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RHEB (NM_005614) Human Untagged Clone
CAT＃: SC116636
RHEB (untagged)-Human Ras homolog enriched in brain (RHEB)
CNY 3,600.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | RHEB2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_005614 edited
GAATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGGCCGCCGCGGTTGATGTGGTTG GGCCGGGGCTGAGGAGGCCGCCAAGATGCCGCAGTCCAAGTCCCGGAAGATCGCGATCCT GGGCTACCGGTCTGTGGGGAAATCCTCATTGACGATTCAATTTGTTGAAGGCCAATTTGT GGACTCCTACGATCCAACCATAGAAAACACTTTTACAAAGTTGATCACAGTAAATGGACA AGAATATCATCTTCAACTTGTAGACACAGCCGGGCAAGATGAATATTCTATCTTTCCTCA GACATACTCCATAGATATTAATGGCTATATTCTTGTGTATTCTGTTACATCAATCAAAAG TTTTGAAGTGATTAAAGTTATCCATGGCAAATTGTTGGATATGGTGGGGAAAGTACAAAT ACCTATTATGTTGGTTGGGAATAAGAAAGACCTGCATATGGAAAGGGTGATCAGTTATGA AGAAGGGAAAGCTTTGGCAGAATCTTGGAATGCAGCTTTTTTGGAATCTTCTGCTAAAGA AAATCAGACTGCTGTGGATGTTTTTCGAAGGATAATTTTGGAGGCAGAAAAAATGGACGG GGCAGCTTCACAAGGCAAGTCTTCATGCTCGGTGATGTGATTCTGCTGCAAAGCCTGAGG ACACTGGGAATATATTCTACCTGAAGAAGCAAACTGCCCGTTCTCCTTGAAGATAAACTA TGCTTCTTTTTTCTTCTGTTAACCTGAAAGATATCATTTGGGTCAGAGCTCCCCTCCCTT CAGATTATGTTAACTCTGAGTCTGTCCAAATGAGTTCACTTCCATTTTCAAATTTTAAGC AATCATATTTTCAATTTATATATTGTATTTCTTAATATTATGACCAAGAATTTTATCGGC ATTAATTTTTCAGTGTAGTTTGTTGTTTAAAATAATGTAATCATCAAAATGATGCATATT GTTACACTACTATTAACTAGGCTTCAGTATATCAGTGTTTATTTCATTGTGTTAAATGTA TACTTGTAAATAAAATAGCTGCAAACCTCAAAAAAAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_005614 unedited
TTTTGTAATACGACTCACTATAGGGCGGCACGCGAATTCGCACGAGGCCTCGTGCCGAAT TCGGCACGAGGCCGCCGCGGTTGATGTGGTTGGGCCGGGGCTGAGGAGGCCGCCAAGATG CCGCAGTCCAAGTCCCGGAAGATCGCGATCCTGGGCTACCGGTCTGTGGGGAAATCCTCA TTGACGATTCAATTTGTTGAAGGCCAATTTGTGGACTCCTACGATCCAACCATAGAAAAC ACTTTTACAAAGTTGATCACAGTAAATGGACAAGAATATCATCTTCAACTTGTAGACACA GCCGGGCAAGATGAATATTCTATCTTTCCTCAGACATACTCCATAGATATTAATGGCTAT ATTCTTGTGTATTCTGTTACATCAATCAAAAGTTTTGAAGTGATTAAAGTTATCCATGGC AAATTGTTGGATATGGTGGGGAAAGTACAAATACCTATTATGTTGGTTGGGAATAAGAAA GACCTGCATATGGAAAGGGTGATCAGTTATGAAGAAGGGAAAGCTTTGGCAGAATCTTGG AATGCAGCTTTTTTGGAATCTTCTGCTAAAGAAAATCAGACTGCTGTGGATGTTTTTCGA AGGATAATTTTGGAGGCAGAAAAAATGGACGGNGCAGCTTCACAAGGCAAGTCTTCATGC TCGGTGATGTGATTCTGCTGCAAAGCCTGAGGACACTGNGAATATATTCTACCTGAAGAA GCANACTGCCCGTTCTCCTTGAAGATAACTATGCTTCTTTNTTCTTCTGTTAACCTGAAA GAATCATTTGGGTCAGAGCTCCCCTTCCCTTCAGATATGTACCTCTGAGTCTGTCCAAAT GAGTCACTTCATTNTCAATTTAAGCATCATATTTCATTTATTATGGNATTCTTATATATG ACAAGATNTAT >OriGene 3' read for NM_005614 unedited
GGGGGCGGGGGNNGGTGTTNNGGTTCTGTGTGCCGNNTGCCGTTCCCCGACTCTGCCCCC GCGGCCCGCCCACTGACGATCCGTTTTTTTTTTTTTTTTTTGCAGTTTGCAGCTATTTTA TTTACAAGCATACATTTAACACAATGCAAATAAACACTGATATACTGAAGCCTAGTTAAT AGTAGTGTAACAATATGCATCATTTTGATGATTACATTATTTTAAACAACAAACTACACT GGAAAAATTAATGCCGATAAAATTCTTGGCCATAATATTAAGAAATACCATATATAACTT GAAAATATGATTGCTTAAAATTTGAAAATGGAAGTGAACTCATTTGGACAGACTCAGAGT TAACATAATCTGAATGGAGGGGAGCTCTGACCCAAATGATATCTTTCAGGTTAACAGAAG AAAAAAGAAGCATAGCTTATTTTCAAGGAGAACGGGCAGTTTGCTTTTTCAGGTAGAATA TATTCCCAGTGTCCTCACGCTTTGCAGCAGAATCACATCACCGAGCATGAACACTTGCCT TGTGAAGCTGCCCCGTCCATTTTTTCTGCCTCCAAAAATATCCCTCGAAAAACCATCCAC AGCACCCTGATTTTTTTTAGCCCAAACACTCCCAAAAAGCTGCATTCCCCGCATTCTGCC AACACCTTTCCCTTCCTCCATACCTGAATCCCCCTTTCCATAATGCAGGGCCTTTCCTTA TTTCCAACCCCACCATATTAGGCTATTTTGTACTTTTTCCCCCACCATATCCAACCCAAT CTGCCCCTTGGGAAAACCTTTCAATCCACTTCCCAAACTTTTTGATCTGATGTCACCAGC ACTTCCCCACGGAATATAACCCCTTCAACAACCCAAGGGAGCAAGGTTTCGAAGGCAAAC AAAGCAAAATTTCATCCTTGGCCCGGGCCGGGGTTCTAAAAAATTCGAACATGAATACTC T |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005614 |
| Insert Size | 1000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005614.2, NP_005605.1 |
| RefSeq Size | 1396 bp |
| RefSeq ORF | 555 bp |
| Locus ID | 6009 |
| UniProt ID | Q15382 |
| Domains | ras, RAS, RHO, RAB |
| Protein Pathways | Insulin signaling pathway, mTOR signaling pathway |
| Gene Summary | This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
In Vivo AAV1 Transduction With hRheb(S16H) Protects Hippocampal Neurons by BDNF Production
,Jeon, MT;Nam, JH;Shin, WH;Leem, E;Jeong, KH;Jung, UJ;Bae, YS;Jin, YH;Kholodilov, N;Burke, RE;Lee, SG;Jin, BK;Kim, SR;,
Mol. Ther.
,PubMed ID 25502903
[RHEB]
|
|
Formation of endothelial lumens requires a coordinated PKC-, Src-, Pak- and Raf-kinase-dependent signaling cascade downstream of Cdc42 activation
,Wonshill Koh, Kamakshi Sachidanandam, Amber N. Stratman, Anastasia Sacharidou, Anne M. Mayo, Eric A. Murphy, David A. Cheresh, and George E. Davis,
J. Cell Sci., Jun 2009; 122: 1812 - 1822.
[Rheb]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200307 | RHEB (Myc-DDK-tagged)-Human Ras homolog enriched in brain (RHEB) |
CNY 3,600.00 |
|
| RC200307L1 | Lenti ORF clone of Human Ras homolog enriched in brain (RHEB), Myc-DDK-tagged |
CNY 6,000.00 |
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| RC200307L2 | Lenti ORF clone of Human Ras homolog enriched in brain (RHEB), mGFP tagged |
CNY 6,000.00 |
|
| RC200307L3 | Lenti ORF clone of Human Ras homolog enriched in brain (RHEB), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC200307L4 | Lenti ORF clone of Human Ras homolog enriched in brain (RHEB), mGFP tagged |
CNY 5,890.00 |
|
| RG200307 | RHEB (tGFP-tagged) - Human Ras homolog enriched in brain (RHEB) |
CNY 5,200.00 |
