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PIWIL1 (NM_004764) Human Untagged Clone
CAT＃: SC316969
PIWIL1 (untagged)-Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1
CNY 6,312.00
CNY 7,220.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CT80.1; HIWI; MIWI; PIWI |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_004764 edited
GTTGGCCTCGGGCTGAGGTGCAAGGACCAGGACTAGGGCGAGGGCAGCGGTCCAAGAAAT AGAAAACAATGACTGGGAGAGCCCGAGCCAGAGCCAGAGGAAGGGCCCGCGGTCAGGAGA CAGCGCAGCTGGTGGGCTCCACTGCCAGTCAGCAACCTGGTTATATTCAGCCTAGGCCTC AGCCGCCACCAGCAGAGGGGGAATTATTTGGCCGTGGACGGCAGAGAGGAACAGCAGGAG GAACAGCCAAGTCACAAGGACTCCAGATATCTGCTGGATTTCAGGAGTTATCGTTAGCAG AGAGAGGAGGTCGTCGTAGAGATTTTCATGATCTTGGTGTGAATACAAGGCAGAACCTAG ACCATGTTAAAGAATCAAAAACAGGTTCTTCAGGCATTATAGTAAGGTTAAGCACTAACC ATTTCCGGCTGACATCCCGTCCCCAGTGGGCCTTATATCAGTATCACATTGACTATAACC CACTGATGGAAGCCAGAAGACTCCGTTCAGCTCTTCTTTTTCAACACGAAGATCTAATTG GAAAGTGTCATGCTTTTGATGGAACGATATTATTTTTACCTAAAAGACTACAGCAAAAGG TTACTGAAGTTTTTAGTAAGACCCGGAATGGAGAGGATGTGAGGATAACGATCACTTTAA CAAATGAACTTCCACCTACATCACCAACTTGTTTGCAGTTCTATAATATTATTTTCAGGA GGCTTTTGAAAATCATGAATTTGCAACAAATTGGACGAAATTATTATAACCCAAATGACC CAATTGATATTCCAAGTCACAGGTTGGTGATTTGGCCTGGCTTCACTACTTCCATCCTTC AGTATGAAAACAGCATCATGCTCTGCACTGACGTTAGCCATAAAGTCCTTCGAAGTGAGA CTGTTTTGGATTTCATGTTCAACTTTTATCATCAGACAGAAGAACATAAATTTCAAGAAC AAGTTTCCAAAGAACTAATAGGTTTAGTTGTTCTTACCAAGTATAACAATAAGACATACA GAGTGGATGATATTGACTGGGACCAGAATCCCAAGAGCACCTTTAAGAAAGCCGACGGCT CTGAAGTCAGCTTCTTAGAATACTACAGGAAGCAATACAACCAAGAGATCACCGACTTGA AGCAGCCTGTCTTGGTCAGCCAGCCCAAGAGAAGGCGGGGCCCTGGGGGGACACTGCCAG GGCCTGCCATGCTCATTCCTGAGCTCTGCTATCTTACAGGTCTAACTGATAAAATGCGTA ATGATTTTAACGTGATGAAAGACTTAGCCGTTCATACAAGACTAACTCCAGAGCAAAGGC AGCGTGAAGTGGGACGACTCATTGATTACATTCATAAAAACGATAATGTTCAAAGGGAGC TTCGAGACTGGGGTTTGAGCTTTGATTCCAACTTACTGTCCTTCTCAGGAAGAATTTTGC AAACAGAAAAGATTCACCAAGGTGGAAAAACATTTGATTACAATCCACAATTTGCAGATT GGTCCAAAGAAACAAGAGGTGCACCATTAATTAGTGTTAATCCACTAGATAACTGGCTGT TGATCTATACGCGAAGAAATTATGAAGCAGCCAATTCATTGATACAAAATCTATTTAAAG TTACACCAGCCATGGGCATGCAAATGAAAAAAGCAATAATGATTGAAGTGGATGACAGAA CTGAAGCCTACTTAAGAGTCTTACAGCAAAAGGTCACAGCAGACACCCAGATAGTTGTCT GTCTGTTGTCAAGTAATCGGAAGGACAAATACGATGCTATTAAAAAATACCCGTGTACAG ATTGCCCTACCCCAAGTCAGTGTGTGGTGGCCCGAACCTTAGGCAAACAGCAAACTGTCA TGGCCATTGCTACAAAGATTGCCCTACAGATGAACTGCAAGATGGGAGGAGAGCTCTGGA GGGTGGACATCCCCCTGAAGCTCGTGATGATCGTTGGCATCGATTGTTACCATGACATGA CAGCTGGGCGGAGGTCAATCGCAGGATTTGTTGCCAGCATCAATGAAGGGATGACCCGCT GGTTCTCACGCTGCATATTTCAGGATAGAGGACAGGAGCTGGTAGATGGGCTCAAAGTCT GCCTGCAAGCGGCTCTGAGGGCTTGGAATAGCTGCAATGAGTACATGCCCAGCCGGATCA TCGTGTACCGCGATGGCGTAGGAGACGGCCAGCTGAAAACACTGGTGAACTACGAAGTGC CACAGTTTTTGGATTGTCTAAAATCCATTGGTAGAGGTTACAACCCTAGACTAACGGTAA TTGTGGTGAAGAAAAGAGTGAACACCAGATTTTTTGCTCAGTCTGGAGGAAGACTTCAGA ATCCACTTCCTGGAACAGTTATTGATGTAGAGGTTACCAGACCAGAATGGTATGACTTTT TTATCGTGAGCCAGGCTGTGAGAAGTGGTAGTGTTTCTCCCACACATTACAATGTCATCT ATGACAACAGCGGCCTGAAGCCAGACCACATACAGCGCTTGACCTACAAGCTGTGCCACA TCTATTACAACTGGCCAGGTGTCATTCGTGTTCCTGCTCCTTGCCAGTACGCCCACAAGC TGGCTTTTCTTGTTGGCCAGAGTATTCACAGAGAGCCAAATCTGTCACTGTCAAACCGCC TTTACTACCTCTAACCTGCAGAAGACGATGCAGCCGCTTTTCTTTTTGAAATGACTTTGG GATTTTTTTAAGCTTTTATTTACTTTTTTTTTAACTGTTATCTTTCTGGATGAAACTTGG GAAGGGGATTAGGAGATCTAGCATTTTATTTCTAGCATTGCTATTCACCGGCTTCCTTAT TTTATACGTAAAAATTAAGATTTTATATTTTATCTTCTTGTTTCTCATAGATATTTTGTG AGCATTTTTTTGTTTATTTTGAAGAAATGTGGATAAGATACTTGGTAGTATAAAACAGAC TCTCTGAGAGTATTTGAAATGTGTTTGGAGATTTACTTAAACGTACTTTCAGGAGTGAGC AAGTCCTACTTATAAACCTATATTAACTTTATTTTTGAGATACCTGTTTTGAATTTAAAG GAGATAAGAGGCGTAAAGTAGGATGCTCACTACAACCATAGGTGGGGTTTCAGCTCATAT CTTAAAGATAAAAGGTACTATTATATAACCTATACACAAGATACAGGAGAAAATATGCTT GATTTTTATTTGGCAGGGGGGCTAGGTTGTATGGGAGTAAAAAAAACATTGAAAATTTTT AAATTGTCCAAAGAAACATTTTAAGACTCTTTAACAAAAAAGGCCATGAGTAAATCTCTA TATTAACATTACTATTTATTTTGTTTTGGAACTGGGACATGATTCTATTTGTTATAAAAT AAAATTGATGTGATTGTCAAAAAAAAAAAAAAAA >OriGene 5' read for NM_004764 unedited
GGGTTCACATTTCTAAACGACTTATATAGGCGACCGCATAACTTCGTATAGCATACATTA TACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGGTTGGCC TCGGGCTGAGGTGCAAGGACCAGGACTAGGGCGAGGGCAGCGGTCCAAGAAATAGAAAAC AATGACTGGGAGAGCCCGAGCCAGAGCCAGAGGAAGGGCCCGCGGTCAGGAGACAGCGCA GCTGGTGGGCTCCACTGCCAGTCAGCAACCTGGTTATATTCAGCCTAGGCCTCAGCCGCC ACCAGCAGAGGGGGAATTATTTGGCCGTGGACGGCAGAGAGGAACAGCAGGAGGAACAGC CAAGTCACAAGGACTCCAGATATCTGCTGGATTTCAGGAGTTATCGTTAGCAGAGAGAGG AGGTCGTCGTAGAGATTTTCATGATCTTGGTGTGAATACAAGGCAGAACCTAGACCATGT TAAAGAATCAAAAACAGGTTCTTCAGGCATTATAGTAAGGTTAAGCACTAACCATTTCCG GCTGACATCCCGTCCCCAGTGGGCCTTATATCAGTATCACATTGACTATAACCCACTGAT GGAAGCCAGAAGACTCCGTTCAGCTCTTCTTTTTCAACACGAAGATCTAATTGGAAAGTG TCATGCTTTTGATGGAACGATATTATTTTTACCTAAAAGACTACAGCAAAAGGTTACTGA AGTTTTTAGTAAGACCCCGAATGGAGAGGATGTGAGGATAACGATCACTTTAACAAATGA ACTTCCACCTACATCACCAACTTGTTTGCAGGTCTATAATATTATTTTTCAGGAAGCTTT TTGAAATCATGAATTTGCCACANATTGGACGAAATTATTATTACCCAAATGACCCAATTG ATTTTCCAGTC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004764 |
| Insert Size | 3400 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to contain 2 SNPs compared with NM_004764.3. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004764.3, NP_004755.2 |
| RefSeq Size | 3416 bp |
| RefSeq ORF | 2586 bp |
| Locus ID | 9271 |
| UniProt ID | Q96J94 |
| Protein Pathways | Dorso-ventral axis formation |
| Gene Summary | This gene encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Androgen-Induced TMPRSS2:ERG Fusion in Nonmalignant Prostate Epithelial Cells
,Nuria Coll Bastus, Lara K. Boyd, Xueying Mao, Elzbieta Stankiewicz, Sakunthala C. Kudahetti, R. Tim D. Oliver, Daniel M. Berney, and Yong-Jie Lu,
Cancer Res., Dec 2010; 70: 9544 - 9548
[PIWIL1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205469 | PIWIL1 (Myc-DDK-tagged)-Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1 |
CNY 6,304.00 |
|
| RC205469L1 | Lenti ORF clone of Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1, Myc-DDK-tagged |
CNY 8,704.00 |
|
| RC205469L2 | Lenti ORF clone of Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1, mGFP tagged |
CNY 8,704.00 |
|
| RC205469L3 | Lenti ORF clone of Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1, Myc-DDK-tagged |
CNY 8,704.00 |
|
| RC205469L4 | Lenti ORF clone of Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1, mGFP tagged |
CNY 8,704.00 |
|
| RG205469 | PIWIL1 (tGFP-tagged) - Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1 |
CNY 7,904.00 |
