Beta TRCP (BTRC) (NM_033637) Human Tagged ORF Clone
CAT#: RC207025
- TrueORF®
BTRC (Myc-DDK-tagged)-Human beta-transducin repeat containing (BTRC), transcript variant 1
ORF Plasmid: tGFP
"NM_033637" in other vectors (6)
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CNY 4,504.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A; FBXW1; FBXW1A; FWD1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207025 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCCGGCCGAGGCGGTGCTGCAAGAGAAGGCACTCAAGTTTATGTGCTCTATGCCCAGGTCTCTGT GGCTGGGCTGCTCCAGCCTGGCGGACAGCATGCCTTCGCTGCGATGCCTGTATAACCCAGGGACTGGCGC ACTCACAGCTTTCCAGAATTCCTCAGAGAGAGAAGACTGTAATAATGGCGAACCCCCTAGGAAGATAATA CCAGAGAAGAATTCACTTAGACAGACATACAACAGCTGTGCCAGACTCTGCTTAAACCAAGAAACAGTAT GTTTAGCAAGCACTGCTATGAAGACTGAGAATTGTGTGGCCAAAACAAAACTTGCCAATGGCACTTCCAG TATGATTGTGCCCAAGCAACGGAAACTCTCAGCAAGCTATGAAAAGGAAAAGGAACTGTGTGTCAAATAC TTTGAGCAGTGGTCAGAGTCAGATCAAGTGGAATTTGTGGAACATCTTATATCCCAAATGTGTCATTACC AACATGGGCACATAAACTCGTATCTTAAACCTATGTTGCAGAGAGATTTCATAACTGCTCTGCCAGCTCG GGGATTGGATCATATTGCTGAGAACATTCTGTCATACCTGGATGCCAAATCACTATGTGCTGCTGAACTT GTGTGCAAGGAATGGTACCGAGTGACCTCTGATGGCATGCTGTGGAAGAAGCTTATCGAGAGAATGGTCA GGACAGATTCTCTGTGGAGAGGCCTGGCAGAACGAAGAGGATGGGGACAGTATTTATTCAAAAACAAACC TCCTGACGGGAATGCTCCTCCCAACTCTTTTTATAGAGCACTTTATCCTAAAATTATACAAGACATTGAG ACAATAGAATCTAATTGGAGATGTGGAAGACATAGTTTACAGAGAATTCACTGCCGAAGTGAAACAAGCA AAGGAGTTTACTGTTTACAGTATGATGATCAGAAAATAGTAAGCGGCCTTCGAGACAACACAATCAAGAT CTGGGATAAAAACACATTGGAATGCAAGCGAATTCTCACAGGCCATACAGGTTCAGTCCTCTGTCTCCAG TATGATGAGAGAGTGATCATAACAGGATCATCGGATTCCACGGTCAGAGTGTGGGATGTAAATACAGGTG AAATGCTAAACACGTTGATTCACCATTGTGAAGCAGTTCTGCACTTGCGTTTCAATAATGGCATGATGGT GACCTGCTCCAAAGATCGTTCCATTGCTGTATGGGATATGGCCTCCCCAACTGACATTACCCTCCGGAGG GTGCTGGTCGGACACCGAGCTGCTGTCAATGTTGTAGACTTTGATGACAAGTACATTGTTTCTGCATCTG GGGATAGAACTATAAAGGTATGGAACACAAGTACTTGTGAATTTGTAAGGACCTTAAATGGACACAAACG AGGCATTGCCTGTTTGCAGTACAGGGACAGGCTGGTAGTGAGTGGCTCATCTGACAACACTATCAGATTA TGGGACATAGAATGTGGTGCATGTTTACGAGTGTTAGAAGGCCATGAGGAATTGGTGCGTTGTATTCGAT TTGATAACAAGAGGATAGTCAGTGGGGCCTATGATGGAAAAATTAAAGTGTGGGATCTTGTGGCTGCTTT GGACCCCCGTGCTCCTGCAGGGACACTCTGTCTACGGACCCTTGTGGAGCATTCCGGAAGAGTTTTTCGA CTACAGTTTGATGAATTCCAGATTGTCAGTAGTTCACATGATGACACAATCCTCATCTGGGACTTCCTAA ATGATCCAGCTGCCCAAGCTGAACCCCCCCGTTCCCCTTCTCGAACATACACCTACATCTCCAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207025 protein sequence
Red=Cloning site Green=Tags(s) MDPAEAVLQEKALKFMCSMPRSLWLGCSSLADSMPSLRCLYNPGTGALTAFQNSSEREDCNNGEPPRKII PEKNSLRQTYNSCARLCLNQETVCLASTAMKTENCVAKTKLANGTSSMIVPKQRKLSASYEKEKELCVKY FEQWSESDQVEFVEHLISQMCHYQHGHINSYLKPMLQRDFITALPARGLDHIAENILSYLDAKSLCAAEL VCKEWYRVTSDGMLWKKLIERMVRTDSLWRGLAERRGWGQYLFKNKPPDGNAPPNSFYRALYPKIIQDIE TIESNWRCGRHSLQRIHCRSETSKGVYCLQYDDQKIVSGLRDNTIKIWDKNTLECKRILTGHTGSVLCLQ YDERVIITGSSDSTVRVWDVNTGEMLNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMASPTDITLRR VLVGHRAAVNVVDFDDKYIVSASGDRTIKVWNTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRL WDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLVAALDPRAPAGTLCLRTLVEHSGRVFR LQFDEFQIVSSSHDDTILIWDFLNDPAAQAEPPRSPSRTYTYISR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_033637 |
ORF Size | 1815 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_033637.4 |
RefSeq Size | 6180 bp |
RefSeq ORF | 1818 bp |
Locus ID | 8945 |
UniProt ID | Q9Y297 |
Domains | WD40, F-box |
Protein Families | Druggable Genome |
Protein Pathways | Hedgehog signaling pathway, Oocyte meiosis, Ubiquitin mediated proteolysis, Wnt signaling pathway |
MW | 68.9 kDa |
Gene Summary | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6. [provided by RefSeq, Mar 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207025L1 | Lenti ORF clone of Human beta-transducin repeat containing (BTRC), transcript variant 1, Myc-DDK-tagged |
CNY 6,904.00 |
|
RC207025L2 | Lenti ORF clone of Human beta-transducin repeat containing (BTRC), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC207025L3 | Lenti ORF clone of Human beta-transducin repeat containing (BTRC), transcript variant 1, Myc-DDK-tagged |
CNY 6,904.00 |
|
RC207025L4 | Lenti ORF clone of Human beta-transducin repeat containing (BTRC), transcript variant 1, mGFP tagged |
CNY 6,904.00 |
|
RG207025 | BTRC (tGFP-tagged) - Human beta-transducin repeat containing (BTRC), transcript variant 1 |
CNY 6,104.00 |
|
SC110015 | BTRC (untagged)-Human beta-transducin repeat containing (BTRC), transcript variant 1 |
CNY 4,512.00 |
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