MIF4GD (NM_020679) Human Tagged ORF Clone
CAT#: RC207489
MIF4GD (Myc-DDK-tagged)-Human MIF4G domain containing (MIF4GD)
ORF Plasmid: tGFP
"NM_020679" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
Cited in 1 publication. |
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AD023; MIFD; SLIP1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207489 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGAGCCCAGTAGAGAGGAGTATAAAATCCAGTCCTTTGATGCAGAGACCCAGCAGCTGCTGAAGA CAGCACTCAAAGTTGCCTGCTTTGAGACAGAAGATGGAGAATATTCCGTTTGCCAGAGGAGCTATAGTAA TTGCTCTCGCCTGATGCCTTCCCGCTGTAACACGCAGTACAGAGATCCGGGTGCTGTGGACTTGGAGAAA GTGGCCAATGTGATTGTGGACCATTCTCTGCAGGACTGTGTGTTCAGCAAGGAAGCAGGACGCATGTGCT ACGCCATCATTCAGGCAGAGAGTAAACAAGCAGGCCAGAGTGTCTTCCGACGTGGACTCCTCAACCGGCT GCAGCAGGAGTACCAGGCTCGGGAGCAGCTGCGAGCACGCTCCCTGCAGGGCTGGGTCTGCTATGTCACC TTTATCTGCAACATCTTTGACTACCTGAGGGTGAACAACATGCCCATGATGGCCCTGGTGAACCCTGTCT ATGACTGCCTCTTCCGGCTGGCCCAGCCAGACAGTTTGAGCAAGGAGGAGGAGGTGGACTGTTTGGTGCT GCAGCTGCACCGGGTTGGGGAGCAGCTGGAGAAAATGAATGGGCAGCGCATGGATGAGCTCTTTGTGCTG ATCCGGGATGGCTTCCTGCTCCCAACTGGCCTCAGCTCCCTGGCCCAGCTGCTGCTGCTGGAGATCATTG AGTTCCGGGCGGCCGGCTGGAAGACAACGCCAGCTGCCCACAAGTATTACTACAGCGAAGTCTCCGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207489 protein sequence
Red=Cloning site Green=Tags(s) MGEPSREEYKIQSFDAETQQLLKTALKVACFETEDGEYSVCQRSYSNCSRLMPSRCNTQYRDPGAVDLEK VANVIVDHSLQDCVFSKEAGRMCYAIIQAESKQAGQSVFRRGLLNRLQQEYQAREQLRARSLQGWVCYVT FICNIFDYLRVNNMPMMALVNPVYDCLFRLAQPDSLSKEEEVDCLVLQLHRVGEQLEKMNGQRMDELFVL IRDGFLLPTGLSSLAQLLLLEIIEFRAAGWKTTPAAHKYYYSEVSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020679 |
ORF Size | 768 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020679.3 |
RefSeq Size | 1441 bp |
RefSeq ORF | 771 bp |
Locus ID | 57409 |
UniProt ID | A9UHW6 |
Domains | MIF4G, MA3 |
MW | 29.4 kDa |
Gene Summary | This gene encodes a protein which interacts with the N-terminus of the stem-loop binding protein (SLBP) and the 3' end of histone mRNA. This interaction facilitates the activation of histone mRNA translation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
INT6 interacts with MIF4GD/SLIP1 and is necessary for efficient histone mRNA translation
,null,
RNA
,PubMed ID 22532700
[MIF4GD]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207489L1 | Lenti ORF clone of Human MIF4G domain containing (MIF4GD), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC207489L2 | Lenti ORF clone of Human MIF4G domain containing (MIF4GD), mGFP tagged |
CNY 5,890.00 |
|
RC207489L3 | Lenti ORF clone of Human MIF4G domain containing (MIF4GD), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC207489L4 | Lenti ORF clone of Human MIF4G domain containing (MIF4GD), mGFP tagged |
CNY 5,890.00 |
|
RG207489 | MIF4GD (tGFP-tagged) - Human MIF4G domain containing (MIF4GD) |
CNY 4,000.00 |
|
SC322663 | MIF4GD (untagged)-Human MIF4G domain containing (MIF4GD) |
CNY 2,400.00 |