United States
Germany
Japan
United Kingdom
China
BBS10 (NM_024685) Human Tagged ORF Clone
CAT＃: RC205501
BBS10 (Myc-DDK-tagged)-Human Bardet-Biedl syndrome 10 (BBS10)
ORF Plasmid: tGFP
"NM_024685" in other vectors (5)
Need custom modification / cloning service?
Get a free quote
CNY 5,296.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C12orf58 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205501 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTAAGTTCTATGGCCGCTGCAGGGTCTGTGAAGGCGGCGTTGCAGGTGGCCGAGGTGCTGGAAGCCA TCGTGAGCTGCTGCGTGGGGCCCGAGGGACGGCAAGTTTTGTGTACGAAGCCCACTGGCGAGGTGCTTCT CAGCCGGAATGGAGGCCGCCTCCTGGAGGCGCTACACTTAGAGCATCCCATAGCCAGGATGATAGTGGAC TGTGTTTCCAGTCATCTCAAAAAAACAGGAGATGGTGCAAAAACATTTATTATCTTTCTTTGCCATTTGC TTAGAGGACTTCATGCAATCACAGACAGAGAAAAGGATCCTTTGATGTGTGAAAACATTCAAACCCATGG AAGGCATTGGAAAAATTGTTCTCGGTGGAAATTTATTTCCCAGGCTCTCCTAACGTTTCAGACACAAATA TTAGACGGTATTATGGACCAGTACCTAAGTAGACACTTTTTGTCTATCTTTTCGTCTGCTAAAGAGAGAA CATTGTGTAGGAGCTCTTTAGAGTTGCTCTTAGAAGCATACTTTTGTGGAAGAGTGGGAAGAAATAATCA TAAATTTATTTCACAGTTGATGTGTGACTACTTTTTCAAGTGTATGACTTGTAAAAGTGGGATTGGTGTA TTTGAGTTAGTGGATGACCATTTTGTAGAGTTGAATGTTGGTGTCACTGGCCTTCCTGTTTCAGATTCCA GGATCATAGCTGGTCTTGTGCTTCAGAAAGATTTTTCTGTGTACCGCCCAGCAGATGGTGACATGCGAAT GGTGATAGTAACAGAAACCATTCAGCCTCTTTTTTCCACTTCTGGATCAGAGTTTATTCTAAATTCAGAA GCACAGTTTCAGACATCTCAATTTTGGATTATGGAAAAGACAAAAGCAATAATGAAACATCTACATAGTC AGAATGTAAAATTGCTCATATCTAGTGTGAAACAACCAGATTTAGTTAGTTATTATGCAGGGGTGAATGG CATATCAGTGGTTGAGTGTTTATCATCAGAAGAAGTTTCTCTTATCCGGAGGATCATTGGTCTTTCTCCA TTTGTACCACCACAGGCCTTTTCGCAGTGTGAAATACCTAACACTGCTTTGGTGAAATTTTGTAAACCTC TTATCCTTAGATCCAAAAGATATGTTCATCTAGGCTTGATAAGCACATGTGCATTTATACCACACTCTAT AGTTCTTTGTGGACCAGTGCATGGTCTCATTGAACAACATGAGGATGCTTTACATGGAGCACTTAAAATG CTTCGGCAATTATTTAAAGACCTTGATCTAAATTACATGACACAAACCAATGACCAAAATGGCACTTCAA GTCTTTTTATTTATAAGAACAGTGGAGAAAGTTATCAAGCACCAGATCCGGGTAATGGCTCAATACAAAG GCCTTATCAGGACACAGTTGCAGAGAACAAAGATGCATTGGAAAAAACTCAAACATATTTAAAAGTACAT TCTAATTTGGTAATTCCAGATGTAGAATTAGAAACATATATTCCGTATTCAACCCCCACACTGACACCAA CAGATACATTCCAAACAGTTGAAACGCTGACATGTTTGTCTTTGGAAAGAAACAGGCTAACTGATTATTA TGAACCATTACTCAAGAACAATTCCACTGCTTATTCAACAAGGGGAAATAGAATAGAAATTTCTTACGAA AATTTACAGGTCACAAATATTACTAGAAAGGGAAGCATGTTACCAGTGAGCTGTAAGTTACCGAATATGG GTACTTCCCAGAGTTACCTTTCCTCATCTATGCCAGCTGGTTGTGTTTTGCCAGTAGGTGGTAATTTTGA TATCTTGTTACATTACTATCTTCTCAATTATGCCAAAAAATGCCATCAATCAGAAGAAACCATGGTTAGT ATGATAATAGCTAATGCACTTTTAGGCATTCCCAAAGTCCTTTATAAATCTAAAACAGGAAAGTACAGCT TTCCACATACATATATAAGAGCTGTCCATGCACTGCAAACCAATCAACCCTTGGTAAGCAGTCAGACAGG TTTGGAATCAGTAATGGGTAAATACCAGCTACTAACTTCAGTTCTTCAGTGTTTGACAAAAATATTAACC ATTGACATGGTAATCACTGTTAAGAGACACCCTCAGAAAGTTCACAATCAAGATTCAGAAGATGAACTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205501 protein sequence
Red=Cloning site Green=Tags(s) MLSSMAAAGSVKAALQVAEVLEAIVSCCVGPEGRQVLCTKPTGEVLLSRNGGRLLEALHLEHPIARMIVD CVSSHLKKTGDGAKTFIIFLCHLLRGLHAITDREKDPLMCENIQTHGRHWKNCSRWKFISQALLTFQTQI LDGIMDQYLSRHFLSIFSSAKERTLCRSSLELLLEAYFCGRVGRNNHKFISQLMCDYFFKCMTCKSGIGV FELVDDHFVELNVGVTGLPVSDSRIIAGLVLQKDFSVYRPADGDMRMVIVTETIQPLFSTSGSEFILNSE AQFQTSQFWIMEKTKAIMKHLHSQNVKLLISSVKQPDLVSYYAGVNGISVVECLSSEEVSLIRRIIGLSP FVPPQAFSQCEIPNTALVKFCKPLILRSKRYVHLGLISTCAFIPHSIVLCGPVHGLIEQHEDALHGALKM LRQLFKDLDLNYMTQTNDQNGTSSLFIYKNSGESYQAPDPGNGSIQRPYQDTVAENKDALEKTQTYLKVH SNLVIPDVELETYIPYSTPTLTPTDTFQTVETLTCLSLERNRLTDYYEPLLKNNSTAYSTRGNRIEISYE NLQVTNITRKGSMLPVSCKLPNMGTSQSYLSSSMPAGCVLPVGGNFDILLHYYLLNYAKKCHQSEETMVS MIIANALLGIPKVLYKSKTGKYSFPHTYIRAVHALQTNQPLVSSQTGLESVMGKYQLLTSVLQCLTKILT IDMVITVKRHPQKVHNQDSEDEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024685 |
| ORF Size | 2169 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024685.3, NP_078961.3 |
| RefSeq Size | 3583 bp |
| RefSeq ORF | 2172 bp |
| Locus ID | 79738 |
| UniProt ID | Q8TAM1 |
| MW | 80.8 kDa |
| Gene Summary | This gene is a member of the Bardet-Biedl syndrome (BBS) gene family. Bardet-Biedl syndrome is an autosomal recessive disorder characterized by progressive retinal degeneration, obesity, polydactyly, renal malformation and cognitive disability. The proteins encoded by BBS gene family members are structurally diverse and the similar phenotypes exhibited by mutations in BBS gene family members is likely due to their shared roles in cilia formation and function. Many BBS proteins localize to the basal bodies, ciliary axonemes, and pericentriolar regions of cells. BBS proteins may also be involved in intracellular trafficking via microtubule-related transport. The protein encoded by this gene is likely not a ciliary protein but rather has distant sequence homology to type II chaperonins. As a molecular chaperone, this protein may affect the folding or stability of other ciliary or basal body proteins. Inhibition of this protein's expression impairs ciliogenesis in preadipocytes. Mutations in this gene cause Bardet-Biedl syndrome type 10. [provided by RefSeq, Jan 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205501L3 | Lenti ORF clone of Human Bardet-Biedl syndrome 10 (BBS10), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC205501L4 | Lenti ORF clone of Human Bardet-Biedl syndrome 10 (BBS10), mGFP tagged |
CNY 7,700.00 |
|
| RG205501 | BBS10 (tGFP-tagged) - Human Bardet-Biedl syndrome 10 (BBS10) |
CNY 6,896.00 |
|
| SC108312 | BBS10 (untagged)-Human Bardet-Biedl syndrome 10 (BBS10) |
CNY 7,220.00 |
|
| SC318204 | BBS10 (untagged)-Human Bardet-Biedl syndrome 10 (BBS10) |
CNY 7,220.00 |
