CBX8 (NM_020649) Human Tagged ORF Clone
CAT#: RG203417
- TrueORF®
CBX8 (tGFP-tagged) - Human chromobox homolog 8 (CBX8)
ORF Plasmid: DDK
"NM_020649" in other vectors (6)
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CNY 5,256.00
Cited in 2 publications. |
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | PC3; RC1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG203417 representing NM_020649
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTTTCAGCGGTGGGGGAGCGGGTGTTCGCGGCCGAAGCCCTCCTGAAGCGGCGCATACGGAAAG GACGCATGGAATACCTCGTGAAATGGAAGGGATGGTCGCAGAAGTACAGCACATGGGAACCGGAGGAAAA CATCCTGGATGCTCGCTTGCTCGCAGCCTTTGAGGAAAGGGAAAGAGAGATGGAGCTCTATGGCCCCAAA AAGCGTGGACCCAAGCCCAAAACCTTCCTCCTCAAAGCGCAGGCCAAGGCAAAGGCCAAAACTTACGAGT TTCGAAGTGACTCAGCCAGGGGCATCCGGATCCCCTACCCTGGCCGCTCGCCCCAGGACCTGGCCTCCAC TTCCCGGGCCCGGGAGGGCCTTCGAAACATGGGTTTGTCCCCGCCAGCGAGCAGCACCAGCACCAGCAGC ACCTGCCGCGCAGAGGCCCCTCGGGACCGGGACCGAGACCGGGATAGGGACCGGGAGCGGGATCGAGAAA GGGAGAGGGAGCGAGAGAGGGAGCGGGAACGTGAGAGGGAACGAGAGCGGGGTACCAGCAGAGTGGATGA CAAGCCCAGCTCACCGGGGGACAGCTCGAAGAAGCGAGGCCCCAAGCCCCGGAAGGAGCTCCCGGACCCC TCACAGAGGCCCTTAGGCGAACCCAGCGCCGGCCTCGGAGAGTACCTCAAGGGCAGGAAGCTGGACGACA CCCCTTCCGGGGCAGGAAAGTTTCCAGCCGGCCACAGTGTGATCCAGCTGGCCCGAAGACAGGACTCGGA CCTGGTGCAGTGTGGTGTGACCAGCCCTAGCTCAGCTGAGGCCACGGGCAAACTGGCTGTGGACACCTTC CCGGCCAGGGTGATAAAGCACAGGGCTGCCTTCCTGGAGGCCAAAGGCCAGGGTGCCCTAGATCCCAATG GCACCCGGGTCCGACATGGCTCAGGCCCCCCCAGCTCTGGGGGGGGCCTGTACCGGGACATGGGGGCCCA GGGGGGAAGGCCCTCCCTCATCGCCAGGATCCCTGTGGCCAGAATCCTGGGGGACCCGGAGGAAGAGTCC TGGAGCCCCTCCCTGACTAACCTGGAGAAGGTGGTGGTCACGGACGTGACCTCAAACTTTTTGACCGTCA CCATTAAGGAAAGTAACACGGACCAAGGCTTTTTTAAAGAGAAAAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203417 representing NM_020649
Red=Cloning site Green=Tags(s) MELSAVGERVFAAEALLKRRIRKGRMEYLVKWKGWSQKYSTWEPEENILDARLLAAFEEREREMELYGPK KRGPKPKTFLLKAQAKAKAKTYEFRSDSARGIRIPYPGRSPQDLASTSRAREGLRNMGLSPPASSTSTSS TCRAEAPRDRDRDRDRDRERDRERERERERERERERERERGTSRVDDKPSSPGDSSKKRGPKPRKELPDP SQRPLGEPSAGLGEYLKGRKLDDTPSGAGKFPAGHSVIQLARRQDSDLVQCGVTSPSSAEATGKLAVDTF PARVIKHRAAFLEAKGQGALDPNGTRVRHGSGPPSSGGGLYRDMGAQGGRPSLIARIPVARILGDPEEES WSPSLTNLEKVVVTDVTSNFLTVTIKESNTDQGFFKEKR TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020649 |
ORF Size | 1167 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020649.3 |
RefSeq Size | 1542 bp |
RefSeq ORF | 1170 bp |
Locus ID | 57332 |
UniProt ID | Q9HC52 |
Domains | CHROMO |
Protein Families | Transcription Factors |
Gene Summary | Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.[UniProtKB/Swiss-Prot Function] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
CBX8 acts as an independent RNA-binding protein to regulate the maturation of miR-378a-3p in colon cancer cells
,Song, X;Ning, W;Niu, J;Zhang, G;Liu, H;Zhou, L;,
Human cell
,PubMed ID 33417156
[CBX8]
|
Synergistic effects of miR-708-5p and miR-708-3p accelerate the progression of osteoporosis
,Wang, R;Feng, Y;Xu, H;Huang, H;Zhao, S;Wang, Y;Li, H;Cao, J;Xu, G;Huang, S;,
The Journal of international medical research
,PubMed ID 33322976
[CBX8]
|
Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203417 | CBX8 (Myc-DDK-tagged)-Human chromobox homolog 8 (CBX8) |
CNY 3,656.00 |
|
RC203417L1 | Lenti ORF clone of Human chromobox homolog 8 (CBX8), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC203417L2 | Lenti ORF clone of Human chromobox homolog 8 (CBX8), mGFP tagged |
CNY 5,890.00 |
|
RC203417L3 | Lenti ORF clone of Human chromobox homolog 8 (CBX8), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC203417L4 | Lenti ORF clone of Human chromobox homolog 8 (CBX8), mGFP tagged |
CNY 6,056.00 |
|
SC319326 | CBX8 (untagged)-Human chromobox homolog 8 (CBX8) |
CNY 3,656.00 |