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PIWIL1 (NM_004764) Human Tagged ORF Clone
CAT＃: RC205469
PIWIL1 (Myc-DDK-tagged)-Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1
ORF Plasmid: tGFP
"NM_004764" in other vectors (6)
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CNY 6,304.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CT80.1; HIWI; MIWI; PIWI |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205469 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGGGAGAGCCCGAGCCAGAGCCAGAGGAAGGGCCCGCGGTCAGGAGACAGCGCAGCTGGTGGGCT CCACTGCCAGTCAGCAACCTGGTTATATTCAGCCTAGGCCTCAGCCGCCACCAGCAGAGGGGGAATTATT TGGCCGTGGACGGCAGAGAGGAACAGCAGGAGGAACAGCCAAGTCACAAGGACTCCAGATATCTGCTGGA TTTCAGGAGTTATCGTTAGCAGAGAGAGGAGGTCGTCGTAGAGATTTTCATGATCTTGGTGTGAATACAA GGCAGAACCTAGACCATGTTAAAGAATCAAAAACAGGTTCTTCAGGCATTATAGTAAGGTTAAGCACTAA CCATTTCCGGCTGACATCCCGTCCCCAGTGGGCCTTATATCAGTATCACATTGACTATAACCCACTGATG GAAGCCAGAAGACTCCGTTCAGCTCTTCTTTTTCAACACGAAGATCTAATTGGAAAGTGTCATGCTTTTG ATGGAACGATATTATTTTTACCTAAAAGACTACAGCAAAAGGTTACTGAAGTTTTTAGTAAGACCCGGAA TGGAGAGGATGTGAGGATAACGATCACTTTAACAAATGAACTTCCACCTACATCACCAACTTGTTTGCAG TTCTATAATATTATTTTCAGGAGGCTTTTGAAAATCATGAATTTGCAACAAATTGGACGAAATTATTATA ACCCAAATGACCCAATTGATATTCCAAGTCACAGGTTGGTGATTTGGCCTGGCTTCACTACTTCCATCCT TCAGTATGAAAACAGCATCATGCTCTGCACTGACGTTAGCCATAAAGTCCTTCGAAGTGAGACTGTTTTG GATTTCATGTTCAACTTTTATCATCAGACAGAAGAACATAAATTTCAAGAACAAGTTTCCAAAGAACTAA TAGGTTTAGTTGTTCTTACCAAGTATAACAATAAGACATACAGAGTGGATGATATTGACTGGGACCAGAA TCCCAAGAGCACCTTTAAGAAAGCCGACGGCTCTGAAGTCAGCTTCTTAGAATACTACAGGAAGCAATAC AACCAAGAGATCACCGACTTGAAGCAGCCTGTCTTGGTCAGCCAGCCCAAGAGAAGGCGGGGCCCTGGGG GGACACTGCCAGGGCCTGCCATGCTCATTCCTGAGCTCTGCTATCTTACAGGTCTAACTGATAAAATGCG TAATGATTTTAACGTGATGAAAGACTTAGCCGTTCATACAAGACTAACTCCAGAGCAAAGGCAGCGTGAA GTGGGACGACTCATTGATTACATTCATAAAAACGATAATGTTCAAAGGGAGCTTCGAGACTGGGGTTTGA GCTTTGATTCCAACTTACTGTCCTTCTCAGGAAGAATTTTGCAAACAGAAAAGATTCACCAAGGTGGAAA AACATTTGATTACAATCCACAATTTGCAGATTGGTCCAAAGAAACAAGAGGTGCACCATTAATTAGTGTT AATCCACTAGATAACTGGCTGTTGATCTATACGCGAAGAAATTATGAAGCAGCCAATTCATTGATACAAA ATCTATTTAAAGTTACACCAGCCATGGGCATGCAAATGAAAAAAGCAATAATGATTGAAGTGGATGACAG AACTGAAGCCTACTTAAGAGTCTTACAGCAAAAGGTCACAGCAGACACCCAGATAGTTGTCTGTCTGTTG TCAAGTAATCGGAAGGACAAATACGATGCTATTAAAAAATACCCGTGTACAGATTGCCCTACCCCAAGTC AGTGTGTGGTGGCCCGAACCTTAGGCAAACAGCAAACTGTCATGGCCATTGCTACAAAGATTGCCCTACA GATGAACTGCAAGATGGGAGGAGAGCTCTGGAGGGTGGACATCCCCCTGAAGCTCGTGATGATCGTTGGC ATCGATTGTTACCATGACATGACAGCTGGGCGGAGGTCAATCGCAGGATTTGTTGCCAGCATCAATGAAG GGATGACCCGCTGGTTCTCACGCTGCATATTTCAGGATAGAGGACAGGAGCTGGTAGATGGGCTCAAAGT CTGCCTGCAAGCGGCTCTGAGGGCTTGGAATAGCTGCAATGAGTACATGCCCAGCCGGATCATCGTGTAC CGCGATGGCGTAGGAGACGGCCAGCTGAAAACACTGGTGAACTACGAAGTGCCACAGTTTTTGGATTGTC TAAAATCCATTGGTAGAGGTTACAACCCTAGACTAACGGTAATTGTGGTGAAGAAAAGAGTGAACACCAG ATTTTTTGCTCAGTCTGGAGGAAGACTTCAGAATCCACTTCCTGGAACAGTTATTGATGTAGAGGTTACC AGACCAGAATGGTATGACTTTTTTATCGTGAGCCAGGCTGTGAGAAGTGGTAGTGTTTCTCCCACACATT ACAATGTCATCTATGACAACAGCGGCCTGAAGCCAGACCACATACAGCGCTTGACCTACAAGCTGTGCCA CATCTATTACAACTGGCCAGGTGTCATTCGTGTTCCTGCTCCTTGCCAGTACGCCCACAAGCTGGCTTTT CTTGTTGGCCAGAGTATTCACAGAGAGCCAAATCTGTCACTGTCAAACCGCCTTTACTACCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205469 protein sequence
Red=Cloning site Green=Tags(s) MTGRARARARGRARGQETAQLVGSTASQQPGYIQPRPQPPPAEGELFGRGRQRGTAGGTAKSQGLQISAG FQELSLAERGGRRRDFHDLGVNTRQNLDHVKESKTGSSGIIVRLSTNHFRLTSRPQWALYQYHIDYNPLM EARRLRSALLFQHEDLIGKCHAFDGTILFLPKRLQQKVTEVFSKTRNGEDVRITITLTNELPPTSPTCLQ FYNIIFRRLLKIMNLQQIGRNYYNPNDPIDIPSHRLVIWPGFTTSILQYENSIMLCTDVSHKVLRSETVL DFMFNFYHQTEEHKFQEQVSKELIGLVVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQY NQEITDLKQPVLVSQPKRRRGPGGTLPGPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQRE VGRLIDYIHKNDNVQRELRDWGLSFDSNLLSFSGRILQTEKIHQGGKTFDYNPQFADWSKETRGAPLISV NPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGMQMKKAIMIEVDDRTEAYLRVLQQKVTADTQIVVCLL SSNRKDKYDAIKKYPCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDIPLKLVMIVG IDCYHDMTAGRRSIAGFVASINEGMTRWFSRCIFQDRGQELVDGLKVCLQAALRAWNSCNEYMPSRIIVY RDGVGDGQLKTLVNYEVPQFLDCLKSIGRGYNPRLTVIVVKKRVNTRFFAQSGGRLQNPLPGTVIDVEVT RPEWYDFFIVSQAVRSGSVSPTHYNVIYDNSGLKPDHIQRLTYKLCHIYYNWPGVIRVPAPCQYAHKLAF LVGQSIHREPNLSLSNRLYYL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004764 |
| ORF Size | 2583 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004764.2 |
| RefSeq Size | 3591 bp |
| RefSeq ORF | 2586 bp |
| Locus ID | 9271 |
| UniProt ID | Q96J94 |
| Protein Pathways | Dorso-ventral axis formation |
| MW | 98.5 kDa |
| Gene Summary | This gene encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205469L1 | Lenti ORF clone of Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1, Myc-DDK-tagged |
CNY 8,704.00 |
|
| RC205469L2 | Lenti ORF clone of Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1, mGFP tagged |
CNY 8,704.00 |
|
| RC205469L3 | Lenti ORF clone of Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1, Myc-DDK-tagged |
CNY 8,704.00 |
|
| RC205469L4 | Lenti ORF clone of Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1, mGFP tagged |
CNY 8,704.00 |
|
| RG205469 | PIWIL1 (tGFP-tagged) - Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1 |
CNY 7,904.00 |
|
| SC316969 | PIWIL1 (untagged)-Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1 |
CNY 6,312.00 |
