NPEPPS (NM_006310) Human Untagged Clone

CAT#: SC318391

NPEPPS (untagged)-Human aminopeptidase puromycin sensitive (NPEPPS)



  "NM_006310" in other vectors (7)

CNY 17,670.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal antibody to NPEPPS (aminopeptidase puromycin sensitive)
    • 100 ul

CNY 6,281.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms AAP-S; MP100; PSA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC318391 representing NM_006310.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTGGCTGGCAGCTGCCGCCCCCTCCCTCGCTCGCCGCCTGCTCTTCCTCGGCCCTCCGCCTCCTCCC
CTCCTCCTTCTCGTCTTCAGCCGCTCCTCTCGCCGCCGCCTCCACAGCCTGGGCCTCGCCGCGATGCCG
GAGAAGAGGCCCTTCGAGCGGCTGCCTGCCGATGTCTCCCCCATCAACTACAGCCTTTGCCTCAAGCCC
GACTTGCTGGACTTCACCTTCGAGGGCAAGCTGGAGGCCGCCGCCCAGGTGAGGCAGGCGACTAATCAG
ATTGTGATGAATTGTGCTGATATTGATATTATTACAGCTTCATATGCACCAGAAGGAGATGAAGAAATA
CATGCTACAGGATTTAACTATCAGAATGAAGATGAAAAAGTCACCTTGTCTTTCCCTAGTACTCTGCAA
ACAGGTACGGGAACCTTAAAGATAGATTTTGTTGGAGAGCTGAATGACAAAATGAAAGGTTTCTATAGA
AGTAAATATACTACCCCTTCTGGAGAGGTGCGCTATGCTGCTGTAACACAGTTTGAGGCTACTGATGCC
CGAAGGGCTTTTCCTTGCTGGGATGAGCCTGCTATCAAAGCAACTTTTGATATCTCATTGGTTGTTCCT
AAAGACAGAGTAGCTTTATCAAACATGAATGTAATTGACCGGAAACCATACCCTGATGATGAAAATTTA
GTGGAAGTGAAGTTTGCCCGCACACCTGTTATGTCTACATATCTGGTGGCATTTGTTGTGGGTGAATAT
GACTTTGTAGAAACAAGGTCAAAAGATGGTGTGTGTGTCCGTGTTTACACTCCTGTTGGCAAAGCAGAG
CAAGGAAAATTTGCGTTAGAGGTTGCTGCTAAAACCTTGCCTTTTTATAAGGACTACTTCAATGTTCCT
TATCCTCTACCTAAAATTGATCTCATTGCTATTGCAGACTTTGCAGCTGGTGCCATGGAGAACTGGGGC
CTTGTTACTTATAGGGAGACTGCATTGCTTATTGATCCAAAAAATTCCTGTTCTTCATCCCGCCAGTGG
GTTGCTCTGGTTGTGGGACATGAACTCGCCCATCAATGGTTTGGAAATCTTGTTACTATGGAATGGTGG
ACTCATCTTTGGTTAAATGAAGGTTTTGCATCCTGGATTGAATATCTGTGTGTAGACCACTGCTTCCCA
GAGTATGATATTTGGACTCAGTTTGTTTCTGCTGATTACACCCGTGCCCAGGAGCTTGACGCCTTAGAT
AACAGCCATCCTATTGAAGTCAGTGTGGGCCATCCATCTGAGGTTGATGAGATATTTGATGCTATATCA
TATAGCAAAGGTGCATCTGTCATCCGAATGCTGCATGACTACATTGGGGATAAGGACTTTAAGAAAGGA
ATGAACATGTATTTAACCAAGTTCCAACAAAAGAATGCTGCCACAGAGGATCTCTGGGAAAGTTTAGAA
AATGCTAGTGGTAAACCTATAGCAGCTGTGATGAATACCTGGACCAAACAAATGGGATTTCCCCTCATT
TATGTGGAAGCTGAACAGGTAGAAGATGACAGATTATTGAGGTTGTCCCAAAAGAAGTTCTGTGCTGGT
GGGTCATATGTTGGTGAAGATTGTCCCCAGTGGATGGTCCCTATCACAATCTCTACTAGTGAAGACCCC
AACCAGGCCAAACTAAAAATTCTAATGGACAAGCCAGAGATGAATGTGGTTTTGAAAAATGTCAAACCA
GACCAATGGGTGAAGTTAAACTTAGGAACAGTTGGGTTTTATCGGACCCAGTACAGCTCTGCCATGCTG
GAAAGTTTATTACCAGGCATTCGTGACCTTTCTCTGCCCCCTGTGGATCGACTTGGATTACAGAATGAC
CTCTTCTCCTTGGCTCGAGCTGGAATCATTAGCACTGTAGAGGTTCTAAAAGTCATGGAGGCTTTTGTG
AATGAGCCCAATTATACTGTATGGAGCGACCTGAGCTGTAACCTGGGGATTCTCTCAACTCTCTTGTCC
CACACAGACTTCTATGAGGAAATCCAGGAGTTTGTGAAAGATGTCTTTTCACCTATAGGGGAGAGACTG
GGCTGGGACCCCAAACCTGGAGAAGGTCATCTCGATGCACTCCTGAGGGGCTTGGTTCTGGGAAAACTA
GGAAAAGCAGGACATAAGGCAACGTTAGAAGAAGCCCGTCGTCGGTTTAAGGACCACGTGGAAGGAAAA
CAGATTCTCTCCGCTGATCTGAGGAGTCCTGTCTATCTGACTGTTTTGAAGCATGGTGATGGCACTACT
TTAGATATTATGTTAAAACTTCATAAACAAGCAGATATGCAAGAAGAGAAAAACCGAATCGAAAGAGTC
CTTGGCGCTACTCTTTTGCCTGACCTGATTCAAAAAGTCCTCACGTTTGCACTTTCAGAAGAGGTACGT
CCACAGGACACTGTATCGGTAATTGGTGGAGTAGCTGGAGGCAGCAAGCATGGTAGGAAAGCTGCTTGG
AAATTCATAAAGGACAACTGGGAAGAACTTTATAACCGATACCAGGGAGGATTCTTAATATCCAGACTA
ATAAAGCTATCAGTTGAGGGATTTGCAGTTGATAAAATGGCTGGAGAGGTTAAGGCTTTCTTCGAGAGT
CACCCAGCTCCTTCAGCTGAGCGTACCATCCAGCAGTGTTGTGAAAATATTCTGCTGAATGCTGCCTGG
CTAAAGCGAGATGCTGAGAGCATCCACCAGTACCTCCTTCAGCGGAAGGCCTCACCACCCACAGTGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_006310
Insert Size 2760 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006310.3
RefSeq Size 4364 bp
RefSeq ORF 2760 bp
Locus ID 9520
UniProt ID P55786
Domains Peptidase_M1
Protein Families Druggable Genome, Protease
MW 103.3 kDa
Gene Summary This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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