HDAC8 (NM_018486) Human Tagged ORF Clone
CAT#: RC208390
HDAC8 (Myc-DDK-tagged)-Human histone deacetylase 8 (HDAC8), transcript variant 1
ORF Plasmid: tGFP
"NM_018486" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CDA07; CDLS5; HD8; HDACL1; KDAC8; MRXS6; RPD3; WTS |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC208390 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGCCGGAGGAACCGGCGGACAGTGGGCAGTCGCTGGTCCCGGTTTATATCTATAGTCCCGAGT ATGTCAGTATGTGTGACTCCCCGGCCAAGATCCCCAAACGGGCCAGTATGGTGCATTCTTTGATTGAAGC ATATGCACTGCATAAGCAAATGAGGATAGTTAAGCCTAAAGTGGCCTCCATGGAGGAGATGGCCACCTTC CACACTGATGCTTATCTGCAGCATCTCCAGAAGGTCAGCCAAGAGGGCGATGATGATCATCCGGACTCCA TAGAATATGGGCTAGGTTATGACTGCCCAGCCACTGAAGGGATATTTGACTATGCAGCAGCTATAGGAGG GGCTACGATCACAGCTGCCCAATGCCTGATTGACGGAATGTGCAAAGTAGCAATTAACTGGTCTGGAGGG TGGCATCATGCAAAGAAAGATGAAGCATCTGGTTTTTGTTATCTCAATGATGCTGTCCTGGGAATATTAC GATTGCGACGGAAATTTGAGCGTATTCTCTACGTGGATTTGGATCTGCACCATGGAGATGGTGTAGAAGA CGCATTCAGTTTCACCTCCAAAGTCATGACCGTGTCCCTGCACAAATTCTCCCCAGGATTTTTCCCAGGA ACAGGTGACGTGTCTGATGTTGGCCTAGGGAAGGGACGGTACTACAGTGTAAATGTGCCCATTCAGGATG GCATACAAGATGAAAAATATTACCAGATCTGTGAAAGTGTACTAAAGGAAGTATACCAAGCCTTTAATCC CAAAGCAGTGGTCTTACAGCTGGGAGCTGACACAATAGCTGGGGATCCCATGTGCTCCTTTAACATGACT CCAGTGGGAATTGGCAAGTGTCTTAAGTACATCCTTCAATGGCAGTTGGCAACACTCATTTTGGGAGGAG GAGGCTATAACCTTGCCAACACGGCTCGATGCTGGACATACTTGACCGGGGTCATCCTAGGGAAAACACT ATCCTCTGAGATCCCAGATCATGAGTTTTTCACAGCATATGGTCCTGATTATGTGCTGGAAATCACGCCA AGCTGCCGGCCAGACCGCAATGAGCCCCACCGAATCCAACAAATCCTCAACTACATCAAAGGGAATCTGA AGCATGTGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208390 protein sequence
Red=Cloning site Green=Tags(s) MEEPEEPADSGQSLVPVYIYSPEYVSMCDSPAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATF HTDAYLQHLQKVSQEGDDDHPDSIEYGLGYDCPATEGIFDYAAAIGGATITAAQCLIDGMCKVAINWSGG WHHAKKDEASGFCYLNDAVLGILRLRRKFERILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPG TGDVSDVGLGKGRYYSVNVPIQDGIQDEKYYQICESVLKEVYQAFNPKAVVLQLGADTIAGDPMCSFNMT PVGIGKCLKYILQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLSSEIPDHEFFTAYGPDYVLEITP SCRPDRNEPHRIQQILNYIKGNLKHVV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_018486 |
ORF Size | 1131 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018486.3 |
RefSeq Size | 2064 bp |
RefSeq ORF | 1134 bp |
Locus ID | 55869 |
UniProt ID | Q9BY41 |
Domains | Hist_deacetyl |
Protein Families | Druggable Genome, Transcription Factors |
MW | 41.7 kDa |
Gene Summary | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Isoform-specific characterization of class I histone deacetylases and their therapeutic modulation in pulmonary hypertension
,Chelladurai, P;Dabral, S;Basineni, SR;Chen, CN;Schmoranzer, M;Bender, N;Feld, C;Nötzold, RR;Dobreva, G;Wilhelm, J;Jungblut, B;Zhao, L;Bauer, UM;Seeger, W;Pullamsetti, SS;,
Sci Rep
,PubMed ID 32733053
[HDAC8]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC208390L1 | Lenti ORF clone of Human histone deacetylase 8 (HDAC8), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC208390L2 | Lenti ORF clone of Human histone deacetylase 8 (HDAC8), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
RC208390L3 | Lenti ORF clone of Human histone deacetylase 8 (HDAC8), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC208390L4 | Lenti ORF clone of Human histone deacetylase 8 (HDAC8), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
RG208390 | HDAC8 (tGFP-tagged) - Human histone deacetylase 8 (HDAC8), transcript variant 1 |
CNY 5,256.00 |
|
SC321829 | HDAC8 (untagged)-Human histone deacetylase 8 (HDAC8), transcript variant 1 |
CNY 3,656.00 |