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CIAO1 (NM_004804) Human Tagged ORF Clone
CAT＃: RC207586
CIAO1 (Myc-DDK-tagged)-Human cytosolic iron-sulfur protein assembly 1 (CIAO1)
ORF Plasmid: tGFP
"NM_004804" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CIA1; WDR39 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207586 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGACTCGCTGGTGCTGCTGGGCCGTGTCCCGGCGCACCCGGACTCCCGCTGCTGGTTCCTGGCCT GGAACCCCGCGGGGACCCTGCTGGCCTCGTGCGGCGGCGACCGGAGAATCCGCATCTGGGGCACGGAGGG TGACAGCTGGATCTGCAAGTCTGTCCTTTCTGAAGGCCACCAGCGCACCGTGCGGAAGGTAGCCTGGTCC CCCTGCGGTAATTACCTGGCCTCTGCCAGCTTTGATGCTACCACTTGCATTTGGAAGAAGAACCAGGATG ACTTTGAGTGTGTAACCACTCTCGAGGGCCATGAAAATGAGGTCAAGTCAGTGGCTTGGGCCCCATCTGG CAACCTCCTGGCCACTTGCAGCCGAGATAAGAGCGTTTGGGTCTGGGAAGTTGATGAAGAGGATGAGTAT GAATGTGTCAGTGTTCTCAACTCCCACACACAGGATGTCAAGCATGTGGTTTGGCACCCAAGTCAGGAGC TCTTAGCTTCTGCCAGCTATGATGACACAGTGAAGCTGTACCGGGAGGAAGAGGATGACTGGGTATGCTG TGCCACCCTTGAGGGCCATGAATCCACTGTGTGGAGCTTGGCCTTTGACCCGAGTGGCCAGCGCCTGGCG TCTTGTAGTGATGACCGTACTGTGCGTATCTGGCGTCAGTATCTACCAGGCAATGAACAAGGGGTGGCAT GCAGCGGCTCTGACCCCAGTTGGAAATGTATCTGTACTTTGTCCGGCTTCCACTCAAGGACCATTTATGA CATTGCTTGGTGTCAGCTGACAGGGGCTCTGGCCACAGCTTGTGGGGATGACGCGATCCGCGTGTTTCAG GAGGATCCCAACTCGGATCCACAGCAGCCCACCTTCTCCCTGACAGCCCACTTGCATCAGGCCCATTCCC AGGATGTCAACTGTGTGGCCTGGAACCCCAAGGAGCCAGGGCTACTGGCCTCCTGCAGTGATGATGGGGA GGTGGCCTTCTGGAAGTATCAGCGGCCTGAAGGCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207586 protein sequence
Red=Cloning site Green=Tags(s) MKDSLVLLGRVPAHPDSRCWFLAWNPAGTLLASCGGDRRIRIWGTEGDSWICKSVLSEGHQRTVRKVAWS PCGNYLASASFDATTCIWKKNQDDFECVTTLEGHENEVKSVAWAPSGNLLATCSRDKSVWVWEVDEEDEY ECVSVLNSHTQDVKHVVWHPSQELLASASYDDTVKLYREEEDDWVCCATLEGHESTVWSLAFDPSGQRLA SCSDDRTVRIWRQYLPGNEQGVACSGSDPSWKCICTLSGFHSRTIYDIAWCQLTGALATACGDDAIRVFQ EDPNSDPQQPTFSLTAHLHQAHSQDVNCVAWNPKEPGLLASCSDDGEVAFWKYQRPEGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004804 |
| ORF Size | 1017 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004804.3 |
| RefSeq Size | 4053 bp |
| RefSeq ORF | 1020 bp |
| Locus ID | 9391 |
| UniProt ID | O76071 |
| Domains | WD40 |
| Protein Families | Transcription Factors |
| MW | 37.8 kDa |
| Gene Summary | Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins (PubMed:17937914, PubMed:23891004). As a CIA complex component, interacts specifically with CIAO2A or CIAO2B and MMS19 to assist different branches of iron-sulfur protein assembly, depending of its interactors. The complex CIAO1:CIAO2B:MMS19 binds to and facilitates the assembly of most cytosolic-nuclear Fe/S proteins. CIAO1:CIAO2A specifically matures ACO1 and stabilizes IREB2 (PubMed:23891004). Seems to specifically modulate the transactivation activity of WT1 (PubMed:9556563). As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation (PubMed:20797633).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207586L1 | Lenti ORF clone of Human cytosolic iron-sulfur protein assembly 1 (CIAO1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC207586L2 | Lenti ORF clone of Human cytosolic iron-sulfur protein assembly 1 (CIAO1), mGFP tagged |
CNY 5,890.00 |
|
| RC207586L3 | Lenti ORF clone of Human cytosolic iron-sulfur protein assembly 1 (CIAO1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207586L4 | Lenti ORF clone of Human cytosolic iron-sulfur protein assembly 1 (CIAO1), mGFP tagged |
CNY 5,890.00 |
|
| RG207586 | CIAO1 (tGFP-tagged) - Human cytosolic iron-sulfur protein assembly 1 (CIAO1) |
CNY 5,256.00 |
|
| SC321723 | CIAO1 (untagged)-Human cytosolic iron-sulfur protein assembly 1 (CIAO1) |
CNY 3,656.00 |
