CIAO1 (NM_004804) Human Tagged ORF Clone

CAT#: RC207586

CIAO1 (Myc-DDK-tagged)-Human cytosolic iron-sulfur protein assembly 1 (CIAO1)

ORF Plasmid: DDK tGFP



  "NM_004804" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-CIAO1 Antibody
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CIA1; WDR39
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207586 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGACTCGCTGGTGCTGCTGGGCCGTGTCCCGGCGCACCCGGACTCCCGCTGCTGGTTCCTGGCCT
GGAACCCCGCGGGGACCCTGCTGGCCTCGTGCGGCGGCGACCGGAGAATCCGCATCTGGGGCACGGAGGG
TGACAGCTGGATCTGCAAGTCTGTCCTTTCTGAAGGCCACCAGCGCACCGTGCGGAAGGTAGCCTGGTCC
CCCTGCGGTAATTACCTGGCCTCTGCCAGCTTTGATGCTACCACTTGCATTTGGAAGAAGAACCAGGATG
ACTTTGAGTGTGTAACCACTCTCGAGGGCCATGAAAATGAGGTCAAGTCAGTGGCTTGGGCCCCATCTGG
CAACCTCCTGGCCACTTGCAGCCGAGATAAGAGCGTTTGGGTCTGGGAAGTTGATGAAGAGGATGAGTAT
GAATGTGTCAGTGTTCTCAACTCCCACACACAGGATGTCAAGCATGTGGTTTGGCACCCAAGTCAGGAGC
TCTTAGCTTCTGCCAGCTATGATGACACAGTGAAGCTGTACCGGGAGGAAGAGGATGACTGGGTATGCTG
TGCCACCCTTGAGGGCCATGAATCCACTGTGTGGAGCTTGGCCTTTGACCCGAGTGGCCAGCGCCTGGCG
TCTTGTAGTGATGACCGTACTGTGCGTATCTGGCGTCAGTATCTACCAGGCAATGAACAAGGGGTGGCAT
GCAGCGGCTCTGACCCCAGTTGGAAATGTATCTGTACTTTGTCCGGCTTCCACTCAAGGACCATTTATGA
CATTGCTTGGTGTCAGCTGACAGGGGCTCTGGCCACAGCTTGTGGGGATGACGCGATCCGCGTGTTTCAG
GAGGATCCCAACTCGGATCCACAGCAGCCCACCTTCTCCCTGACAGCCCACTTGCATCAGGCCCATTCCC
AGGATGTCAACTGTGTGGCCTGGAACCCCAAGGAGCCAGGGCTACTGGCCTCCTGCAGTGATGATGGGGA
GGTGGCCTTCTGGAAGTATCAGCGGCCTGAAGGCCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207586 protein sequence
Red=Cloning site Green=Tags(s)

MKDSLVLLGRVPAHPDSRCWFLAWNPAGTLLASCGGDRRIRIWGTEGDSWICKSVLSEGHQRTVRKVAWS
PCGNYLASASFDATTCIWKKNQDDFECVTTLEGHENEVKSVAWAPSGNLLATCSRDKSVWVWEVDEEDEY
ECVSVLNSHTQDVKHVVWHPSQELLASASYDDTVKLYREEEDDWVCCATLEGHESTVWSLAFDPSGQRLA
SCSDDRTVRIWRQYLPGNEQGVACSGSDPSWKCICTLSGFHSRTIYDIAWCQLTGALATACGDDAIRVFQ
EDPNSDPQQPTFSLTAHLHQAHSQDVNCVAWNPKEPGLLASCSDDGEVAFWKYQRPEGL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004804
ORF Size 1017 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004804.3
RefSeq Size 4053 bp
RefSeq ORF 1020 bp
Locus ID 9391
UniProt ID O76071
Domains WD40
Protein Families Transcription Factors
MW 37.8 kDa
Gene Summary Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins (PubMed:17937914, PubMed:23891004). As a CIA complex component, interacts specifically with CIAO2A or CIAO2B and MMS19 to assist different branches of iron-sulfur protein assembly, depending of its interactors. The complex CIAO1:CIAO2B:MMS19 binds to and facilitates the assembly of most cytosolic-nuclear Fe/S proteins. CIAO1:CIAO2A specifically matures ACO1 and stabilizes IREB2 (PubMed:23891004). Seems to specifically modulate the transactivation activity of WT1 (PubMed:9556563). As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation (PubMed:20797633).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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