PIWIL1 (NM_004764) Human Untagged Clone

Name: PIWIL1 (NM_004764) Human Untagged Clone
SKU: SC316969
Price: 6312.00

CAT＃: SC316969

PIWIL1 (untagged)-Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1

Datasheet

SDS

"NM_004764" in other vectors (6)

CNY 6,312.00

CNY 7,220.00

货期*

现货

规格

Cited in 1 publication.

Product images

经常一起买 (4)

TA324094

Anti-PIWIL1 Rabbit Polyclonal Antibody

CNY 1,999.00
CNY 3,280.00

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CT80.1; HIWI; MIWI; PIWI
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for NM_004764 edited GTTGGCCTCGGGCTGAGGTGCAAGGACCAGGACTAGGGCGAGGGCAGCGGTCCAAGAAAT AGAAAACAATGACTGGGAGAGCCCGAGCCAGAGCCAGAGGAAGGGCCCGCGGTCAGGAGA CAGCGCAGCTGGTGGGCTCCACTGCCAGTCAGCAACCTGGTTATATTCAGCCTAGGCCTC AGCCGCCACCAGCAGAGGGGGAATTATTTGGCCGTGGACGGCAGAGAGGAACAGCAGGAG GAACAGCCAAGTCACAAGGACTCCAGATATCTGCTGGATTTCAGGAGTTATCGTTAGCAG AGAGAGGAGGTCGTCGTAGAGATTTTCATGATCTTGGTGTGAATACAAGGCAGAACCTAG ACCATGTTAAAGAATCAAAAACAGGTTCTTCAGGCATTATAGTAAGGTTAAGCACTAACC ATTTCCGGCTGACATCCCGTCCCCAGTGGGCCTTATATCAGTATCACATTGACTATAACC CACTGATGGAAGCCAGAAGACTCCGTTCAGCTCTTCTTTTTCAACACGAAGATCTAATTG GAAAGTGTCATGCTTTTGATGGAACGATATTATTTTTACCTAAAAGACTACAGCAAAAGG TTACTGAAGTTTTTAGTAAGACCCGGAATGGAGAGGATGTGAGGATAACGATCACTTTAA CAAATGAACTTCCACCTACATCACCAACTTGTTTGCAGTTCTATAATATTATTTTCAGGA GGCTTTTGAAAATCATGAATTTGCAACAAATTGGACGAAATTATTATAACCCAAATGACC CAATTGATATTCCAAGTCACAGGTTGGTGATTTGGCCTGGCTTCACTACTTCCATCCTTC AGTATGAAAACAGCATCATGCTCTGCACTGACGTTAGCCATAAAGTCCTTCGAAGTGAGA CTGTTTTGGATTTCATGTTCAACTTTTATCATCAGACAGAAGAACATAAATTTCAAGAAC AAGTTTCCAAAGAACTAATAGGTTTAGTTGTTCTTACCAAGTATAACAATAAGACATACA GAGTGGATGATATTGACTGGGACCAGAATCCCAAGAGCACCTTTAAGAAAGCCGACGGCT CTGAAGTCAGCTTCTTAGAATACTACAGGAAGCAATACAACCAAGAGATCACCGACTTGA AGCAGCCTGTCTTGGTCAGCCAGCCCAAGAGAAGGCGGGGCCCTGGGGGGACACTGCCAG GGCCTGCCATGCTCATTCCTGAGCTCTGCTATCTTACAGGTCTAACTGATAAAATGCGTA ATGATTTTAACGTGATGAAAGACTTAGCCGTTCATACAAGACTAACTCCAGAGCAAAGGC AGCGTGAAGTGGGACGACTCATTGATTACATTCATAAAAACGATAATGTTCAAAGGGAGC TTCGAGACTGGGGTTTGAGCTTTGATTCCAACTTACTGTCCTTCTCAGGAAGAATTTTGC AAACAGAAAAGATTCACCAAGGTGGAAAAACATTTGATTACAATCCACAATTTGCAGATT GGTCCAAAGAAACAAGAGGTGCACCATTAATTAGTGTTAATCCACTAGATAACTGGCTGT TGATCTATACGCGAAGAAATTATGAAGCAGCCAATTCATTGATACAAAATCTATTTAAAG TTACACCAGCCATGGGCATGCAAATGAAAAAAGCAATAATGATTGAAGTGGATGACAGAA CTGAAGCCTACTTAAGAGTCTTACAGCAAAAGGTCACAGCAGACACCCAGATAGTTGTCT GTCTGTTGTCAAGTAATCGGAAGGACAAATACGATGCTATTAAAAAATACCCGTGTACAG ATTGCCCTACCCCAAGTCAGTGTGTGGTGGCCCGAACCTTAGGCAAACAGCAAACTGTCA TGGCCATTGCTACAAAGATTGCCCTACAGATGAACTGCAAGATGGGAGGAGAGCTCTGGA GGGTGGACATCCCCCTGAAGCTCGTGATGATCGTTGGCATCGATTGTTACCATGACATGA CAGCTGGGCGGAGGTCAATCGCAGGATTTGTTGCCAGCATCAATGAAGGGATGACCCGCT GGTTCTCACGCTGCATATTTCAGGATAGAGGACAGGAGCTGGTAGATGGGCTCAAAGTCT GCCTGCAAGCGGCTCTGAGGGCTTGGAATAGCTGCAATGAGTACATGCCCAGCCGGATCA TCGTGTACCGCGATGGCGTAGGAGACGGCCAGCTGAAAACACTGGTGAACTACGAAGTGC CACAGTTTTTGGATTGTCTAAAATCCATTGGTAGAGGTTACAACCCTAGACTAACGGTAA TTGTGGTGAAGAAAAGAGTGAACACCAGATTTTTTGCTCAGTCTGGAGGAAGACTTCAGA ATCCACTTCCTGGAACAGTTATTGATGTAGAGGTTACCAGACCAGAATGGTATGACTTTT TTATCGTGAGCCAGGCTGTGAGAAGTGGTAGTGTTTCTCCCACACATTACAATGTCATCT ATGACAACAGCGGCCTGAAGCCAGACCACATACAGCGCTTGACCTACAAGCTGTGCCACA TCTATTACAACTGGCCAGGTGTCATTCGTGTTCCTGCTCCTTGCCAGTACGCCCACAAGC TGGCTTTTCTTGTTGGCCAGAGTATTCACAGAGAGCCAAATCTGTCACTGTCAAACCGCC TTTACTACCTCTAACCTGCAGAAGACGATGCAGCCGCTTTTCTTTTTGAAATGACTTTGG GATTTTTTTAAGCTTTTATTTACTTTTTTTTTAACTGTTATCTTTCTGGATGAAACTTGG GAAGGGGATTAGGAGATCTAGCATTTTATTTCTAGCATTGCTATTCACCGGCTTCCTTAT TTTATACGTAAAAATTAAGATTTTATATTTTATCTTCTTGTTTCTCATAGATATTTTGTG AGCATTTTTTTGTTTATTTTGAAGAAATGTGGATAAGATACTTGGTAGTATAAAACAGAC TCTCTGAGAGTATTTGAAATGTGTTTGGAGATTTACTTAAACGTACTTTCAGGAGTGAGC AAGTCCTACTTATAAACCTATATTAACTTTATTTTTGAGATACCTGTTTTGAATTTAAAG GAGATAAGAGGCGTAAAGTAGGATGCTCACTACAACCATAGGTGGGGTTTCAGCTCATAT CTTAAAGATAAAAGGTACTATTATATAACCTATACACAAGATACAGGAGAAAATATGCTT GATTTTTATTTGGCAGGGGGGCTAGGTTGTATGGGAGTAAAAAAAACATTGAAAATTTTT AAATTGTCCAAAGAAACATTTTAAGACTCTTTAACAAAAAAGGCCATGAGTAAATCTCTA TATTAACATTACTATTTATTTTGTTTTGGAACTGGGACATGATTCTATTTGTTATAAAAT AAAATTGATGTGATTGTCAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_004764 unedited GGGTTCACATTTCTAAACGACTTATATAGGCGACCGCATAACTTCGTATAGCATACATTA TACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGGTTGGCC TCGGGCTGAGGTGCAAGGACCAGGACTAGGGCGAGGGCAGCGGTCCAAGAAATAGAAAAC AATGACTGGGAGAGCCCGAGCCAGAGCCAGAGGAAGGGCCCGCGGTCAGGAGACAGCGCA GCTGGTGGGCTCCACTGCCAGTCAGCAACCTGGTTATATTCAGCCTAGGCCTCAGCCGCC ACCAGCAGAGGGGGAATTATTTGGCCGTGGACGGCAGAGAGGAACAGCAGGAGGAACAGC CAAGTCACAAGGACTCCAGATATCTGCTGGATTTCAGGAGTTATCGTTAGCAGAGAGAGG AGGTCGTCGTAGAGATTTTCATGATCTTGGTGTGAATACAAGGCAGAACCTAGACCATGT TAAAGAATCAAAAACAGGTTCTTCAGGCATTATAGTAAGGTTAAGCACTAACCATTTCCG GCTGACATCCCGTCCCCAGTGGGCCTTATATCAGTATCACATTGACTATAACCCACTGAT GGAAGCCAGAAGACTCCGTTCAGCTCTTCTTTTTCAACACGAAGATCTAATTGGAAAGTG TCATGCTTTTGATGGAACGATATTATTTTTACCTAAAAGACTACAGCAAAAGGTTACTGA AGTTTTTAGTAAGACCCCGAATGGAGAGGATGTGAGGATAACGATCACTTTAACAAATGA ACTTCCACCTACATCACCAACTTGTTTGCAGGTCTATAATATTATTTTTCAGGAAGCTTT TTGAAATCATGAATTTGCCACANATTGGACGAAATTATTATTACCCAAATGACCCAATTG ATTTTCCAGTC
Restriction Sites	NotI-NotI
ACCN	NM_004764
Insert Size	3400 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	The ORF of this clone has been fully sequenced and found to contain 2 SNPs compared with NM_004764.3.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_004764.3, NP_004755.2
RefSeq Size	3416 bp
RefSeq ORF	2586 bp
Locus ID	9271
UniProt ID	Q96J94
Protein Pathways	Dorso-ventral axis formation
Gene Summary	This gene encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010] Transcript Variant: This variant (1) encodes the longer isoform (1).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this cDNA Clones has been cited in the following citations:
Androgen-Induced TMPRSS2:ERG Fusion in Nonmalignant Prostate Epithelial Cells ,Nuria Coll Bastus, Lara K. Boyd, Xueying Mao, Elzbieta Stankiewicz, Sakunthala C. Kudahetti, R. Tim D. Oliver, Daniel M. Berney, and Yong-Jie Lu, Cancer Res., Dec 2010; 70: 9544 - 9548 [PIWIL1]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC205469	PIWIL1 (Myc-DDK-tagged)-Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,304.00
RC205469L1	Lenti ORF clone of Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,704.00
RC205469L2	Lenti ORF clone of Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,704.00
RC205469L3	Lenti ORF clone of Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,704.00
RC205469L4	Lenti ORF clone of Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,704.00
RG205469	PIWIL1 (tGFP-tagged) - Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,904.00

其它PIWIL1产品

Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies