United States
Germany
Japan
United Kingdom
China
IKAP (IKBKAP) (NM_003640) Human Tagged ORF Clone
CAT＃: RC207686
IKBKAP (Myc-DDK-tagged)-Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein (IKBKAP)
ORF Plasmid: tGFP
"NM_003640" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 13,912.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DYS; FD; IKAP; IKBKAP; IKI3; TOT1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207686 representing NM_003640
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGAAATCTGAAATTATTTCGGACCCTGGAGTTCAGGGATATTCAAGGTCCAGGGAATCCTCAGTGCT TCTCTCTCCGAACTGAACAGGGGACGGTGCTCATTGGTTCAGAACATGGCCTGATAGAAGTAGACCCTGT CTCAAGAGAAGTGAAAAATGAAGTTTCTTTGGTGGCAGAAGGCTTTCTCCCAGAGGATGGAAGTGGCCGC ATTGTTGGTGTTCAGGACTTGCTGGATCAGGAGTCTGTGTGTGTGGCCACAGCCTCTGGAGACGTCATAC TCTGCAGTCTCAGCACACAACAGCTGGAGTGTGTTGGGAGTGTAGCCAGTGGTATCTCTGTTATGAGTTG GAGTCCTGACCAAGAGCTGGTGCTTCTTGCCACAGGTCAACAGACCCTGATTATGATGACAAAAGATTTT GAGCCAATCCTGGAGCAGCAGATCCATCAGGATGATTTTGGTGAAAGCAAGTTTATCACTGTTGGATGGG GTAGGAAGGAGACACAGTTCCATGGATCAGAAGGCAGACAAGCAGCTTTTCAGATGCAAATGCATGAGTC TGCTTTGCCCTGGGATGACCATAGACCACAAGTTACCTGGCGGGGGGATGGACAGTTTTTTGCTGTGAGT GTTGTTTGCCCAGAAACAGGGGCTCGGAAGGTCAGAGTGTGGAACCGAGAGTTTGCTTTGCAGTCAACCA GTGAGCCTGTGGCAGGACTGGGACCAGCCCTGGCTTGGAAACCCTCAGGCAGTTTGATTGCATCTACACA AGATAAACCCAACCAGCAGGATATTGTGTTTTTTGAGAAAAATGGACTCCTTCATGGACACTTTACACTT CCCTTCCTTAAAGATGAGGTTAAGGTAAATGACTTGCTCTGGAATGCAGATTCCTCTGTGCTTGCAGTCT GGCTGGAAGACCTTCAGAGAGAAGAAAGCTCCATTCCGAAAACCTGTGTTCAGCTCTGGACTGTTGGAAA CTATCACTGGTATCTCAAGCAAAGTTTATCCTTCAGCACCTGTGGGAAGAGCAAGATTGTGTCTCTGATG TGGGACCCTGTGACCCCATACCGGCTGCATGTTCTCTGTCAGGGCTGGCATTACCTCGCCTATGATTGGC ACTGGACGACTGACCGGAGCGTGGGAGATAATTCAAGTGACTTGTCCAATGTGGCTGTCATTGATGGAAA CAGGGTGTTGGTGACAGTCTTCCGGCAGACTGTGGTTCCGCCTCCCATGTGCACCTACCAACTGCTGTTC CCACACCCTGTGAATCAAGTCACATTCTTAGCACACCCTCAAAAGAGTAATGACCTTGCTGTTCTAGATG CCAGTAACCAGATTTCTGTTTATAAATGTGGTGATTGTCCAAGTGCTGACCCTACAGTGAAACTGGGAGC TGTGGGTGGAAGTGGATTTAAAGTTTGCCTTAGAACTCCTCATTTGGAAAAGAGATACAAAATCCAGTTT GAGAATAATGAAGATCAAGATGTAAACCCGCTGAAACTAGGCCTTCTCACTTGGATTGAAGAAGACGTCT TCCTGGCTGTAAGCCACAGTGAGTTCAGCCCCCGGTCTGTCATTCACCATTTGACTGCAGCTTCTTCTGA GATGGATGAAGAGCATGGACAGCTCAATGTCAGTTCATCTGCAGCGGTGGATGGGGTCATAATCAGTCTA TGTTGCAATTCCAAGACCAAGTCAGTAGTATTACAGCTGGCTGATGGCCAGATATTTAAGTACCTTTGGG AGTCACCTTCTCTGGCTATTAAACCATGGAAGAACTCTGGTGGATTTCCTGTTCGGTTTCCTTATCCATG CACCCAGACCGAATTGGCCATGATTGGAGAAGAGGAATGTGTCCTTGGTCTGACTGACAGGTGTCGCTTT TTCATCAATGACATTGAGGTTGCGTCAAATATCACGTCATTTGCAGTATATGATGAGTTTTTATTGTTGA CAACCCATTCCCATACCTGCCAGTGTTTTTGCCTGAGGGATGCTTCATTTAAAACATTACAGGCCGGCCT GAGCAGCAATCATGTGTCCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTCACTGTT GTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAAACTTAGAAGTTGTTCATCATCGAGCCC TGGTTTTAGCTCAGATTCGGAAGTGGTTGGACAAACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAA GCTGAGAATCAATCTCAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGTGGAAACCTTC ATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTTTTTACAGAATTGAAAGAAGAAGATGTCACGA AGACCATGTACCCTGCACCAGTTACCAGCAGTGTCTACCTGTCCAGGGATCCTGACGGGAATAAAATAGA CCTTGTCTGCGATGCTATGAGAGCAGTCATGGAGAGCATAAATCCTCATAAATACTGCCTATCCATACTT ACATCTCATGTAAAGAAGACAACCCCAGAACTGGAAATTGTACTGCAAAAAGTACACGAGCTTCAAGGAA ATGCTCCCTCTGATCCTGATGCTGTGAGTGCTGAAGAGGCCTTGAAATATTTGCTGCATCTGGTAGATGT TAATGAATTATATGATCATTCTCTTGGCACCTATGACTTTGATTTGGTCCTCATGGTAGCTGAGAAGTCA CAGAAGGATCCCAAAGAATATCTTCCATTTCTTAATACACTTAAGAAAATGGAAACTAATTATCAGCGGT TTACTATAGACAAATACTTGAAACGATATGAAAAAGCCATTGGCCACCTCAGCAAATGTGGACCTGAGTA CTTCCCAGAATGCTTAAACTTGATAAAAGATAAAAACTTGTATAACGAAGCTCTGAAGTTATATTCACCA AGCTCACAACAGTACCAGGATATCAGCATTGCTTATGGGGAGCACCTGATGCAGGAGCACATGTATGAGC CAGCGGGGCTCATGTTTGCCCGTTGCGGTGCCCACGAGAAAGCTCTCTCAGCCTTTCTCACATGTGGCAA CTGGAAGCAAGCCCTCTGTGTGGCAGCCCAGCTTAACTTTACCAAAGACCAGCTGGTGGGCCTCGGCAGA ACTCTGGCAGGAAAGCTGGTTGAGCAGAGGAAGCACATTGATGCGGCCATGGTTTTGGAAGAGTGTGCCC AGGATTATGAAGAAGCTGTGCTCTTGCTGTTAGAAGGAGCTGCCTGGGAAGAAGCTTTGAGGCTGGTATA CAAATATAACAGACTGGATATTATAGAAACCAACGTAAAGCCTTCCATTTTAGAAGCCCAGAAAAATTAT ATGGCATTTCTGGACTCTCAGACAGCCACATTCAGTCGCCACAAGAAACGTTTATTGGTAGTTCGAGAGC TCAAGGAGCAAGCCCAGCAGGCAGGTCTGGATGATGAGGTACCCCACGGGCAAGAGTCAGACCTCTTCTC TGAAACTAGCAGTGTCGTGAGTGGCAGTGAGATGAGTGGCAAATACTCCCATAGTAACTCCAGGATATCA GCGAGATCATCCAAGAATCGCCGAAAAGCGGAGCGGAAGAAGCACAGCCTCAAAGAAGGCAGTCCGCTGG AGGACCTGGCCCTCCTGGAGGCACTGAGTGAAGTGGTGCAGAACACTGAAAACCTGAAAGATGAAGTATA CCATATTTTAAAGGTACTCTTTCTCTTTGAGTTTGATGAACAAGGAAGGGAATTACAGAAGGCCTTTGAA GATACGCTGCAGTTGATGGAAAGGTCACTTCCAGAAATTTGGACTCTTACTTACCAGCAGAATTCAGCTA CCCCGGTTCTAGGTCCCAATTCTACTGCAAATAGTATCATGGCATCTTATCAGCAACAGAAGACTTCGGT TCCTGTTCTTGATGCTGAGCTTTTTATACCACCAAAGATCAACAGAAGAACCCAGTGGAAGCTGAGCCTG CTAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207686 representing NM_003640
Red=Cloning site Green=Tags(s) MRNLKLFRTLEFRDIQGPGNPQCFSLRTEQGTVLIGSEHGLIEVDPVSREVKNEVSLVAEGFLPEDGSGR IVGVQDLLDQESVCVATASGDVILCSLSTQQLECVGSVASGISVMSWSPDQELVLLATGQQTLIMMTKDF EPILEQQIHQDDFGESKFITVGWGRKETQFHGSEGRQAAFQMQMHESALPWDDHRPQVTWRGDGQFFAVS VVCPETGARKVRVWNREFALQSTSEPVAGLGPALAWKPSGSLIASTQDKPNQQDIVFFEKNGLLHGHFTL PFLKDEVKVNDLLWNADSSVLAVWLEDLQREESSIPKTCVQLWTVGNYHWYLKQSLSFSTCGKSKIVSLM WDPVTPYRLHVLCQGWHYLAYDWHWTTDRSVGDNSSDLSNVAVIDGNRVLVTVFRQTVVPPPMCTYQLLF PHPVNQVTFLAHPQKSNDLAVLDASNQISVYKCGDCPSADPTVKLGAVGGSGFKVCLRTPHLEKRYKIQF ENNEDQDVNPLKLGLLTWIEEDVFLAVSHSEFSPRSVIHHLTAASSEMDEEHGQLNVSSSAAVDGVIISL CCNSKTKSVVLQLADGQIFKYLWESPSLAIKPWKNSGGFPVRFPYPCTQTELAMIGEEECVLGLTDRCRF FINDIEVASNITSFAVYDEFLLLTTHSHTCQCFCLRDASFKTLQAGLSSNHVSHGEVLRKVERGSRIVTV VPQDTKLVLQMPRGNLEVVHHRALVLAQIRKWLDKLMFKEAFECMRKLRINLNLIYDHNPKVFLGNVETF IKQIDSVNHINLFFTELKEEDVTKTMYPAPVTSSVYLSRDPDGNKIDLVCDAMRAVMESINPHKYCLSIL TSHVKKTTPELEIVLQKVHELQGNAPSDPDAVSAEEALKYLLHLVDVNELYDHSLGTYDFDLVLMVAEKS QKDPKEYLPFLNTLKKMETNYQRFTIDKYLKRYEKAIGHLSKCGPEYFPECLNLIKDKNLYNEALKLYSP SSQQYQDISIAYGEHLMQEHMYEPAGLMFARCGAHEKALSAFLTCGNWKQALCVAAQLNFTKDQLVGLGR TLAGKLVEQRKHIDAAMVLEECAQDYEEAVLLLLEGAAWEEALRLVYKYNRLDIIETNVKPSILEAQKNY MAFLDSQTATFSRHKKRLLVVRELKEQAQQAGLDDEVPHGQESDLFSETSSVVSGSEMSGKYSHSNSRIS ARSSKNRRKAERKKHSLKEGSPLEDLALLEALSEVVQNTENLKDEVYHILKVLFLFEFDEQGRELQKAFE DTLQLMERSLPEIWTLTYQQNSATPVLGPNSTANSIMASYQQQKTSVPVLDAELFIPPKINRRTQWKLSL LD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003640 |
| ORF Size | 3996 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003640.5 |
| RefSeq Size | 5917 bp |
| RefSeq ORF | 3999 bp |
| Locus ID | 8518 |
| UniProt ID | O95163 |
| Domains | IKI3 |
| Protein Families | Druggable Genome |
| MW | 150.1 kDa |
| Gene Summary | The protein encoded by this gene is a scaffold protein and a regulator for three different kinases involved in proinflammatory signaling. The encoded protein can bind NF-kappa-B-inducing kinase and I-kappa-B kinases through separate domains and assemble them into an active kinase complex. Mutations in this gene have been associated with familial dysautonomia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207686L1 | Lenti ORF clone of Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein (IKBKAP), Myc-DDK-tagged |
CNY 16,312.00 |
|
| RC207686L2 | Lenti ORF clone of Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein (IKBKAP), mGFP tagged |
CNY 15,200.00 |
|
| RC207686L3 | Lenti ORF clone of Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein (IKBKAP), Myc-DDK-tagged |
CNY 15,200.00 |
|
| RC207686L4 | Lenti ORF clone of Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein (IKBKAP), mGFP tagged |
CNY 15,200.00 |
|
| RG207686 | IKBKAP (tGFP-tagged) - Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein (IKBKAP) |
CNY 15,512.00 |
|
| SC117829 | IKBKAP (untagged)-Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein (IKBKAP) |
CNY 13,920.00 |
