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DIS3 (NM_014953) Human Tagged ORF Clone
CAT＃: RC208907
DIS3 (Myc-DDK-tagged)-Human DIS3 mitotic control homolog (S. cerevisiae) (DIS3), transcript variant 1
ORF Plasmid: tGFP
"NM_014953" in other vectors (6)
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CNY 10,520.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2810028N01Rik; dis3p; EXOSC11; KIAA1008; RRP44 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208907 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAAGTCCAAGACGTTCTTAAAAAAGACCCGGGCGGGCGGCGTGATGAAGATCGTGCGCGAGCACT ACCTGCGAGACGACATCGGCTGCGGTGCGCCCGGGTGCGCAGCGTGTGGAGGGGCGCACGAGGGGCCGGC CCTGGAGCCGCAGCCCCAGGACCCGGCGAGCAGCGTCTGCCCGCAACCGCACTACTTGCTGCCCGACACT AATGTGTTACTGCACCAGATTGATGTTCTTGAGGACCCTGCCATCAGGAATGTAATTGTGCTACAAACAG TTCTTCAAGAAGTGAGAAATCGCAGTGCCCCCGTATATAAACGCATCCGAGATGTGACTAATAACCAAGA GAAGCATTTCTATACTTTCACTAATGAGCACCATAGAGAAACCTATGTAGAACAAGAACAGGGAGAAAAT GCTAATGACAGGAATGATAGAGCGATTCGAGTAGCAGCAAAATGGTACAATGAACATTTGAAAAAAATGT CAGCAGACAACCAGCTGCAAGTTATCTTCATAACAAATGACAGGAGAAACAAAGAGAAAGCCATAGAAGA AGGAATACCAGCTTTCACTTGTGAAGAATATGTAAAGAGCCTAACTGCTAACCCCGAACTCATAGATCGT CTTGCTTGTTTGTCTGAAGAAGGGAATGAAATAGAAAGTGGAAAAATAATATTTTCAGAGCATCTTCCCT TAAGTAAGCTACAGCAAGGCATAAAATCTGGTACATACCTTCAAGGAACATTTAGAGCTAGCAGGGAAAA TTACTTGGAAGCTACAGTATGGATTCATGGCGACAATGAAGAAAATAAAGAGATAATCTTACAGGGACTT AAACATTTAAACAGAGCTGTTCACGAAGATATTGTGGCTGTGGAGCTTCTCCCCAAGAGTCAGTGGGTAG CACCATCTTCTGTGGTTTTACATGATGAAGGTCAAAATGAAGAAGATGTGGAGAAAGAAGAAGAGAGAGA ACGAATGCTTAAGACTGCTGTAAGCGAGAAAATGTTGAAGCCTACAGGTAGAGTTGTAGGAATAATAAAA AGGAATTGGAGACCATATTGTGGCATGCTTTCCAAGTCTGACATTAAGGAGTCAAGAAGACATCTCTTTA CACCTGCTGATAAGAGAATCCCTCGAATTCGCATAGAAACCAGACAGGCTTCCACATTAGAAGGACGGAG AATTATTGTTGCTATTGATGGTTGGCCCAGAAATTCCAGATATCCAAATGGACACTTTGTGAGAAATTTA GGTGATGTTGGAGAGAAAGAGACTGAAACAGAAGTTTTGTTACTTGAACACGATGTTCCCCATCAGCCTT TTTCACAGGCTGTTCTTAGTTTTCTGCCAAAGATGCCCTGGAGCATTACTGAAAAGGACATGAAAAACCG AGAAGACCTGAGGCATCTGTGTATTTGTAGTGTAGACCCACCAGGATGTACTGATATAGACGATGCTCTA CATTGTCGAGAACTCGAAAATGGAAATTTGGAGGTTGGTGTTCATATTGCTGATGTGAGCCATTTTATTA GGCCAGGAAATGCCTTGGATCAAGAATCAGCCAGAAGAGGAACAACTGTGTATCTTTGTGAAAAGAGGAT TGACATGGTTCCAGAGTTGCTTAGCTCTAACTTGTGTTCCTTAAAATGTGACGTGGACAGGCTGGCATTT TCATGTATTTGGGAAATGAATCACAATGCTGAAATCTTAAAAACGAAGTTTACCAAAAGTGTTATTAATT CAAAGGCATCTCTGACATATGCTGAAGCTCAGTTGAGAATTGATTCAGCAAACATGAATGATGATATTAC CACTAGTCTCCGTGGACTGAATAAACTAGCCAAAATTCTGAAGAAAAGAAGGATTGAAAAAGGGGCTTTG ACTCTATCCTCTCCTGAAGTTCGATTCCACATGGACAGTGAAACTCACGATCCTATAGATCTGCAGACCA AGGAACTTAGGGAAACAAATTCCATGGTTGAAGAATTTATGTTACTTGCCAATATTTCTGTTGCAAAAAA AATTCATGAGGAATTTTCTGAACATGCTCTGCTTCGAAAACATCCTGCTCCACCTCCATCAAATTATGAA ATTCTTGTTAAGGCAGCCAGGTCAAGGAATTTGGAAATTAAGACTGATACAGCCAAGTCTTTGGCTGAGT CTTTGGATCAGGCCGAATCTCCTACTTTTCCATATCTAAACACTCTGTTGAGAATATTAGCCACTCGCTG TATGATGCAAGCTGTGTACTTCTGTTCTGGAATGGATAATGATTTTCATCACTATGGCTTAGCGTCTCCA ATATACACACATTTTACTTCACCCATTAGAAGATACGCAGATGTCATTGTTCATCGGCTTTTGGCTGTGG CTATTGGGGCTGACTGTACTTATCCAGAGTTGACAGACAAACACAAGCTTGCAGATATATGTAAAAATCT AAATTTCCGGCACAAAATGGCTCAATATGCCCAACGTGCATCAGTGGCTTTTCATACCCAGTTATTCTTC AAAAGCAAAGGAATAGTAAGTGAAGAAGCCTATATTTTATTTGTAAGAAAGAATGCCATTGTGGTATTAA TTCCAAAGTATGGTTTAGAAGGGACAGTCTTTTTTGAAGAAAAGGACAAACCAAACCCACAGCTTATTTA TGATGATGAGATACCCTCACTTAAAATAGAAGATACAGTGTTCCATGTATTTGATAAAGTTAAAGTGAAA ATCATGTTAGACTCATCTAATCTTCAACATCAGAAGATCCGAATGTCCCTGGTAGAACCACAGATACCAG GAATAAGCATTCCTACTGATACTTCAAACATGGACCTTAATGGACCAAAGAAAAAGAAGATGAAGCTTGG AAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208907 protein sequence
Red=Cloning site Green=Tags(s) MLKSKTFLKKTRAGGVMKIVREHYLRDDIGCGAPGCAACGGAHEGPALEPQPQDPASSVCPQPHYLLPDT NVLLHQIDVLEDPAIRNVIVLQTVLQEVRNRSAPVYKRIRDVTNNQEKHFYTFTNEHHRETYVEQEQGEN ANDRNDRAIRVAAKWYNEHLKKMSADNQLQVIFITNDRRNKEKAIEEGIPAFTCEEYVKSLTANPELIDR LACLSEEGNEIESGKIIFSEHLPLSKLQQGIKSGTYLQGTFRASRENYLEATVWIHGDNEENKEIILQGL KHLNRAVHEDIVAVELLPKSQWVAPSSVVLHDEGQNEEDVEKEEERERMLKTAVSEKMLKPTGRVVGIIK RNWRPYCGMLSKSDIKESRRHLFTPADKRIPRIRIETRQASTLEGRRIIVAIDGWPRNSRYPNGHFVRNL GDVGEKETETEVLLLEHDVPHQPFSQAVLSFLPKMPWSITEKDMKNREDLRHLCICSVDPPGCTDIDDAL HCRELENGNLEVGVHIADVSHFIRPGNALDQESARRGTTVYLCEKRIDMVPELLSSNLCSLKCDVDRLAF SCIWEMNHNAEILKTKFTKSVINSKASLTYAEAQLRIDSANMNDDITTSLRGLNKLAKILKKRRIEKGAL TLSSPEVRFHMDSETHDPIDLQTKELRETNSMVEEFMLLANISVAKKIHEEFSEHALLRKHPAPPPSNYE ILVKAARSRNLEIKTDTAKSLAESLDQAESPTFPYLNTLLRILATRCMMQAVYFCSGMDNDFHHYGLASP IYTHFTSPIRRYADVIVHRLLAVAIGADCTYPELTDKHKLADICKNLNFRHKMAQYAQRASVAFHTQLFF KSKGIVSEEAYILFVRKNAIVVLIPKYGLEGTVFFEEKDKPNPQLIYDDEIPSLKIEDTVFHVFDKVKVK IMLDSSNLQHQKIRMSLVEPQIPGISIPTDTSNMDLNGPKKKKMKLGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014953 |
| ORF Size | 2874 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014953.5 |
| RefSeq Size | 7589 bp |
| RefSeq ORF | 2877 bp |
| Locus ID | 22894 |
| UniProt ID | Q9Y2L1 |
| Domains | RNB, PINc |
| Protein Pathways | RNA degradation |
| MW | 109.1 kDa |
| Gene Summary | Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208907L1 | Lenti ORF clone of Human DIS3 mitotic control homolog (S. cerevisiae) (DIS3), transcript variant 1, Myc-DDK-tagged |
CNY 12,920.00 |
|
| RC208907L2 | Lenti ORF clone of Human DIS3 mitotic control homolog (S. cerevisiae) (DIS3), transcript variant 1, mGFP tagged |
CNY 9,030.00 |
|
| RC208907L3 | Lenti ORF clone of Human DIS3 mitotic control homolog (S. cerevisiae) (DIS3), transcript variant 1, Myc-DDK-tagged |
CNY 12,920.00 |
|
| RC208907L4 | Lenti ORF clone of Human DIS3 mitotic control homolog (S. cerevisiae) (DIS3), transcript variant 1, mGFP tagged |
CNY 12,920.00 |
|
| RG208907 | DIS3 (tGFP-tagged) - Human DIS3 mitotic control homolog (S. cerevisiae) (DIS3), transcript variant 1 |
CNY 12,120.00 |
|
| SC114689 | DIS3 (untagged)-Human DIS3 mitotic control homolog (S. cerevisiae) (DIS3), transcript variant 1 |
CNY 10,536.00 |
