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Hsp105 (HSPH1) (NM_006644) Human Tagged ORF Clone
CAT＃: RC207102
HSPH1 (Myc-DDK-tagged)-Human heat shock 105kDa/110kDa protein 1 (HSPH1)
ORF Plasmid: tGFP
"NM_006644" in other vectors (6)
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CNY 6,288.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HSP105; HSP105A; HSP105B; NY-CO-25 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207102 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGTGGTGGGGTTGGACGTGGGCTCGCAGAGCTGCTACATCGCGGTAGCCCGGGCCGGGGGCATCG AGACCATCGCCAATGAGTTCAGCGACCGGTGCACCCCGTCAGTCATATCATTTGGATCAAAAAATAGAAC AATCGGAGTTGCAGCCAAAAATCAGCAAATCACTCATGCAAACAATACGGTGTCTAACTTCAAAAGATTT CATGGCCGAGCATTCAATGACCCCTTCATTCAAAAGGAGAAGGAAAACTTGAGTTACGATTTGGTTCCAT TGAAAAATGGTGGAGTTGGAATAAAGGTAATGTACATGGGTGAAGAACATCTATTTAGTGTGGAGCAGAT AACAGCCATGTTGTTGACTAAGCTGAAGGAAACTGCTGAAAACAGCCTCAAGAAACCAGTAACAGATTGT GTTATTTCAGTCCCCTCCTTCTTTACAGATGCTGAGAGGCGATCTGTGTTAGATGCTGCACAGATTGTTG GCCTAAACTGTTTAAGACTTATGAATGACATGACAGCTGTTGCTTTGAATTACGGAATTTATAAGCAGGA TCTCCCAAGCCTGGATGAGAAACCTCGGATAGTGGTTTTTGTTGATATGGGACATTCAGCTTTTCAAGTG TCTGCTTGTGCTTTTAACAAGGGAAAATTGAAGGTACTGGGAACAGCTTTTGATCCTTTCTTAGGAGGAA AAAACTTCGATGAAAAGTTAGTGGAACATTTTTGTGCAGAATTTAAAACTAAGTACAAGTTGGATGCAAA ATCCAAAATACGAGCACTCCTACGTCTGTATCAGGAATGTGAAAAACTGAAAAAGCTAATGAGCTCTAAC AGCACAGACCTTCCACTGAATATCGAATGCTTTATGAATGATAAAGATGTTTCCGGAAAGATGAACAGGT CACAATTTGAAGAACTCTGTGCTGAACTTCTGCAAAAGATAGAAGTACCCCTTTATTCACTGTTGGAACA AACTCATCTCAAAGTAGAAGATGTGAGTGCAGTTGAGATTGTTGGAGGCGCTACACGAATTCCAGCTGTG AAGGAAAGAATTGCCAAATTCTTTGGAAAAGATATTAGCACAACACTCAATGCAGATGAAGCAGTAGCCA GAGGATGTGCATTACAGTGTGCAATACTTTCCCCGGCATTTAAAGTTAGAGAATTTTCCGTCACAGATGC AGTTCCTTTTCCAATATCTCTGATCTGGAACCATGATTCAGAAGATACTGAAGGTGTTCATGAAGTCTTT AGTCGAAACCATGCTGCTCCTTTCTCCAAAGTTCTCACCTTTCTGAGAAGGGGGCCTTTTGAGCTAGAAG CTTTCTATTCTGATCCCCAAGGAGTTCCATATCCAGAAGCAAAAATAGGCCGCTTTGTAGTTCAGAATGT TTCTGCACAGAAAGATGGAGAAAAATCTAGAGTAAAAGTCAAAGTGCGAGTCAACACCCATGGCATTTTC ACCATCTCTACGGCATCTATGGTGGAGAAAGTCCCAACTGAGGAGAATGAAATGTCTTCTGAAGCTGACA TGGAGTGTCTGAATCAGAGACCACCAGAAAACCCAGACACTGATAAAAATGTCCAGCAAGACAACAGTGA AGCTGGAACACAGCCCCAGGTACAAACTGATGCTCAACAAACCTCACAGTCTCCCCCTTCACCTGAACTT ACCTCAGAAGAAAACAAAATCCCAGATGCTGACAAAGCAAATGAAAAAAAAGTTGACCAGCCTCCAGAAG CTAAAAAGCCCAAAATAAAGGTGGTGAATGTTGAGCTGCCTATTGAAGCCAACTTGGTCTGGCAGTTAGG GAAAGACCTTCTTAACATGTATATTGAGACAGAGGGTAAGATGATAATGCAAGATAAATTGGAAAAAGAA AGGAATGATGCTAAAAATGCAGTTGAGGAATATGTGTATGAGTTCAGAGACAAGCTGTGTGGACCATATG AAAAATTTATATGTGAGCAGGATCATCAAAATTTTTTGAGACTCCTCACAGAAACTGAAGACTGGCTGTA TGAAGAAGGAGAGGACCAAGCTAAACAAGCATATGTTGACAAGTTGGAAGAATTAATGAAAATTGGCACT CCAGTTAAAGTTCGGTTTCAGGAAGCTGAAGAACGGCCAAAAATGTTTGAAGAACTAGGACAGAGGCTGC AGCATTATGCCAAGATAGCAGCTGACTTCAGAAATAAGGATGAGAAATACAACCATATTGATGAGTCTGA AATGAAAAAAGTGGAGAAGTCTGTTAATGAAGTGATGGAATGGATGAATAATGTCATGAATGCTCAGGCT AAAAAGAGTCTTGATCAGGATCCAGTTGTACGTGCTCAGGAAATTAAAACAAAAATCAAGGAATTGAACA ACACATGTGAACCCGTTGTAACACAACCGAAACCAAAAATTGAATCACCCAAACTGGAAAGAACTCCAAA TGGCCCAAATATTGATAAAAAGGAAGAAGATTTAGAAGACAAAAACAATTTTGGTGCTGAACCTCCACAT CAGAATGGTGAATGTTACCCTAATGAGAAAAATTCTGTTAATATGGACTTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207102 protein sequence
Red=Cloning site Green=Tags(s) MSVVGLDVGSQSCYIAVARAGGIETIANEFSDRCTPSVISFGSKNRTIGVAAKNQQITHANNTVSNFKRF HGRAFNDPFIQKEKENLSYDLVPLKNGGVGIKVMYMGEEHLFSVEQITAMLLTKLKETAENSLKKPVTDC VISVPSFFTDAERRSVLDAAQIVGLNCLRLMNDMTAVALNYGIYKQDLPSLDEKPRIVVFVDMGHSAFQV SACAFNKGKLKVLGTAFDPFLGGKNFDEKLVEHFCAEFKTKYKLDAKSKIRALLRLYQECEKLKKLMSSN STDLPLNIECFMNDKDVSGKMNRSQFEELCAELLQKIEVPLYSLLEQTHLKVEDVSAVEIVGGATRIPAV KERIAKFFGKDISTTLNADEAVARGCALQCAILSPAFKVREFSVTDAVPFPISLIWNHDSEDTEGVHEVF SRNHAAPFSKVLTFLRRGPFELEAFYSDPQGVPYPEAKIGRFVVQNVSAQKDGEKSRVKVKVRVNTHGIF TISTASMVEKVPTEENEMSSEADMECLNQRPPENPDTDKNVQQDNSEAGTQPQVQTDAQQTSQSPPSPEL TSEENKIPDADKANEKKVDQPPEAKKPKIKVVNVELPIEANLVWQLGKDLLNMYIETEGKMIMQDKLEKE RNDAKNAVEEYVYEFRDKLCGPYEKFICEQDHQNFLRLLTETEDWLYEEGEDQAKQAYVDKLEELMKIGT PVKVRFQEAEERPKMFEELGQRLQHYAKIAADFRNKDEKYNHIDESEMKKVEKSVNEVMEWMNNVMNAQA KKSLDQDPVVRAQEIKTKIKELNNTCEPVVTQPKPKIESPKLERTPNGPNIDKKEEDLEDKNNFGAEPPH QNGECYPNEKNSVNMDLD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006644 |
| ORF Size | 2574 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006644.4 |
| RefSeq Size | 5360 bp |
| RefSeq ORF | 2577 bp |
| Locus ID | 10808 |
| UniProt ID | Q92598 |
| Domains | HSP70 |
| Protein Families | Stem cell - Pluripotency |
| MW | 96.9 kDa |
| Gene Summary | This gene encodes a member of the heat shock protein 70 family of proteins. The encoded protein functions as a nucleotide exchange factor for the molecular chaperone heat shock cognate 71 kDa protein (Hsc70). In addition, this protein plays a distinct but related role as a holdase that inhibits the aggregation of misfolded proteins, including the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Elevated expression of this protein has been observed in numerous human cancers. [provided by RefSeq, Mar 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207102L1 | Lenti ORF clone of Human heat shock 105kDa/110kDa protein 1 (HSPH1), Myc-DDK-tagged |
CNY 8,688.00 |
|
| RC207102L2 | Lenti ORF clone of Human heat shock 105kDa/110kDa protein 1 (HSPH1), mGFP tagged |
CNY 5,890.00 |
|
| RC207102L3 | Lenti ORF clone of Human heat shock 105kDa/110kDa protein 1 (HSPH1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207102L4 | Lenti ORF clone of Human heat shock 105kDa/110kDa protein 1 (HSPH1), mGFP tagged |
CNY 5,890.00 |
|
| RG207102 | HSPH1 (tGFP-tagged) - Human heat shock 105kDa/110kDa protein 1 (HSPH1) |
CNY 7,888.00 |
|
| SC115932 | HSPH1 (untagged)-Human heat shock 105kDa/110kDa protein 1 (HSPH1) |
CNY 6,288.00 |
