MIF4GD (NM_020679) Human Tagged ORF Clone

CAT#: RG207489

  • TrueORF®

MIF4GD (tGFP-tagged) - Human MIF4G domain containing (MIF4GD)

ORF Plasmid: DDK tGFP



  "NM_020679" in other vectors (6)

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CNY 4,000.00

CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


MIF4GD mouse monoclonal antibody, clone OTI4C11 (formerly 4C11)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms AD023; MIFD; SLIP1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG207489 representing NM_020679
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGAGCCCAGTAGAGAGGAGTATAAAATCCAGTCCTTTGATGCAGAGACCCAGCAGCTGCTGAAGA
CAGCACTCAAAGTTGCCTGCTTTGAGACAGAAGATGGAGAATATTCCGTTTGCCAGAGGAGCTATAGTAA
TTGCTCTCGCCTGATGCCTTCCCGCTGTAACACGCAGTACAGAGATCCGGGTGCTGTGGACTTGGAGAAA
GTGGCCAATGTGATTGTGGACCATTCTCTGCAGGACTGTGTGTTCAGCAAGGAAGCAGGACGCATGTGCT
ACGCCATCATTCAGGCAGAGAGTAAACAAGCAGGCCAGAGTGTCTTCCGACGTGGACTCCTCAACCGGCT
GCAGCAGGAGTACCAGGCTCGGGAGCAGCTGCGAGCACGCTCCCTGCAGGGCTGGGTCTGCTATGTCACC
TTTATCTGCAACATCTTTGACTACCTGAGGGTGAACAACATGCCCATGATGGCCCTGGTGAACCCTGTCT
ATGACTGCCTCTTCCGGCTGGCCCAGCCAGACAGTTTGAGCAAGGAGGAGGAGGTGGACTGTTTGGTGCT
GCAGCTGCACCGGGTTGGGGAGCAGCTGGAGAAAATGAATGGGCAGCGCATGGATGAGCTCTTTGTGCTG
ATCCGGGATGGCTTCCTGCTCCCAACTGGCCTCAGCTCCCTGGCCCAGCTGCTGCTGCTGGAGATCATTG
AGTTCCGGGCGGCCGGCTGGAAGACAACGCCAGCTGCCCACAAGTATTACTACAGCGAAGTCTCCGAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG207489 representing NM_020679
Red=Cloning site Green=Tags(s)

MGEPSREEYKIQSFDAETQQLLKTALKVACFETEDGEYSVCQRSYSNCSRLMPSRCNTQYRDPGAVDLEK
VANVIVDHSLQDCVFSKEAGRMCYAIIQAESKQAGQSVFRRGLLNRLQQEYQAREQLRARSLQGWVCYVT
FICNIFDYLRVNNMPMMALVNPVYDCLFRLAQPDSLSKEEEVDCLVLQLHRVGEQLEKMNGQRMDELFVL
IRDGFLLPTGLSSLAQLLLLEIIEFRAAGWKTTPAAHKYYYSEVSD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_020679
ORF Size 768 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_020679.3
RefSeq Size 1433 bp
RefSeq ORF 771 bp
Locus ID 57409
UniProt ID A9UHW6
Domains MIF4G, MA3
Gene Summary This gene encodes a protein which interacts with the N-terminus of the stem-loop binding protein (SLBP) and the 3' end of histone mRNA. This interaction facilitates the activation of histone mRNA translation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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