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ALDH1A1 (NM_000689) Human Tagged ORF Clone
CAT＃: RC200723
ALDH1A1 (Myc-DDK-tagged)-Human aldehyde dehydrogenase 1 family, member A1 (ALDH1A1)
ORF Plasmid: tGFP
"NM_000689" in other vectors (6)
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CNY 3,728.00
CNY 4,560.00
| Cited in 4 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ALDC; ALDH-E1; ALDH1; ALDH11; HEL-9; HEL-S-53e; HEL12; PUMB1; RALDH1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200723 representing NM_000689
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCATCCTCAGGCACGCCAGACTTACCTGTCCTACTCACCGATTTGAAGATTCAATATACTAAGATCT TCATAAACAATGAATGGCATGATTCAGTGAGTGGCAAGAAATTTCCTGTCTTTAATCCTGCAACTGAGGA GGAGCTCTGCCAGGTAGAAGAAGGAGATAAGGAGGATGTTGACAAGGCAGTGAAGGCCGCAAGACAGGCT TTTCAGATTGGATCCCCGTGGCGTACTATGGATGCTTCCGAGAGGGGGCGACTATTATACAAGTTGGCTG ATTTAATCGAAAGAGATCGTCTGCTGCTGGCGACAATGGAGTCAATGAATGGTGGAAAACTCTATTCCAA TGCATATCTGAATGATTTAGCAGGCTGCATCAAAACATTGCGCTACTGTGCAGGTTGGGCTGACAAGATC CAGGGCCGTACAATACCAATTGATGGAAATTTTTTTACATATACAAGACATGAACCTATTGGTGTATGTG GCCAAATCATTCCTTGGAATTTCCCGTTGGTTATGCTCATTTGGAAGATAGGGCCTGCACTGAGCTGTGG AAACACAGTGGTTGTCAAACCAGCAGAGCAAACTCCTCTCACTGCTCTCCACGTGGCATCTTTAATAAAA GAGGCAGGGTTTCCTCCTGGAGTAGTGAATATTGTTCCTGGTTATGGGCCTACAGCAGGGGCAGCCATTT CTTCTCACATGGATATAGACAAAGTAGCCTTCACAGGATCAACAGAGGTTGGCAAGTTGATCAAAGAAGC TGCCGGGAAAAGCAATCTGAAGAGGGTGACCCTGGAGCTTGGAGGAAAGAGCCCTTGCATTGTGTTAGCT GATGCCGACTTGGACAATGCTGTTGAATTTGCACACCATGGGGTATTCTACCACCAGGGCCAGTGTTGTA TAGCCGCATCCAGGATTTTTGTGGAAGAATCAATTTATGATGAGTTTGTTCGAAGGAGTGTTGAGCGGGC TAAGAAGTATATCCTTGGAAATCCTCTGACCCCAGGAGTCACTCAAGGCCCTCAGATTGACAAGGAACAA TATGATAAAATACTTGACCTCATTGAGAGTGGGAAGAAAGAAGGGGCCAAACTGGAATGTGGAGGAGGCC CGTGGGGGAATAAAGGCTACTTTGTCCAGCCCACAGTGTTCTCTAATGTTACAGATGAGATGCGCATTGC CAAAGAGGAGATTTTTGGACCAGTGCAGCAAATCATGAAGTTTAAATCTTTAGATGACGTGATCAAAAGA GCAAACAATACTTTCTATGGCTTATCAGCAGGAGTGTTTACCAAAGACATTGATAAAGCCATAACAATCT CCTCTGCTCTGCAGGCAGGAACAGTGTGGGTGAATTGCTATGGCGTGGTAAGTGCCCAGTGCCCCTTTGG TGGATTCAAGATGTCTGGAAATGGAAGAGAACTGGGAGAGTACGGTTTCCATGAATATACAGAGGTCAAA ACAGTCACAGTGAAAATCTCTCAGAAGAACTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200723 representing NM_000689
Red=Cloning site Green=Tags(s) MSSSGTPDLPVLLTDLKIQYTKIFINNEWHDSVSGKKFPVFNPATEEELCQVEEGDKEDVDKAVKAARQA FQIGSPWRTMDASERGRLLYKLADLIERDRLLLATMESMNGGKLYSNAYLNDLAGCIKTLRYCAGWADKI QGRTIPIDGNFFTYTRHEPIGVCGQIIPWNFPLVMLIWKIGPALSCGNTVVVKPAEQTPLTALHVASLIK EAGFPPGVVNIVPGYGPTAGAAISSHMDIDKVAFTGSTEVGKLIKEAAGKSNLKRVTLELGGKSPCIVLA DADLDNAVEFAHHGVFYHQGQCCIAASRIFVEESIYDEFVRRSVERAKKYILGNPLTPGVTQGPQIDKEQ YDKILDLIESGKKEGAKLECGGGPWGNKGYFVQPTVFSNVTDEMRIAKEEIFGPVQQIMKFKSLDDVIKR ANNTFYGLSAGVFTKDIDKAITISSALQAGTVWVNCYGVVSAQCPFGGFKMSGNGRELGEYGFHEYTEVK TVTVKISQKNS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000689 |
| ORF Size | 1503 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000689.5 |
| RefSeq Size | 2116 bp |
| RefSeq ORF | 1506 bp |
| Locus ID | 216 |
| UniProt ID | P00352 |
| Domains | aldedh |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS |
| Protein Pathways | Metabolic pathways, Retinol metabolism |
| MW | 54.7 kDa |
| Gene Summary | The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
ALDH1A1 overexpression in melanoma cells promotes tumor angiogenesis by activating the IL-8/Notch signaling cascade
,null,
International Journal of Molecular Medicine
,PubMed ID 35656893
[ALDH1A1]
|
|
Stemness marker ALDH1A1 promotes tumor angiogenesis via retinoic acid/HIF-1α/VEGF signalling in MCF-7 breast cancer cells
,null,
Journal of Experimental & Clinical Cancer Research : CR
,PubMed ID 30541574
[ALDH1A1]
|
|
ALDH1A1 and HLTF modulate the activity of lysosomal autophagy inhibitors in cancer cells
,null,
Autophagy
,PubMed ID 28981387
[ALDH1A1]
|
|
Complex Behavior of ALDH1A1 and IGFBP1 in Liver Metastasis from a Colorectal Cancer
,Kim, JC;Ha, YJ;Tak, KH;Roh, SA;Kim, CW;Kim, TW;Kim, SK;Kim, SY;Cho, DH;Kim, YS;,
PLoS ONE
,PubMed ID 27152521
[ALDH1A1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200723L1 | Lenti ORF clone of Human aldehyde dehydrogenase 1 family, member A1 (ALDH1A1), Myc-DDK-tagged |
CNY 6,128.00 |
|
| RC200723L2 | Lenti ORF clone of Human aldehyde dehydrogenase 1 family, member A1 (ALDH1A1), mGFP tagged |
CNY 6,460.00 |
|
| RC200723L3 | Lenti ORF clone of Human aldehyde dehydrogenase 1 family, member A1 (ALDH1A1), Myc-DDK-tagged |
CNY 6,128.00 |
|
| RC200723L4 | Lenti ORF clone of Human aldehyde dehydrogenase 1 family, member A1 (ALDH1A1), mGFP tagged |
CNY 6,128.00 |
|
| RG200723 | ALDH1A1 (tGFP-tagged) - Human aldehyde dehydrogenase 1 family, member A1 (ALDH1A1) |
CNY 5,320.00 |
|
| SC321535 | ALDH1A1 (untagged)-Human aldehyde dehydrogenase 1 family, member A1 (ALDH1A1) |
CNY 3,736.00 |
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