ADAR1 (ADAR) (NM_001111) Human Tagged ORF Clone

CAT＃: RC207522

TrueORF^®

ADAR (Myc-DDK-tagged)-Human adenosine deaminase, RNA-specific (ADAR), transcript variant 1

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_001111" in other vectors (6)

Need custom modification / cloning service?
Get a free quote

CNY 12,800.00

货期*

现货

规格

Cited in 3 publications.

Product images

经常一起买 (5)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

TA308833

Rabbit polyclonal antibody to Adenosine Deaminase (adenosine deaminase, RNA-specific)

CNY 6,281.00

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ADAR1; AGS6; DRADA; DSH; DSRAD; G1P1; IFI-4; IFI4; K88DSRBP; P136
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_001111
ORF Size	3678 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001111.5
RefSeq Size	6692 bp
RefSeq ORF	3681 bp
Locus ID	103
UniProt ID	P55265
Domains	z-alpha, DSRM, A_deamin
Protein Families	Druggable Genome
Protein Pathways	Cytosolic DNA-sensing pathway
MW	136 kDa
Gene Summary	This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (3)

The use of this cDNA Clones has been cited in the following citations:
RNA modifications can affect RNase H1-mediated PS-ASO activity ,null, Molecular Therapy. Nucleic Acids ,PubMed ID 35664704 [ADAR1]
The Role of ADAR1 and ADAR2 in the Regulation of miRNA-21 in Idiopathic Pulmonary Fibrosis ,Díaz-Piña, G;Ordoñez-Razo, RM;Montes, E;Páramo, I;Becerril, C;Salgado, A;Santibañez-Salgado, JA;Maldonado, M;Ruiz, V;, Lung ,PubMed ID 29637273 [ADAR1]
PTBP1 induces ADAR1 p110 isoform expression through IRES-like dependent translation control and influences cell proliferation in gliomas. ,null, Cellular and molecular life sciences : CMLS ,PubMed ID 26047657 [ADAR1]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC207522L1	Lenti ORF clone of Human adenosine deaminase, RNA-specific (ADAR), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 15,200.00
RC207522L2	Lenti ORF clone of Human adenosine deaminase, RNA-specific (ADAR), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 11,500.00
RC207522L3	Lenti ORF clone of Human adenosine deaminase, RNA-specific (ADAR), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 15,200.00
RC207522L4	Lenti ORF clone of Human adenosine deaminase, RNA-specific (ADAR), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 15,200.00
RG207522	ADAR (tGFP-tagged) - Human adenosine deaminase, RNA-specific (ADAR), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 14,400.00
SC119438	ADAR (untagged)-Human adenosine deaminase, RNA-specific (ADAR), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 12,816.00