CBX8 (NM_020649) Human Tagged ORF Clone

CAT#: RC203417

CBX8 (Myc-DDK-tagged)-Human chromobox homolog 8 (CBX8)

ORF Plasmid: DDK tGFP



  "NM_020649" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


CBX8 mouse monoclonal antibody, clone OTI3D1 (formerly 3D1)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms PC3; RC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203417 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTTTCAGCGGTGGGGGAGCGGGTGTTCGCGGCCGAAGCCCTCCTGAAGCGGCGCATACGGAAAG
GACGCATGGAATACCTCGTGAAATGGAAGGGATGGTCGCAGAAGTACAGCACATGGGAACCGGAGGAAAA
CATCCTGGATGCTCGCTTGCTCGCAGCCTTTGAGGAAAGGGAAAGAGAGATGGAGCTCTATGGCCCCAAA
AAGCGTGGACCCAAGCCCAAAACCTTCCTCCTCAAAGCGCAGGCCAAGGCAAAGGCCAAAACTTACGAGT
TTCGAAGTGACTCAGCCAGGGGCATCCGGATCCCCTACCCTGGCCGCTCGCCCCAGGACCTGGCCTCCAC
TTCCCGGGCCCGGGAGGGCCTTCGAAACATGGGTTTGTCCCCGCCAGCGAGCAGCACCAGCACCAGCAGC
ACCTGCCGCGCAGAGGCCCCTCGGGACCGGGACCGAGACCGGGATAGGGACCGGGAGCGGGATCGAGAAA
GGGAGAGGGAGCGAGAGAGGGAGCGGGAACGTGAGAGGGAACGAGAGCGGGGTACCAGCAGAGTGGATGA
CAAGCCCAGCTCACCGGGGGACAGCTCGAAGAAGCGAGGCCCCAAGCCCCGGAAGGAGCTCCCGGACCCC
TCACAGAGGCCCTTAGGCGAACCCAGCGCCGGCCTCGGAGAGTACCTCAAGGGCAGGAAGCTGGACGACA
CCCCTTCCGGGGCAGGAAAGTTTCCAGCCGGCCACAGTGTGATCCAGCTGGCCCGAAGACAGGACTCGGA
CCTGGTGCAGTGTGGTGTGACCAGCCCTAGCTCAGCTGAGGCCACGGGCAAACTGGCTGTGGACACCTTC
CCGGCCAGGGTGATAAAGCACAGGGCTGCCTTCCTGGAGGCCAAAGGCCAGGGTGCCCTAGATCCCAATG
GCACCCGGGTCCGACATGGCTCAGGCCCCCCCAGCTCTGGGGGGGGCCTGTACCGGGACATGGGGGCCCA
GGGGGGAAGGCCCTCCCTCATCGCCAGGATCCCTGTGGCCAGAATCCTGGGGGACCCGGAGGAAGAGTCC
TGGAGCCCCTCCCTGACTAACCTGGAGAAGGTGGTGGTCACGGACGTGACCTCAAACTTTTTGACCGTCA
CCATTAAGGAAAGTAACACGGACCAAGGCTTTTTTAAAGAGAAAAGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203417 protein sequence
Red=Cloning site Green=Tags(s)

MELSAVGERVFAAEALLKRRIRKGRMEYLVKWKGWSQKYSTWEPEENILDARLLAAFEEREREMELYGPK
KRGPKPKTFLLKAQAKAKAKTYEFRSDSARGIRIPYPGRSPQDLASTSRAREGLRNMGLSPPASSTSTSS
TCRAEAPRDRDRDRDRDRERDRERERERERERERERERERGTSRVDDKPSSPGDSSKKRGPKPRKELPDP
SQRPLGEPSAGLGEYLKGRKLDDTPSGAGKFPAGHSVIQLARRQDSDLVQCGVTSPSSAEATGKLAVDTF
PARVIKHRAAFLEAKGQGALDPNGTRVRHGSGPPSSGGGLYRDMGAQGGRPSLIARIPVARILGDPEEES
WSPSLTNLEKVVVTDVTSNFLTVTIKESNTDQGFFKEKR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_020649
ORF Size 1167 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_020649.3
RefSeq Size 1561 bp
RefSeq ORF 1170 bp
Locus ID 57332
UniProt ID Q9HC52
Domains CHROMO
Protein Families Transcription Factors
MW 43.4 kDa
Gene Summary Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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