RHEB (NM_005614) Human Tagged ORF Clone
CAT#: RC200307
RHEB (Myc-DDK-tagged)-Human Ras homolog enriched in brain (RHEB)
ORF Plasmid: tGFP
"NM_005614" in other vectors (6)
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CNY 3,600.00
CNY 3,705.00
Cited in 5 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | RHEB2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200307 representing NM_005614
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCAGTCCAAGTCCCGGAAGATCGCGATCCTGGGCTACCGGTCTGTGGGGAAATCCTCATTGACGA TTCAATTTGTTGAAGGCCAATTTGTGGACTCCTACGATCCAACCATAGAAAACACTTTTACAAAGTTGAT CACAGTAAATGGACAAGAATATCATCTTCAACTTGTAGACACAGCCGGGCAAGATGAATATTCTATCTTT CCTCAGACATACTCCATAGATATTAATGGCTATATTCTTGTGTATTCTGTTACATCAATCAAAAGTTTTG AAGTGATTAAAGTTATCCATGGCAAATTGTTGGATATGGTGGGGAAAGTACAAATACCTATTATGTTGGT TGGGAATAAGAAAGACCTGCATATGGAAAGGGTGATCAGTTATGAAGAAGGGAAAGCTTTGGCAGAATCT TGGAATGCAGCTTTTTTGGAATCTTCTGCTAAAGAAAATCAGACTGCTGTGGATGTTTTTCGAAGGATAA TTTTGGAGGCAGAAAAAATGGACGGGGCAGCTTCACAAGGCAAGTCTTCATGCTCGGTGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200307 representing NM_005614
Red=Cloning site Green=Tags(s) MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIF PQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAES WNAAFLESSAKENQTAVDVFRRIILEAEKMDGAASQGKSSCSVM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_005614 |
ORF Size | 552 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005614.4 |
RefSeq Size | 1396 bp |
RefSeq ORF | 555 bp |
Locus ID | 6009 |
UniProt ID | Q15382 |
Domains | ras, RAS, RHO, RAB |
Protein Pathways | Insulin signaling pathway, mTOR signaling pathway |
MW | 20.3 kDa |
Gene Summary | This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008] |
Citations (5)
The use of this cDNA Clones has been cited in the following citations: |
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Induction of GDNF and GFRα-1 Following AAV1-Rheb(S16H) Administration in the Hippocampus in vivo
,Yun, D;Jeon, MT;Kim, HJ;Moon, GJ;Lee, S;Ha, CM;Shin, M;Kim, SR;,
Exp Neurobiol
,PubMed ID 32408406
[RHEB]
|
Therapeutic Potential of AAV1-Rheb(S16H) Transduction Against Alzheimer's Disease
,Moon, GJ;Kim, S;Jeon, MT;Lee, KJ;Jang, IS;Nakamura, M;Kim, SR;,
J Clin Med
,PubMed ID 31766645
[RHEB]
|
Neurotrophic interactions between neurons and astrocytes following AAV1-Rheb(S16H) transduction in the hippocampus in vivo
,Jeon, MT;Moon, GJ;Kim, S;Choi, M;Oh, YS;Kim, DW;Kim, HJ;Lee, KJ;Choe, Y;Ha, CM;Jang, IS;Nakamura, M;McLean, C;Chung, WS;Shin, WH;Lee, SG;Kim, SR;,
Br. J. Pharmacol.
,PubMed ID 31658360
[RHEB]
|
AKT3 Gene Transfer Promotes Anabolic Reprogramming and Photoreceptor Neuroprotection in a Pre-clinical Model of Retinitis Pigmentosa
,McDougald, DS;Papp, TE;Zezulin, AU;Zhou, S;Bennett, J;,
Mol. Ther.
,PubMed ID 31043342
[RHEB]
|
Induction of GDNF and BDNF by hRheb(S16H) Transduction of SNpc Neurons: Neuroprotective Mechanisms of hRheb(S16H) in a Model of Parkinson's Disease
,Nam, JH;Leem, E;Jeon, MT;Jeong, KH;Park, JW;Jung, UJ;Kholodilov, N;Burke, RE;Jin, BK;Kim, SR;,
Mol. Neurobiol.
,PubMed ID 24859383
[RHEB]
|
Documents
Product Manuals |
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SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200307L1 | Lenti ORF clone of Human Ras homolog enriched in brain (RHEB), Myc-DDK-tagged |
CNY 6,000.00 |
|
RC200307L2 | Lenti ORF clone of Human Ras homolog enriched in brain (RHEB), mGFP tagged |
CNY 6,000.00 |
|
RC200307L3 | Lenti ORF clone of Human Ras homolog enriched in brain (RHEB), Myc-DDK-tagged |
CNY 6,000.00 |
|
RC200307L4 | Lenti ORF clone of Human Ras homolog enriched in brain (RHEB), mGFP tagged |
CNY 5,890.00 |
|
RG200307 | RHEB (tGFP-tagged) - Human Ras homolog enriched in brain (RHEB) |
CNY 5,200.00 |
|
SC116636 | RHEB (untagged)-Human Ras homolog enriched in brain (RHEB) |
CNY 3,600.00 |