATATAGCAAAGGTGCATCTG TCATCCGAATGCTGCATGACTACATTGGGGATAAGGACTTTAAGAAAGGAATGAACATGTATTTAACCAA GTTCCAACAAAAGAATGCTGCCACAGAGGATCTCTGGGAAAGTTTAGAAAATGCTAGTGGTAAACCTATA GCAGCTGTGATGAATACCTGGACCAAACAAATGGGATTTCCCCTCATTTATGTGGAAGCTGAACAGGTAG AAGATGACAGATTATTGAGGTTGTCCCAAAAGAAGTTCTGTGCTGGTGGGTCATATGTTGGTGAAGATTG TCCCCAGTGGATGGTCCCTATCACAATCTCTACTAGTGAAGACCCCAACCAGGCCAAACTAAAAATTCTA ATGGACAAGCCAGAGATGAATGTGGTTTTGAAAAATGTCAAACCAGACCAATGGGTGAAGTTAAACTTAG GAACAGTTGGGTTTTATCGGACCCAGTACAGCTCTGCCATGCTGGAAAGTTTATTACCAGGCATTCGTGA CCTTTCTCTGCCCCCTGTGGATCGACTTGGATTACAGAATGACCTCTTCTCCTTGGCTCGAGCTGGAATC ATTAGCACTGTAGAGGTTCTAAAAGTCATGGAGGCTTTTGTGAATGAGCCCAATTATACTGTATGGAGCG ACCTGAGCTGTAACCTGGGGATTCTCTCAACTCTCTTGTCCCACACAGACTTCTATGAGGAAATCCAGGA GTTTGTGAAAGATGTCTTTTCACCTATAGGGGAGAGACTGGGCTGGGACCCCAAACCTGGAGAAGGTCAT CTCGATGCACTCCTGAGGGGCTTGGTTCTGGGAAAACTAGGAAAAGCAGGACATAAGGCAACGTTAGAAG AAGCCCGTCGTCGGTTTAAGGACCACGTGGAAGGAAAACAGATTCTCTCCGCTGATCTGAGGAGTCCTGT CTATCTGACTGTTTTGAAGCATGGTGATGGCACTACTTTAGATATTATGTTAAAACTTCATAAACAAGCA GATATGCAAGAAGAGAAAAACCGAATCGAAAGAGTCCTTGGCGCTACTCTTTTGCCTGACCTGATTCAAA AAGTCCTCACGTTTGCACTTTCAGAAGAGGTACGTCCACAGGACACTGTATCGGTAATTGGTGGAGTAGC TGGAGGCAGCAAGCATGGTAGGAAAGCTGCTTGGAAATTCATAAAGGACAACTGGGAAGAACTTTATAAC CGATACCAGGGAGGATTCTTAATATCCAGACTAATAAAGCTATCAGTTGAGGGATTTGCAGTTGATAAAA TGGCTGGAGAGGTTAAGGCTTTCTTCGAGAGTCACCCAGCTCCTTCAGCTGAGCGTACCATCCAGCAGTG TTGTGAAAATATTCTGCTGAATGCTGCCTGGCTAAAGCGAGATGCTGAGAGCATCCACCAGTACCTCCTT CAGCGGAAGGCCTCACCACCCACAGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209037 representing NM_006310
Red=Cloning site Green=Tags(s) MWLAAAAPSLARRLLFLGPPPPPLLLLVFSRSSRRRLHSLGLAAMPEKRPFERLPADVSPINYSLCLKPD LLDFTFEGKLEAAAQVRQATNQIVMNCADIDIITASYAPEGDEEIHATGFNYQNEDEKVTLSFPSTLQTG TGTLKIDFVGELNDKMKGFYRSKYTTPSGEVRYAAVTQFEATDARRAFPCWDEPAIKATFDISLVVPKDR VALSNMNVIDRKPYPDDENLVEVKFARTPVMSTYLVAFVVGEYDFVETRSKDGVCVRVYTPVGKAEQGKF ALEVAAKTLPFYKDYFNVPYPLPKIDLIAIADFAAGAMENWGLVTYRETALLIDPKNSCSSSRQWVALVV GHELAHQWFGNLVTMEWWTHLWLNEGFASWIEYLCVDHCFPEYDIWTQFVSADYTRAQELDALDNSHPIE VSVGHPSEVDEIFDAISYSKGASVIRMLHDYIGDKDFKKGMNMYLTKFQQKNAATEDLWESLENASGKPI AAVMNTWTKQMGFPLIYVEAEQVEDDRLLRLSQKKFCAGGSYVGEDCPQWMVPITISTSEDPNQAKLKIL MDKPEMNVVLKNVKPDQWVKLNLGTVGFYRTQYSSAMLESLLPGIRDLSLPPVDRLGLQNDLFSLARAGI ISTVEVLKVMEAFVNEPNYTVWSDLSCNLGILSTLLSHTDFYEEIQEFVKDVFSPIGERLGWDPKPGEGH LDALLRGLVLGKLGKAGHKATLEEARRRFKDHVEGKQILSADLRSPVYLTVLKHGDGTTLDIMLKLHKQA DMQEEKNRIERVLGATLLPDLIQKVLTFALSEEVRPQDTVSVIGGVAGGSKHGRKAAWKFIKDNWEELYN RYQGGFLISRLIKLSVEGFAVDKMAGEVKAFFESHPAPSAERTIQQCCENILLNAAWLKRDAESIHQYLL QRKASPPTV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006310 |
| ORF Size | 2757 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006310.4 |
| RefSeq Size | 4364 bp |
| RefSeq ORF | 2760 bp |
| Locus ID | 9520 |
| UniProt ID | P55786 |
| Domains | Peptidase_M1 |
| Protein Families | Druggable Genome, Protease |
| MW | 103.1 kDa |
| Gene Summary | This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209037L1 | Lenti ORF clone of Human aminopeptidase puromycin sensitive (NPEPPS), Myc-DDK-tagged |
CNY 9,128.00 |
|
| RC209037L2 | Lenti ORF clone of Human aminopeptidase puromycin sensitive (NPEPPS), mGFP tagged |
CNY 9,128.00 |
|
| RC209037L3 | Lenti ORF clone of Human aminopeptidase puromycin sensitive (NPEPPS), Myc-DDK-tagged |
CNY 9,128.00 |
|
| RC209037L4 | Lenti ORF clone of Human aminopeptidase puromycin sensitive (NPEPPS), mGFP tagged |
CNY 11,590.00 |
|
| RG209037 | NPEPPS (tGFP-tagged) - Human aminopeptidase puromycin sensitive (NPEPPS) |
CNY 10,640.00 |
|
| SC128264 | NPEPPS (untagged)-Human aminopeptidase puromycin sensitive (NPEPPS) |
CNY 6,416.00 |
|
| SC318391 | NPEPPS (untagged)-Human aminopeptidase puromycin sensitive (NPEPPS) |
CNY 17,670.00 |
