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HDAC8 (NM_018486) Human Untagged Clone
CAT＃: SC321829
HDAC8 (untagged)-Human histone deacetylase 8 (HDAC8), transcript variant 1
CNY 3,656.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CDA07; CDLS5; HD8; HDACL1; KDAC8; MRXS6; RPD3; WTS |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_018486.1
GGAAGGTGGCTGCGGAACGGTTTTAAGCGGAAGATGGAGGAGCCGGAGGAACCGGCGGAC
AGTGGGCAGTCGCTGGTCCCGGTTTATATCTATAGTCCCGAGTATGTCAGTATGTGTGAC TCCCCGGCCAAGATCCCCAAACGGGCCAGTATGGTGCATTCTTTGATTGAAGCATATGCA CTGCATAAGCAAATGAGGATAGTTAAGCCTAAAGTGGCCTCCATGGAGGAGATGGCCACC TTCCACACTGATGCTTATCTGCAGCATCTCCAGAAGGTCAGCCAAGAGGGCGATGATGAT CATCCGGACTCCATAGAATATGGGCTAGGTTATGACTGCCCAGCCACTGAAGGGATATTT GACTATGCAGCAGCTATAGGAGGGGCTACGATCACAGCTGCCCAATGCCTGATTGACGGA ATGTGCAAAGTAGCAATTAACTGGTCTGGAGGGTGGCATCATGCAAAGAAAGATGAAGCA TCTGGTTTTTGTTATCTCAATGATGCTGTCCTGGGAATATTACGATTGCGACGGAAATTT GAGCGTATTCTCTACGTGGATTTGGATCTGCACCATGGAGATGGTGTAGAAGACGCATTC AGTTTCACCTCCAAAGTCATGACCGTGTCCCTGCACAAATTCTCCCCAGGATTTTTCCCA GGAACAGGTGACGTGTCTGATGTTGGCCTAGGGAAGGGACGGTACTACAGTGTAAATGTG CCCATTCAGGATGGCATACAAGATGAAAAATATTACCAGATCTGTGAAAGTGTACTAAAG GAAGTATACCAAGCCTTTAATCCCAAAGCAGTGGTCTTACAGCTGGGAGCTGACACAATA GCTGGGGATCCCATGTGCTCCTTTAACATGACTCCAGTGGGAATTGGCAAGTGTCTTAAG TACATCCTTCAATGGCAGTTGGCAACACTCATTTTGGGAGGAGGAGGCTATAACCTTGCC AACACGGCTCGATGCTGGACATACTTGACCGGGGTCATCCTAGGGAAAACACTATCCTCT GAGATCCCAGATCATGAGTTTTTCACAGCATATGGTCCTGATTATGTGCTGGAAATCACG CCAAGCTGCCGGCCAGACCGCAATGAGCCCCACCGAATCCAACAAATCCTCAACTACATC AAAGGGAATCTGAAGCATGTGGTCTAGTTGACAGAAAGAGATCAGGTTTCCAGAGCTGAG GAGTGGTGCCTATAATGAAGACAGCGTGTTTATGCAAGCAGTTTGTGGAATTTGTGACTG CAGGGAAAATTTGAAAGAAATTACTTCCTGAAAATTTCCAAGGGGCATCAAGTGGCAGCT GGCTTCCTGGGGTGAAGAGGCAGGCACCCCAGAGTCCTCAACTGGACCTAGGGGAAGAAG GAGATATCCCACATTTAAAGTTCTTATTTAAAAAAACACACACACACAAATGAAATTTTT AATCTTTGAAAATTATTTTTAAGCGAATTGGGGAGGGGAGTATTTTAATCATCTTAAATG AAACAGATCAGAAGCTGGATGAGAGCAGTCACCAGTTTGTAGGGCAGGAGGCAGCTGACA GGCAGGGTTTGGGCCTCAGGACCATCCAGGTGGAGCCCTGGGAGAGAGGGTACTGATCAG CAGACTGGGAGGTGGGGAGAAGTCCGCTGGTGTTGTTTTAGTGTTATATATCTTTGGTTT TTTTAATAAAATCTTTGAAAACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_018486 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018486.1, NP_060956.1 |
| RefSeq Size | 1743 bp |
| RefSeq ORF | 1134 bp |
| Locus ID | 55869 |
| UniProt ID | Q9BY41 |
| Domains | Hist_deacetyl |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: A downstream translational start codon is selected for this RefSeq based on a strong Kozak signal and transcript support. An upstream in-frame start codon is also present but has a weaker Kozak signal and sparse transcript support. Use of the upstream start codon would result in a protein that is 38 aa longer at the N-terminus. Leaky scanning by ribosomes may allow translation initiation at the downstream start codon, which is supported by 5'RACE experiments described in PubMed: 10922473 and encodes a protein with an N-terminus similar to other family members. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
HDAC8 and STAT3 repress BMF gene activity in colon cancer cells
,Kang, Y;Nian, H;Rajendran, P;Kim, E;Dashwood, WM;Pinto, JT;Boardman, LA;Thibodeau, SN;Limburg, PJ;Löhr, CV;Bisson, WH;Williams, DE;Ho, E;Dashwood, RH;,
Cell Death Dis
,PubMed ID 25321483
[HDAC8]
|
|
Histone deacetylase-3 interacts with ataxin-7 and is altered in a spinocerebellar ataxia type 7 mouse model
,null,
Molecular Neurodegeneration
,PubMed ID 24160175
[HDAC8]
|
Documents
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Resources
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| RC208390L2 | Lenti ORF clone of Human histone deacetylase 8 (HDAC8), transcript variant 1, mGFP tagged |
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| RC208390L3 | Lenti ORF clone of Human histone deacetylase 8 (HDAC8), transcript variant 1, Myc-DDK-tagged |
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| RC208390L4 | Lenti ORF clone of Human histone deacetylase 8 (HDAC8), transcript variant 1, mGFP tagged |
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| RG208390 | HDAC8 (tGFP-tagged) - Human histone deacetylase 8 (HDAC8), transcript variant 1 |
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